• Title/Summary/Keyword: replication control

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Ab ovo or de novo? Mechanisms of Centriole Duplication

  • Loncarek, Jadranka;Khodjakov, Alexey
    • Molecules and Cells
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    • 제27권2호
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    • pp.135-142
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    • 2009
  • The centrosome, an organelle comprising centrioles and associated pericentriolar material, is the major microtubule organizing center in animal cells. For the cell to form a bipolar mitotic spindle and ensure proper chromosome segregation at the end of each cell cycle, it is paramount that the cell contains two and only two centrosomes. Because the number of centrosomes in the cell is determined by the number of centrioles, cells have evolved elaborate mechanisms to control centriole biogenesis and to tightly coordinate this process with DNA replication. Here we review key proteins involved in centriole assembly, compare two major modes of centriole biogenesis, and discuss the mechanisms that ensure stringency of centriole number.

부분 중복 데이터베이스에서 사본 트리를 이용한 중복 제어 (The Replication Control using the Replica Tree in the Partially Replicated Databases)

  • 배미숙;황부현
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2003년도 추계학술발표논문집 (하)
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    • pp.1453-1456
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    • 2003
  • 데이터의 중복은 데이터의 가용성과 시스템의 성능을 향상시키기 위해 사용한다. 대규모 부분 중복 데이터베이스에서 효율적인 중복 관리를 위해서는 정확한 수행을 보장하기 위한 정확성 검증방법과 효율적인 갱신 전파 방법이 필요하다. 이 논문에서는 부분 중복 환경에서 각 주사본 사이트의 중복 데이터에 대한 트리 구조를 기반으로 한 갱신 전파 방법을 제시하며, 갱신 지연 전파로 인해 갱신 전파도중에 발생할 수 있는 전파 트랜잭션과 주 트랜잭션의 충돌로 인한 비직렬성 문제를 타임스탬프와 상태 데이터베이스를 이용하여 해결한다. 이것은 판독 가용성을 증가시키면서 비직렬성으로 인한 재수행을 회피하게 되어 트랜잭션의 완료율을 증가시킨다.

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제초제 처리방법이 메꽃방제 및 옥수수의 생산성에 미치는 영향 (Effects of Herbicide Application Method on Calysteria japonica Control and Corn Yield)

  • 정의수;김종근;강우성;서성;김경남
    • 한국초지조사료학회지
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    • 제18권3호
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    • pp.217-222
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    • 1998
  • This experiment was carried out to investigate effects of herbicide application method on Calysteria jcqoonica control and corn yield at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1996 to 1997. The experiment was arranged in a randomized block design with three replication. The herbicide application consisted of control, 2~3 leaf stage(Dicamba), 5~6 leaf stage(Dicamba) and soil treatment (Pendimethalin). The hybrid of corn used in this experiment was P 3352. The results obtained are summarized as follow; 1. The plant height was the lowest at control and the highest at Dicamba treatment in 5~6 leaf stage, but no significant difference was found among herbicide application method at ear height. Tasseling and silking date were delayed 2~3 day at control. The length and weight of ear were highest at Dicamba treatment in 2~3 leaf stage. 2. The herbicide injury of corn was very slightly after 10-20 day, but corn was recovered soon. The weed control value was 94.2~67.5% at herbicide application plot. 3. The fresh and dry matter yield of Dicamba treatment in 2-3 leaf stage was highest as 42,878 and 16,033kg/ha, respectively. The application of Dicamba increased the DM yield of corn by 13~30%. 4. Herbicide application time had little effect on the ADF, NDF and CP contents, but the forage quality of ear was higher than that of stover. The result of this experiment indicate that the application of Dicamba in 2~6 leaf stage of corn will remove almost all of Calysreria jqonica and increase DM yield of com.

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사료내 아마종실과 사료첨가물질의 조합첨가가 난황내 지방산 함량과 산란능력에 미치는 영향

  • 이준엽;이선연;오상집
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2002년도 가을 학술발표논문집
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    • pp.128-129
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    • 2002
  • 본 연구결과로 $\omega$-3 지방산을 포함한 지방산의 계란내 이행정도는 flaxseed의 첨가와 지방산대사에 영향을 미치는 미량 사료 첨가물질의 병용첨가로 변화되는 것으로 나타났으며, 특히 생균물질과 lecithin의 생리적 기능에 의한 계란내 $\omega$-3 지방산의 급증이 특이적인 것으로 나타났다. 그러나 미량 사료 첨가물질의 지방대사 조절기전에 관해서는 추가연구가 필요한 것으로 판단된다.

