• Title/Summary/Keyword: slide

Search Result 982, Processing Time 0.024 seconds

Development of Microarrayer for DNA Chips (DNA Chip 제작을 위한 Microarrayer의 개발)

  • Kim, Suk-Yoel;Jung, Nam-Su;Im, Jae-Sung;Kim, Sang-Bong
    • Proceedings of the KSME Conference
    • /
    • 2003.04a
    • /
    • pp.899-904
    • /
    • 2003
  • Microarrayer makes DNA chip and microarray that contain hundreds to thousands of immobilized DNA probes on surface of a microscope slide. This paper shows the development results for a printing type of microarrayer. It realizes a typical, low-cost and efficient microarrayer for generating low density microarray. The microarrayer is developed by using a robot of three-axes perpendicular type. It is composed of a computer-controlled three-axes robot and a pen tip assembly. The key component of the arrayer is the print-head containing the tips to immobilize cDNA, genomic DNA or similar biological material on glass surface. The robot is designed to automatically collect probes from two 96-well plates with up to 32 tips at the same time. To prove the performance of the developed microarrayer, the general water types of inks such as black, blue and red. The inks are distributed at proper positions of 96 well plates and the three color inks are immobilized on the slide glass under the operation procedure. As the result of the test, it can be shown that it has sufficient performance for the production of low integrated DNA chip consisted of 96 spots within 1 $cm^2$ area.

  • PDF

Synthesis of an On-Line 5 Degrees of Freedom Error Measurement System for Translational Motion Rigid Bodies (병진운동 강체의 온라인 5자유도 운동오차 측정시스템 설계 및 해석)

  • 김진상;정성종
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.15 no.5
    • /
    • pp.93-99
    • /
    • 1998
  • Although laser interferometer measurement system has advantages of measurement range and accuracy, it has some disadvantages when measurement of multi degrees of freedom of motion are required. Because the traditional error measurement methods for geometric errors (two straightness and three angular errors) of a slide of machine tools measures error components one at a time. It may also create an optical path difference and affect the measurement accuracy. In order to identify and compensate for geometric errors of a moving rigid body in real time processes, an on-line error measurement system for simultaneous detection of the five error components of a moving object is required. Using laser alignment technique and some optoelectronic components, an on-line measurement system with 5 degrees of freedom was developed for the geometric error detection in this study Performance verification of the system has been performed on an error generating mechanism. Experimental results show the feasibility of this system for identifying geometric errors of a slide of machine tools.

  • PDF

Investigation of the Thermal Mode-based Thermal Error Prediction for the Multi-heat Sources Model (다중열원모델의 열모드기반 열변위오차 예측)

  • Han, Jun An;Kim, Gyu Ha;Lee, Sun-Kyu
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.30 no.7
    • /
    • pp.754-761
    • /
    • 2013
  • Thermal displacement is an important issue in machine tool systems. During the last several decades, thermal error compensation technology has significantly reduced thermal distortion error; this success has been attributed to the development of a precise, robust thermal error model. A major advantage of using the thermal error model is instant compensation for the control variables during the modeling process. However, successful application of thermal error modeling requires correct determination of the temperature sensor placement. In this paper, a procedure for predicting thermal-mode-based thermal error is introduced. Based on this thermal analysis, temperature sensors were positioned for multiple heat-source models. The performance of the sensors based on thermal-mode error analysis, was compared with conventional methods through simulation and experiments, for the case of a slide table in a transient state. Our results show that for predicting thermal error the proposed thermal model is more accurate than the conventional model.

