• Title/Summary/Keyword: strain 320

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Isolation and Cultural Characteristics of Styrene Dimer [Endocrine Disrupter] Biodegrading Microorganism (Styrene dimer [환경호르몬 물질] 분해균주의 분리 및 배양특성)

  • ;;;Saido Katsuhiko
    • KSBB Journal
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    • v.19 no.4
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    • pp.315-320
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    • 2004
  • We examined the culture conditions and degrading characteristics of styrene dimer (endocrine disrupter) using microorganism. The isolated microbe were consisted of 3 kinds of strain. The strains were identified to Pseudomonas sp. and Klebsiella pneumoniae by API 20E kit, but one was not identified. Single strain was not grown on the C-medium containing styrene dimer. However the complex strain YH3 could grow and we confirmed it by the broth color and O.D$_{660nm}$ (optical density 660 nm). The optimal culture conditions of complex strain YH3 were 35$^{\circ}C$, 1,000 ppm (v/v) of styrene dimer and pH 7.0, respectively. In tolerance test against the organic solvents, the complex strain YH3 could grow above log P=3.1, and could degrade ethyl benzene and 2,4-D, one kind of herbicide. As a result of TLC (Thin Layer Chromatography) analysis, we confirmed that the metabolite of styrene dimer was created by YH3 after 5th day, but not at control samples.

Texture and Plastic Strain Ratio Changes during a 2 Step Asymmetric Rolling and Annealing of AA5083 Al Alloy Sheet (2단계 비대칭 압연과 열처리한 AA5083 Al 합금판재의 집합조직과 소성변형비 변화)

  • Jeong, H.B.;Lee, J.H.;Kim, G.H.;Nam, S.K.;Kim, I.
    • Transactions of Materials Processing
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    • v.23 no.2
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    • pp.82-87
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    • 2014
  • The plastic strain ratio is one of the factors that affect the deep drawability of Al alloy sheet. The deep drawability of Al alloy sheet is limited because of its low plastic strain ratio. Therefore an increase in the plastic strain ratio to improve the deep drawability of Al alloy sheet is needed. The current study investigated the increase of the plastic strain ratio and the change in texture of AA5083 Al alloy sheet after a 2 step asymmetric rolling with heat treatments. The average plastic strain ratio of initial AA5083 Al alloy sheets was 0.83. After the first asymmetric rolling step of 88% deformation and subsequent heat treatment at $320^{\circ}C$ for 10 minutes the value was still 0.83. After the second asymmetric rolling of 14% reduction and subsequent heat treatment at $330^{\circ}C$ for 10 minutes the plastic strain ratio rose to 1.01. The average plastic strain ratio after the 2 step asymmetric rolling and heat treatment is 1.2 times higher than that of initial AA5083 Al alloy sheet. This result is related to the development of ND/<111> texture component after the second asymmetric rolling and heat treatment.

Yielding Behavior and Strain Aging Properties of Bake Hardening Steel with Dual-Phase Microstructure (2상 조직을 갖는 소부경화강의 항복 거동과 변형 시효 특성)

  • Lee, Seung-Wan;Lee, Sang-In;Hwang, Byoungchul
    • Korean Journal of Materials Research
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    • v.30 no.6
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    • pp.315-320
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    • 2020
  • This study deals with the yielding behavior and strain aging properties of three bake hardening steels with dual-phase microstructure, fabricated by varying the annealing temperature. Bake hardening and aging tests are performed to examine the correlation of martensite volume fraction with yielding behavior and strain aging properties of the bake hardening steels with dual-phase microstructure. The volume fraction of martensite increases with increasing annealing temperature. Room-temperature tensile test results show that the yielding behavior changes from discontinuous-type to continuous-type with increasing volume fraction of martensite due to higher mobile dislocation density. According to the bake hardening and aging tests, the specimen with the highest fraction of martensite exhibited high bake hardening with low aging index because solute carbon atoms in ferrite and martensite effectively diffuse to dislocations during the bake hardening test, while in the aging test they diffuse at only ferrite due to lower aging temperature.

