• Journal of the Korean GEO-environmental Society
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• v.11 no.1
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• pp.13-18
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• 2010

It is crucial to decide reasonably the parameters necessary for design through research on the characteristics of rock in order to analyze stability of rock structure. This article has conducted both pressure meter test, uniaxial and triaxial compression test to the areas of andesite or tuff located in local regions such as Yeosu, Gwangju, Yangsan, Busan, and Daejeon and has comparatively analyzed previously proposed estimative formulas. According to the result of estimating the deformation modulus through using the damping coefficient suggested by Nicholson & Bieniawski, when RMR is less than 60, it is desirable to use the result of the pressure meter test considering the damping coefficient. If the RMR value is over 60, however, the formula suggested by Kim Gyo-won has been proved to be the most applicable. Moreover, according to the result of comparing the RMR, adhesion, and angle of internal friction, both the adhesion and angle of internal friction best correspond to the formula proposed by Tsuchiya. Comparatively analyzing the relations between the rock deformation modulus and RMR and also suggesting the formula of calculating the shear strength parameter to use the RMR value, this study did not include the deformation modulus and shear strength parameter as factors to decide the RMR index. Since result can differ by several factors, it will be necessary afterwards to suggest practical estimative formulas applicable to the actual spots of Korea.

• Journal of Environmental Science International
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• v.12 no.12
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• pp.1285-1292
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• 2003

This study was compared each ingredient's local/seasonal change characteristics by analyzing anions and cations, watersoluble ionic components, from the precipitation of Dangjin and Anmyeon-do areas. The samples were collected for 8 months from both spots between the period of April 1, 2002 to November 31, 2002. The precipitation samples were collected continuously through the entire duration of precipitation by using the wet-only automatic samplers. When rain continues to fall over 24 hours or occasionally, we considered those collected from 9 o'clock in the morning for 24 hours as the day's samples. As a method to verify for the reliability of the analyzed data is concerned, we use the ion balance method and the electricity conductance method, was used widely as a way of watching the atmosphere by the WMO(World Meteorological Organization)/GAW (Global Atmosphere Watch). Also, Dangjin and Anmyun-do area confirmed that contains artificial pollutants by analysis of ion concentration data.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.2
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• pp.187-193
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• 2011

Neurofibromatosis is known as an autosomal dominant disorder caused by a mutation of a tumor suppressor gene on the long arm of chromosome 17 that affects the bone, nervous system, soft tissue, skin, and endocrine system. The most characteristic finding, which is helpful for clinical diagnosis as well, is the neurofibroma. Likewise brown macules called Cafe-au-lait spots with color of caffee latte, and Lisch nodules found around iris are useful to diagnose the disease. As known, the possibility of the neurofibromatosis occurred in oral cavity is relatively rare, and in most of cases it is related to soft tissue changes with single or multiple neurofibromatosis. The purpose of this report is to present characteristic dental findings which were found in a 4-year-old male and his father, both diagnosed as neurofibromatosis at Chonbuk National University Hospital before visiting our department, pediatric dentistry.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.71.1-71.1
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• 2013

Among quadruples or higher multiplicity stars, only a few binary systems have been discovered. They are important targets to understand the formation and evolution of multiple stellar systems because we can obtain accurate stellar parameters from photometric and spectroscopic studies. We present the observational results of this kind of rare object 1SWASP J093010.78+533859.5, for which the doubly eclipsing feature had been detected previously from the SuperWASP photometric archive. Individual PSF photometry for two objects with a separation of about 1.9 arcsec was performed for the first time in this study. Our time-series photometric data show that the brighter object A is an Algol-type detached eclipsing binary with an orbital period of 1.3 days and the fainter B is a W UMa-type contact eclipsing binary with a period of 0.23 days. Using the high-resolution optical spectra, we obtained well-defined radial velocity variations of the system A. Furthermore, stationary spectral lines were detected and should have originated from the other stellar component, which was confirmed by the third object contribution from the light curve analysis. No spectral feature of the system B was detected, probably due to its faintness. We obtained the binary parameters and the absolute dimensions from each light curve synthesis. The primary and secondary components of the system A have a spectral type of K1 and K5 main sequences, respectively. Two components of system B have nearly the same type of K3 main sequence. Light variations at out of eclipses were appeared in both systems, interpreting as the effect of stellar spots on these late spectral type stars. We estimated the distances to the systems A and B individually. They may have similar distances of about 70 pc and seem to be gravitationally bound with a separation of about 130 AU. In conclusion, we suggest that 1SWASP J093010.78+533859.5 is a quintuple stellar system with a hierarchical structure of a triple system A(ab)c and a binary system B(ab).

