• Bulletin of the Korean Chemical Society
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• v.7 no.5
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• pp.399-402
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• 1986

The critical micelle concentration (CMC) of sodium deoxycholate (NaDC) and the effects of amides on the micellization processes have been studied by fluorometric technique using pyrene as a probe. The addition of amides as cosolvent destabilized the NaDC micelle and increased the CMC. The order of effectiveness for the perturbation of NaDC micelle was N-methylacetamide ${\ge}$ DMF > acetamide > formamide, which is the order of hydrophobicity of the amines. This indicated that the effect of amides on the micellization processes of NaDC arises from diminution of the hydrophobic effect. The electrostatic repulsion between ionic head groups in the NaDC micelle appeared to be much less than that in aliphatic ionic micelle. This was also revealed in the weaker dependence of the CMC on ionic strength. The premicellar association of NaDC was not significantly involved in the micellization processes of the bile salt.

• YAKHAK HOEJI
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• v.28 no.3
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• pp.161-167
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• 1984

To improve the solubility properties of indomethacin, a poorly water-soluble drug, some attempts were made with surfactants, such as sodium deoxycholate, sodium laurylsulfate and polysorbate-80. The enhancement effect in solubility was found to be due to the micellar solubilization and dependent on the concentration of surfactants, pH and temperature. The mechanism of interaction was suggested to be attributed to the formation of mixed micelles between the drug and solubilizers.

• Journal of Chest Surgery
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• v.41 no.5
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• pp.550-562
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• 2008

Background: We attempted to reproduce a previously reported method that is known to be effective for decellularization, and we sought to find the optimal condition for decellularization by introducing some modifications to this method. Material and Method: Porcine semilunar valves, arterial walls and pericardium were processed for decellularization with using a variety of combinations and concentrations of decellularizing agents under different conditions of temperature, osmolarity and incubation time. The degree of decellularization and the preservation of the extracellular matrix were evaluated by staining with hematoxylin and eosin and with alpha-Gal and DAPI in some of the decellularized tissues. Result: Decellularization was achieved in the specimens that were treated with sodium deoxycholate, sodium dodesyl sulfate, Triton X-100 and sodium dodesyl sulfate with Triton X-100 as single-step methods, and this was also achieved in the specimens that were treated with hypotonic solution ${\rightarrow}$ Triton X-100 ${\rightarrow}$ sodium dodesyl sulfate, sodium deoxycholate ${\rightarrow}$ hypotonic solution ${\rightarrow}$ sodium dodesyl sulfate, and hypotonic solution sodium dodesyl sulfate as multi-step methods. Conclusion: Considering the number and the amount of the chemicals that were used, the incubation time and the degree of damage to the extracellular matrix, a single-step method with sodium dodesyl sulfate and Triton X-100 and a multi-step method with hypotonic solution followed by sodium dodesyl sulfate were both relatively optimal methods for decellularization in this study.

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.163-170
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• 1993

The effect of substrate micellization on the hydrolysis rate in the production of lysopho-sphatidylcholine (LPC) from phosphatidylcholine (PC) using hog pancreas phospholipase A2(PLA2) was studied. The optimal temperature and pH for the reactions in aqueous phase was found 42C and 7.2, respectively. For a given PC concentration, initial reaction rate was progressively increased with the addition of sodium deoxycholate (DOC), which could transform the bilayer of phospholipids into micellar structure.

• Bulletin of the Korean Chemical Society
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• v.8 no.2
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• pp.118-122
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• 1987

The micelle formation of NaDC was studied by fluorometric and viscometric measurements. The thermodynamic parameters of the primary and secondary micellization of the bile salt were evaluated. The primary micelle formation was appeared to be an entropy driven process due to hydrophobic effect, while the major driving force for secondary micelle formation of the bile salt is the large negative enthalpy. The secondary micelle provides less hydrophobic environment to pyrene than the primary micelle does. The cooperative aggregation of primary micelles via hvdrogen bond formation was proposed for the secondary micelle formation.