Behavior of Small-Scale Pile Group Under Vertical Loading (연직하중을 받는 소규모 무리말뚝의 거동)

  • 이영남;이승현;박영호
    • Journal of the Korean Geotechnical Society
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    • v.17 no.6
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    • pp.37-46
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    • 2001
  • Pile load tests were carried out to investigate the contribution of the pile cap to the carrying capacity of a pile group and load transfer characteristics of piles in the group. A group of 24 piles$(4 \times6 array)$ of 92.5mm diameter steel pipe were installed to the depth of 3m fron the ground surface, the top of weathered rock. A maximum load of 320ton was applied to the pile cap, $1.5\times2.3m$, in contact with the ground surface. At the maximum load of 320ton, the pile cap has carried 22% of the total load. Average ultimate capacity of pile in the pile group was estimated to be 16.4ton, substantially higher than that of single pile, installed at the corner and tested before pile cap construction. For the same magnitude of settlement, the pile in the center carried less load than the pile at the perimeter due to strain superposition effect. Piles in the group showed almost constant contribution(approx. 60%) of side friction to the total capacity for all of the loading stages, while that of single pile decreased from 82% to 65%.

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Shear Strength of High Strength Concrete Beams with Steel Fibrous (강섬유를 혼입한 고강도 콘크리트 보의 전단강도)

  • 곽계환;박종건;정태영
    • Journal of the Korea Concrete Institute
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    • v.12 no.4
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    • pp.23-30
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    • 2000
  • The purpose of this paper is to study on the shear strength of high strength concrete beams with steel fibrous. In general, the shear strength of reinforced concrete beams is affected by the compressive strengths of concrete( c), the shear span-depth ratio(a/d), the longitudinal steel ratio($\rho$ $\omega$), and shear reinforcement. An experimental investigation of the shear strength of high strength concrete beams with steel fibrous was conducted. In each series the shear span-depth ratio(a/d) was held constant at 1.5, 2.8, or 3.6, while concrete strengths were varied from 320 to 520, to 800kgf/$\textrm{cm}^2$. To verify the proposed equations the experimental results were compared with those from other researches such as equation of ACI code 318-95 or equation of Zsutty. To deduce equation for shear strength from experimental data carried out MINITAP program. According to the experimental results, the addition of steel fibrous has increased the deflection and strain at failure load, improving the brittleness of the high strength concrete.

Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom

  • Le, Ve Van;Ko, So-Ra;Lee, Sang-Ah;Kang, Mingyeong;Oh, Hee-Mock;Ahn, Chi-Yong
    • Journal of Microbiology and Biotechnology
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    • v.32 no.5
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    • pp.575-581
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    • 2022
  • A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4T, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4T was most closely related to Caenimonas terrae SGM1-15T and Caenimonas koreensis EMB320T with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4T and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C16:0, cyclo C17:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). Strain DR4-4T possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4T represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4T (=KCTC 82470T =JCM 34453T).

Shear Behavior of Polymer Cement High Strength Concrete Beams Mixed with Steel Fiber (강섬유 혼입 폴리머 시멘트 고강도 콘크리트 보의 전단거동)

  • 곽계환;박종건;곽경헌
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.44 no.1
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    • pp.93-102
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    • 2002
  • Steel fiber and polymer are used widely for reinforcement material of RC structures because of its excellences of the durability, serviceability as well as mechanical properties. The purpose of this study is to investigate the shear behavior of polymer cement high strength concrete beams mixed with steel fiber. The compressive strength of concrete was based on the 100$\times$200 mm cylinder specimens. The compressive strength of concrete are 320$kgf/cm^2$, 436 $kgf/cm^2$ and 520 $kgf/cm^2$ in the 28 days. The static test was carried out to measure the ultimate load, the initial load of flexural and diagonal cracking, crack patterns and fracture modes. Also, load-strain and load-deflection examined. During the test cracks were sketched against the load values according to the growth of crack. result are as follows; (1) The failure modes of the specimens are increased in rigidity and durability with mixing steel fiber and polymer. (2) The load of initial crack was similar a theory of shear-crack strength. (3) The deflection and strain at failure load of Polymer-steel fiber high strength concrete beams were increased, improving the brittleness of the high strength concrete.

