• 사상체질의학회지
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• 제22권4호
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• pp.56-64
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• 2010

1. Objectives 4 herbal formulas (Yanggyeoksanhwa-tang, Yeoldahanso-tang, Cheongsimyeonja-tang and Taeeumjowi-tang) were applied to investigate the anti-inflammatory activities. In many studies, plant-derived anti-inflammatory efficacies have been investigate for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory activities of 4 herbal formulas on LPS-stimulated RAW 264.7 cells. 2. Methods The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin(IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ were examined in the presence of the 4 herbal formulas in RAW 264.7 cells. The cells were incubated with LPS 1 ${\mu}g/mL$ and 4 herbal formulas for 18 hrs. The anti-inflammatory activity of 4 herbal formulas were investigate by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hrs following carrageenininduced paw edema in rats. 3. Results Yanggyeoksanhwa-tang and Cheongsimyeonja-tang showed inhibitory effect on $PGE_2$ production in LPS-stimulated RAW 264.7 cells and a reduction in carrageenin-induced paw edema on rats. Yanggyeoksanhwa-tang showed inhibitory effect on IL-6 in LPS-stimulated RAW 264.7 cells. 4 herbal formulas not affect on NO and TNF-${\alpha}$ inhibition in LPS-stimulated RAW 264.7 cells. 4. Conclusions These results suggested that Yanggyeoksanhwa-tang and Cheongsimyeonja-tang have anti-inflammatory activity.

• 대한한의학회지
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• 제32권2호
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• pp.102-117
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• 2011

Objectives: This study was performed to investigate the protective and treating efficacy of Pyeongwi-san, Ijin-tang, and Pyeongjin-tang extracts to the mice with gastric mucosal lesions induced from indomethacin. Methods: In order to verify protective and treating efficacy of Pyeongwi-san, Ijin-tang, and Pyeongjin-tang extracts to the mice with gastric mucosal lesions induced from indomethacin, I administered the extracts of these prescriptions to three group, and induced gastric mucosal lesion by indomethacin, and then I observed the gastric mucosal morphology of stomach, changes from stress resulting from HSP70, changes of gastro-protection (mucous barrier, COX-1). After I observed the anti-oxidant effect, and anti-inflammation effect (IKK mRNA, iNOS mRNA, COX-2 mRNA) in vitro, I induced gastric mucosal lesion by indomethacin, and administered the extracts of each prescriptions to three group, and then I observed the gastric mucosal morphology, anti-inflammation effect to mucosa (NF-${\kappa}$B, iNOS, COX-2) in vivo. Results & Conclusions: 1. Hemorrhagic erosion and damaged mucus secreting cell, positive responses to HSP70 were decreased in all the before-gastric-mucosal-lesion-induced groups, compared to non-extract administered group. The effects were good in the order of Pyeongwi-san extracts administered group, Pyeongjin-tang extracts administered group and Ijin-tang extracts administered group. 2. In all the before-gastric-mucosal-lesion-induced groups, gastro- protection functions (mucous barrier, COX-1) were significant. The effects were good in the order of Pyeongwi-san extracts administered group, Pyeongjin-tang extracts administered group and Ijin-tang extracts administered group. 3. Anti-oxidant effect was significant in Pyeongwi-san extracts, Ijin-tang extracts and Pyeongjin-tang extracts. The effects were good in the order of Pyeongjin-tang extracts, Pyeongwi-san extracts and Ijin-tang extracts. 4. The anti-inflammation effects in vitro were good in Pyeongwi-san extracts, Ijin-tang extracts and Pyeongjin-tang extracts. Especially Pyeongjin-tang extracts showed the most prominent results. Damaged mucus secreting cells and the positive reactions of NF-${\kappa}$B, iNOS, COX-2 in vivo were decreased in after-gastric-mucosal-lesion-induced groups compared to non-extract administered group. The effects were good in the order of Pyeongjin-tang extracts administered group, Pyeongwi-san extracts administered group and Ijin-tang extracts administered group. These results show that Pyeongwi-san, Ijin-tang and Pyeongjin-tang are effective on both in protecting and treating the gastric mucosal membrane. Pyeongwi-san is more effective than other prescriptions, in protecting gastric mucosal membrane, and Pyeongjin-tang is more effective in treating gastric mucosal lesion.