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폴리드나바이러스와 새로운 해충방제 전략 (Polydnavirus and Its Novel Application to Insect Pest Control)

  • 김용균
    • 한국응용곤충학회지
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    • 제45권3호
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    • pp.241-259
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    • 2006
  • 폴리드나바이러스는 고치벌 및 맵시벌류에 공생하는 DNA 바이러스로 기주 염색체에 프로바이러스 형태로 존재한다. 이 바이러스의 복제는 기주 용발육시기에 난소받침 상피세포에서 시작되어 유리 바이러스 형태의 입자 구조를 이루게 된다. 바이러스 입자는 기주가 피기생체에 산란할 때 알과 함께 혈강으로 옮겨진다. 이 바이러스 게놈의 염기서열을 바탕으로 여러 폴리드나바이러스 유전자군이 동정되었으며, 이들의 생리적 기능도 알려지고 있다. 본 종설은 기생 생리적 견지에서 폴리드나바이러스 게놈을 특성화하고, 이를 토대로 생리 교란 유전자들을 응용할 수 있는 새로운 해충 방제 전략을 소개한다.

스마트폰을 이용한 대장내시경 장정결 동영상 교육의 효과 (Effects of Video Assisted Education Using Smartphone on Bowel Preparation for Colonoscopy)

  • 최미희;송준아
    • 기본간호학회지
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    • 제24권1호
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    • pp.60-71
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    • 2017
  • Purpose: The purpose of this study was to develop video assisted education on bowel preparation for colonoscopy (VEBPC) and use a snartphone to evaluate effects of the VEBPC. Methods: Adult patients who were scheduled for colonoscopy were recruited from a university general hospital and randomly assigned to three groups. Group 1 (n=30) watched the video using a computer set in the endoscope consulting room. Group 2 (n=29) watched it using a smartphone, and group 3, the control group (n=29) received education with existing instructions at the reservation-reception desk. Participants were evaluated on knowledge on taking bowel preparation agents and diet, compliance on taking bowel preparation agents and diet, satisfaction with education, and actual level of bowel preparation. Results: Group 1 and 2 showed significantly (p<.001) higher scores for knowledge, compliance, and satisfaction compared to the control group. However, in post-hoc test analyses there were no significant differences in these variables between group 1 and 2. No significant difference was found in the actual level of bowel preparation among the three groups. Conclusion: Findings from this study show that VEBPC using smartphone is a better option than existing educational methods. However, replication studies are necessary to confirm these findings.

흰쥐의 일주기조절중추내 바이러스 감염에 대한 매개변수 분석 (Parametric Analysis on the Viral Infection in The Rat Circardian Control Center)

  • 김진상
    • The Journal of Korean Physical Therapy
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    • 제10권2호
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    • pp.113-125
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    • 1998
  • The neurotropic psudorabies virus(PRV) to replicate within neurons is very useful pathogen for neuronal tracing. I carried out this study to investigate the parametric analysis on the viral infection in the rat circadian control center with two genetically engineered strains out of PRV. The two strains are isogenic with the attenuated Bartha strain of PRV ; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu ; $4.75\times10^8pfu/ml$) and the other strain contained a PRV envelope glycoprotein gene(PRV-D ; $2.5\times10^8pfu/ml$) theat is absent in PRV-BaBlu. simultaneous or temporally separated sequential injection of$2{\mu}l$ of each strain into the vetreous body of eye produced a course of transsynaptic infection of retinohypothalamic circuitry. The results were as follows; 1. PRV-BaBlu and PRV-D infected the suprachiasmatic nucleus in hypothalamus and intergeniculate leaflet in lateral geniculate nucleus of thalamus. 2. The rate of PRV infection was dependent upon PRV strain. 3. Pre-infected neurons by PRV-D were interfered with the replication of PRV-BaBlu. 4. Dual injection of PRV-D and PRV-BaBlu showed more virulent than the parental strain.

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Previous Infection with Plasmodium berghei Confers Resistance to Toxoplasma gondii Infection in Mice

  • Lee, Dong-Hun;Chu, Ki-Back;Kang, Hae-Ji;Lee, Su-Hwa;Quan, Fu-Shi
    • Parasites, Hosts and Diseases
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    • 제57권2호
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    • pp.93-99
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    • 2019
  • Both Plasmodium spp. and Toxoplasma gondii are important apicomplexan parasites, which infect humans worldwide. Genetic analyses have revealed that 33% of amino acid sequences of inner membrane complex from the malaria parasite Plasmodium berghei is similar to that of Toxoplasma gondii. Inner membrane complex is known to be involved in cell invasion and replication. In this study, we investigated the resistance against T. gondii (ME49) infection induced by previously infected P. berghei (ANKA) in mice. Levels of T. gondii-specific IgG, IgG1, IgG2a, and IgG2b antibody responses, $CD4^+$ and $CD8^+$ T cell populations were found higher in the mice infected with P. berghei (ANKA) and challenged with T. gondii (ME49) compared to that in control mice infected with T. gondii alone (ME49). P. berghei (ANKA) + T. gondii (ME49) group showed significantly reduced the number and size of T. gondii (ME49) cysts in the brains of mice, resulting in lower body weight loss compared to ME49 control group. These results indicate that previous exposure to P. berghei (ANKA) induce resistance to subsequent T. gondii (ME49) infection.

