• ALGAE
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• v.31 no.3
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• pp.219-230
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• 2016

The Euglena deses group are common freshwater species composed of E. adhaerens, E. carterae, E. deses, E. mutabilis, and E. satelles. These species are characterized by elongated cylindrical worm-like cell bodies and numerous discoid chloroplasts with a naked pyrenoid. To understand the cryptic diversity, species delimitation and phylogenetic relationships among members of the group, we analyzed morphological data (light and scanning electron microscopy) and molecular data (nuclear small subunit [SSU] and large subunit [LSU] rDNAs and plastid SSU and LSU rDNAs). Bayesian and maximum likelihood analyses based on the combined four-gene dataset resulted in a tree consisting of two major clades within the group. The first clade was composed of two subclades: the E. mutabilis subclade, and the E. satelles, E. carterae, and E. adhaerens subclade. The E. mutabilis subclade was characterized by a lateral canal opening at the anterior end and a single pellicular stria, whereas the E. satelles, E. carterae, and E. adhaerens subclade was characterized by an apical canal opening at the anterior end of the cell and double pellicular striae. The second clade consisted of 20 strains of E. deses, characterizing by a subapical canal opening at the anterior end and double pellicular striae, but they showed cell size variation and high genetic diversity. Species boundaries were tested using a Bayesian multi-locus species delimitation method, resulting in the recognition of five cryptic species within E. deses clade.

• Mycobiology
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• v.49 no.4
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• pp.308-345
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• 2021

Lactifluus (Pers.) Roussel is an ectomycorrhizal genus that was recently recognized to be distinct from the genus Lactarius. To date, 226 Lactifluus species have been reported worldwide. Misidentification of Lactifluus species is common because of intraspecific morphological variation, cryptic diversity, and the limited number of taxonomic keys available. Molecular data are indispensable for species delimitation; a multilocus phylogenetic analysis showed that most Asian Lactifluus species are not conspecific with morphologically similar species present on other continents. In particular, Korea has misused European and North American Lactifluus names. In this study, we evaluated the taxonomy of Lactifluus in Korea using both morphological and multilocus molecular (ITS, nrLSU, rpb1, and rpb2) data. We examined 199 Lactifluus specimens collected between 1980 and 2016, and a total of 24 species across the four Lactifluus subgenera were identified. All Korean species are distinct and clearly separated from European and North American species. Five taxa corresponded to previously described species from Asia and the remaining 19 taxa are confirmed as new species. Herein, we provide keys to the Korean Lactifluus species within their subgenera, molecular phylogenies, a summary of diversity, and detailed description of the new species.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.326-331
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• 2021

The complete chloroplast genome of Zoysia macrostachya Franch. & Sav. isolated in Korea is 135,902 bp long (GC ratio is 38.4%) and has four subregions; 81,546 bp of large single-copy (36.3%) and 12,586 bp of small single-copy (32.7%) regions are separated by 20,885 bp of inverted repeat (44.1%) regions, including 130 genes (83 protein-coding genes, eight rRNAs, and 39 tRNAs). Thirty-nine single nucleotide polymorphisms and 11 insertions and deletion (INDEL) regions were identified from two Z. macrostachya chloroplast genomes, the smallest among other Zoysia species. Phylogenetic trees show that two Z. macrostachya chloroplast genomes are clustered into a single clade. However, we found some incongruency with regard to the phylogenetic position of the Z. macrostachya clade. Our chloroplast genome provides insights into intraspecific variations and species delimitation issues pertaining to the Zoysia species.

• ALGAE
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• v.32 no.1
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• pp.29-39
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• 2017

Geographic distributions of pathogens are affected by dynamic processes involving host susceptibility, availability and abundance. An oomycete, Pythium porphyrae, is the causative agent of red rot disease, which plagues Pyropia farms in Korea and Japan almost every year and causes serious economic damage. We isolated an oomycete pathogen infecting Pyropia plicata from a natural population in Wellington, New Zealand. The pathogen was identified as Pythium porphyrae using cytochrome oxidase subunit 1 and internal transcribed spacer of the rDNA cistron molecular markers. Susceptibility test showed that this Pythium from New Zealand was able to infect several different species and genera of Bangiales including Pyropia but is not able to infect their sporophytic (conchocelis) phases. The sequences of the isolated New Zealand strain were also identical to Pythium chondricola from Korea and the type strain from the Netherlands. Genetic species delimitation analyses found no support for separating P. porphyrae from P. chondricola, nor do we find morphological characters to distinguish them. We propose that Pythium chondricola be placed in synonymy with P. porphyrae. It appears that the pathogen of Pyropia, both in aquaculture in the northern hemisphere and in natural populations in the southern hemisphere is one species.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.188-195
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• 2001

The anatomical characteristics of rhizome, lateral root, stem, leaf, petiole, ovary and leaf epidermis about Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz. were investigated to estimate these taxonomic values. The cross sections of stem and ovary were very similar to two species, therefore these characters were not useful for delimitation of two species, but the size and distribution numbers per 100${\mu}{\textrm}{m}$$^2$of oil cavites in rhizome, the shape of leaf and petiole in cross section, the size of leaf epidermal cell and distribution numbers per 100 ${\mu}{\textrm}{m}$$^2$of stomata were differ from two species, therefore these were thought to be useful characters for delimitation of two species.

