• The KIPS Transactions:PartC
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• v.14C no.7
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• pp.559-570
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• 2007

In this paper, we define a Hose-NSIS-QoSM, which reserves and manages resources according to the hose based resource provisioning mechanisms for supporting the VPN QoS(Quality of Service) using the NSIS(Next Step in Signaling) signaling protocol. Specifically, we specify the NSIS message objects, the structures of QoS NSLP(NSIS Signaling Layer Protocol)/NTLP(NSIS Transport Layer Protocol)/RMF(Resource Management Function) state tables and the processing of the signaling messages in NSIS nodes. Also, we compare the Hose-NSIS-QoSM with the Hose-RSVP-TE-QoSM that supports the hose based VPN QoS in the MPLS networks using the extended RSVP-TE mechanism.

• Herbal Formula Science
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• v.31 no.1
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• pp.41-51
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• 2023

Objectives : Raphani Semen (Raphanus sativus L.) is known for the various beneficial effects in Korean medicine. This study aimed to investigate the effect of Raphani Semen extract (RSE) against arachidonic acid (AA)+iron-induced oxidative stress in cells. Methods : Ingredients, their target information, oxidative stress liver injury-related proteins was obtained from various network pharmacology databases and software. A hypergeometric test and enrichment analysis were conducted to evaluate associations between protein targets of RSE. The cell viability was assessed by MTT assay, and immunoblot analysis was used to confirm the molecular mechanisms. Results : A compound-target network of RSE was constructed, which consisted of 336 edges between 18 ingredients and 123 protein targets. PI3K-Akt signaling pathway, ErbB signaling pathway, HIF-1 signaling pathway, PPAR signaling pathway, and AMPK signaling pathway was significantly associated with protein targets of RSE. RSE protected HepG2 cells against AA+iron-induced oxidative stress as mediated with AMPK signaling. Conclusion : RSE was found to protect the cells against oxidative stress via the AMPK signaling pathway.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.3
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• pp.139-144
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• 2010

In this study, we evaluated the role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the tumor necrosis factor-$\alpha$ (TNF-$\alpha$) induced cyclooxygenase-2 (COX-2) expression using A549 lung adenocarcinoma cells. TNF-$\alpha$ induced the expression of COX-2 in A549 cells, but did not induce BEAS-2B expression. The expression of COX-2 in A549 cells was TNF-$\alpha$ dose-dependent (5~100 ng/ml). TNF-$\alpha$-stimulated A549 cells evidenced increased Ref-1 expression in a dose-dependent manner. The adenoviral transfection of cells with AdRef-1 inhibited TNF-$\alpha$-induced COX-2 expression relative to that seen in the control cells ($Ad{\beta}gal$). Pretreatment with $10\;{\mu}M$ of SB203580 suppressed TNF-$\alpha$-induced COX-2 expression, thereby suggesting that p38 MAPK might be involved in COX-2 expression in A549 cells. The phosphorylation of p38 MAPK was increased significantly after 5 minutes of treatment with TNF-$\alpha$, reaching a maximum level at 10 min which persisted for up to 60 min. However, p38MAPK phosphorylation was markedly suppressed in the Ref-1-overexpressed A549 cells. Taken together, our results appear to indicate that Ref-1 negatively regulates COX-2 expression in response to cytokine stimulation via the inhibition of p38 MAPK phosphorylation. In the lung cancer cell lines, Ref-1 may be involved as an important negative regulator of inflammatory gene expression.

• Molecules and Cells
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• v.38 no.12
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• pp.1064-1070
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• 2015

Translocator protein 18 kDa (TSPO) is a mitochondrial outer membrane protein and is abundantly expressed in a variety of organ and tissues. To date, the functional role of TSPO on vascular endothelial cell activation has yet to be fully elucidated. In the present study, the phorbol 12-myristate 13-acetate (PMA, 250 nM), an activator of protein kinase C (PKC), was used to induce vascular endothelial activation. Adenoviral TSPO overexpression (10-100 MOI) inhibited PMA-induced vascular cell adhesion molecule-1 (VCAM-1) and intracellular cell adhesion molecule-1 (ICAM-1) expression in a dose dependent manner. PMA-induced VCAM-1 expressions were inhibited by Mito-TEMPO ($0.1-0.5{\mu}m$), a specific mitochondrial antioxidants, and cyclosporin A ($1-5{\mu}m$), a mitochondrial permeability transition pore inhibitor, implying on an important role of mitochondrial reactive oxygen species (ROS) on the endothelial activation. Moreover, adenoviral TSPO overexpression inhibited mitochondrial ROS production and manganese superoxide dismutase expression. On contrasts, gene silencing of TSPO with siRNA increased PMA-induced VCAM-1 expression and mitochondrial ROS production. Midazolam ($1-50{\mu}m$), TSPO ligands, inhibited PMA-induced VCAM-1 and mitochondrial ROS production in endothelial cells. These results suggest that mitochondrial TSPO can inhibit PMA-induced endothelial inflammation via suppression of VCAM-1 and mitochondrial ROS production in endothelial cells.

