• Maxillofacial Plastic and Reconstructive Surgery
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• 제22권2호
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• pp.164-173
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• 2000

The p16 protein is a cyclin dependent kinase inhibitor that inhibits cell cycle progression from $G_1$ phase to S phase in cell cycle. Many p16 gene mutations have been noted in many cancer-cell lines and in some primary cancers, and alterations of p16 gene function by DNA methylation have been noticed in various kinds of cancer tissues and cell-lines. There have been a large body of literature has accumulated indicating that abnormal patterns of DNA methylation (both hypomethylation and hypermethylation) occur in a wide variety of human neoplasma and that these aberrations of DNA methylation may play an important epigenetic role in the development and progression of neoplasia. DNA methylation is a part of the inheritable epigenetic system that influences expression or silencing of genes necessary for normal differentiation and proliferation. Gene activity may be silenced by methylation of up steream regulatory regions. Reactivation is associated with demethylation. Although evidence or a high incidence of p16 alterations in a variety of cell lines and primary tumors has been reported, that has been contested by other investigators. The precise mechanisms by which abnormal methylation might contribute to carcinogenesis are still not fully elucidated, but conceivably could involve the modulation of oncogene and other important regulatory gene expression, in addition to creating areas of genetic instability, thus predisposing to mutational events causing neoplasia. There have been many variable results of studies of head and neck squamous cell carcinoma(HNSCC). This investigation was studied on 13 primary HNSCC for p16 gene status by protein expression in immunohistochemistry, and DNA genetic/epigenetic analyzed to determine the incidence, the mechanisms, and the potential biological significance of its Inactivation. As methylation detection method of p16 gene, the methylation specific PCR(MSP) is sensitive and specific for methylation of any block of CpG sites in a CpG islands using bisulfite-modified DNA. The genomic DNA is modified by treatment with sodium bisulfate, which converts all unmethylated cytosines to uracil(thymidine). The primers designed for MSP were chosen for regions containing frequent cytosines (to distinguish unmodified from modified DNA), and CpG pairs near the 5' end of the primers (to provide maximal discrimination in the PCR between methylated and unmethylated DNA). The two strands of DNA are no longer complementary after bisulfite treatment, primers can be designed for either modified strand. In this study, 13 paraffin embedded block tissues were used, so the fragment of DNA to be amplified was intentionally small, to allow the assessment of methylation pattern in a limited region and to facilitate the application of this technique to samlples. In this 13 primary HNSCC tissues, there was no methylation of p16 promoter gene (detected by MSP and automatic sequencing). The p16 protein-specific immunohistochemical staining was performed on 13 paraffin embedded primary HNSCC tissue samples. Twelve cases among the 13 showed altered expression of p16 proteins (negative expression). In this study, The author suggested that low expression of p16 protein may play an important role in human HNSCC, and this study suggested that many kinds of genetic mechanisms including DNA methylation may play the role in carcinogenesis.

• 한국조경학회지
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• 제39권5호
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• pp.111-118
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• 2011

건강과 삶의 질 향상에 대한 관심이 커짐에 따라 도시녹지가 가져다 주는 건강편익이 크게 주목되고 있지만, 이에 관한 객관적이고 과학적인 데이터는 매우 부족한 실정이다. 따라서 본 연구에서는 도시녹지가 가져다 주는 건강편익에 대한 과학적 자료를 얻기 위해 생리적, 심리적 지표를 활용하여 실험을 실시하였다. 과거병력이 없는 20대 남자 대학생 20명이 실험에 참가하였고, 자극은 녹지와 도시에서 실제의 경관을 15분간 감상하는 것으로 하였다. 이번 연구는 충남대학교 의학전문대학원 생명윤리심사위원회의 승인을 받은 후 실시되었다. 녹지와 도시에 대한 생리반응을 분석한 결과, 도시에 비해 녹지에서 심박동수가 현저히 감소하였고, 안정상태에서 증가하는 부교감신경활동이 유의하게 향상되었으며, 스트레스호르몬의 일종인 코티솔 농도가 낮아지는 경향이 보였다. 심리반응에 있어서는 부정적인 감정과 정신상태가 녹지에서 보다 유의하게 낮아진 반면, 활력은 유의하게 증가하였다. 이번 연구의 결과는, 녹지를 접하는 것이 심리적 변화뿐만 아니라 인체의 자율신경계와 내분비계 활동에 긍정적 변화를 가져다 준다는 것을 보여주는 것으로써, 녹지가 도시민의 건강증진과 관련하여 매우 직접적인 환경요인이 될 수 있음을 과학적으로 뒷받침한다고 할 수 있다.