• Applied Chemistry for Engineering
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• v.31 no.5
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• pp.545-551
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• 2020

An attempt was made to prepare hydrated liquid crystalline vesicles by utilizing a mutual self-assembly between phospholipid and ceramide. When an edge activator was added to the mixture of phospholipid and ceramide, it was possible to prepare a vesicle containing a high content of poorly soluble ceramide. In this study, we investigated changes in structural and thermal behaviors of the hydrated liquid crystalline phase according to the mixed composition between phospholipid, ceramide, and sodium deoxycholate. Also, both the droplet size of the vesicles with different composition and stability of the vesicle dispersion solution were observed. From results of the experiments, by increasing the ratio of ceramide up to 70% in the mixture between phospholipid and ceramide, a formulation containing 3.5 wt% of ceramide in the vesicle dispersion solution could be obtained.

• Applied Chemistry for Engineering
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• v.32 no.5
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• pp.541-546
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• 2021

Hydrated liquid crystalline vesicles containing a high content of ceramide were prepared by constituting an optimal composition in which ceramides can be mutually self-associated with phospholipid and cholesterol. From the result of manufacturing various vesicles with different component composition, when the edge activator sodium deoxycholate (SDOC) and the solubilizer PEG-60 hydrogenated castor oil (HCO 60) were mixed to form vesicles, the smallest nano-sized particles were produced and the vesicle dispersion solution was weakly acidic and maintained the most stable state. In addition, it was confirmed through polarized light microscopy and thermal analysis that the addition of SDOC and HCO 60 had an effect on the inhibition of crystallinity of lipid components such as ceramide. The stability of the vesicle dispersion solution was maintained without change in appearance and viscosity even after long-term storage at high temperature for eight weeks.

• Journal of the Korean Applied Science and Technology
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• v.41 no.2
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• pp.318-328
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• 2024

In this study, we enhanced the emulsifying power and solubilization of sucrose stearate (SS) by creating mixtures with sodium deoxycholate (SDOC) and PEG-40 hydrogenated castor oil (HCO). We employed the design of mixture experiments (DOME) methodology to identify the optimal composition of the mixture, and the impact of varying the mixture composition on its characteristics was examined through regression analysis of the experimental data. It was revealed that the emulsifying power for coconut oil was most improved when only SDOC was added to SS, and solubilization was most improved when only HCO was added, while the emulsifying power for cetyl ethylhexanoate (CEH) was most significantly improved when SDOC and HCO were added together. As a result of simultaneous optimization of three characteristics, emulsifying power for each of coconut oil and CEH, and solubilization, the optimal surfactant mixture composition was determined as SS 0.7939, SDOC 0.0586, and HCO 0.1475.

• Microbiology and Biotechnology Letters
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• v.21 no.5
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• pp.414-420
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• 1993

Escherichia coli mrdAts and mrdBts mutants forming spherical cells at 42C, were employed to investigate the possible role of both inner and outer membrance structures in the determination of cell shape of gram-negative cells. Spherical cells, but not rod-shaped wild types, were specifically killed by anionic detergents, such as sarkosyl, sodium dodecylsulfate and sodium deoxycholate. From the spherical intact cells grown overnight at 42C, much more proteins were released by sakosyl.

• Journal of Pharmaceutical Investigation
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• v.33 no.3
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• pp.187-193
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• 2003

This study was aimed to formulate various phospholipid nanoparticles composed of different surfactants and to evaluate the deformability of the phospholipid vesicles as candidates of useful ultradeformable nanoparticles. In vitro deformability of the phospholipid nanoparticles was studied using an extruder under a certain pressure. The sizes of phospholipid nanoparticles, passed volumes, and concentrations of the phospholipids in suspensions before and after extrusion were measured. The deformability indexes were estimated by using passed volumes, sizes of phospholipid nanoparticles and concentrations of phospholipids. Conventional liposomes, placed under a certain pressure of an extruder, showed no passed volume indicating little deformability. Similar to conventional liposomes, phospholipid nanoparticles containing surfactants such as sodium taurocholate, Myrj 45, or Myrj 53 showed little deformability. In contrast, phospholipid nanoparticles composed of Tween 20, Triton X-100, or sodium deoxycholate showed higher deformability indexes than others. Taken together, the deformability of phospholpid nanoparticles could be significantly affected by the type of surfactants. Moreover, these results suggest that the deformability of phospholipid nanoparticles could be modulated by surfactants.