• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.91-103
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• 2021

To improve the fermentation quality of silage and reduce the nutrients loss of raw materials during the ensiling process, silage additives are widely used. The effect of additives on silage is also affected by the species of crop. Therefore, this study was designed to explore the effects of formic acid (FA) and lactic acid bacterial inoculant on the quality of main summer crop silage. The experiment was consisted on split-plot design with three replications. The experiment used the main summer forage crops of proso millet ("Geumsilchal"), silage corn ("Gwangpyeongok"), and a sorghum-sudangrass hybrid ("Turbo-gold"). Treatments included silage with Lactic acid bacterial Inoculant (Lactobacillus plantarum [LP], 1.0 × 106 CFU/g fresh matter), with FA (98%, 5 mL/kg), and a control (C, without additive). All silages were stored for 60 days after preparation. All additives significantly increased the crude protein content and in vitro dry matter digestibility (IVDMD) of the silages and also reduced the content of ammonia nitrogen (NH3-N) and pH. Corn had the highest content of IVDMD, total digestible nutrients and relative feed value among silages. Compared with the control, irrespective of whether FA or LP was added, the water soluble carbohydrate (WSC) of three crops was largely preserved and the WSC content in the proso millet treated with FA was the highest. The treatment of LP significantly increased the lactic acid content of the all silage, while the use of FA significantly increased the content of acetic acid (p < 0.05). The highest count of lactic acid bacteria (LAB) was detected in the LP treatment of corn. In all FA treatment groups, the total microorganism and mold numbers were significantly lower than those of the control and LP groups (p < 0.05). In conclusion, both additives improved the fermentation quality and nutritional composition of the main summer forage crops. The application of FA effectively inhibited the fermentation of the three crops, whereas LAB promoted fermentation. So, both FA and LP can improve the quality of various species of silage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.4
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• pp.360-365
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• 1991

This experiment was conducted to determine mechanized seeding ability of barley drill seeder on the seeding time, growth and yield of sorghum-sudangrass hybrid. The time required for machine drill seeding was 42 minutes per l0a which was one-thirteenth of the time required by the manual drill seeding, and percentage of seedling establishment by machine drill seeding was better than that of manual drill seeding. Leaf area index in seeding rate with 30 x 5cm and 40 x 5cm of machine drill seeding was higher than those of manual drill seeding, the leaf distribution was better and the total nonstructural carbohydrate content of stubble after cutting was short increased than that of manual drill seeding, Content of feed composition was not significantly different between manual and machine drill seeding. At seeding rate of 40 x 5cm of machine seeding. dry matter and TDN yield were increased to 29% and 30% respectiyely, than those of manual drill seeding.

• Journal of the Korea Organic Resources Recycling Association
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• v.31 no.2
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• pp.63-71
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• 2023

Increased nitrogen in the water system has become an important environmental problem around the world, as it causes eutrophication, algae bloom, and red tide, destroys the water system, and undermines water's self-purification. The most common form of nitrogen in the water system is ammonium ion (NH₄⁺), and the largest portion of ammonium ions comes from wastewater. NH₄⁺ is a major contributor to eutrophication, which calls for appropriate treatment and measures for ammonium removal. This study produced biochar by applying Sorghum × drummondii, a type of biomass with a great growth profile, analyzed the adsorption capacity of Sorghum × drummondii biochar produced from the changing carbonization temperature condition of 200 to 400℃ in the ammonium ion range of 10 to 100 ppm, and used the results to evaluate its potential as an adsorbent. Carbonization decomposed the chemical structure of Sorghum × drummondii and increased the content of carbon and fixed carbon in the biochar. The biochar's pH and electrical conductivity showed high adsorption potential for cations due to electrical conductivity as its pH and electrical conductivity increased along with higher carbonization temperature. Based on the results of an adsorption experiment, the biochar showed 54.5% and 17.4% in the maximum and minimum NH₄-N removal efficiency as the concentration of NH₄-N increased, and higher carbonization temperature facilitated the adsorption of pollutants due to the biochar's increased pores and specific surface area and subsequently improved NH₄-N removal efficiency. FT-IR analysis showed that the overall surface functional groups decreased due to high temperature from carbonization.

