• Journal of Microbiology
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• v.42 no.3
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• pp.248-252
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• 2004

In order to identify the protein(s) secreted into culture medium by the sool-l/retl-l mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28$^{\circ}C$) and non-permissive temperatures (37$^{\circ}C$), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37$^{\circ}C$. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37$^{\circ}C$, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the sool-l/retl-l mutation of S. cerevisiae.

• Communications of the Korean Mathematical Society
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• v.11 no.3
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• pp.673-680
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• 1996

It is proved that $K(c_0,Y)$ is an M-ideal in $L(c_0,Y)$ if Y is a closed subspace of $c_0$. And a new direct proof of the fact that $K(L_1[0,1],\ell_1)$ is not an M-ideal in $L(L_1[0,1],\ell_1)$ is given.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.42-48
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• 2001

Allele rescue and sequence analysis of soo1-1 allele in Saccharomyces cerevisiae mutant LP0353 revealed that soo1-1 is identical to the previously reported ret1-1 allele, which has a base substitution of A for $G^{681}$ leading to an amino acid substitution of aspartic acid for $glycine^{227}$ in Soolp. However, it was revealed that the addition of osmotic stabilizer, such as 1.2M sorbitol can rescue the temperature sensitive phenotype of the ret1-1 mutant and that the soo1-1/ret1-1 mutation may confer defects in post-translational modification of proteins involved in the yeast cell wall biogenesis. Evidence for a putative role of 5th WD40 domain of the Soo1p/$\alpha$-COP in the construction and maintenance of cell walls was also presented by complementation test with deletion constructs of the SOOl.

• Korean Journal of Food Science and Technology
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• v.53 no.5
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• pp.640-647
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• 2021

In this study, we analyzed three rice pre-treatment methods for the preparation of mit-sool (first mashing), with godubap (steamed rice), beombeok (undercooked porridge), and juk (porridge). The results can provide basic data for the development of takju with improved quality. In terms of the base alcohol, godubap produced the highest amount of alcohol (8.1%) after two days, followed by beombeok and juk. After two days of fermentation, soluble solids were measured at 15.3, 15.1, and 1.4°Brix in beombeok, juk, and godubap, respectively, The glucose content of godubap did not change significantly; however, the maltose content increased. In beombeok, maltose decreased from 16.48 mg/mL on the initial day 0 to 1.27 mg/mL on day 9. In juk, glucose and maltose increased from 14.05 and 11.49 mg/mL on day 0 to 31.39 and 42.53 mg/mL on day 2, respectively. Succinic acid levels increased in the godubap and beombeok mit-sool. However, succinic acid was not detected in godubap and juk during the initial stage of fermentation, and lactic acid was not detected in beombeok mit-sool. During the fermentation stage of deot-sool (second mashing), the highest alcohol content of takju made with godubap alcohol was 18.0% and the lowest alcohol content was 15.1% in beombeok on day 9. The total acidity was as high as 0.54% for beombeok, 0.41% for juk, and 0.39% for godubap. In the flavor analysis, ethyl caprate and ethyl caprylate, which were high on day 0 in godubap, decreased upon the completion of fermentation on day 9. Banana-flavored isoamyl alcohol content increased. For juk, ethyl caprate and ethyl caprylate, which were high on day 0, decreased on day 9. Flavor components, such as ethyl palmitate and ethyl linoleate were increased.

• Bulletin of the Korean Mathematical Society
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• v.35 no.4
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• pp.683-687
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• 1998

Suppose X is a subspace of $(\sum_{n=1} ^{\infty} X_n)_{c_0}$, dim $X_n<{\infty}$, which has the metric compact approximation property. It is proved that if Y is a Banach space of cotype q for some $2{\leq}1<{\infty}$ then K(X,Y) is an M-ideal in L(X,Y).

• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.195-205
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• 1992

The soluble antigen profiles and antigenic specificities of Leptospira interrogans serovars icterhaemorrhagiae, canicola, pomona and hardjo were examined by SDS-polyacrylamide gel electrophoresis, crossed immunoeletrophoresis and immunoblotting. The profiles of protein, glycoprotein and fraction containing N-acetylglucosamine of 4 serovars were compared. The protein profiles of 4 serovars were very similar except the range of 14,400 to 30,000 daltons. Molecular weight of glycoprotein of L, pomona was lower than other serovars. L canicola showed extra N-acetylglucosamine bands having molecular weight of 82,000 and 90,000 daltons. In crossed immunoelectrophoresis, a close antigenic relationship was found between L icterohaemorrhagiae and L canicola. In immunoblottings conducted with soluble antigens and rabbit antisera of 4 serovars, Leptospira interrogans serovars possessed cross-reactive antigens and serovar-specific antigens. The molecular weights of serovar-specific antigens were 45,000, 82,000 and 90,000, 31,000 and 24,000 daltons in L icterohaemorrhagiae, L canicola, L pomona, and L hardjo, respectively.

