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Fabrication of Electrospun Juniperus Chinensis Extracts loaded PVA Nanofibers (향나무 추출물을 함유하는 PVA 나노섬유 제조)

  • Kim, Jeong-Hwa;Lee, Jung-Soon
    • Science of Emotion and Sensibility
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    • v.19 no.2
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    • pp.35-42
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    • 2016
  • Electrospinning is a simple and effective process for producing nanofiber with diameter range from nanometers to micrometers which have high specific surface area. Hence, medicated nanofibers can be readily fabricated using a solution containing a mixture of a plant-extracts and a polymer. It has proved that Juniperus Chinensis can be effectively used for the prevention of UV and SLS-induced advers skin reaction such as radical production, inflammation and skin cell damage. It also found that Juniperus Chinensis has efficient ingredient of antifungal activity and house dust mite repellent effect. The fabrication of PVA nanofibers containing Juniperus Chinensis extracts by electrospinning has been studied. PVA/Juniperus Chinensis extracts composite nanofibers were produced at different Juniperus Chinensis concentrations (0.25, 0.5, 1.5 wt. %). The parameters of electrospinning including polymer contents, voltage and tip-to-collector distance (TCD) were optimized for fabrication process. The study show that 12 wt. % PVA, 10kV applied voltage and TCD 10~20 cm are the best condition to obtain uniform PVA/Juniperus Chinensis extracts composite nanofibers. Morphologies of the electrospun composite nanofiber were observed by using a field emission scanning electron microscope. It has been found that the average diameters of fibers increased by the adding of Juniperus Chinensis extracts. As the results, PVA/Juniperus Chinensis extracts composite nanofibers having a diameter in the range from 310~360 nm were successfully prepared via an electrospinning.

Dietary Intake Status among Korean Female Breast Cancer Survivors (한국 여성 유방암 경험자의 식사섭취실태)

  • Park, Myungsook;Hwang, Eunkyung;Moon, Hyeong-Gon;Noh, Dong-Young;Lee, Jung Eun
    • Korean Journal of Community Nutrition
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    • v.19 no.2
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    • pp.163-175
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    • 2014
  • Objectives: We aimed to evaluate dietary intake among female breast cancer survivors in a cross-sectional study. Methods: A total of 127 women who had breast cancer surgery at least 6 months before baseline were included. Dietary intake of female breast cancer survivors was assessed through self-reported 3 day-dietary records. To compare dietary intake between breast cancer survivors and general female population without cancer, we selected the 1:4 age matched women from the 2011 Korean National Health and Nutrition Examination Survey (KNHANES). In the KNHANES, participants were asked about their dietary intake using the 24-hour dietary recalls. We also examined whether dietary intake varied by age group, cancer stage, or time since surgery among breast cancer survivors. We used the generalized linear model to compare their dietary intakes. Results: Intakes of total energy, beta-carotene, folate, vitamin C, plant iron and fruits were lower among breast cancer survivors with longer time since surgery compared to those with shorter time (p<0.05). Breast cancer patients with higher stage at diagnosis tended to consume less legumes (p=0.01) than those with lower stage. When we compared dietary intake between breast cancer survivors and the general female population without cancer, breast cancer survivors were more likely to consume most of macro- and micro-nutrients in larger quantity (p<0.05) and adhere to healthier diet characterized by higher intakes of legumes, seed and nuts, vegetables and fishes and shells than the general female population who never had been diagnosed with cancer (p<0.05). Conclusions: Our study results suggested that the intakes of nutrients and foods varied by time since surgery and cancer stage among breast cancer survivors and dietary intakes among breast cancer survivors differed from that in the general population. Further prospective studies are warranted to explore the association between dietary intakes of specific food items and survival among Korean breast cancer survivors.

