KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Callus Induction and Plant Regeneration from Mature Embryos in Oat

  • Published : 2002.12.01
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Abstract

Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/$\ell$ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/$\ell$ of NAA and 1 mg/$\ell$ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/$\ell$ of 2,4-D, and 3 mg/$\ell$ of 2,4-D and 3 mg/$\ell$ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/$\ell$ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.

Keywords

References

  1. Barrueto Cid, L. P., A. C. M. G. Machado, S. B. R. C. Caralheira, and A. C. M. Brasileiro. 1999. Plant regeneration from seedling explants of Eucalyptus grandis $\times$ E. urophylla. PIant cell, Tissue and Organ Culture 56 : 17-23 https://doi.org/10.1023/A:1006283816625
  2. Birsin, M. A., S. $\"{O}$nde, and M. $\"{O}$zgen. 2001. Callus induction and plant regeneration from mature embryos of oat (Avena sativd). Turk Biol. 25:427-434
  3. Bregitzer, P., W. R. Bushnell, H. W. Rines, and D. A. Somers. 1991. Callus formation and plant regeneration from somatic embryos of oat (Avena sativd). PIant Cell Rep. 10 : 243-246
  4. Bregitzer, P., D. A. Somers, and H. W. Rines. 1989. Development and characterization of friable, embryogenic oat callus. Crop Sci. 29: 798-803 https://doi.org/10.2135/cropsci1989.0011183X002900030052x
  5. Carter, O., Y. Yamada, and E. Takahashi. 1967. Tissue culture of oats. Nature 214 : 1029-1030 https://doi.org/10.1038/2141029a0
  6. Chen, H., G. Xu, D. C. Loschke, L. Tomaska, and B. G. Rolfe. 1995a. Efficient callus formation and plant regeneration from leaves of oats (Avena sativd). Plant Cell Rep. 14 : 393-397
  7. Chen, Z., Q. Zhuge, and C. Sundqvist. 1995b. Oat leaf base: tissue with an efficient regeneration capacity. PIant Cell Rep. 14 : 354-358
  8. Chu, C. C., C. C. Wang, C. S. Sun, C. Hsu, K. C. Yin, C. Y. Chu, and F. Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 18 : 659-688
  9. Cummings, D. P., C. E. Green, and D. D. Stuthman. 1976. Callus induction and plant regeneration in oats. Crop Sci. 16 : 465-470 https://doi.org/10.2135/cropsci1976.0011183X001600040006x
  10. Gless, C., H. L$\"o$rz and A. J$\"a$hne-G$\"a$rtner. 1998. Establishment of ahighly effcient regeneration system from leaf base segments of oat (Avena sativa L.). PIant Cell Rep. 17 : 441-445 https://doi.org/10.1007/s002990050422
  11. Heyser, J. W. and M. W. Nabors. 1982. Long term plant regeneration, somatic embryogenesis, and green spot formation in secondary oat (Avena sativd) callus. Z Pflanzenphysiol. 107 : 153-160 https://doi.org/10.1016/S0044-328X(82)80101-3
  12. Kiviharju, E., M. Puolimatka, M. Saastamoinen, and E. Pehu. 2000. Extention of anther culture to several genotypes of cultivated oats. Plant CelI Rep. 19: 674-679 https://doi.org/10.1007/s002999900165
  13. Kiviharju, E., M. Puolimatka, M. Saastamoinen, S. Hovinen, and E. Pehu. 1998. The effect of genotype on anther culture response of cultivated and wild oats. Agri. Food Sci. Finl. 7 : 409-422
  14. Kiviharju, E. and A. Tauriainen. 1999. 2,4-dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses: plant regeneration from Avena saliva L. PIant CelI Rep. 18 : 582-588 https://doi.org/10.1007/s002990050626
  15. L$\"o$rz, H., C. T. Harms, and I. Potrykus. 1976. Regeneration of plants from callus in Avena sativa. L. Z Pflanzenzuecht 77 : 257-259
  16. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  17. $\"{O}$zgen, M., M. T$\"u$ret, S. Altinok, and C. Sancak. 1998. Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant CeII Rep. 18:331-335 https://doi.org/10.1007/s002990050581
  18. Rines, H. W. and T. J. McCoy. 1981. Tissue culture initiation and plant regeneration in hexaploid species of oats. Crop Sci. 21 : 837-842 https://doi.org/10.2135/cropsci1981.0011183X002100060010x
  19. Rines, H. W. 1983. Oat anther culture: genotype effects or callus initiation and production of a haploid plant. Crop Sci. 23 : 268-272 https://doi.org/10.2135/cropsci1983.0011183X002300020022x
  20. Rines, H. W. and H. H. Luke. 1985. Selection and regeneration of toxin-in-sensitive plants from tissue cultures of oats (Avena sativd) susceptible to Helminthosporium victorias. Theor. Appl. Genet. 71 : 16-21 https://doi.org/10.1007/BF00278247
  21. Sears, R. G. and E. L. Deckard. 1982. Tissue culture variability in wheat : callus induction and plant regeneration. Crop Sci. 22 : 546-550 https://doi.org/10.2135/cropsci1982.0011183X002200030027x
  22. Seong, K. S. and J. K. Sohn. 1990. Some factors affecting callus formation and plant regeneration in the seed culture of rice (Oiyza sativa L.). Korean J. PIant Tissue Culture 17 : 23-32
  23. Shewkhawat, N. S., P. N. Gordon, and A. W. Galston. 1984. Highly regenerative tissue cultures from axillary tiller cultures of sexually mature oat plants, In Vitro CeII. Dev. Biol. 20 : 707-711
  24. Torbert, K. A., H. W. Rines, H. F. Kaeppler, G. K. Menon, and D. A. Somers. 1998b. Genetically engineering elite oat cultivars. Crop Sci. 38 : 1685-1687 https://doi.org/10.2135/cropsci1998.0011183X003800060044x
  25. Torbert, K. A., H. W. Rines, and D. A. Somers. 1998a. Transformation of oat using mature embryo derived tissue cultures. Crop Sci. 38 : 226-231 https://doi.org/10.2135/cropsci1998.0011183X003800010038x
  26. Zhang, S., H. Zhong, and M. B. Sticklen. 1996. Production of multiple shoots from shoot apical meristems of oat (Avena sativa L.).J. Plant Physiol. 148 : 667-671 https://doi.org/10.1016/S0176-1617(96)80365-8