• 대한한방내과학회지
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• 제21권3호
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• pp.433-441
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• 2000

Objective : To analyze the effects of Yukmijihwang-tang, Taeksa-tang, Silbi-um on mesangial cell proliferation, fibronectin synthesis and MHC-class II expression. Methods : Laboratory studies were performed with the method of surface enzyme immunoassays or flow cytometry after addition of peripheral blood mononuclear cells(PBMC) supernatants treated with medications using the cultured human mesangial cells. Results : 1. Silbi-um produces more suppressive effect than control group and hydrocortisone group on the mesangial cell proliferation. In Yukmijihwang-tang, Taeksa-tang and Silbi-um, mesangial cell proliferation significantly decreased than in hydrocortisone group 2. In the 'without fetal bovine serum' study, Yukmijihwang-tang take more suppressive effect than Control group on the fibronectin synthesis. In the 'with fetal bovine serum' study, Yukmijihwang-tang, Taeksa-tang, Silbi-um all have suppressive effect, but it hasn' t any statistical significance. 3. Yukmijihwang-tang, Taeksa-tang, Silbi-um all have a suppressive effect on the MHC-class II expression. Conclusions : Herb medicine generally show a suppressive effect on the suppression of the mesangial cell proliferation, fibronectin synthesis and MHC-class II expression.

• 대한한방내과학회지
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• 제27권1호
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• pp.138-148
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• 2006

Objectives: This study was designed to investigate the effects of Injinchunggan-tang(Yinchenqinggan-tang) on expression of angiogenic factors in HepG2 cells. Materials and Methods : The mRNA expression levels and protein secretion levels of angiogenic factors were measured using quantitative RT-PCR, Western blot and ELISA assay respectively in Injinchunggan-tang-treated and untreated HepG2 cells. Results : Injinchunggan-tang(Yinchenqinggan-tang) reduced mRNA expression levels and protein secretion levels of angiogenic factors, especially VEGF, bFGF and $TGF{\beta}1$ in HepG2 cells. Conclusion: Results indicate that Injinchunggan-tang (Yinchenqinggan-tang) inhibits expression of angiogenic factors in HepG2 cells. Further, results suggest that Injinchunggan-tang (Yinchenqinggan-tang) inhibits angiogenic effects in HCC.

• 동의신경정신과학회지
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• 제4권1호
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• pp.135-153
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• 1993

In order to observe clinical effects of Kwi-Bi-Tang(歸脾湯) and Sa-Mul-An-Shin-Tang(四物安神湯), I reached following conclusion through the physiochemical investigation the following results obtained. 1)In serum Lipid, only Total cholesterol is significantly decresed in medication group of Kwi-Bi-Tang(歸脾湯), but is not in the medicaton of Sa-Mul-An-Tang(四物安神湯). 2). The value of Cardiac enzyme is remarkably decreased in the medication group of Kwi-Bi-Tang(歸脾湯), Only AST is significantly decresed in the medication group of Sa-Mul-An-Tang(四物安神湯). 3) In determination of LDH isoenzyme, LDI is significantly decrease both in the medication group of Kwi-Bi-Tang(歸脾湯) and Sa-Mul-An-Shin-Tang(四物安神湯). Especially, LDI is remarkably decreased in the medication group of Kwi-Bi-Tang(歸脾湯). In view of the results so far achieved, we knew that Kwi-Bi-Tnag(歸脾湯) and Sa-Mul-An-Shin_Tang(四物安神湯) had improved ischemic condition of cardiac muscle, specially, Kwi-Bi-Tnag(歸脾湯) was significant compared to Sa-Mul-An-Shin-Tang(四物安神湯).

• 대한한의학방제학회지
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• 제19권1호
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• pp.103-111
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• 2011

Objectives : The purpose of this report was to provide the information of activity and safety of galgeun-tang by analyzing domestic/international papers and theses about galgeun-tang. Methods : Domestic/international papers and theses related to galgeun-tang were reviewed and analyzed. These papers were then classified by efficacy, or clinical trials. Results : The basic pharmacological experiment showed antipyretic, analgesic and anti-virus and anti-oxidant efficacy of galgeun-tang. In the case report of galgeun-tang, it showed therapeutic effect for patient with chronic rhinitis. But administration of galgeun-tang induced pruritic eruption in the two case report as a side effects of galgeun-tang. Conclutions : galgeun-tang showed tantipyretic, analgesic and anti-virus and anti-oxidant efficacy in the basic pharmacological experiment. Also, galgeun-tang showed therapeutic effect for patient with chronic rhinitis. But it was reported that galgeun-tang induced pruritic eruption in two case report, so physicians should be aware of the potential side effects.