Study on Local Wireless Network Data Structure for Sludge Multimeter (슬러지 멀티미터를 위한 근거리무선네트워크 데이터구조 설계 연구)

  • Jung, Soonho;Kim, Younggi;Lee, Sijin;Lee, Sunghwa;Park, Taejun;Byun, Doogyoon;Cha, Jaesang
    • Journal of Satellite, Information and Communications
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    • v.9 no.2
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    • pp.96-100
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    • 2014
  • Recently, the management system of wastewater treatment facility has magnified due to the stringent regulations for the protection of the environment, and a sewage treatment plant efficiency and research of the car development are activated in large facilities or industrial park. however, the existing sewerage disposal system and specific water quality monitoring network reliability for real-time transmission of this building is insufficient. In this paper, we proposed a local wireless network design for sludge multi meter data collection and control for measuring the concentration of the sludge efficiently. Also, the collected data over the local wireless network to transmitted to the central monitoring system and accumulate the data in real time to calculate statistics is possible to monitor the status of the sewage treatment facilities. The proposed system uses a short-range wireless networks of IEEE 802.15.4 and configures an IEEE 802.11 network which can monitor real-time status in central system. Also, we install a sludge multimeter and communication network in sewage treatment facilities and confirm the usefulness of the proposed technique by demonstrating its effectiveness.

Effects of Some Plant Growth Regulators on Protein Biosynthesis of Carrot Cells (당근 세포의 단백질 생합성에 대한 몇가지 식물생장조절제의 영향)

  • Yoo, Ki-Jung;Park, Chang-Kyu;Song, Tae-Chul
    • Applied Biological Chemistry
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    • v.28 no.3
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    • pp.187-195
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    • 1985
  • Electrophoretic studies of protein extracts from carrot calluses suspension-cultured on the media containing kinetin, BA, IAA, NAA or $GA_3$ at the levels of $10^{-6},\;10^{-5},\;10^{-4}M$, respectively, were performed to identify polypeptides and proteins regulated by auxin, cytokinin or GA. Fifteen bands of polypeptide(s) were observed in the callus cultured in the control medium devoid of growth regulators, and their molecular weights were $18._4,\;20._2,\;20._0,\;34._9,\;35._7,\;37._4,\;40._3,\;42._2,\;44._1,\;44._4,\;49._3,\;55._0,\;56._6,\;58._1,\;and\;59._9\;KD$, respectively. The synthesis of polypeptide appeared to be promoted in two bands by kinetin, in six bands by BA, in one band by IAA, in two bands by NAA, and in four bands by $GA_3$, while inhibited in five bands by kinetin, in three bands by BA, in four bands by IAA, in three bands by NAA and in three bands by $GA_3$. The polypeptides of $40._3\;KD\;42._2\;KD$ seemed to be regulated by cytokinins, and those of $44._1\;KD,37._4\;KD,\;and\;56._6\;KD$ by auxins. The proteins of three bands with relative mobilities of 0.56, 0.84, and 0.92, respectively, increased in the calluses cultured on the media containing kinetin, IAA, $GA_3$, NAA or BA, compared to the control, but it was difficult to identify the proteins specific for each growth regulator.

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Proteomic Changes in Odae Polished White Rice Grown at Different Cultivation Conditions (재배환경에 따라 변화하는 오대벼 백미의 단백질체 분석)

  • Lee, Ju-Young;Lee, Jin-Woo;Kim, Young-Ran;Yeom, Yu-Jin;Lim, Jin-Kyu
    • Journal of Applied Biological Chemistry
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    • v.55 no.2
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    • pp.79-83
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    • 2012
  • It has been known that the proteome profiles in the period of growth and development of rice are changed by the growth conditions including temperature, soil, and fertilization. In this study, the proteome profiles of Odae polished white rice grown in Chulwon and Chilgog were compared on 2-dimensional(D) gels. The differentially expressed proteins were selected from the 112 identified total proteins and classified into functional groups. The most significantly differentially expressed proteins were stress responsive proteins; Ent-kaur-16-ene synthase, which is responsible for synthesizing a plant hormone gibberellin, was expressed in Chulwon rice and heat shock proteins were in Chilgog rice, respectively. Xylanase inhibitor protein, which inhibits the enzyme xylanase produced by pathogenic fungi and Bacilli, was expressed significantly high in Chilgog rice grown at high temperature. Differential expressions of transporter proteins were observed both in Chulwon and Chilgog rice. Regarding the facts that Chilgog rice contained relatively higher amount of proteins than Chulwon rice and Chulwon rice showed large number of proteins were differentially expressed, it can be concluded that different cultivation conditions could change the protein expression profiles in rice in various ways, including elevation of protein amount or differential expressions of specific proteins, etc. The results suggest that the characteristics of the profiles of the proteome in the polished white rice are definitely changed by the environmental factors including high temperature. The results can be utilized for the development of the proper cultivation conditions for the production of high quality rice with good palatability.

