Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant effect of Raphani Semen (Raphanus sativus L.)

나복자의 항산화 효과

  • Seon Been, Bak (School of Korean Medicine, Dongguk University) ;
  • Seung-Ho, Kang (Daegu Haany University) ;
  • Kwang-Il, Park (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Won-Yung, Lee (School of Korean Medicine, Dongguk University)
  • 박선빈 (동국대학교 한의과대학 방제학교실) ;
  • 강승호 (대구한의대학교 ) ;
  • 박광일 (경상대학교 수의과대학 수의생리학) ;
  • 이원융 (동국대학교 한의과대학 방제학교실)
  • Received : 2023.02.14
  • Accepted : 2023.02.17
  • Published : 2023.02.28
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Abstract

Objectives : Raphani Semen (Raphanus sativus L.) is known for the various beneficial effects in Korean medicine. This study aimed to investigate the effect of Raphani Semen extract (RSE) against arachidonic acid (AA)+iron-induced oxidative stress in cells. Methods : Ingredients, their target information, oxidative stress liver injury-related proteins was obtained from various network pharmacology databases and software. A hypergeometric test and enrichment analysis were conducted to evaluate associations between protein targets of RSE. The cell viability was assessed by MTT assay, and immunoblot analysis was used to confirm the molecular mechanisms. Results : A compound-target network of RSE was constructed, which consisted of 336 edges between 18 ingredients and 123 protein targets. PI3K-Akt signaling pathway, ErbB signaling pathway, HIF-1 signaling pathway, PPAR signaling pathway, and AMPK signaling pathway was significantly associated with protein targets of RSE. RSE protected HepG2 cells against AA+iron-induced oxidative stress as mediated with AMPK signaling. Conclusion : RSE was found to protect the cells against oxidative stress via the AMPK signaling pathway.

Keywords

Acknowledgement

Bak SB would like to thank to the Ph.D.'s program of Dongguk University for completing the Ph.D. thesis through this work.

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