• KSBB Journal
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• v.19 no.4
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• pp.269-273
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• 2004

A simple, fast and reproducible quantitative analysis method for sugar concentration composed in oligosaccharide mixture was developed. Two glass TLC plates were prepared per sample. After dipping one plate into the copper bicinchoninate reagent and the other plate into 5% sulfuric acid solution, both plates were baked in microwave oven until sugar spots were developed or the surface temperature of TLC plate becomes 60 to 70 $^{\circ}C$. The corrective factor values [F value =(the value of total sugar concentration converted as glucose unit/the value of reducing sugar concentration converted as glucose unit)/(polymerization degree of sugar)] of different molecular weight sugars were determined. Within the concentration of 0.25∼1.0 $\mu\textrm{g}$ in each sample loaded, the fructose-F (corrective factor value of fructose) was 0.45, yet for the higher concentration (2.5∼7.5 $\mu\textrm{g}$) fructose-F was 1.0. In case of glucose, in the range of 0.5∼7.5 $\mu\textrm{g}$, glucose-F was same as fructose-F, 1.0. However, as the molecular weight of sugar was increased, the F values were decreased in both maltodextrin and isomaltodextrin oligosaccharides in 0.5∼7.5 $\mu\textrm{g}$ of each sample loaded. Interestingly, F values were equal for the same molecular weight sugars, although the structures were different from each other. Using F value of each sugar, we could determine and compare the exact total sugar concentration of different molecular weight maltooligosaccharide and isomaltooligosaccharide. We also could determine if the unknown sugar was a reducing or non-reducing compound by using optimized TLC with microwave oven method.

• Korean Journal of Environmental Agriculture
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• v.15 no.2
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• pp.217-222
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• 1996

A green-house experiment was conducted to know the performance of Chinese cabbage and radish applied with simulated acid rain(SAR) on the leaf and/or soil. The pHs of SAR and normal water were 2.7 and 6.0, respectively. The pHs of SAR and normal water applied on leaf/soil were 6.0/6.0, 6.0/2.7, 2.7/6.0, and 2.7/2.7 and they were applied fifteen times at the two- or three-day intervals with 10mm at a time. Leaf application of SAR caused brown spots in both crops, while no such symptoms were observed in soil application. SAR applied on the leaf or soil increased chlorophyll content significantly in Chinese cabbage, but slightly in radish. Leaf or soil application of SAR did not affect N, P, and K concentrations in both crops. The sulfur content in radish leaf increased by leaf or soil application of SAR, while in the root it increased by soil application only. Yield of Chinese cabbage was not affected by SAR, while both leaf and root yields of radish were significantly reduced by leaf application of SAR compared with soil application.

• Korean Journal of Environmental Agriculture
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• v.15 no.4
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• pp.419-424
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• 1996

A green house experiment was conducted to investigate the performance of Chinese cabbage and radish and changes in soil chemical properties after application of different amounts of simulated acid rain (SAR). About 10mm of normal water (pH 6.0), 5mm of SAR followed by 5mm of normal water, and 10mm of SAR were applied 24 times at the two-day intervals. The results obtained are summarized as follows : 1. Visible symptoms were dark brown, red brown, or grey brown leaf spots, reduced leaf size, or some times wrinked leaves in both crops. The visible symptoms started after the third application of SAR. The degree of damages was greater as the amounts and the number of times of SAR application increased. 2. Chlorophyll contents of SAR applied leaves decreased as the amounts of SAR application increased. 3. As the amounts of SAR increased, length, diameter, and fresh weight of Chinese cabbage heads and radish roots decreased. 4. In both crops sulfur content increased, while calcium content decreased as the amounts of SAR increased. Contents of N, P, K, and Mg were not significnatly affected by the amounts of SAR. 5. SAR decreased soil pH and exchangeable cations (Ca, Mg, K), while increased soil sulfur content.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.92-102
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• 2006