• 헤리티지:역사와 과학
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• 제46권3호
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• pp.78-93
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• 2013

본 연구는 자연유산의 미래의 수요자인 대학생들을 대상으로 천연기념물, 명승으로 대별되는 자연유산과 국 도립공원, 생태 경관보전지역과 같은 유사 개념을 얼마만큼 올바르게 인식하고 있는지를 알아보고, 보존 중요성 등 태도차원에서 각 개념 간의 경쟁구조를 분석하고자 하였다. 연구 결과, 보존의 중요도는 '천연기념물>생태 경관보전지역>국 도립공원>명승'의 순으로 나타나 천연기념물보다는 명승을 상대적으로 덜 보존적으로 인식하고 있다는 것을 알 수 있었다. 또한 순천만, 설악산, 창녕 우포늪, 홍도, 독도, 문경새재, 한라산, 성산일출봉, 영월 고씨굴, 태안 신두리 해안사구 포함 관광명소 10개소를 대상으로 자연유산 유형을 올바르게 인식하고 있는지를 대응분석한 결과, 전반적으로 응답자들은 자연유산에 대한 개념을 바르게 인식하고 있지 않다는 것을 파악할 수 있었으며, 이러한 문제점을 해결할 수 있도록 인식제고 프로그램 마련이 필요하다. 다차원 척도법을 활용하여 자연유산 유사개념 경쟁구조(유사성)를 파악한 결과, 포지셔닝 맵에서 천연기념물과 명승이 비교적 멀리 떨어져 나타난 것을 볼 때 각기 차별화되는 방문자 관리가 요구된다. 포지셔닝 맵 상에 나타난 자연유산 유사개념 경쟁구조와 각 개념에 대한 태도를 구성하는 속성결합의 결과를 살펴보면, 자연유산을 비롯한 네 개념 모두가 뚜렷한 차이를 보이지 않은 것으로 보아 향후 차별화된 홍보전략이 필요하다. 응답자들도 자연유산을 활용보다는 보존의 대상으로 생각하는 경향이 있는 것으로 파악되며, 다양한 관리주체 때문에 천차만별인 관리 수준을 체계화할 필요가 있다고 여겨진다.

• 한국환경복원기술학회지
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• 제21권6호
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• pp.55-69
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• 2018

As disaster risk and climate change volatility increase, there are more efforts to adapt to disasters such as forest fires, floods, and landslides. Most of the research, however, is about influence of human activities on disaster and there is few research on disaster adaptation for species. Previous studies focusing on biodiversity in selecting conservation areas have not addressed threats of disaster in the habitats for species. The natural disasters sometimes play role of drivers of ecological successions in the long run, but they might cause serious problems for the conservation of vulnerable species which are endangered. The purpose of this study is to determine whether soil loss (SL) is effective in selecting habitat management areas for amphibians and reptiles. RUSLE model was used to calculate soil loss (SL) and the distribution of each species (SD) was computed with MaxEnt model to find out the biodiversity index. In order to select the habitat management area, we estimated the different results depending if value of soil loss was applied or not by using MARXAN, a conservation priority selection tool. With using MARXAN, conservation goals can be achieved according to the scenario objectives, and the study has been made to meet the minimum habitat area. Finally, the results are expressed in two; 1) the result of soil loss and biodiversity with MATRIX method and 2) the result of regional difference calculated with MARXAN conservation prioritization considering soil loss. The first result indicates that the area with high soil loss and low species diversity have lower conservation values and thus can be managed as natural disturbances. In the area where soil loss is high and species diversity is also high, it becomes where a disaster mitigation action should be taken for the species. According to the conservation priorities of the second result, higher effectiveness of conservation was obtained with fewer area when it considered SL in addition to SD, compared to when considered only biodiversity. When the SL was not taken into consideration, forest area with high distribution of species were important, but when SL considered, the agricultural area or downstream of the river were represented to be a major part of habitats. If more species data or disaster parameters other than soil loss are added as variables later, it could contribute as a reference material for decision-making to achieve various purposes.