A Study on the Feed Rate Optimization of a Linear Motored Feed Drive System for Minimum Vibrations (Linear Motor 이송계의 진동 최소화를 위한 이송속도 최적화)

  • 최영휴;홍진현;최응영;김태형;최원선
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
    • /
    • 2004.10a
    • /
    • pp.321-325
    • /
    • 2004
  • Linear motor feed drive systems have been broadly used in machine tools or precision automatic feed systems. Recently, modem machine tools require high speed and high precision feed drive system to achieve high productivity. Unfortunately, a feed drive system, even though it was optimum designed, may experience severe transient vibrations during high-speed operation if its feed rate control is unsuitable. A rough feed rate curve having discontinuity in its acceleration profile causes a serious vibration problem in the feed slides system. This paper presents a feed rate optimization of a machine tool feed slide system, which is driven by a linear motor, for its minimum vibrations. Firstly, a 4-degree-of-freedom lumped parameter model is proposed for the vibration analysis of a linear motor driven machine tool feed drive system. Next, a feed rate optimization of the feed slide is carried out for minimum vibrations. The feed rate curve optimization strategy is to find out the most appropriate acceleration profile with jerk continuity. Of course, the optimized feed rate should approximate to the desired one as possible. A genetic algorithm with variable penalty function was used in this feed rate optimization.

  • PDF

Calibration of Parallel Manipulators using a New Measurement Device (새로운 측정장비를 이용한 병렬구조 로봇의 보정에 관한)

  • Rauf, Abdul;Kim, Sung-Gaun;Ryu, Je-Ha
    • Proceedings of the KSME Conference
    • /
    • 2003.11a
    • /
    • pp.1494-1499
    • /
    • 2003
  • Kinematic calibration is a process whereby the actual values of geometric parameters are estimated so as to minimize the error in absolute positioning. Measuring all components of Cartesian posture, particularly the orientation, can be difficult. With partial pose measurements, all parameters may not be identifiable. This paper proposes a new device that can be used to identify all kinematic parameters with partial pose measurements. Study is performed for a six degree-of-freedom fully parallel Hexa Slide manipulator. The device, however, is general and can be used for other parallel manipulators. The proposed device consists of a link with U joints on both sides and is equipped with a rotary sensor and a biaxial inclinometer. When attached between the base and the mobile platform, the device restricts the end-effector's motion to five degree-of-freedom and can measure position of the end-effector and one of its rotations. Numerical analyses of the identification Jacobian reveal that all parameters are identifiable. Computer simulations show that the identification is robust for the errors in the initial guess and the measurement noise.

  • PDF

Discrimination of geographical origin and cultivation years of Ginseng by near Infrared reflectance spectroscopy

  • Lin, Guo-Lin;Sohn, Mi-Ryeong;Cho, Rae-Kwnag;Hong, Jin-Hwan
    • Near Infrared Analysis
    • /
    • v.1 no.2
    • /
    • pp.41-44
    • /
    • 2000
  • The objectives of this study are to discriminate the geographical origin and cultivation years of ginseng based on the near-infrared(NIR) reflectance spectroscopic analysis. Korea and China ginseng samples were prepared for discrimination of geographical origin. 4, 5 and 6 years-old ginseng samples from Korea were prepared for discrimination of cultivation years. Used spectrometer were InfraAlyzer 500, InfraAlyzer 400 and Fiber optic. Sample type of ginseng was 3, whole ginseng radix, slide section and powder type. The accuracy was affected by sample types and instruments. The accuracy for discrimination geographical origin was 97% in calibration model using IA 500 and ginseng powder. For discrimination of cultivation years, the model with slide selection using IA500 were relative accurate. The accuracy was 96.7% for 4-year, 91.3% for 5-year and 89.3% for 6-year old ginseng. The study shows that NIR spectroscopic analysis can be used to discriminate the geographical origin and cultivation years of ginseng with acceptable accuracy.

Effect of Shear Stress on Bovine Aortic Smooth Muscle Cell Growth (우 대동맥 평활근 세포의 성장에 관한 shear stress의 영향)

  • 김동욱
    • KSBB Journal
    • /
    • v.11 no.1
    • /
    • pp.52-57
    • /
    • 1996
  • Bovine aortic smooth muscle cells cultured on the slide glass were exposed to sheared flow up to 120 hours in flow chamber to see the effect of shear stress on cell growth in wall shear stresses of 0 to 26dyn/$cm^2$. From lactate dehydrogenase concentration measurement of the circulating medium, it was shown that sheared flow in the shear stress range did not remove additional smooth muscle cells from the slide glass compared with cells in stationary condition. According to smooth muscle cell counting per$cm^2$ of the surface, smooth muscle cells grew fastest in the stationary condition. As the wall shear stress increased, the growth of cells became slower. When the wall shear stress increased over 17dyn/$cm^2$, cell growth was not observed throughout the experiment.

