Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation

  • Vu, Van Hanh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Pham, Tuan Anh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
  • Received : 2010.11.10
  • Accepted : 2010.12.13
  • Published : 2011.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with $Co^{60}$ ${\gamma}$-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or $NH_4Cl$, 1% (w/w) rice starch, 2.5 mM $MgCl_2$, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at $35^{\circ}C$ for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.

Keywords

References

  1. Duff SJ, Murray WD. Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Bioresour Technol 1996;55:1-33. https://doi.org/10.1016/0960-8524(95)00122-0
  2. Jones RP, Pamment N, Greenfield PF. Alcohol fermentation by yeasts: the effect of environmental and other variables. Process Biochem 1981;16:42-9.
  3. Rogers PL, Lee KJ, Tribe DE. High productivity ethanol fermentation with Zymomonas mobilis. Process Biochem 1980;15:7-11.
  4. Shin D, Yoo A, Kim SW, Yang DR. Cybernetic modeling of simultaneous saccharification and fermentation for ethanol production from steam-exploded wood with Brettanomyces custersii. J Microbiol Biotechnol 2006;16:1355-61.
  5. Durand H, Clanet M, Tiraby G. Genetic improvement of Trichoderma reesei for large scale cellulase production. Enzyme Microb Technol 1988;10:341-6. https://doi.org/10.1016/0141-0229(88)90012-9
  6. Kang SW, Park YS, Lee JS, Hong SI, Kim SW. Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass. Bioresour Technol 2004;91:153-6. https://doi.org/10.1016/S0960-8524(03)00172-X
  7. Sohail M, Siddiqi R, Ahmad A, Khan SA. Cellulase production from Aspergillus niger MS82: effect of temperature and pH. New Biotechnol 2009;25:437-41. https://doi.org/10.1016/j.nbt.2009.02.002
  8. Sun Y, Cheng JY. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 2002;83:1-11. https://doi.org/10.1016/S0960-8524(01)00212-7
  9. Zhang YH, Himmel ME, Mielenz JR. Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 2006;24:452-81. https://doi.org/10.1016/j.biotechadv.2006.03.003
  10. Parekh S, Vinci VA, Strobel RJ. Improvement of microbial strains and fermentation processes. Appl Microbiol Biotechnol 2000;54:287-301. https://doi.org/10.1007/s002530000403
  11. Kuhad RC, Kumar M, Singh A. A hypercellulolytic mutant of Fusarium oxysporum. Lett Appl Microbiol 1994;19:397-400. https://doi.org/10.1111/j.1472-765X.1994.tb00486.x
  12. Vu VH, Pham TA, Kim K. Fungal strain improvement for cellulase production using repeated and sequential mutagenesis. Mycobiology 2009;37:267-71. https://doi.org/10.4489/MYCO.2009.37.4.267
  13. Pandey A. Solid-state fermentation. New Delhi: Wiley Eastern Limited; 1994. p. 12-7.
  14. Babu KR, Satyanarayana T. ${\alpha}$-Amylase production by thermophilic Bacilluscoagulans in solid state fermentation. Process Biochem 1995;30:305-9. https://doi.org/10.1016/0032-9592(95)87038-5
  15. Grajek W. Comparative studies on the production of cellulases by thermophilic fungi in submerged and solid-state fermentation. Appl Microbiol Biotechnol 1987;26:126-9. https://doi.org/10.1007/BF00253895
  16. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8. https://doi.org/10.1021/ac60147a030
  17. Rubinder K, Chadha BS, Singh N, Saini HS, Singh S. Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus. J Ind Microbiol Biotechnol 2002;29:70-4. https://doi.org/10.1038/sj.jim.7000270
  18. Vu VH, Pham TA, Kim K. Improvement of a fungal strain by repeated and sequential mutagenesis and optimization of solid-state fermentation for the hyper-production of rawstarch-digesting enzyme. J Microbiol Biotechnol 2010;20:718-26. https://doi.org/10.4014/jmb.0908.08016
  19. Singh A, Abidi AB, Darmwal NS, Agrawal AK. Influence of nutritional factors of cellulase production from natural lignocellulosic residues by Aspergillus niger. Agric Biol Res 1991;7:19-27.
  20. Asgher M, Asad MJ, Legge RL. Enhanced lignin peroxidase synthesis by Phanerochaete chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World J Microbiol Biotechnol 2006;22:449-53. https://doi.org/10.1007/s11274-005-9055-7
  21. Frausto da Silva JJ, Williams RJ. The biological chemistry of the elements: the inorganic chemistry of life. New York: Clarendon Press; 1993.

Cited by

  1. Statistical optimization of cellulases production by Penicillium chrysogenum QML-2 under solid-state fermentation and primary application to chitosan hydrolysis vol.28, pp.3, 2012, https://doi.org/10.1007/s11274-011-0919-8
  2. Improvement of Aspergillus oryzae NRRL 3484 by mutagenesis and optimization of culture conditions in solid-state fermentation for the hyper-production of extracellular cellulase vol.106, pp.5, 2014, https://doi.org/10.1007/s10482-014-0255-8
  3. Fermentation Optimization and Unstructured Kinetic Model for Cellulase Production by Rhizopus stolonifer var. reflexus TP-02 on Agriculture By-Products vol.177, pp.8, 2015, https://doi.org/10.1007/s12010-015-1839-0
  4. Mutagenesis breeding of high echinocandin B producing strain and further titer improvement with culture medium optimization vol.38, pp.10, 2015, https://doi.org/10.1007/s00449-015-1425-4
  5. Cellulase Production from Species of Fungi and Bacteria from Agricultural Wastes and Its Utilization in Industry: A Review vol.04, pp.02, 2016, https://doi.org/10.4236/aer.2016.42005
  6. The use of Amazon fungus (Trametes sp.) in the production of cellulase and xylanase vol.15, pp.20, 2016, https://doi.org/10.5897/AJB2015.14624
  7. Cost-effective cellulase production using Parthenium hysterophorus biomass as an unconventional lignocellulosic substrate vol.7, pp.1, 2017, https://doi.org/10.1007/s13205-017-0604-1
  8. Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01 vol.7, pp.3, 2017, https://doi.org/10.1007/s13205-017-0755-0
  9. Cellulase Enzyme Production From Rice Straw Using Solid State Fermentation and Fungi Aspergillus niger ITBCC L74 vol.156, pp.2261-236X, 2018, https://doi.org/10.1051/matecconf/201815601010
  10. IIPC 324 under SSF via saccharification of acid-pretreated sugarcane bagasse pp.1759-7277, 2021, https://doi.org/10.1080/17597269.2018.1449063
  11. Efficacy and cost study of green fungicide formulated from crude beta-glucosidase pp.1735-2630, 2018, https://doi.org/10.1007/s13762-018-2084-1
  12. Screening of potential IL-tolerant cellulases and their efficient saccharification of IL-pretreated lignocelluloses vol.8, pp.54, 2018, https://doi.org/10.1039/C8RA05729J
  13. under low moisture conditions during solid-state fermentation vol.68, pp.2, 2019, https://doi.org/10.1111/lam.13104