The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 1 Issue 1
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- Pages.25-32
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- 1988
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Studies on Temperature-sensitive Mutant of Sacchnyomycopsis lipolytica
Saccharomycopsis lipolytica의 온도감수성 변이에 관한 연구
Abstract
Properties of purified isocitrate lyase from Saccharomycopsis lipolytica ATCC 44601 and MX9-11RX8 temperature-sensitive mutant were investigated. Purified isocitrate lyase from temperature-sensitive mutant was indistinguishable from the wild type enzyme with respect to the isoelectric pH(5.3), the thermostability and Km value for threo-Ds-Isocitrate(about 0.2 mM). When isocitrate lyase induced by acetate minimal medium at 33
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