• 약학회지
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• 제46권6호
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• pp.398-404
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• 2002

A phytochemical study on the root of Cibotium barometz J. Smith led to the isolation of onitin (I), daucosterol (II) and a new compound (III). Compound III was characterized as 2-Ο-(9Z,12Z-octadecadienoyl)-3-Ο-[$\alpha$-D-galactopyranosyl-(1"-6")-Ο-$\beta$-D-galactopyranosyl] glycerol, named shinbarometin by $^1$H-, $^{13}$ C-NMR and LC/MS data. Compound III exerted an induced neuronal regeneration on nogo-A induced neuroblastoma cells.

• 한국작물학회지
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• 제47권5호
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• pp.352-355
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• 2002

Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/$\ell$ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/$\ell$ of NAA and 1 mg/$\ell$ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/$\ell$ of 2,4-D, and 3 mg/$\ell$ of 2,4-D and 3 mg/$\ell$ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/$\ell$ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.

• 대한치과의사협회지
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• 제32권1호통권296호
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• pp.47-54
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• 1994

• Plant Biotechnology Reports
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• 제3권3호
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• pp.175-182
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• 2009

Simple, reproducible, high frequency, improved plant regeneration protocol in Eastern Cottonwood (Populus deltoides) clones, WIMCO199 and L34, has been reported. Initially, aseptic cultures established from axillary buds of nodal segments from mature plus trees on MS liquid medium supplemented with $0.25mg\;1^{-1}$ KIN and $0.25mg\;1^{-1}$ IAA. Nodal and internodal segments were found to be extra-prolific over shoot apices during course of aseptic culture establishment, while $0.25mg\;1^{-1}$ KIN concentration played a stimulatory role in high frequency plant regeneration. Diverse explants, such as various leaf segments, internodes, and roots from in vitro raised cultures, were employed. Direct plant regeneration was at high frequency of 92% in internodes, 88% in leaf segments, and 43% in root segments. This led to the formation of multiple shoot clusters on established culture media with rapid proliferation rates. Many-fold enhanced shoot elongation and growth of the clusters could be achieved on liquid MS medium supplemented with borosilicate glass beads, which offer physical support for proliferating shoots leading to faster growth in comparison to semi-solid agar or direct liquid medium. SEM examination of initial cultures confirmed direct plant regeneration events without intervening calli. In vitro regenerated plants induced roots on half-strength MS medium with $0.15mg\;1^{-1}$ IAA. Rooted 5- to 6-week-old in vitro regenerated plants were transferred into a transgenic greenhouse in pots containing 1:1 mixture of vermicompost and soil at $27{\pm}2^{\circ}C$ for hardening and acclimatization. 14- to 15-week-old well-established hardened plants were transplanted to the field and grown to maturity. The mature in vitro raised poplar trees exhibited a high survival rate of 85%; 4-year-old healthy trees attained an average height of 8 m and an average trunk diameter of 25 cm and have performed well under field conditions. The regeneration protocol presented here will be very useful for undertaking genetic manipulation, providing a value addition to Eastern Cottonwood propagation in future.

• Plant Resources
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• 제4권3호
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• pp.111-120
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• 2001

The efficiency of in vitro regeneration of four clones of Populus euramericana, Canada blanc, Eugenii, I-45/51, and Wisconsin #5, was examined. Cytokinins and the combinations with auxins affected the rate of regeneration from the explants of root segments, stem internodes, and leaf discs. Overall, BA and the combination with auxins were effective in root segments and leaf discs of the Canada blanc clone, whereas zeatin and the combination with auxins were important in stem internodes of the Wisconsin #5 clone. The highest number of shoots averaging 17.6 $\pm$ 0.47 from root segments in the Canada blanc clone,18.2 $\pm$ 3.0 from stem internodes in the Wisconsin #5 clone, and 17.8 $\pm$ 1.92 from leaf discs in the Canada blanc clone were obtained with 2.0 mg/1 BA, 2.0 mg/l zeatin combined with 0.2 mg/l IAA, and 0.5 mg/l BA combined with 0.05 mg/l 2,4-D, respectively. In particular, the addition of 2,4-D into cytokinin medium promoted shoot proliferation.

• Plant Resources
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• 제4권2호
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• pp.65-74
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• 2001

Coleus forskohlii Briq, of the family Lamiaceae yields a valuable secondary metabolity known as forskolin which is a labdane diterpenoid.. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root rot disease. The fungal culture filterate (ECE) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% ECF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forshohlii. This data could prove to be useful for the future for selecting a resistant C.forskohlii plant against the root disease caused by L. theobromae.