• Journal of Plant Biology
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• v.33 no.1
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• pp.1-24
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• 1990

This study was attempted to clarify the taxonomic problems that have been raised up to now in the study o section Telephium, genus, Sedum: the delimitation of taxa belong to Telephium group, their relationship, and nomenclatural confusion and the rank of Telephium group. Specimens were collected at 19 sites in Korea. The habitat, distribution, external morphological characters of habit, leaf shape, phyllotaxy, inflorescence, color of floral organs, floral formula, carpel, capsule, and micro-characters of pollen, seed coat, stigma and anther with SEM were examined. Through this study, the following results were obtained. The delimitation of taxa that belong to section Telephium in Korea and their relationship Nomenclatural errors were corrected. In S. rotundifolium Lee, species epithet“rotundifolium”is later homonym of s. rothundifolium Lamarck, so corrected to S. duckbongii Chung and Kim by article 64 of ICBN. Phyllotaxy was elucidated to meaningless, character in sect. Telephium. S. taquetii was a separate species from S. viridescens and not of its synonym. S. alboroseum Baker in Korea was S. erythrostictum sensu Master not of S. erythrostictum Miquel. By this study , taxa of sect. Telephium in Korea were arranged to 7 species. Considering interspecific relationship of sect. Telephium, S. duckbongii and S. alboroseum are considered to be more advanced forms and S. viridescens, S. taquetii, and S. viviparum to be more primitive forms among 7 species.

• Mycobiology
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• v.28 no.1
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• pp.39-46
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• 2000

Eight species of Marssonina parasitic on 21 species of host plants from Korea are described and illustrated. They are Marssonina brunnea (Ellis & Everh.) Magnus, M. capsulicola (Rostr.) Magnus, M. celastri H.D. Shin & H.T. Lee, M. coronaria (Ellis & Davis) Davis, M. fragariae (Lib.) Kleb., M. juglandis (Lib.) Magnus, M. rosae (Lib.) Died. and M. sennenis (Gonz. Frag.) Vassiljevsky & Karak. Of these, M. capsulicola and M. coronaria sometimes poss three-celled conidia and M. sennenis is characterized by a short appendage at the basal end of the conidia. Morphological features of conidia and host ranges were of taxonomic values for species delimitation.

• Korean Journal of Environmental Biology
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• v.23 no.2 s.58
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• pp.200-205
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• 2005

This study carried out to certify of taxonomic delimitation in A. artemisiaefolia, A. trifida, A. trifida for. integrifolia, and A. psilostachya in the area of literature and experiment. A. psilostachya was not a vestige of naturalized in Korea and A. trifida and A. trifida for. integrifolia didn't have any valuable differences of morphological and molecular biological experiment. I arranged that naturalized species in Ambrosia are A. artemisiaefolia L. and A. trifida L. in Korea.

• Animal cells and systems
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• v.2 no.4
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• pp.435-438
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• 1998

In the present study, we have examined the crossability of four species in the A. jaluense complex to provide additional information on their delimitation and levels of differentiation. Crosses between diploid A. chiisanense and the tetraploid species including A. jaluense s. str., A. triphyllum, and A. pseudoproliferum yielded no fruit- or seed-set, indicating that the former species is reproductively completely isolated from the latter three species. In interspecific crosses between the tetraploid species, combinations involving A. jaluense s. str. resulted in much reduced fruit- and seed-set, indicating that A. jaluense s. str. is more or less reproductively isolated from the other tetraploid species. However, crossing data revealed the absence of reproductive isolation between A. triphyllum and A. pseudoproliferum These results strongly support the relationships that have been previously suggested for the A. jaluense complex on the basis of morphology, chromosome number, and flavonoid chemistry.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.234-239
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• 2010

The taxonomy of umbel genus Sium L., Apiaceae in Korea was reviewed on the basis of molecular phylogenies derived from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) regions. The ITS sequence-derived phylogeny indicates that S. heterophyllum, endemic to Korea, is identical to S. tenue, which is known as endemic to Ussuri regions. Comparisons of sequence pairs across both spacer regions gave divergence values and revealed the identity between S. tenue and S. heterophyllum on Mt. Moonsoo. On the other hand, the ITS sequences support species delimitation of S. ternifolium, as reported recently as a new species that differs from other Sium species. The ITS sequence divergence values of 1.4 and 1.6% support species delimitation between S. serra and S. ternifolium.