Abundant proteins often cause problems in proteome study. Glutelin family proteins (hereafter referred to glutelin) are present in rice proteome sample as over-whelming constituents with very high abundance. In order to increase the number of identified proteins in rice proteome study, we developed a newly improved method for sample preparation through the removal of glutelin. When the protein samples from rice seed were extracted by the conventional trichloroacetic acid (TCA) extraction method, glutelin accounts for about 60% of total rice seed proteins in SDS gels. Using our new water extraction method, glutelin consists of only about 10% of total proteins. After analyzing on a two-dimensional gel electrophoresis (2-DE), 937 protein spots were detected using the conventional TCA extraction method. On the other hand, 1240 proteins could be seen using the new water extraction method. The selectivity for non-glutelin and less abundant protein by the water extraction method was also confirmed by ESI-Q/TOF mass spectrometry analysis. Thus, the new water extraction method developed here can be efficiently used to study the proteome analysis of rice storage seed.

• Development and Reproduction
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• v.16 no.4
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• pp.339-351
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• 2012

Fetal bovine serum (FBS) is the most frequently used serum for the cultivation of mammalian cells. However, since animal-derived materials might not be appropriate due to safety issues, allogeneic human serum (HS) has been used to replace FBS, particularly for the culture of human cells. While there has been a debate about the advantages of HS, its precise effect on human adult stem cells have not been clarified. The present study aimed to investigate the effect of HS on the human eyelid adipose stem cells (HEACs) in vitro. When HEACs were cultivated in a medium containing 10% HS, many cells moved into several spots and aggregated there. The phenomenon was observed as early as 9 days following 10% HS treatment, and 12 days following 5% HS plus 5% FBS treatment. However, the aggregation was never observed when the same cells were cultivated with 10% FBS or bovine serum albumin. To examine whether cell density might affect the aggregation, cells were seeded with different densities on 12-well dish. Until the beginning of aggregation, cells seeded at low densities exhibited the longest culture period of 16 days whereas cells seeded at high densities showed the shortest period of 9 days to form aggregation. The number of cells was $15.1{\pm}0.2{\times}10^4$ as the least for the low density group, and $29.3{\pm}2.8{\times}10^4$ as the greatest for the high density group. When human cord blood serum or normal bovine serum was examined for the same effect on HEACs, interestingly, cord blood serum induced the aggregation of cells whereas bovine serum treatment has never induced. When cells were cultivated with 10% HS for 9 days, they were obtained and analyzed by RT-PCR. Compared to FBS-cultivated HEACs, HS-cultivated HEACs did not express VIM, and less expressed GATA4, PALLD. On the other hand, HS-cultivated HEACs expressed MAP2 more than FBS-cultivated HEACs. In conclusion, human adult stem cells could move and form aggregates by the treatment with human body fluids.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.292-297
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• 2015

A new cultivar of Hibiscus 'Red Bohanjae' (Hibiscus syriacus 'Red Bohanjae') was developed after crossing between Hibiscus syriacus 'Andong' (♀) with white flowers and Hibiscus syriacus 'Samchully' (♂) with pink flowers to improve flower quality and growth habit. The Hibiscus breeding program was initiated in 2002, and H. syriacus 'Red Bohanjae' was preliminarily selected as '02-RV-17' in 2003 due to its stable flower quality with rich pink flowers and small tree type. The selected line was further evaluated for growth characteristics, leaf shape, leaf size, flowering characteristics, and tested for distinctness, homogeneity, and stability during 2004-2007. The characteristics preserved by grafting. 'Red Bohanjae' showed a small growth habit with slightly upright branches. The tree had potential use as a potted plant or bonsai because of its dwarf form. The new cultivar 'Red Bohanjae' had violet-pink color flowers (RHS N78C) with red eye spots, small size (9.2 cm) and spatulate (I-type) petals. Leaves were 4.2 cm long and 2.3 cm wide. After the evaluation of plant characteristics for 3 years (2005-2007), it was registered as cultivar 'Red Bohanjae' (4118, plant variety protection rights number) in 2012. 'Red Bohanjae' having violet-pink flowers with red eye and dwarf tree form seems to be suitable for pot or bonsai cultivation in both indoor and outdoor conditions.