• 지역연구
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• 제37권2호
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• pp.17-33
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• 2021

이 연구는 코로나바이러스감염증-19(이하 COVID-19) 확산 및 이에 따른 사회적 거리두기의 시행이 공원 이용자 수에 미치는 효과를 실증적으로 분석하였다. 이를 위해 서울시 송파구에 소재한 대규모 종합도시공원인 올림픽공원과 동일 자치구 내 근린공원 4 곳의 이용자 수와 COVID-19 발생에 관한 시계열 자료를 분석하고, 이를 서울의 대표적 실내 복합여가공간인 잠실 롯데월드 일대 이용자 수 변화 효과와 비교하였다. 분석결과는 다음과 같다. 먼저, 주거지역 내 위치한 소규모 근린공원은 COVID-19 확산과 이에 따른 사회적 거리두기 시행으로 발생 이전에 비해 평균적으로 3~6% 정도 이용자 수가 증가하였다. 특히 사회적 거리두기 강도 상향에 따라 공원 이용변화가 민감한 것으로 나타났다. 반면 잠실 롯데월드 일대 이용자 수가 평균적으로 38%가량 감소하였고, 올림픽공원의 경우에는 COVID-19 확산에 따라 평균적으로 1.9% 정도 이용자 수가 감소하였다. 올림픽공원의 원거리 이용자를 나타내는 차량 이용자 수가 평균적으로 23%가량 감소한 것을 고려하면, 주변지역 이용자 수는 거의 변화가 없는 것으로 추정된다. 이는 COVID-19와 같은 전염병 재난 시 도시공원, 특히 주거지역 내 근린공원은 도시인들에게 주요한 피난처이자 여가공간으로서 역할을 수행하므로 이의 적절한 공급과 유지관리가 필요하다는 것을 시사한다.

• 융합보안논문지
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• 제20권4호
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• pp.227-236
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• 2020

냉전시기 군사적 활동을 위주로 이루어졌던 전통적 PKO 활동은 탈냉전 시기가 되면서 다차원적 PKO 활동으로 변해왔다. 한국경찰은 1994년 소말리아 치안재건을 위해 2명의 경찰관을 파견하면서 PKO의 역사가 시작되었으나 지금까지 군 병력의 활발한 PKO 활동에 비해 경찰의 역할은 제한적으로 이루어졌다. 국제분쟁의 양상이 지속적으로 진보하고 있는 현시점에서 UN은 193개 회원국에게 다차원적 평화유지 과업을 해결할 수 있는 PKO 활동을 요구하고 있으며, 특히 경찰의 '치안유지'라는 본질적 역할은 분쟁국의 재건과정에서 엄정한 법집행을 절대적으로 요구하고 있다. 이에 미국, 중국, 독일 등 회원국에서는 경찰 인력을 파견하여 그 역할을 직·간접적으로 수행하고 있다. 그러나 한국경찰의 PKO에 대한 평가는 그 성과를 측정하기에 미미한 수준이다. 따라서 본 연구에서는 설정한 연구목적을 달성하기 위해 먼저 국제분쟁의 양상과 분쟁지역의 치안환경을 분석하였다. 그리고 다차원적 PKO 임무 속에서 UN경찰의 역할과 미국, 중국, 독일 경찰과 한국 경찰의 사례를 분석하였으며, 이를 토대로 제한적이었던 한국경찰 PKO 요원의 역할 확대 방안을 제시할 수 있었다.

• 풍력에너지저널
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• 제15권1호
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• pp.5-10
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• 2024

