• 대한간호학회지
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• 제51권5호
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• pp.630-642
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• 2021

Purpose: This study aimed to identify the factors influencing the interaction between mothers and preschool-age children and construct a structural equation model based on the factors identified. Methods: The data were collected from the mothers of children aged three to six years enrolled in kindergartens in Seoul, Gyeonggi, Chungbuk, and Jeonbuk. A total of 328 questionnaires were used for the analysis. Analysis was performed using SPSS 26.0 and AMOS 26.0 programs. Results: The child's self-regulation (β = .42, p < .001) had the most significant influence on the interaction between mothers and preschool children, followed by the mother's parenting stress (β = - .23, p = .008), social support (β = .17, p < .001), and the child's active temperament (β = .15, p < .001). The child's gender, emotional temperament, social temperament, and mother's education level had indirect influences. Conclusion: These results suggest that interventions considering the child's temperament, mother's parenting stress, and social support are required to promote the interaction between preschool-age children and mothers.

• Applied Microscopy
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• 제51권
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• pp.13.1-13.7
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• 2021

The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has arisen as a global pandemic affecting the respiratory system showing acute respiratory distress syndrome (ARDS). However, there is no targeted therapeutic agent yet and due to the growing cases of infections and the rising death tolls, discovery of the possible drug is the need of the hour. In general, the study for discovering therapeutic agent for SARS-CoV-2 is largely focused on large-scale screening with fragment-based drug discovery (FBDD). With the recent advancement in cryo-electron microscopy (Cryo-EM), it has become one of the widely used tools in structural biology. It is effective in investigating the structure of numerous proteins in high-resolution and also had an intense influence on drug discovery, determining the binding reaction and regulation of known drugs as well as leading the design and development of new drug candidates. Here, we review the application of cryo-EM in a structure-based drug design (SBDD) and in silico screening of the recently acquired FBDD in SARS-CoV-2. Such insights will help deliver better understanding in the procurement of the effective remedial solution for this pandemic.

• 유통과학연구
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• 제11권4호
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• pp.89-97
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• 2013

Purpose - The purpose of this study is to systematically review the background of the Korean distribution promotion policy and distribution adjustment policies along with related regulations and policies. Research design, data, and methodology - Domestic distribution policy and relevant laws were examined through a review of existing research literature. The results of the development process of the domestic distribution policy, promotion policies, and adjustment policies are summarized below. Results - The results are summarized as follows. First, the purpose of the development of the domestic distribution promotion policy was to strengthen the competitiveness of the small and medium business industry through structural advancement of the small and medium industry. By expanding the managerial base for the small and medium industry, a new balance could be created in the national economy. There was a requirement for an early assistance policy for small and medium businesses as a base of these businesses in the distribution industry developed from their original model of catering to a traditional market of retail shops. Since 1996, there was a need for this early assistance policy due to the expansion and rapid growth of large scale stores causing a change in the consumption pattern for distribution markets and the decline of large enterprises. Second, the government supports small and medium business distribution through distribution promotion policies by supporting an organization promoting small business and supporting innovation in the distribution system. Third, in 1961 a business mediation system was established to protect small and medium industries. The Small and Medium Business Administration advises conglomerates to postpone acquisitions, restrain expansion of the business, or to reduce business scale if small businesses undergo an adverse effect such as decreasing demand because large companies are expanding into their areas. Fourth, the Distribution Adjustment Policy managed large-scale store regulation as follows: ① limitation on construction by urban planning ordinance, ② limitation on location based on traffic impact assessments, ③ regulation based on business guidelines by chiefs of autonomous bodies, ④ regulation on mandatory holidays and limitation of business hours. This large-scale store regulation is a policy introduced by authority to increase competitiveness of small and medium business distribution by the government. Conclusions - As discussed in this study, the distribution promotion policy and distribution adjustment policy are government distribution policies focused on the protection of the small and medium distribution businesses. This study is timely, since it was planned when the strengthening of the revisions of the Distribution Industry Development Act, aimed to protect small and medium retailers and merchants, was under discussion. The significance of this study is that it offers insights for the development of new policies in the future and an opportunity to consider the background of the distribution policy by the government.

