• Journal of Biomedical Engineering Research
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• v.34 no.1
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• pp.14-23
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• 2013

In this study, biomechanical stability of the newly developed revision total knee arthroplasty (rTKA) was evaluated through strain and stress distribution analysis within the implanted proximal tibia using a three-dimensional finite element (FE) analysis. 2000N of compressive load (about 3 times body weight) was applied to the condyle surface on spacer, sharing by the medial (60%) and lateral (40%) condyles simulating a stance phase before toe-off. The results showed that PVMS within the revision total knee arthroplasty and the proximal tibia were less than yield strength considering safe factor 4.0 (rTKA: less than 10%, Cortical bone: less than 70%, Cancellous bone: less than 70%). The materials composed of them and the strain and stress distributions within the proximal tibia were generally well matched with those of a traditional revision total knee arthoplasty (Scorpio TS revision system, Stryker Corp., Michigan, USA) without the critical damage strain and stress, which may reduce the capacity for bone remodeling, leading to bone degeneration. This study may be useful to design parameter improvement of the revision total knee arthoplasty in biomechanical stability point of view beyond structural stability of revision total knee arthoplasty itself.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.184-189
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• 2012

We sought to breed an industrially useful yeast strain, specifically an ethanol-tolerant yeast strain that would be optimal for ethanol production, using a novel breeding method, called genome reconstruction, based on chromosome splitting technology. To induce genome reconstruction, Saccharomyces cerevisiae strain SH6310, which contains 31 chromosomes including 12 artificial mini-chromosomes, was continuously cultivated in YPD medium containing 6% to 10% ethanol for 33 days. The 12 mini-chromosomes can be randomly or specifically lost because they do not contain any genes that are essential under high-level ethanol conditions. The strains selected by inducing genome reconstruction grew about ten times more than SH6310 in 8% ethanol. To determine the effect of mini-chromosome loss on the ethanol tolerance phenotype, PCR and Southern hybridization were performed to detect the remaining mini-chromosomes. These analyses revealed the loss of mini-chromosomes no. 11 and no. 12. Mini-chromosome no. 11 contains ten genes (YKL225W, PAU16, YKL223W, YKL222C, MCH2, FRE2, COS9, SRY1, JEN1, URA1) and no. 12 contains fifteen genes (YHL050C, YKL050W-A, YHL049C, YHL048C-A, COS8, YHLComega1, ARN2, YHL046W-A, PAU13, YHL045W, YHL044W, ECM34, YHL042W, YHL041W, ARN1). We assumed that the loss of these genes resulted in the ethanol-tolerant phenotype and expect that this genome reconstruction method will be a feasible new alternative for strain improvement.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.2
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• pp.158-165
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• 2019

The purpose of this study is to investigate analytically the flexural behavior of beam reinforced by an alkali-activated slag-based fiber-reinforced composite. The materials and mixture proportion were selected to manufacture an alkali-activated slag-based fiber-reinforced composite with high tensile strain capacity over 7% and compressive strength and tension tests were performed. The composite showed a compressive strength of 32.7MPa, a tensile strength of 8.43MPa, and a tensile strain capacity of 7.52%. In order to analyze the flexural behavior of beams reinforced by ultra-high-ductile composite, nonlinear sectional analysis was peformed for four types of beams. Analysis showed that the flexural strength of beam reinforced partially by ultra-high-ductile composite increased by 8.0%, and the flexural strength of beam reinforced fully by ultra-high-ductile composite increased by 24.7%. It was found that the main reason of low improvement in flexural strength is the low tensile strain at the bottom of beam. The tensile strain at bottom corresponding to the flexural strength was 1.38% which was 18.4% of tensile strain capacity of the composite.

• The Journal of the Acoustical Society of Korea
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• v.29 no.8
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• pp.504-508
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• 2010

In conventional diagnostic ultrasound strain imaging, when displaying strain image on a monitor, human visual characteristics are utilized such that hard regions are displayed as dark and soft regions are displayed as bright. Thus, hard regions representing tumor or cancer are displayed as dark, decreasing the contrast inside the lesion. Because the lesion area is stiff and thus displayed as dark, a method of inverting the image brightness and thereby increasing the contrast in the lesion for better diagnostic purposes is proposed wherein a postcompression signal is extended in the time domain by a factor corresponding to the reciprocal of the amount of the applied compression using a technique termed globally uniform stretching. Experiments were carried out to verify the proposed method on an ultrasound elasticity phantom with radio-frequency data acquired from a diagnostic ultrasound clinical scanner. It is found that the new method improves the contrast-to-noise ratio by a factor of up to about 1.8 compared to a conventional strain imaging method that employs a reversed gray color map without globally uniform stretching.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.226-228
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• 2007