• Korean Journal of Organic Agriculture
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• v.13 no.2
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• pp.197-209
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• 2005

of Hangalku per plant sold for wild vegetables were 9.1g and 0.9g, and number of leaves was 10.8. Root fresh and dry weights of Hangalku per plant were 19.2g and 4.1g. Thirty five soil samples were collected from the native soils grown Cirsium japonicum DC., and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500${\mu}$m, 355~500${\mu}$m, 251~354${\mu}$m, 107~250${\mu}$m and 45~106${\mu}$m were 0.6, 2.1, 6.0, 55.3 and 126, etc. Total number of mycorrhizal spores per 30g fresh soil were 190. Root infection by vesicles, hyphae and arbuscules were 13%, 4% and 3%, respectively. As a result of identification, mass propagated mycorrhizal spores by the host plant of Sudangrass were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.

• Korean Journal of Organic Agriculture
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• v.13 no.2
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• pp.175-184
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• 2005

This study was conducted to investigate into the distribution of arbuscular mycorrhizal fungi (AMF) in the greenhouse soils grown strawberry plants in Damyang and Jangheung districts. Twenty three soil samples were collected from strawberry plants under greenhouse conditions, and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500${\mu}$m, 355~500${\mu}$m, 251~354${\mu}$m, 107~250${\mu}$m and $45{\sim}106{\mu}m$ were 0.3, 1.0, 4.2, 50.4 and 119, etc. Total number of spores per 30g fresh soil were l73.9. Root infection by vesicles and hyphae were 25% and 4%, respectively. Mycorrhizal root infection by arbuscules was not shown in strawberry roots. Isolated mycorrhizal spores were inoculated into the host plant of sudangrass to identify the genus of arbuscular mycorrhizal fungi, and propagated for 4 months. As a result of identification, mass propagated mycorrhizal spores were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.3
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• pp.219-224
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• 1996

Silage comkv, suwwn 19). sorghum $\times$ sudangrass(p. 988) and winter ryeNaton) were cultivated on imperfectly drained paddy field under two different draining methods, subsurface darinage by PVC pipes and open ditsched surface drainage. The crops were harvested at the stage of hard dough for corn and soft dough for wrghum and rye. The soil physical properties. soil colors. soil structure and soil wetness were improved in the subsurface drainage. Gravitational water table occured depth in 110 cm(dry season)~75cm(rain season). In soil profile description, yellowish brown with yellowish red mottles and well developed granular structure were found in the surface A horizon. The portion of solid phase in subsoils(B horizon) was reduced from 48.6%(undrained) to 43.7 %. A blocky structure with dark gray to gray were described in the open ditsched surface drainage. Severe wet depression of the crops was observed due to it's higher moisture contents, where the gravitational water occured depth in 25~37cm during the rainy season. The chemical properties of paddy soils were less affected by drainage methods. The concentration of available phosphate. organic matter and exchangeable K, Ca and Mg were decreased in the subsurface drained soils. The annual dry matter yields of com-rye cropping were 17.8 ton in the undrained, 21.6 ton in the open ditsch drainage and 35.9 ton/ha in the subsurface drainage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.3
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• pp.146-152
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• 1987