• 한국언론정보학보
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• 제55권
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• pp.50-73
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• 2011

현재 국내에서 시행되고 있는 '제한적 본인확인제(인터넷실명제)'는 인터넷의 본질적 속성인 익명성을 통제하여 이로 인한 부정적 측면을 최소화하려는 취지에서 도입되었다. 법제도의 성패와 영향력은 이의 적용을 받는 사람들의 해당 법제도에 대한 인식과 대응에 따라 달라진다고 볼 때, 인터넷처럼 법의 적용범위가 명확하지 않으며 다른 선택대안이 존재하는 대상일 경우 이용자의 태도와 선택은 더 큰 의미를 지닌다. 이 연구는 인터넷실명제에 대한 태도와 이 제도가 가져온 환경변화에 대한 인식에 영향을 미치는 요인들을 살펴봄으로써 이 제도에 대해 서로 상반된 입장을 보이는 사람들의 성향에 어떤 차이가 있는가를 경험적으로 검토하였다. 또한 온라인공론장의 참여도, 제도에 대한 태도와 환경인식, 여러 대안들에 대한 선택 의향 등 관련 변인들간의 관계에 대한 경험적 분석을 시도하였다. 분석 결과, 나이가 어리고 정치적으로 진보적이며 정치적 관심도가 높을수록, 또한 프라이버시 보호에 대한 인식이 강하고, 온라인공론장에의 참여도가 높을수록 인터넷실명제 유지에 반대하는 것으로 나타났다. 나아가 온라인공론장 참여도는 인터넷실명제에 대한 직간접적인 태도에 영향을 미치고 이러한 태도는 다시 여러 대안에 대한 선택의도에 영향을 미친다는 가정을 검증하였다. 구조방정식모형(SEM) 분석결과, 온라인공론장 참여도는 인터넷실명제 및 관련 환경 변화에 대한 인식과 유의미한 관계에 있음을 확인할 수 있었고 이는 다시 자기검열, 사이버망명, 서비스망명과 같은 우회로 선택 의사와 유의미하게 연결되었다. 이러한 연구결과는 인터넷이용자 특히 온라인공론장 참여가 활발한 이용자층의 인식과 선택에 따라 인터넷실명제의 실효성이 크게 달라질 수 있음을 보여준다.

• Molecules and Cells
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• 제44권1호
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• pp.26-37
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• 2021

Human papillomaviruses (HPVs) cause cellular hyperproliferation-associated abnormalities including cervical cancer. The HPV genome encodes two major viral oncoproteins, E6 and E7, which recruit various host proteins by direct interaction for proteasomal degradation. Recently, we reported the structure of HPV18 E7 conserved region 3 (CR3) bound to the protein tyrosine phosphatase (PTP) domain of PTPN14, a well-defined tumor suppressor, and found that this intermolecular interaction plays a key role in E7-driven transformation and tumorigenesis. In this study, we carried out a molecular analysis of the interaction between CR3 of HPV18 E7 and the PTP domain of PTPN21, a PTP protein that shares high sequence homology with PTPN14 but is putatively oncogenic rather than tumor-suppressive. Through the combined use of biochemical tools, we verified that HPV18 E7 and PTPN21 form a 2:2 complex, with a dissociation constant of 5 nM and a nearly identical binding manner with the HPV18 E7 and PTPN14 complex. Nevertheless, despite the structural similarities, the biological consequences of the E7 interaction were found to differ between the two PTP proteins. Unlike PTPN14, PTPN21 did not appear to be subjected to proteasomal degradation in HPV18-positive HeLa cervical cancer cells. Moreover, knockdown of PTPN21 led to retardation of the migration/invasion of HeLa cells and HPV18 E7-expressing HaCaT keratinocytes, which reflects its protumor activity. In conclusion, the associations of the viral oncoprotein E7 with PTPN14 and PTPN21 are similar at the molecular level but play different physiological roles.

• 한국전산구조공학회논문집
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• 제28권2호
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• pp.169-176
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• 2015

교량의 교각 설계시에는 설계기준에 의거하여 차량의 충돌에 대비한 적절한 방호시설 등이 고려되고 있으나, 지하차도의 기둥 설계시에는 차량의 충돌과 관련된 규정이 없다. 또한 지하차도의 기둥은 상대적으로 폭이나 두께가 작아서 차량의 충돌에 의하여 큰 손상이 발생할 수 있다. 본 논문에서는 지하차도 기둥에 대한 충돌해석을 통하여 차량의 충돌에 의한 구조물의 손상을 평가하였다. 충돌해석에서는 지하차도 기둥의 물성과 형태 그리고 차량의 속도와 종류 등이 매개변수로 고려되었다. 수치해석 결과 지하차도 기둥이 심하게 손상되는 경우가 있었으며 따라서 지하차도의 기둥 설계시에는 차량 충돌에 대한 적절한 고려가 필요하다.

