• Title/Summary/Keyword: strain-dependency

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Temperature-Dependency Urease Activity in Vibrio parahaemolyticus is Related to Transcriptional Activator UreR

  • Park, Kwon-Sam;Lee, Soo-Jae;Chung, Yong-Hyun;Iida, Tetsuya;Honda, Takeshi
    • Journal of Microbiology and Biotechnology
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    • v.19 no.11
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    • pp.1456-1463
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    • 2009
  • Vibrio parahaemolyticus possessing urease-positive property is relatively rare, but such strains consistently exhibit the TDH-related hemolysin (TRH) gene. In this study, we examined the effects of incubation temperature on urease activity expression, using the TH3996 and AQ4673 strains where the enzyme activity is known to be temperature-dependent and -independent, respectively. In the TH3996 strain, $\beta$-galactosidase activity was 4.4-fold lower after $30^{\circ}C$ cultivation than after $37^{\circ}C$ in a ureR-lacZ fusion strain, but temperature dependency was not found in ureD- or nikA-lacZ fusion strains. However, ureR-, ureD-, and nikA-lacZ fusions of the AQ4673 strain was not influenced by incubation temperature. We compared the promoter sequences of ureR between the above two strains. Intriguingly, we detected mismatches of two nucleotides between the two strains located at positions -66 and -108 upstream of the methionine initiation codon for UreR. Additionally, urease activity was not affected by culture temperature at either $30^{\circ}C$ or $37^{\circ}C$ by allelic introduction of the AQ4673 ureR gene into the TH3996 ureR deletion mutant. Taken together, our study demonstrates that the transcriptional factor UreR is involved in the temperature dependency of urease activity, and two nucleotides within the ureR promoter region are of particular importance for the urease activity dependency of V. parahaemolyticus.

Finite Strain and Nonlinear Consolidation Analysis Considering the Effect of Strain Rate Dependency on Clay (점토의 변형률 속도 의존성을 고려한 비선형 유한변형 압밀해석)

  • Lee, Bongjik;Lee, Heunggil;Kwon, Youngcheul
    • Journal of the Korean GEO-environmental Society
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    • v.9 no.6
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    • pp.53-60
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    • 2008
  • In recent years, finite strain consolidation theories including a mechanical nonlinearity and a reasonable coordinate system have been proposed and used in educations and practical consolidation problems. However, despite their reasonable ability to predict the consolidation behavior, their failure in the field can be attributed to the complexity of estimating and selecting proper parameters for simulating the consolidation phenomenon. In this study, therefore, the application of a piecewise-linear method was proposed to solve such problems including the assumption of the uniqueness in compressibility. Especially, the concept of reference curve was introduced to define the effect of strain rate dependency of clay. The applicability of the methodology is verified by several tests. It was found that the proposed method is applicable in restrictive ranges of study carried out in the laboratory. Finally it is expected that the verification in field consolidation problem has to be carried out through future study.

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Modeling of Size-Dependent Strengthening in Particle-Reinforced Aluminum Composites with Strain Gradient Plasticity (변형률 구배 소성을 고려한 입자 강화 알루미늄 복합재의 크기 종속 강화 모델링)

  • Suh, Yeong-Sung;Park, Moon-Shik;Song, Seung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.35 no.7
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    • pp.745-751
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    • 2011
  • This study proposes finite element modeling of dislocation punching at cooling after consolidation in order to calculate the strength of particle-reinforced aluminum composites. The Taylor dislocation model combined with strain gradient plasticity around the reinforced particle is adopted to take into account the size-dependency of different volume fractions of the particle. The strain gradients were obtained from the equivalent plastic strain calculated during the cooling of the spherical unit cell, when the dislocation punching due to CTE (Coefficient of Thermal Expansion) mismatch is activated. The enhanced yield stress was observed by including the strain gradients, in an average sense, over the punched zone. The tensile strength of the SiCp/Al 356-T6 composite was predicted through the finite element analysis of an axisymmetric unit cell for various sizes and volume fractions of the particle. The predicted strengths were found to be in good agreement with the experimental data. Further, the particle-size dependency was clearly established.