• Journal of the Korean Applied Science and Technology
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• v.27 no.2
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• pp.183-187
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• 2010

Core-Shell polymers of silicone dioxide-styrene system were prepared by sequential emulsion polymerization. In inorganic/organic Core-Shell composite particle polymerization, silicone dioxide adsorbed by surfactant sodium dioctyl sulfosuccinate (EU-DO133L) was prepared initially and then core silicone dioxide was encapsulated emulsion by sequential emulsion polymerization using styrene at the addition of potassium persulfate (KPS) as an initiator. We found that $SiO_2$ core shell of $SiO_2$/styrene structure was formed when polymerization of styrene was conducted on the surface of $SiO_2$ particles, and the concentration sodium dioctyl sulfosuccinate (EU-DO133L) was 0.5~2.0g. The structure of core-shell polymer were investigated by measuring to the thermal decomposition of polymer composite using thermogravimetric analyzer and morphology of latex by scanning electron microscope(SEM).

• Animal Systematics, Evolution and Diversity
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• v.25 no.3
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• pp.301-308
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• 2009

Three Loxodes ciliates collected from estuarine littoral, wetland and small pond in Korea, were identified as Loxodes kahli Dragesco and Njin$acute{e}$, 1971, L. magnus Stokes, 1887 and L. vorax Stokes, 1885. The descriptions for these species based on living and protargol impregnated specimens were given. Morphometry, illustrations and microphotographs were also provided. Diagnoses of three species are as follows. Loxodes kahli; size in vivo $160-300{\times}40-70\;{\mu}m$; oral area with reddish to brownish pigments; 6-11 macronuclei arranged linearly; 5-9 micronuclei located near macronuclei; 4-12 M$\ddot{u}$ller's vesicles; somatic kineties on right 18-20 and left 2 in number. L. magnus: size in vivo $250-470{\times}87-15\;{\mu}m$; body colored dark brown; 5-13 macronuclei; 8-13 micronuclei; 8-18 M$\ddot{u}$ller's vesicles; somatic kineties on right 23-26 and left 2 in number. L. vorax: size in vivo $70-160{\times}20-35\;{\mu}m$; oral area with brownish pigments; 2 macronuclei; 1 micronucleus located between macronuclei; 2-4 M$\ddot{u}$ller's vesicles; somatic kineties on right 18-20 and left 2 in number.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.357-360
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• 1997

Multiple shoot formation was obtained from excised axillary buds of Achyranthes japonica NAKAI cultured on MS media containing various growth regulators such as auxin and cytokinin. The highest average number of shoots was obtained in 1 mg/L NAA and 2 mg/L BA after 6 weeks (25.8 adventitious shoots per node). Although the regeneration rate was less than the former condition, optimal combination for the production of more shoots with a suitable size was 0.5 mg/L NAA and 1 mg/L BA (19.7 adventitious shoots per node). Roots were induced from regenerated shoots after 3 weeks culture, transferred to 1/2 MS medium supplemented with 0.1 mg/L IBA. Micropropagated plants were successfully transferred to soil.

• Journal of the Korean Applied Science and Technology
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• v.27 no.2
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• pp.202-207
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• 2010

We investigated the electrochemical properties for Langmuir-Blodgett(LB) films mixed with l-bromotetradecane(Cl4), l-bromohexadecane(Cl6), and l-bromooctadecane(Cl8). The alkyl bromides mixture was deposited by using the Langmuir-Blodgett method on the ITO glass. The electrochemical properties measured by using cyclic voltammetry with a three-electrode system(an Ag/AgCl reference electrode, a platinum wire counter electrode and LB film-coated ITO working electrode) at various concentrations(0.5, 1.0, 1.5 and 2.0 N) of $NaClO_4$ solution. A measuring range was reduced from initial potential to -1350 m V, continuously oxidized to 1650 mV. The scan rate was 100 mV/s. As a result, LB films of Cl4, Cl6, and Cl8 mixture monolayers appeared irreversible process caused by only the oxidation current from the cyclic voltammogram. The diffusivity(D) effect of LB films decreased with increasing of alkyl bromides amount.