• 복합신소재구조학회 논문집
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• 제4권2호
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• pp.15-24
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• 2013

This project is mainly related to evaluation of total energy consumption of low energy house, the exterior envelope of which was wholly composed of structural insulated panels(SIP). The U-value of applied SIP was in the range of 0.189 to $0.269W/m^2{\cdot}K$ and the U-value of pair glass from 0.78 to $1.298W/m^2{\cdot}K$ was applied for window dependent to its function respectively. For comparison of total energy performance, the energy simulation for pilot house was performed to compare with the control house having insulation criteria of Korean building regulation in 2009. Based on simulation of dynamic energy performance, the pilot house saved 48.3% of annual energy consumption while the control house in 2009 consumed as 85.7GJ/y. In case of heating, the result showed that the energy saving ratio amounted to 76.7%. For $CO_2$ emission, the pilot house diminished approximately 35.4% from $6,208.4kgCO_2$ to $4,009.2kgCO_2$. In payback period to early investment, it was analyzed the pilot house took 7.8 years, when the low energy house built by other insulation method with same thermal perfusion took 11.5 years. From this result, it is considered that the SIP is more effective, economic to Green Home application.

• 한국산업융합학회 논문집
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• 제26권1호
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• pp.175-181
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• 2023

In offshore plants, various equipments including cranes and davits are used for safety management. Hydraulic cylinder type actuators are mainly used for luffing operations such as cranes and davits. However, in the case of a cylinder using hydraulic pressure, a separate power pack is required to supply hydraulic pressure. When used for a long time, maintenance costs such as sticking of hydraulic valves, contamination of hydraulic oil and deterioration of hydraulic hoses occur. In addition, a lot of hydraulic oil is used in the handling of cranes and davits, which causes marine pollution due to management problems. As a result, as interest in marine pollution prevention has increased recently, interest in actuators that do not use hydraulic pressure is also increasing. Therefore, in this study, we intend to develop a davit with an electric cylinder type actuator that uses electricity rather than hydraulic pressure by the SOLAS regulation. In other words, the conceptual design of the davit driven through the linear motion of the ball screw using electricity is performed, and the structural safety of the drive is also reviewed so that it can be utilized in the industrial field.

• Ecology and Resilient Infrastructure
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• 제3권3호
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• pp.182-188
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• 2016

본 연구는 도시 홍수문제의 구조적 해결방안을 도출하기 위하여 내구성-비용지수 (robustness cost index, RCI)를 수질오염 문제 사례에 재해석 및 적용하였다. 처리기준 산정방식이 다른 하수 처리수 내 내생호르몬 오염과 취수원 원생동물 번성을 대표 사례로 선정하여 기존시설과 대안시설 (구조적 대안)의 내구성 지수 (RI) 값이 1 이상인 경우를 내구성을 확보한 대안으로 판정하고 내구성 지수와 비용지수 (CI)를 결합한 RCI 값을 산정하였다. 하수 처리수 내 내생호르몬 오염은 인체로부터 기인하는 $17{\beta}$-estradiol 을 대상 오염물질로 하여 현재 하수처리시설과 대안시설 (고도처리공정 증축)을 비교하였고, 고도처리공정을 증축한 사례에서 RI뿐만 아니라 RCI 값이 큰 결과가 나타났다. 취수원 원생동물 번성의 경우 크립토스포리디움을 처리하기 위한 소독방안으로 자외선 소독과 오존 소독을 대상으로 RCI를 산정하였고 오존 소독시설이 RCI값이 더 높은 것을 확인하였다. 처리기준이 다른 수질오염사례의 RCI 산정과정과 도출한 값을 바탕으로 재해 발생 시 피해 복구와 피해 방지를 위한 구조적 대안 수립과정의 방향을 제시하고 그 구축전략을 제안하였다.

• Toxicological Research
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• 제14권2호
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• pp.211-216
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• 1998

In order to evaluate the mutagenic potential of SDK(skin decontamination kit) produced by Agency for Defense Development(ADD), were performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells according to the established regulation of Korean Food and Drug Administration. In the reverse mutation test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 did not in-crease the number of revertant at any of the concentration tested in this study. SDK did not increase the number of cells having structural or numerical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase in the occurrence oj micro nucleated polychromatic erythrocytes were observed in ICR male mice intraperitoneally administered with SDK. These results indicate that SDK has no mutagenic effects under these experimental conditions.