Offshore wind power generation has significant advantages, including enhanced energy security and job creation. However, despite these benefits, South Korea has not fully utilized its potential in this sector. In contrast, offshore wind power industry development in Europe has been driven by government leadership. Drawing from this experience, South Korea also needs to relax regulations, strengthen necessary infrastructure, and enhance financial support systems to activate the offshore wind power industry. For this, sustained government leadership is absolutely essential. Without addressing the capacity issues in the power grid, we cannot expect offshore wind power generation to succeed. To address grid issues, we propose the enactment of a special law called the "Special Act on Grid Expansion." Considering KEPCO's financial situation, private investment should be encouraged for grid construction. The role of developers is crucial for the successful development and operation of offshore wind power. They manage risks throughout various stages, from site acquisition to construction and operation, which have a significant impact on the success or failure of projects. Since domestic developers currently lack experience in offshore wind power, a cooperative strategy that leverages the experience and technology of advanced countries is necessary. Energy issues should be recognized as important tasks beyond mere political ideologies, as they are crucial for the survival of the nation and its development. It is essential to form a public consensus and implement ways for residents to coexist with offshore wind power, along with the conservation of marine ecosystems and effective communication with stakeholders. Expansion of the offshore wind power industry requires support in various areas, including financial and tax incentives, technology research investment, and workforce development. In particular, achieving carbon neutrality by 2050 necessitates the activation of offshore wind power alongside efforts by major corporations to transition to renewable energy. South Korea, surrounded by the sea, holds significant offshore wind power potential, and it is our responsibility to harness it as a sustainable energy source for future generations. To activate the offshore wind power market, we need to provide financial and tax support, develop infrastructure and research, and foster a skilled workforce. As major corporations transition to renewable energy to achieve carbon neutrality by 2050, offshore wind power must play a significant role. It is our responsibility to fully utilize South Korea's potential and make offshore wind power a new driver of growth.

• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.89-98
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• 2016

Endophytes play an important role in the growth and development of the host. However, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. We studied the bacteria associated with fruiting bodies of Tricholoma matsutake picked from seven main T. matsutake-producing areas in Sichuan, China, by barcoded pyrosequencing. About 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and archaea, and 361-797 operational taxonomic units were observed at a 97% similarity level. The bacterial community was always both more abundant and more diverse than the archaeal community. UniFrac analysis showed there were some difference of bacterial communities among the samples sites. Three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, were dominant in all samples. Correlation analysis showed there was a significant correlation between some soil properties and bacterial community associated with T. matsutake. This study demonstrated that the bacteria associated with T. matsutake fruiting bodies were diversified. Among these bacteria, we may find some strains that can promote the growth of T. matsutake.

• 한국응급구조학회지
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• 제13권1호
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• pp.61-72
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• 2009

Purpose : This study was conducted to understand the unforeseen phone calls placed through the 1339 emergency medical communication system by residents of the Gwangju/Jeonnam area during the Lunar New Year and Chuseok holidays. Methods : A descriptive research method was implemented using SPSS to analyze 9,047 reported and received consultation phone calls made during the 2008 Lunar New Year holiday (February 6-10) and the 2008 Chuseok holiday (September 13-15) for frequency and percentages. Results : Among the general characteristics of subjects during the Lunar New Year and Chuseok holidays, an examination of the distribution for gender showed more men than women and the distribution for age showed most subjects were thirties, followed by forties, and finally under ten years of age. According to the status of situational calls during the Lunar New Year holiday most were used for in the following order : providing resource information, misdialing and prank calls, and additional consultations. According to the status of situational calls during the Chuseok holiday most were used for in the following order : providing resource information, additional consultations, misdialing and prank calls. According to region most came from Gwangju Metropolitan City Bukgu, followed by Yeosu, Suncheon, and Mokpo in Jeonnam. According to callers, the majority were non-medical persons. According to time most were made between 9:01 and 12:00, while the fewest were made during 3:01 and 6:00. According to treatment department most were made in the following order : internal medicine, pharmacy, and pediatrics. Most medical resource information provided regarded areas outside the callers location. Conclusion : Emergency medical technician will play a central role in the emergency medical information system in the near future.

• 한국동물위생학회지
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• 제35권4호
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• pp.327-332
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• 2012

This study aimed to see if as companion animals living with human beings, dogs could provide emotional stability or play a therapeutic role for human beings. Of 12 middle school boys as victims of school violence, 6 were assigned to the experimental group that was provided with an animal-assisted therapy program over a total of 12 sections, one section per week, 60 minutes per section, between September and November 2011, followed by post-test 1 and, one month later, post-test 2. In the experimental composition, the remaining 6 boys were assigned to the comparison group that was allowed to read freely. Analysis was performed by using the scales of depression and self-esteem, and the results showed that the experimental group provided with the animal-assisted therapy program generated more significant therapeutic effects than the comparison group. The therapeutic effects were found to persist one month after the completion of the program. Therefore, it is believed that sensitive adolescents who suffer from depression or have lost self-esteem because of school violence can significantly benefit from an animal-assisted therapy program by reducing the recovery time or by getting lots of help with emotional stability. Further research on the basis of this study is expected to help adolescents with emotional therapy in other areas.