Beneficial Roles of Azospirillum as Potential Bioinoculant for Eco-Friendly Agriculture (친환경농업을 위한 유용미생물 Azospirillum의 효율적 이용)

  • Gadagi, Ravi;Park, Myoung-Su;Lee, Hyoung-Seok;Seshadri, Sundaram;Chung, Jong-Bae;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.5
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    • pp.290-303
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    • 2003
  • Modern agriculture has been heavily dependent on chemical fertilizers to meet the food demands of ever increasing population. Progressive depletion of major plant nutrients in soil due to intensive cultivation practices has also necessitated the use of higher dose of chemical fertilizers particularly in soils where the organic matter content is very low. Indiscriminate use of chemical fertilizers and pressure on agriculturists to enhance per area crop yields has led to fast depletion of fossil fuel resources with concomitant increase in the prices of chemical fertilizers and also led to environmental pollution. Hence, the current trend throughout the world is to explore the possibility of using alternate nutrient sources or increasing the efficiency of chemical fertilizers by supplementing them with organic fertilizers and bioinoculants comprising largely microbes like, bacteria, fungi, algae etc to enhance nitrogen and phosphates in the soil thus creating a sustainable agricultural environment. Among the different microbial inoculants or biofertilizers, Azospirillum could be a potential candidate due to its non specific host root colonization. It had the capability to fix $N_2$ in wide pH regimes and even in presence of combined nitrogen. Azospirillum inoculation can increase the crop yield to 10-25% and substitute 25% of recommended doses of nitrogenous fertilizers. Apart from nitrogen fixation, Azospirillum is also involved in the root improvement, the activity which was attributed to an increase in the rate of water and mineral uptake by roots. The ability of Azospirillum to produce phytohormones was reported to enhance the root respiration rate, metabolism and root proliferation. They have also been reported to produce polyhydroxybutyrate, that can be used as a biodegradable thermosplastic. A lot of studies have addressed improvements in enhancing its efficiency to fix nitrogen fixation and hormone production.

Canine Renal Failure Caused by Ochratoxin A and Citrinin in the Commercial Dog Food (시판 사료에 오염된 Ochratoxin A와 Citrinin에 의한 개의 신부전)

  • Ahn, So-Jeo;Jeoung, Seok-Young;Lim, Man-Su;Park, Son-Il;Han, Jeong-Hee;Kim, Doo
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.82-87
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    • 2007
  • Five dogs with renal failure were referred to the Veterinary Medical Teaching Hospital at Kangwon National University. These dogs had the common history of consumption of Pedigree dry dog food produced in Thailand plant for over 1 month. The dogs showed anorexia, emaciation, vomiting, and polydipsia/polyuria. And in one severely affected dog, bloody diarrhea and hypothermia were seen. The remarkable clinicopathological signs were high value of BUN and creatinine. In some dogs, GGT, phosphorus and lipase were increased. However, no significant changes of complete blood count were found. In urinalysis, hematuria, low specific gravity urine, proteinuria, and calcium oxalate-like crystals were observed. Two severely affected dogs were died. The remained dogs were recovered gradually after change of dog food and supportive therapy. Pathological findings were seen typically in kidneys. Renal atrophy, congestion of the glomerular capillary, and diffuse degeneration, necrosis, dystrophic calcification and regeneration in the tubular epithelium were seen. Yellowish brown fluorolucent laminated materials or particles were quite often found in the lumina of the necrotizing renal tubules of cortex and medulla. Proliferation of fibrous tissue in the interstitium was also seen. By the mycotoxin analysis of the Pedigree dry dog food, ochratoxin A (OTA) and citrinin were detected as much as the concentration of 372.8 ppb and 8.3 ppb, respectively. The final diagnosis of renal failure caused by OTA and citrinin toxicosis was made on the basis of history takings, clinical signs, clinicopathological and pathological findings, and analysis of mycotoxins.