• 대한한의학회지
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• 제21권1호
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• pp.3-10
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• 2000

We experimented with several prescriptions in order to find cure and prevention effects for obesity. We fed control group rats a high fat diet and adiministered normal saline for 8weeks. We fed experimental group rats a high fat diet and administered extract of four prescriptions for 8weeks. The results were as follows; 1. The serum total cholesterol level in Bikam-whan and Chekamhanghyuluiyiin-tang group showed a significant decrease in comparison with Control group. 2. The serum triglyceride level in Bikam-whan, Chekamhanghyuluiyiin-tang and Chekambangpungtongseong-san group showed a significant decrease in comparison with Control group. 3. The serum free fatty acid level in Chekamhanghyuluiyiin-tang and Chekambohyulansin-tang group showed a significant decrease in comparison with Control group. 4. The serum total lipid level in Bikam-whan, Chekamhanghyuluiyiin-tang and Chekambohyulansin-tang group showed a significant decrease in comparison with Control group. 5. The serum phospholipid level in Bikam-whan and Chekamhanghyuluiyiin-tang group showed a significant decrease in comparison with Control group. 6. The serum glucose level in Bikam-whan and Chekamhanghyuluiyiin-tang group showed a significant decrease in comparison with Control group. According to the above results, Bikam-whan and Chekamhanghyuluiyiin-tang has shown to be capable of curing and preventing obesty.

• 동의생리병리학회지
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• 제26권5호
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• pp.641-649
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• 2012

To investigate scientific evidence for the use of a Korean Medicine (KM), the papers regarding Sipjeondabo-tang (Shiquandabu-tang) frequently used in Korean medical clinics and hospitals were collected and analyzed. The papers were classified as being from domestic or international journals and by the year of publication. The mechanisms of immuno-activity Sipjeondabo-tang (Shiquandabu-tang) were researched. Among 41 papers, 4 were published as Korean papers whereas 7 were as Chinese papers and 40 were as Japanese papers. Sipjeondabo-tang (Shiquandabu-tang) regulated the productions of Th1 cytokines such as interleukin-2 (IL-2) and interferon-${\gamma}$ (IFN-${\gamma}$), and Th2 cytokines such as IL-4, IL-5, IL-6, IL-12 improving the function of immune organ including bone marrow, spleen, liver, thymus and peyer's patch. It also modulated the releases of immunoglobulin-G (IgG) and IgM. Thus, Sipjeondabo-tang (Shiquandabu-tang) balanced the Th1/Th2 immune responses as well as T/B cell. The regulation of immunoactivity could be speculated as an objective and scientific evidence of Sipjeondabo-tang (Shiquandabu-tang).

• 약학회지
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• 제38권5호
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• pp.555-561
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• 1994

In order to study the effect of some oriental prescriptions for polydipsia on experimental diabetes mellitus and cataract, Cheongsimyeonja-eum, Kamijowiseungki-tang and Kikukjihwang-tang were administered to streptozotocin(STZ)-induced diabetic and galactose-induced cataractous rats. The increased level of serum glucose and urea nitrogen owing to 572 were significantly decreased by administration of Cheongsimyeonja-eum, Kamijowiseungki-tang and Kikukjihwang-tang. The increased level of serum triglyceride and total cholesterol owing to STZ were significantly decreased by administration of Kamijowiseungki-tang and Kikukjihwang-tang. Reduction of body weight owing to STZ was significantly recovered in 7th day since administration of Cheongsimyeonja-eum and Kamijowiseungki-tang. Weight of liver was significantly recovered by administration of Cheongsimyeonja-eum and Kamijowiseungki-tang and weight of kidney was significantly recovered only by administration of Kikukjihwang-tang. The polydipsia of water and food were significantly decreased by administration of Cheongsimyeonja-eum, Kamijowiseungki-tang and Kikukjihwang-tang. Onset of cataract in experimental group owing to galactose was not significantly different from control group.

• 대한한의학방제학회지
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• 제27권2호
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• pp.179-187
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• 2019

Objective : The purpose of this paper is to analyze the difference between Baekho-tang(白虎湯) and Baekhogainsam-tang (白虎加人蔘湯). Methods : We compared the effect of Baekho-tang on atopic dermatitis and the effect of Baekhogainsam-tang on allergic contact dermatitis. We focused on the level of thirst. Results : After the Baekho-tang treatment, the SCORAD of atopic dermatitis patient decreased $63.8{\rightarrow}4.3$ and VAS of pruritus on atopic dermatitis patient were improved $8{\rightarrow}2$ respectively. Meanwhile, after the Baekhogainsam-tang treatment, the VAS of pruritus on allergic contact dermatitis patient was improved $7{\rightarrow}2$ and the thirst of the patient improved. Conclusions : There is a difference between Baekho-tang and Baekhogainsam-tang at the level of thirst. Baekhogainsam-tang continues to thirst hard enough to find water.