Factors Influencing Adult Media Engagement of Adolescents from Micro-systemic Perspective: Mediation Analysis of Cell-Phone Dependency (미시체계적관점에서의 청소년 성인매체 몰입에 미치는 영향요인: 휴대폰 의존의 매개효과 검정)

  • Shim, Hyun-Jin;Lee, Hee-Myung;Rhee, Hyun-Sill
    • Journal of the Korean Society of School Health
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    • v.29 no.2
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    • pp.63-70
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    • 2016
  • Purpose: The purpose of this study was to find the factors influencing adolescents' adult media engagement. The study is based on Agnew's General Strain Theory (GST) and a micro-systemic perspective. Methods: The study examined 479 middle school freshmen who participated in the fourth wave of the Korean Children & Youth Panel Survey (KCYPS) conducted by National Youth Policy Institute. Data were analyzed through descriptive statistics, factor analysis, and reliability analysis using SPSS 22.0 Ver. Also, hypothesis testing was performed via covariance-based structure equation modeling (CB-SEM) using Amos 21.0 Ver. Results: Strain factors had positive effects on adolescent depression, and depression had a positive effect on adult media engagement. Also, cell-phone dependency had a positive mediation effect between depression and adult media engagement. Conclusion: The study's result identified the factors affecting adult media engagement. Therefore, we suggest intervention programs considering adolescents' strains, depression and cell-phone dependency to improve their health.

A Tensile Criterion to Minimize FE Mesh-Dependency in Concrete Beam under Blast Loading (폭발하중을 받는 콘크리트 보의 요소의존성 최소화 인장기준식)

  • Kwak, Hyo-Gyoung;Gang, HanGul
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.30 no.2
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    • pp.137-143
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    • 2017
  • A tensile failure criterion that can minimize the mesh-dependency of simulation results on the basis of the fracture energy concept is introduced, and conventional plasticity based damage models for concrete such as CSC model and HJC model, which are generally used for the blast analyses of concrete structures, are compared with orthotropic model in blast test to verify the proposed criterion. The numerical prediction of the time-displacement relations in mid span of the beam during blast loading are compared with experimental results. Analytical results show that the numerical error is substantially reduced and the accuracy of numerical results is improved by applying a unique failure strain value determined according to the proposed criterion.

An Criterion to Minimize FE Mesh-Dependency in Concrete Plate under Impact Loading (충격하중을 받는 판형콘크리트 구조물의 요소의존성 최소화 기준식)

  • Kwak, Hyo-Gyoung;Gang, Han-Gul;Park, Lee-Ju
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.3
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    • pp.147-154
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    • 2014
  • In the context of an increasing need for safety in concrete structures under blast and impact loading condition, the behavior of concrete under high strain rate condition has been an important issue. Since concrete subjected to impact loading associated with high strain rate shows quite different material behavior from that in the static state, several material models are proposed and used to describe the high strain rate behavior under blast and impact loading. In the process of modelling high strain rate conditions with these material models, mesh dependency in the used finite element(FE) is the key problem because simulation results under high strain-rate condition are quite sensitive to applied FE mesh size. This paper introduces an criterion which can minimize the mesh-dependency of simulation results on the basis of the fracture energy concept, and HJC(Holmquist Johnson Cook) model is examined to trace sensitivity to the used FE mesh size. To coincide with the purpose of the perforation simulation with a concrete plate under a projectile(bullet), the residual velocities of projectile after perforation are compared. The analytical results show that the variation of residual velocity with the used FE mesh size is quite reduced and accuracy of simulation results are improved by applying a unique failure strain value determined according to the proposed criterion.