Interpretation of Weeding Efficacy by Mixture Use of Herbicide Combination, Oxyfluorfen and Glyphosate (Oxyfluorfen과 Glyphosate 조합처리(組合處理) 모형(模型)의 혼용효과(混用效果)에 대한 해석적연구(解釋的硏究))

  • Guh, J.O.;Cho, Y.W.;Lee, K.H.
    • Korean Journal of Weed Science
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    • v.7 no.2
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    • pp.236-242
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    • 1987
  • The study was conducted to interprete the fluctuation of weed vegetation in plant-sociological impacts as affected by the mixture use of oxyfluorfen and glyphosate with various dosages. Also, intended to know the real interaction between two herbicides in weeding efficacies. The better efficacy from the above mixture was recognized than from the oxyfluorfen + paraquat mixture on the perennial-sites. In lower rate mixture of oxyfluorfen, the dominance index was increased by the annual grass species (ie. Digitaria), and of glyphosate by the biennial Stellaria and perennial species (ie. Artemisia). Also, the positive maximum action of both oxyfluorfen and glyphosate in various mixture rates was categorized upto 0.55kg ai/ha for oxyfluorfen and 0.35kg ai/ha for glyphosate, respectively. However, the interaction between the above two herbicides recognized actually as negative. Consequently, the use of mixture compound of oxyfluorfen with glyphosate are expected rather to promote the control efficacy of specific weed species, to enlarge the weeding spectrum and to prolong the weeding periods than to reduce the application rate of both chemicals depending on any synergic interactions.

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Effect of Some Herbal Plant Extracts on the Activation of Dendritic Cells (일부 한약재의 수지상세포 활성화 효과)

  • Kim, Do-Soon;Park, Jung-Eun;Cho, Hyun-Wook;Joo, Woo-Hong;Yee, Sung-Tae
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.427-434
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    • 2007
  • Dendritic cells (DCs) play a pivotal roles in the initiation of T cell-mediated immune responses, making them an attractive in immuno vaccines. Angelica gigas and Cnidium officinale were a medicinal herb widely used in Asian countries. In this study, we examined the effects of A. gigas and C. officinale extracts on the DCs functional maturation and phono-type. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated with OVA in the presence or absence A. gigas and C. officinale extracts, respectively, for 24 hours. The antigen-presenting capacity of A. gigas and C. officinale extracts-treated DCs as analyzed by $CD4^+$ helper T cell clone (OVA-specific) proliferation and cytokines (IL-2 and $IFN-{\gamma}$) production were significantly increased. But A. gigas and C. officinale extracts were not directly effected $CD4^+$ helper T cell clone function. Also, the expression of surface co-stimulatory molecules, including major histocompatibility complex (MHC) class II, CD86 and CD11c, is increased on DCs that were stimulated with A. gigas and C. officinale extracts. These results indicate the immunomodulatory properties of A. gigas and C. officinale extracts, which might be medical supplies or health foods.

Features in Using and Planting of Japanese Apricot(Prunus mume) Adopted to Traditional Gardens (전통원림에 도입된 매화(Prunus mume)의 이용과 배식 특성)

  • Lim, Eui-Je;So, Hyun-Su
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.3
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    • pp.68-79
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    • 2012
  • This study examines features in using Japanese apricots that assume a leading place with a special symbolism among various species of trees adopted to traditional gardens from a landscaping view. Specific aspects of how Japanese apricots were adopted to traditional gardens are as follows. First, while Japanese apricots are used in modern landscape space, there traditionally were six types in use of Japanese apricots as Tammae(探梅) Jungmae(庭梅) Bunmae(盆梅) Byoungmae(甁梅) Chomae(造梅) and Mukmae(墨梅). Second, Japanese apricots planted in gardens[庭梅] were placed mainly in a front yard of Sarang-Chae(the men's part of a traditional house) took a central area because symbolism and ornamental values of Japanese apricots suited what classical scholars sought. Preferred planting sites at that time include south of the building, the yard of Sarang-Chae, space in front of windows and next to fences and waterside. Third, Japanese apricots in traditional gardens were planted in five ways; Dansik(單植) Daesik(對植) Gunsik(群植) Yeolsik(列植) Honsik(混植). Dansik was the most common way in planting Japanese apricots and Daesik intended to enhance orderedness and symbolism of space. Japanese apricots for appreciating in an open field took the form of Gunsik in general but Honsik was also used with species of special symbolism. This study offers an opportunity to grasp traditional planting methods of Japanese apricots based on understanding of cultural aspects of Japanese apricots by focusing on Japanese apricots, the representative traditional species of trees. Results of the study would help to recognize the need to newly understand Japanese apricots and be useful information to plant and use Japanese apricots in a traditional way.