• 한국약용작물학회지
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• 제14권4호
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• pp.225-228
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• 2006

식물생장조절제의 종류와 농도를 모색하여 할미꽃의 발근 최적 조건을 조사하여 정상적인 재분화체를 획득하고자 하였다. 지금까지의 선행연구를 토대로 최적조건에서 shoot를 유도시킨 후 발근 유도 생장조절제에 바로 옮기면 shoot가 너무 작아 재분화체가 성장하지 못하고 고사되었다. 1/2 MS에서 키운 shoot를 3.0 mg/L NAA를 첨가한 배지에서 3일간 생장시킨 후, 1/3 MS로 옮겨 치상 한 경우, 약 10일 전후에 뿌리의 유도가 관찰되었다. 이렇게 뿌리가 유도된 재분화체는 염의 농도와 설탕의 농도를 동시에 순차적으로 낮추어 순화처리되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.96-113
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• 2000

Coleus forskohlii Briq. of the family Lamiaceae yields a valuable secondary metabolite known as forskolin which is a labdane diterpenoid. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root of disease. The fungal culture filterate (FCF) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% FCF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forskohlii. This data could prove to be useful for the future for selecting a resistant C,forskohlii plant against the root disease caused by L.theobromae.

• 한국초지조사료학회지
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• 제23권2호
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• pp.95-100
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• 2003

알팔파 종자를 $3\;mg/{\ell}$의 2,4-D를 첨가한 SH 배지에서 배양하여 캘러스를 유도하였으며, 캘러스를 SH 배지에 $5\;mg/{\ell}$의 NAA와 $2\;mg/{\ell}$의 kinetin을 첨가한 배지에서 28~30일간 배양하고, $11\;mg/{\ell}$의 2,4-D와 $1\;mg/{\ell}$의 kinetin을 첨가한 배지에서 3~5일간 배양 후, $1.6\;g/{\ell}$의 (${NH_4}_2{SO_4}$와 $5.75\;g/{\ell}$의 proline을 첨가한 배지에서 21~25일간 배양하여 완전히 재분화된 식물체와 많은 수의 shoot를 얻었다. 알팔파의 재분화 과정에서 뿌리가 유도되는 효율을 높이기 위해, SH salt의 양과 호르몬 양을 달리한 8종의 배지에서 각각 뿌리가 유도되는 정도를 조사해 본 결과, SH 기본배지를 사용한 것에 비해 IBA를 첨가했을 때 뿌리 유도율이 높았으며, 배지 1 리터당 1.5 mg의 IBA를 첨가했을 때 부리 유도율이 56.0%로서 가장 높았다. 또한 SH salt의 양을 1/2로 줄이고 IBA 첨가량을 다르게 하여 뿌리 유도율을 조사했을 때, SH 기본배지를 사용한 것에 비해 SH salt의 양을 1/2로 줄이고 뿌리 유도율이 높아졌으며, IBA를 첨가했을 때 푸리 유도율이 높았다. 이상의 결과에서 알팔파의 뿌리를 유도하기 위한 배지는, IBA 첨가량을 $1.5\;mg/{\ell}$로 하고 SH salt의 양을 1/2로 줄여서 사용하는 것이 좋을 것으로 판단된다.

• 대한간호학회지
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• 제36권3호
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• pp.523-531
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• 2006

Purpose: The purpose of this study was to examine the effects of the ulmus root-bark dressing on tissue regeneration in experimentally-induced pressure ulcers in rats. Method: A randomized pretest/post-test control group time-series study design was used. Thirty-three male Sprague-Dawley rats were used in this study. The rats were anesthetized with 100mg/kg of ketamine. Pressure ulcers were induced at 140mmHg for three hours using a personally-designed pressing apparatus. For four weeks, the ulmus root-bark dressing was applied every other day in the experimental group (n=18) and a wet gauze dressing in the control group (n=15). For data analysis, the statistical program SPSS WIN 12 was used. The wounds were examined by light microscopy andelectron microscopy. Result: There were significant statistical differences in the size of the pressure ulcers as time went by(p=0.006). It should be noted that there were no significant statistical differences in the number of capillaries. Using light microscopy the inflammatory infiltration and neovascularization in the dermis in the experimental group emerged densely in the early stages, but recovered rapidly at the latter stages. In addition, the reepithelization of the epidermis occurred earlier than in the control group. By electron microscopy, the cell organelles of the capillary endothelial cells and the basal lamina of capillaries in the experimental group showed a more rapid maturation during the latter stages, compared with the control group. Conclusion: According to this study, it can be concluded that the ulmus root-bark dressing is effective regarding the healing of pressure ulcers.