  • PDF

Design of Hydraulic & Control System for the Disc Spinning Machine (디스크 스피닝 성형기의 유압 및 제어시스템 설계)

  • Gang, Jung-Sik;Park, Geun-Seok;Gang, E-Sok
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.19 no.9
    • /
    • pp.157-165
    • /
    • 2002
  • The design of hydraulic & control system has been developed for the disc spinning machine. The hydraulic system has been designed in the overall system including the vertical & horizontal slide fur spinning works which are controlled by hydraulic servo valves in right & left side, and the clamping slide for holding & pressing blank material in center during spinning process. Based on the design concept of this hydraulic system, model test experiments for hydraulic servo control system is tested to conform confidence and applying possibility. The control system is introduced with the fuzzy-sliding mode controller for the hydraulic force control reacting force as a disturbance, because a fuzzy controller does not require an accurate mathematical model for the generation of nonlinear factors in the actual nonlinear plant with unknown disturbances and a sliding controller has the robustness & stability in mathematical control algorithm. We conform that the fuzzy-sliding mode controller has a good performance in force control for the plant with a strong disturbance. Also, we observe that a steady state error of the fuzzy-sliding mode controller can be reduced better than those of an another controllers.

Numerical Analysis on the High Speed Precision Press for Ultra-thin Sheet Metal Forming (초박판 성형용 고속 정밀프레스에 대한 해석적 연구)

  • Kang, J.J.;Kim, J.E.;Hong, S.K.;Kim, J.D.;Heo, Y.M.;Cho, C.
    • Transactions of Materials Processing
    • /
    • v.17 no.8
    • /
    • pp.643-648
    • /
    • 2008
  • Ultra-thin sheet metal forming techniques are required in precision forming of miniaturized and integrated products. In order to manufacture a good quality and low cost ultra-thin sheet metal products, a highly precise high-speed press is needed. The precision of a press is related with its vibration characteristics during pressing operation. This study evaluated the vibration characteristics of a proposed press design using computer simulation. The analysis compares the static deformation characteristics of the slide and the slide motion for the metal forming of an ultra-thin sheet of thickness less than 0.1mm. Further, in order to minimize the vibrations during high speed pressing operation, revolution balances of the eccentric shaft and the balance weight device is also considered. Finally, modal analysis is used to characterize the natural frequency of vibration of the press.

An Investigation on the Undentanding of Spatial Sense of Elementary School Students (초등학생들의 공간감각 이해능력 실태조사)

  • Lee, Sung-Mi;Pang, Jeong-Suk
    • The Mathematical Education
    • /
    • v.46 no.3
    • /
    • pp.273-292
    • /
    • 2007
  • The purpose of this study was to find out how second, fourth and sixth graders understood the main contents related to spatial sense in the Seventh National Mathematics Curriculum. For this purpose, this study examined students' understanding of the main contents of congruence transformation (slide, flip, turn), mirror symmetry, cubes, congruence and symmetry. An investigation was conducted and the subjects included 483 students. The main results are as follows. First, with regards to congruence transformation, whereas students had high percentages of correct answers on questions concerning slide, they had lower percentages on questions concerning turn. Percentages of correct answers on flip questions had significant differences among the three grades. In addition, most students experienced difficulties in describing the changes of shapes. Second, students understood the fact that the right and the left of an image in a mirror are exchanged, but they had poor overall understanding of mirror symmetry. The more complicated the cubes, the lower percentages of correct answers. Third, students had a good understanding of congruences, but they had difficulties in finding out congruent figures. Lastly, they had a poor understanding of symmetry and, in particular, didn't distinguish a symmetric figure of a line from a symmetric figure of a point.

  • PDF