Sorghum cv. Pioneer 93 1, sorghum-sudangrass hybrid cv. Sioux and maize plant cv. Blizzard were assayed for toxic concentrations of nitrate-nitrogen ($NO_3$-N) and their relationship to morphological characteristics and environmental temperature in a field and phytotron trial. In the phytotron, sorghum and maize plants ranging from emergence to heading stage, were grown under different day/night temperatures of 30125, 25/20,28/18 and 1818 degree C. Nitrate-nitrogen in sorghum and maize plants was accumulated mainly in stems. Therefore nitrate concentration in the young plants was increased as development of stalks advanced and was highest at the stage of 3-4 leaves, when the plants had a leaf weight ratio 0.78-0.80 g/g plant weight. However, nitrate concentrations of the plant decreased as morphological development progressed, especially from the stage of growing point differentiation. Correlation coefficients showed a positive correlation of nitrate concentration with leaf weight ratio, leaf area ratio and specific leaf area, while plant height, dry matter percentage and absolute growth rate showed a negative association with TEX>$NO_3$-N ($P{\le}0.1$%). Cyanogenic glycosides, total nitrogen and crude protein were close associated with nitrate accumulation, and positively significant ($P{\le}0.1$%). High temperature over 30/25^{\circ}C.$ for 3 weeks increased N-uptake and dry matter accumulation, but reduced nitrate concentration. Under cold temperature below 18/8^{\circ}C.$ concentration of nitrate-N was increased in spite of its limited nitrogen uptake and plant growth.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.214-218
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• 2014

This study was conducted to assess the feasibility of near-infrared reflectance spectroscopy (NIRS) as a rapid and reliable method for the estimation of crude protein (CP) fractions in forage legume mixtures (sudangrass and pea mixture, and kidney bean and potato mixture). A total of 178 samples were collected and their spectral reflectance obtained in the range of 400~2,500 nm. Of these, 50 samples were selected for calibration and validation, and 35 samples were used for calibration of the data set, and the modified partial least square regression (MPLSR) analysis was performed. The correlation coefficient ($r^2$) and the standard error of cross-validation (SECV) of the calibration models in the CP fractions, A, B1, B2, B3, and C, were 0.94 (1.05), 0.92 (0.74), 0.96 (0.95), 0.91 (0.42), and 0.83 (0.38), respectively. Fifteen samples were used for equation validation, and the $r^2$ and the standard error of prediction (SEP) were 0.87 (1.45), 0.91 (0.49), 0.94 (1.13), 0.36 (0.96), and 0.74 (0.67), respectively. This study showed that NIRS could be an effective tool for the rapid and precise estimation of CP fractions in forage legume mixtures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.105-112
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• 1996

In order to select the forage cowpea of high dry matter and protein yield, growth characteristics and yield performance of forage cowpea were investigated. The results obtained are as follows: Arn0r.g the five varieties tested, lT820-889 and lT83S-852 were top grass type, but IT830422 and lT84E-124 were short grass type. In stem diameter of soilage cowpea, IT83S-852 was the highest as 9.5mrn, but Swwon was the lowest as 7.8mm. In stem hardiness, lT84E-124 was the highest as 1.9kg/$cm^2$, but Seowon was the lowest as 0.8kg/$cm^2$. The palatability was high in the order of lT83S-852 > IT820-889 > Swwon, while IT820489 and IT 83S- 852 were lower than other varieties in 1990 and 1991 year. But palatability of cowpea was wholly lower than other soilage(Sudangrass hybrid and Soybean). In total dry matter yield and protein yield, lT820-889 and IT83S-852 were higher but IT830422 and lT84E- 124 were lower than other varieties. In conclusion, among the five varieties used, IT820-889 and lT83S-852 were higher as forage cowpea.

• Horticultural Science & Technology
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• v.18 no.6
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• pp.802-807
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• 2000

This study was conducted to propagate the arbuscular mycorrhizal fungi in vitro using the hairy root of carrot transformed by Agrobacterium rhizogenes with Ri t-DNA. Mycorrhizal spores and roots in sudangrass plants were wet-sieved, surface-sterilized and inoculated onto the hairy root of carrot on the Modified Strullu & Romand (MSR) medium. The mycorrhizal spores of Glomus sp. propagated in vitro for 12 weeks was about $50{\mu}m$, and the shapes of spores were round or elliptic. Spores were formed mainly at the middle of the hyphae. Number of mycorrhizal spores propagated using dual culture of the transformed carrot roots and the mycorrhizal inoculum for 12 weeks were about 1,200 per plates.