During asymmetrical cold rolling in AA 5052 sheet a reduction per a rolling pass was varied to investigate the effect of the ratio of the contact length between the roll and sample ($l_c$) to the sheet thickness (d) on the formation of shear textures. In order to intensify the shear deformation during asymmetrical rolling, AA 5052 sheet was asymmetrically cold rolled without lubrication by using different roll velocities of upper and lower rolls. Asymmetrical rolling with $l_c$/d=1.8 led to the formation of texture gradients throughout the sheet thickness in which the outer thickness layers depicted shear textures and the center thickness layers displayed a rolling texture. Asymmetrical rolling with $l_c$/d=3.1 gave rise to the formation of shear textures in the whole through-thickness layer. The strain states associated with asymmetrical rolling were investigated by the finite element method (FEM) simulation. FEM results indicated that the evolution of deformation texture in a thickness layer is strongly governed by integrated values of strain rates and along the streamline in the roll gap.

• Korean Journal of Materials Research
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• v.15 no.4
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• pp.240-245
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• 2005

An oxygen free copper was severely deformed by accumulative roll-bonding (ARB) process for improvement of its mechanical properties. Two copper sheets 1 m thick, 30 mm wide and 300 m long are first degreased and wire-brushed for sound bonding. The sheets are then stacked to each other, and roll-bonded by about $50\%$ reduction rolling without lubrication at ambient temperature. The bonded sheet is then cut to the two pieces of same dimensions and the same procedure was repeated to the sheets up to eight cycles $(\varepsilon-6.4)$. TEM observation revealed that ultrafine grains were developed after the third cycle, and their size was slightly increased at higher cycles. Tensile strength of the copper increased with the strain at low strain levels, but it hardly increased from 3 cycles $(\varepsilon>2.4)$ due to occurrence of dynamic recovery, even if the imposed strain increased.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.8
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• pp.2650-2669
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• 1996

A new low Reynolds number nonlinear second moment turbulence closure was introduced to analyze a square sectioned 180.deg. bend flow. Inclusion of nonlinear return to isotropy term and cubic mean pressure strain term has brought out a marked improvement in the level of agreement with measured velocity profiles. Optimization of present closure was performed by comparison of computed velocity profiles with the experimental ones with variation of nonlinear return to isotropy term and quadratic and cubic pressure-strain model. Progressive vortex breakdown due to the interaction of primary and secondary flows was well captured by using the optimized second moment turbulence closure.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.1
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• pp.58-65
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• 2010

For the improvement of fuel consumption, the study on the use of lightweight material or thinner sheet have been carried out in automotive industry. With the need for the use of thinner sheet, the dent resistance became one of the major concern in th design of exterior panels in automotive industry. Many studies have been carried out for the dent resistance by experiment or quasi-static numerical simulation. In this study, the dent formation behavior is investigated by dynamic finite element analysis using ABAQUS. Dent formation may be affected by many factors such as sheet thickness, material properties, pre-strain, and sheet curvature. The effect of these factors on dent resistance is investigated. From the analysis following three conclusions are derived. First, dent resistance become hard as the sheet curvature radius increases. Second, dynamic dent resistance is mainly affected by bending stress rather than tensile stress. Third, the pre-strain itself do not give any guidance for dynamic dent resistance and dynamic dent resistance have to be decided considering the strain hardening and thickness reduction together. The results are considered to be reliable and useful to improve the dent damage of automotive panels.

• Mycobiology
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• v.39 no.1
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• pp.20-25
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• 2011

Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with $Co^{60}$ ${\gamma}$-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or $NH_4Cl$, 1% (w/w) rice starch, 2.5 mM $MgCl_2$, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at $35^{\circ}C$ for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.

• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.718-726
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• 2010

A selected fungal strain, for production of the raw-starchdigesting enzyme by solid-state fermentation, was improved by two repeated sequential exposures to ${\gamma}$-irradiation of $Co^{60}$, ultraviolet, and four repeated treatments with Nmethyl-N'-nitrosoguanidine. The mutant strain Aspergillus sp. XN15 was chosen after a rigorous screening process, with its production of the raw-starch-digesting enzyme being twice that of usual wild varieties cultured under preoptimized conditions and in an unsupplemented medium. After 17 successive subculturings, the enzyme production of the mutant was stable. Optimal conditions for the production of the enzyme by solid-state fermentation, using wheat bran as the substrate, were accomplished for the mutant Aspergillus sp. XN15. With the optimal fermentation conditions, and a solid medium supplemented with nitrogen sources of 1% urea and 1% $NH_4NO_3$, 2.5 mM $CoSO_4$, 0.05% (v/w) Tween 80, and 1% glucose, the mutant Aspergillus sp. XN15 produced the raw-starch-digesting enzyme in quantities 19.4 times greater than a typical wild variety. Finally, XN15, through simultaneous saccharification and fermentation of a raw rice corn starch slurry, produced a high level of ethanol with $Y_{p/s}$ of 0.47 g/g.