Effect of dietary ractopamine supplementation on growth performance, meat quality and fecal score in finishing pigs

  • Hoque, Md-Raihanul;Im, Yu-Mi;Kim, In Ho
    • 농업과학연구
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    • 제47권4호
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    • pp.707-715
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    • 2020
  • An experiment was designed to assess ractopamine supplementation on growth, meat quality and carcass, and fecal scores of pigs. The 96 crossbred pigs ([Yorkshire× Landrace] × Duroc) had an average body weight of 72.8 ± 2.5 kg. Randomized allocation was followed in the allotment of the pigs according to their body weight into 1 of 2 dietary treatments: 1) CON, basal diet and 2) RAC (ractopamine hydrochloride), CON + 1% ractopamine with 12 replication pens per treatment including equal numbers of barrows (2) and gilts (2) per pen. At the end of the experiment, the lean meat percentage was higher (p < 0.05) for the RAC group compared to the CON group. Dietary ractopamine supplementation did not influence (p > 0.05) the growth performance and fecal score throughout the experimental period. In regard to sensory evaluation, higher (p < 0.05) scores for color and firmness were observed in the RAC group than in the CON group. Drip loss (p < 0.05) was higher in the ractopamine fed diet group compared to the control diet group at day 1. Additionally, longissimus muscle was improved (p < 0.05) in the ractopamine treated diet group than in the control diet group. In conclusion, in the early stage, ractopamine supplementation may not influence the growth performance, but it improves the leanness in the meat of finishing pigs.

건조 남은음식물 급여가 육성돈과 비육돈의 생산성에 미치는 효과 (Effects of Feeding Dried Leftover Food on Productivity of Growing and Finishing Pigs)

  • 조영무;김광현;고홍범;정일병;이지웅;배인휴;양철주
    • 유기물자원화
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    • 제12권2호
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    • pp.61-71
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    • 2004
  • 육성돈 시험은 평균체중이 약 22 kg 내외인 3원교잡종 ($Yorkshire{\times}Landrace{\times}Duroc$) 75두를 공시하여, 건조 남은음식물 무첨가구 (Control), 25%, 50% 첨가구와 사료내 단백질 함량을 10% 보강한 건조 남은음식물 25% 첨가구 및 단백질 함량을 20% 보강한 건조 남은음식물 50% 첨가구 등 총 5개 처리구로 하여 60일간 사양시험을 실시하였다. 육성돈의 일당증체량은 대조구가 모든 처리구중에서 가장 높았으나 건조 남은음식물 25% 첨가구와는 차이가 없었다 (P>0.05). 사료섭취량은 건조 남은음식물 첨가구가 대조구에 비해 다소 낮았으나 큰 차이는 없었고, 건조 남은음식물 첨가구중에서는 단백질을 20% 보강한 건조 남은음식물 50% 첨가구의 사료섭취량이 유의적으로 높았다 (P<0.05). 사료요구율은 처리간에 유의적인 차이는 없었으나, 건조 남은음식물 25% 첨가구가 다소 개선되는 경향이었다. 비육돈 시험은 평균체중이 약 70 kg 내외인 3원교잡종 ($Yorkshire{\times}Landrace{\times}Duroc$) 75두를 공시하여 49일간 사양시험을 실시하였으며, 처리내용은 육성돈의 시험과 같다. 비육돈의 일당증체량은 대조구에 비해 건조 남은음식물 첨가구가 유의적으로 낮았으나 단백질을 10% 보강한 건조 남은음식물 25% 첨가구는 대조구와 차이가 없었다 (P>0.05). 사료섭취량은 대조구에 비해 건조 남은음식물 첨가구가 유의적으로 감소하였으나 단백질을 보강한 건조 남은음식물 첨가구는 대조구와 차이가 없었다 (P>0.05). 사료요구율은 단백질 함량을 10% 보강한 건조 남은음식물25% 첨가구가 가장 낮았으나 대조구와는 차이가 없었고, 50%첨가구는 유의적으로 높았다 (P<0.05). 도체중은 건조 남은음식물의 첨가수준이 증가할수록 낮아지는 경향이었으며, 도체율, 등지방두께 및 도체등급은 처리간에 차이가 없었다. 비육돈의 1 kg 증체당 사료비는 단백질 함량을 10% 보강한 건조 남은음식물 25% 첨가구가 가장 적게 소요되었다.

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