Tensile Strain Characteristics of Critical Current in YBCO Coated Conductors (YBCO CC테이프 임계전류의 인장변형률 특성)

  • Shin, Hyung-Seop;Kim, Ki-Hyun;Oh, Sang-Soo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.07a
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    • pp.274-275
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    • 2005
  • The tensile strain dependency of critical current in YBCO coated conductors was examined at 77K and in the self magnetic field. A commercially available YBCO sample with Cu stabilizer layer was supplied. There existed a peak in the relation between the Ie and tensile strain, and the reversible variation of $I_c$ with applied tensile strain was found. In the neutral axis Ni alloy RABiTS-$Y_2O_3$/YSZ/$CeO_2$ buffered YBCO tape, the $I_c$ recovered reversibly until the applied strain reached to about 0.5%, representing that a significant residual compressive strain induced during cooling to 77 K influenced the axial strain tolerance of YBCO conductors. To investigate the strain and stress influence on the $I_c$, the stress-strain characteristics of YBCO conductors measured at 77 K were discussed.

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An Experimental Study on the Dynamic Increase Factor and Strain Rate Dependency of the Tensile Strength of Rock Materials (암석재료 인장강도의 동적 증가계수 및 변형률 속도 의존성에 대한 실험적 연구)

  • Oh, Se-Wook;Choi, Byung-Hee;Min, Gyeong-Jo;Jung, Yong-Bok;Cho, Sang-Ho
    • Explosives and Blasting
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    • v.39 no.1
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    • pp.10-21
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    • 2021
  • Brittle materials such as rocks and concretes exhibit large strain-rate dependency under dynamic loading conditions. This means that the mechanical properties of such materials can significantly be varied according to load velocity. Thus, the strain-rate dependency is recognized as one of the most important considerations in solving problems of blast engineering or rock dynamics. Unfortunately, however, studies for characterizing the dynamic properties of domestic rocks and other brittle materials are still insufficient in the country. In this study, dynamic tensile tests were conducted using the Hopkinson pressure bar apparatus to characterize the dynamic properties of Geochang granite and high-strength concrete specimens. The dynamic Brazilian disc test, which is suggested by ISRM, and the spalling method were applied. In general, the latter is believed to have some advantages in experiments under high-strain rate deformation. It was found from the tests that there were no significant difference between the dynamic tensile strengths obtained from the two different test methods for the two materials given. However, this was not the expected result before the tests. Actually, authors expected that there be some differences between them. Hence, it is thought that further investigations are needed to clarify this results.

A Study on Strain Rate Sensitivity by Unified Viscoplasticity (점소성 이론에 의한 변형률 속도 민감도에 대한 연구)

  • 호괄수
    • Transactions of Materials Processing
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    • v.13 no.7
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    • pp.600-607
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    • 2004
  • This paper addresses a viscoplastic constitutive model that allows a consistent way of modeling positive and negative rate sensitivities of flow stress concerned with dynamic strain aging. Based on the concept of continuum mechanics, a phenomenological constitutive model includes the use of a yield surface within the framework of unified viscoplasticity theory. To model negative rate sensitivity, rate-dependent back stress is introduced and flow stress in fully developed inelastic deformation regime is thus decomposed into the plastic contribution of rate independency and the viscous one of rate dependency.

Development & Verification of Frequency-Strain Dependence Curve (주파수-변형률 곡선의 개발 및 검증)

  • Jeong, Chang-Gyun;Kwak, Dong-Yeop;Park, Du-Hee
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.03a
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    • pp.146-153
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    • 2009
  • One dimensional site response analysis is widely used in prediction of the ground motion that is induced by earthquake. Equivalent linear analysis is the most widely used method due to its simplicity and ease of use. However, the equivalent linear method has been known to be unreliable since it approximates the nonlinear soil behavior within the linear framework. To consider the nonlinearity of the ground at frequency domain, frequency dependent algorithms that can simulate shear strain - frequency dependency have been proposed. In this study, the results of the modified equivalent linear analysis are compared to evaluate the degree of improvement and the applicability of the modified algorithms. Results show the novel smoothed curve that is proposed by this study indicates the most stable prediction and can enhance the accuracy of the prediction.

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