• Journal of Plant Biotechnology
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• 제38권1호
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• pp.42-48
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• 2011

고등식물의 엽록체 유전공학은 핵 형질전환과 비교해 볼때 여러 가지 독특한 장점을 가진다. 높은 transgene 발현율, 상동 재조합에 의한 site-specific transgene의 삽입으로 인해 유전자의 position effect가 없으며, 단일 형질전환으로 동시에 여러 유전자의 도입이 가능하고 모계 유전으로 인해 화분 방출 위험을 감소시킬 수 있다. 담배 specific한 pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA) 벡터를 이용하여 안정적인 감자 엽록체 형질전환 시스템을 개발하였다. 감자 엽록체 게놈으로 외래유전자의 삽입과 homoplasmic level은 PCR과 Southern blot 분석으로 확인하였다. Northern과 immunoblot 분석 및 GFP fluorescence imaging을 통하여 엽록체 형질전환체의 잎에서 GFP 유전자가 강하게 발현, 축적됨을 알 수 있었다. 본 연구에서 확립된 감자 엽록체 형질전환 시스템을 이용하여 유용 유전자를 도입함으로써 농업적 형질을 개선하거나 고부가가치 단백질을 대량 생산하는 감자를 보다 효율적으로 개발할 수 있을 것이다.

• Molecules and Cells
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• 제38권11호
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• pp.950-958
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• 2015

BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.

• Molecules and Cells
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• 제40권2호
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• pp.100-108
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• 2017

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by producing a conserved 3' splice site. Six transcript variants (V1-V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5' splice sites in the 5' and 3' flanking regions of CTSF_AluYRa1. Among them, V3-V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

• 한국조경학회지
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• 제35권4호
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• pp.32-39
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• 2007

Side roads on campus areone of the important factors affecting campus landscape and quality. So far, side roads have appeared irregularly and have been main causes for disrupting the campus landscape. The purpose of this study was to investigate side roads in three university campuses using Space Syntax and ERAM model and to find some typical characteristics of the campus in three universities: Kyungpook National University, Yeungnam University, and Keimyung University. Kyungpook National University and Yeungnam University had 68 and 64 places respectively showing a high frequency of side roads, while Keimyung University had only 13 side roads, a much lower frequency. Side roads, surveyed on site, appeared to be concentrated in some regions, and side roads were analyzed to have different frequencies and location depending on their specific function and characteristics, though roads have similar sizes and areas.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 B
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• pp.653-656
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• 2001

This paper describes the design and the analysis of permanent magnetic actuator "Magnetic Switch" using classical method and 2D finite element method. The classical method gives an outline of permanent magnet size and analysis is carried out by finite element method. Therefore we make use of the result in specific detail site of magnet. The transient state is simulated in order to calculate the response time of "Magnetic Switch". The simulation is based upon a step-by-step integration of the electric circuit equations and the tore movement. The contactor uses a permanent magnet for maintaining the closed state. The presented solution takes account of non-linear magnetic material property and spring force controlled by core position. The dynamic response of "Magnetic Switch" is predicted by the simulation agrees closely with the required condition.

• 한국세라믹학회지
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• 제55권2호
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• pp.95-107
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• 2018

This presentation will give an overview of Ca-aluminate based biomaterials and their proposed use within the field of nanostructured biomaterials. The paper describes typical features of Ca-aluminate materials with regard to technology, chemistry, biocompatibility including hemocompatibility and bioactivity, and developed microstructure. Special focus will be on the developed microstructure, which is in the nanosize range. Application possibilities within odontology, orthopedics, and drug delivery are presented. The nanostructure including pore size below 5 nm in these structures opens up this material for some use in specific dental-related applications in which antibacterial and bacteriostatic aspects are of importance, and as thin coating on implants within dental and orthopaedic applications. Nanosize porosity is essential in drug delivery systems for controlled release of medicaments. The priority field for Ca-aluminate biomaterials is implant materials, which use minimally-invasive techniques to offer in vivo, on-site developed biomaterials.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1996년도 춘계학술발표회논문집(2)
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• pp.241-246
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• 1996

An analytical model to calculate rate of vapor generation due to heterogeneous wall nucleation in flashing flow is developed. In the present model, an important parameter of the vapor generation term, i.e. nucleation site density is calculated by integrating its probability distribution function with respect to active cavity radius. The limits of integration are minimum and maximum active cavity radii, and these are formulated using an active cavity model for nucleate boiling. This formulation, therefore. can statistically account for the effect of surface specific thermo-physical and geometric conditions on the vapor generation rate and flashing inception. For verifying the adequacy of the present model, steady state two-fluid and the bubble transport equations are solved with applicable constitutive equations. The applicable region of the bubble transport equation is also extended to churn-turbulent flow regime to predict interfacial area concentration at high void fraction. Predicted results in terms of axial pressure and void fraction profiles along the channels are compared with experimental data of Super Moby Dick and BNL Reasonable agreements have been achieved and this shows the applicability of the present model to flashing flow analysis.

• BMB Reports
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• 제43권12호
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• pp.781-788
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• 2010

An often overlooked issue in the field of adenovirus (Ad)-mediated cancer gene therapy is its limited capacity for effective systemic delivery. Although primary tumors can be treated effectively with intralesional injection of conventional Ad vectors, systemic metastasis is difficult to cure. Systemic administration of conventional naked Ads leads to acute accumulation of Ad particles in the liver, induction of neutralizing antibody, short blood circulation half-life, non-specific biodistribution in undesired organs, and low selective accumulation in the target disease site. Versatile strategies involving the modification of viral surfaces with polymers and nanomaterials have been designed for the purpose of maximizing Ad anti-tumor activity and specificity by systemic administration. Integration of viral and non-viral nanomaterials will substantially advance both fields, creating new concepts in gene therapeutics. This review focuses on current advances in the development of smart Ad hybrid nanocomplexes based on various design-based strategies for optimal Ad systemic administration.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.274-276
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• 2003

The traditional crop productivity simulations based on crop models are normally site-specific. To simulate regional crop productivity, the spatial crop model is developed in this study by integrating Geographical Information System (GIS) with Erosion Productivity Impact Calculator (EPIC) model. The integration applied a loose coupling approach. Data are exchanged using ASCII or binary data format between GIS and EPIC model without a common user interface. The spatial EPIC model is conducted to simulate the average corn and wheat productivity of 1980s in North China. The results show that the simulation accuracy of the spatial EPIC model is acceptable. The simulation accuracy can be improved by using the detailed crop management information, such as irrigation, fertilizer and tillage schedule.

• 한국BIM학회 논문집
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• 제14권1호
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• pp.11-21
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• 2024

This study is about a case of web application development using representative commercial BIM platform-linked APIs from Autodesk and Bentley Systems. The development method was aimed at application development using Autodesk's Forge API and Bentley System's iTwin API. The specific development content was limited to the Model Viewer, Issues, and File Transfer API, and the administrator's site. The first research result is that parts with different internal configurations of commercial platforms were independently modularized and developed, and the common front-end was unified. The second research result is that a web front-end technology, was used to visualize 3D models using the viewer API, and the possibility of additional application expansion was discovered.