An Experimental Study on Shear Behavior of Polymer-Steel Fibrous High Strength Concrete Beams (폴리머-강섬유를 혼입한 고강도 콘크리트보의 전단거동에 관한 실험적 연구)

  • 곽계환;조선정;김원태;조한용
    • Proceedings of the Korea Concrete Institute Conference
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    • 2000.10a
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    • pp.601-608
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    • 2000
  • Steel fiber and Polymer are used widely for the reinforcement material of RC structures because of its excellence of durability, serviceability as well as mechanical properties. Polymer-Steel fibrous high strength concrete beam's input ratio are 1.0%. The shear span-to-depth ratio are 1.5, 2.8 and 3.6, compressive strength of specimens 320kg/㎠, 436kgf/㎠ and 520kgf/㎠ in 28 days. The static test was carried out to measure the ultimate load, the initial load of flexural crack and of diagonal crack, from which crack patte군 and fracture modes are earned. Also, stress-strain, load-strain and load-deflection are examined during the test cracks(shear crack, flexural crack, and diagonal tension crack), when the load values are sketched according to the growth of crack. Result are as follows; (1) The failure modes of the specimens increase in rigidity and durability in accordance with the increase of mixing steel fiber and polymer. (2) The load of initial crack was the same as the theory of shear-crack strength (3) Polymer-Steel fibrous high strength concrete beams have increased the deflection and strain at failure load, improving the brittleness of the high strength concrete. (4) In this result of study, an additional study need to make a need formular because the study is different from ACI formular and Zsutty formular.

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Isolation and Characteristics of Biosurfactant Producing Bacterium, Bacillus sp. TBM 911-5 (Biosurfactant 생산균주 Bacillus sp. TBM 911-5의 분리 및 특성)

  • 김선희;정연주;이상철;유주순;주우홍;정수열;최시림;최용락
    • Journal of Life Science
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    • v.14 no.2
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    • pp.320-324
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    • 2004
  • The objective of this study was investigate the characteristic of biosurfactant produced from the isolated strain. The strain was isolated from soil samples and identified as Bacillus sp. TBM 911-5 by physiological characteristics and the partial nucleotide sequence analysis of 16S rDNA. We measured the surface tension every 6 hours for 80 hours. The surface tension of the culture filtrate of Bacillus sp. TBM 911-5 was decreased to 29 mN/m. Biosurfactant concentration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The biosurfactant emulsified hydrocarbons, vegetable oil and crude oil. Using soybean oil as substrate, the maximum emulsification activity and stability was obtained from the biosurfactant. The biosurfactant produced from Bacillus sp. TBM 911-5 had strong properties as an emulsifying agent and an emulsion-stabilizing agent.

Autogenous Shrinkage of High-Performance Concrete Containing Mineral Admixture (광물질 혼화재를 함유한 고성능 콘크리트의 자기수축)

  • Lee, Chang-Soo;Park, Jong-Hyok;Kim, Yong-Hyok;Kim, Young-Ook
    • Journal of the Korean Society of Hazard Mitigation
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    • v.7 no.3
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    • pp.19-31
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    • 2007
  • Humidity and strain were estimated for understanding the relation between humidity change by self-desiccation and shrinkage in high-performance concrete with low water binder ratio and containing fly ash and blast furnace slag. Internal humidity change and shrinkage strain were about 10%, 10%, 7%, 11%, 11% and $320{\times}10^{-6}$, $270{\times}10^{-6}$, $231{\times}10^{-6}$, $371{\times}10^{-6}$, $350{\times}10^{-6}$ respectively on OPC30, O30F10, O30F20, O30G40, O30G50 and from the results, fly ash made humidity change and strain decrease but slag increase comparing with ordinary portland cement. Considering only relation internal humidity and shrinkage by self-desiccation, humidity change and shrinkage represented the strong linear relation regardless of mineral admixture. For specifying the relation on internal humidity change and autogenous shrinkage strain, shrinkage model was established which is driven by capillary pressure in pore water and surface energy in hydrates on the assumption of a single network and extended meniscus in pore system of concrete. This model and experimental results had a similar tendency so it would be concluded that the internal humidity change by self-desiccation in HPC originated in small pores less than 20nm, therefore controlling plan on autogenous shrinkage might be focused on surface tension of water and degree of saturation in small pore.