• International journal of advanced smart convergence
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• v.1 no.2
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• pp.1-11
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• 2012

IP-based Wireless Sensor Networks (IP-WSNs) are gaining importance for their broad range of applications in health care, home automation, environmental monitoring, industrial control, vehicle telematics, and agricultural monitoring. In all these applications, a fundamental issue is the mobility in the sensor network, particularly with regards to energy efficiency. Because of the energy inefficiency of network-based mobility management protocols, they can be supported via IP-WSNs. In this paper, we propose a network-based mobility-supported IP-WSN protocol called mSFP, or the mSFP: "Multicasting-supported Inter-Domain Mobility Management Scheme in Sensor-based Fast Proxy Mobile IPv6 Networks". Based on [8,20], we present its network architecture and evaluate its performance by considering the signaling and mobility cost. Our analysis shows that the proposed scheme reduces the signaling cost, total cost, and mobility cost. With respect to the number of IP-WSN nodes, the proposed scheme reduces the signaling cost by 7% and the total cost by 3%. With respect to the number of hops, the proposed scheme reduces the signaling cost by 6.9%, the total cost by 2.5%, and the mobility cost by 1.5%. With respect to the number of IP-WSN nodes, the proposed scheme reduces the mobility cost by 1.6%.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.10a
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• pp.93-96
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• 2001

For interconnecting PSTN and IP Networks, We made a comparative analysis of signaling interworking and also studied SS7 Signaling process as a method for signaling interworking for using the establishment of PATN/IP network and seting up a phone call. As a methode of signalning interworking for linking an intelligent net-service between PSTN/IP Networks, we made researches of the merits and demerits about the access of INAP/SS7 using PSTN SCP and the call-routing of intelligent net-service using PSTM SSP in an IP network. We made a study of the protocol stack in IETF SIGTRAN as the realization for this

• The Plant Pathology Journal
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• v.27 no.2
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• pp.170-182
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• 2011

Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection were selected and their T-DNA insertion mutants were obtained from SALK institute. To elucidate the role of each gene in defense response, bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 was inoculated onto individual T-DNA insertion mutants. Four mutants exhibited decreased resistance and five mutants displayed significantly enhanced resistance against Pst DC3000-infection as measured by change in symptom development as compared to wild-type plants. Among them, member of uridin diphosphate (UDP)-glycosyltransferase (UGT) was of particular interest, since a UGT mutant (At1g05680) showed enhanced resistance to Pst-infection in Arabidopsis. In systemic acquired resistance (SAR) assay, this mutant showed enhanced activation of SAR. Also, the enhanced SAR correlated with increased expression of defense-related gene, AtPR1. These results emphasize that the glycosylation of UGT74E2 is a part of the SA-mediated disease-resistance mechanism.

• Journal of dental hygiene science
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• v.18 no.2
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• pp.130-135
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• 2018

The aim of this study was to investigate the role of osteal macrophages (osteomac) and osteocytes in bone remodeling using a mathematical model. We constructed the bone system with pre-osteoblasts, osteoclasts, osteocytes, and osteomac. Each link of the parameters and ordinary differential equations followed the Graham's model in 2013 except for the parameters of osteomac signaling and osteocytes signaling to link preosteoblasts and osteoblasts. We simulated the changes in each cell and bone volume according to the changes in the parameters of osteomac signaling and osteocytes signaling. The results showed bone volume was unstable and decreased gradually when the effectiveness of osteocytes and osteomac dropped below a certain level. When the parameters of osteomac signaling and osteocytes signaling to link preosteoblasts and osteoblasts had a value less than 1, bone volume increased with the increase in the parameter of osteomac signaling to link preosteoblasts and osteoblasts. Moreover, although the parameter of osteocytes signaling to link preosteoblasts and osteoblasts, increased in case of a small parameter of osteomac signaling, bone volulme decreased. If the parameters of osteomac signaling to link preosteoblasts and osteoblasts were over a certain level, bone volume was positively maintained, despite the parameter of osteocyte signaling to link preosteoblasts and osteoblasts. We suggested the osteomac may affect bone remodeling and may play an important role in bone cell network.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.11
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• pp.1988-1991
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• 2007

This study investigates the performances of the signaling techniques including differential signals for the power-distribution network(PDN)s with and without the slit, using a rigorous evaluation method 'Modal Analysis', validated by being compared to the FDTD simulation up to 5 GHz.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.2
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• pp.275-279
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• 2005

In this paper, we analyses of a traffic for authentication signaling in third generation mobile sensor network. In universal mobile telecommunication system, authentication functions are utilized to identify and authentication a mobile station and validate the service request network services. The authenticating parties are the authentication the serving general packet radio service support node access the authentication center to obtain the authentication with the mobile station. In this paper, we propose that the automatic cost-effective solution size of the authentication vector array.