• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.1
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• pp.9-15
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• 2006

As a part of the investigation for utilizing pearl oyster by-products, a rapid salt-fermented pearl oyster using commercial enzyme was prepared and also examined on the characteristics. The salt-fermented pearl oyster prepared by optimal condition, which was prepared by mixing of minced pearl oyster, 15% salt, and 1% $Protamex^\circledR$ and fermented for 4 weeks, was superior in hydrolysis degree (28.7%) and ACE inhibitory activity (92.6%) to salt-fermented pearl oyster prepared by other conditions, such as the use of whole tissue, different enzymes $(Alcalase^\circledR,\;Neutrase^\circledR\;and\;Flavourzyme^\circledR)$, different salt concentrations (20 and 25%), and different fermentation periods (2, 6 and 8 weeks). There were, however, some shortcomings with this product. It showed a dark green color and an unfavorable bitter taste. These shortcomings were improved by the addition of seasoning paste. The calcium and phosphorus contents of the seasoned salt-fermented pearl oyster were 64.2 mg/100 g and 71.6 mg/100 g, respectively, and the calcium content based on phosphorus was a good ratio for absorbing calcium. The total amino acid content of the seasoned and salt-fermented pearl oyster was 7,054 mg/100 g and the major amino acids ware aspartic acid (555.1 mg/100 g), glutamic acid (1,131.2 mg/100 g), alanine (658.2 mg/100 g), and lysine (695.5 mg/100 g). The seasoned salt-fermented pearl oyster, along with angiotensin I converting enzyme (ACE) inhibitory activity (98.3%), also showed a recognizable level (87.5%) of anti-oxidative activity.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.6
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• pp.520-526
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• 2007

The accuracy of model surgery is one of important factors which can influence the outcome of orthognathic surgery. To evaluate the accuracy of digitalized model surgery, we tried the model surgery on a software after transferring the mounted model block into a digital model, and compared the results with that of classical manual model surgery. We could get the following results, which can be used as good baseline analysis for the clinical application. 1. We made the 3D scanning of dental model blocks, and mounted on a software. And we performed the model surgery according to the previously arranged surgical plans, and let the rapid prototyping machine produce the surgical wafer. All through these process, we could confirm that the digital model surgery is feasible without difficulties. 2. The digital model surgery group (Group 2) showed a mean error of $0.0{\sim}0.1mm$ for moving the maxillary model block to the target position. And Group 1, which was done by manual model surgery, presented a mean error of $0.1{\sim}1.2mm$, which is definitely greater than those of Group 2. 3. Remounted maxillary model block with the wafers produced by digital model surgery from Group 2 showed the less mean error (0.2 to 0.4 mm) than that produced by manual model surgery in Group 1 (0.3 to 1.4 mm). From these results, we could confirm that the digital model surgery in Group 2 presented less error than manual model surgery of Group 1. And the model surgery by digital manipulation is expected to have less influence from the individual variation or degree of expertness. So the increased accuracy and enhanced manipulability will serve the digital model surgery as the good candidate for the improvement and replacement of the classical model surgery, if careful preparation works for the clinical adjustment is accompanied.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.2
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• pp.71-78
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• 2014

There is a big risk of bullet impact because military rotorcraft is run in the battle environment. Due to the bullet impact, the rapid increase of the internal pressure can cause the internal explosion or fire of fuel cell. It can be a deadly damage on the survivability of crews. Then, fuel cell of military rotorcraft should be designed taking into account the extreme situation. As the design factor of fuel cell, the internal fluid pressure, structural stress and bullet kinetic energy can be considered. The verification test by real object is the best way to obtain these design data. But, it is a big burden due to huge cost and long-term preparation efforts and the failure of verification test can result in serious delay of a entire development plan. Thus, at the early design stage, the various numerical simulations test is needed to reduce the risk of trial-and-error together with prediction of the design data. In the present study, the bullet impact numerical simulation based on SPH(smoothed particle hydrodynamic) is conducted with the commercial package, LS-DYNA. Then, the resulting equivalent stress, internal pressure and bullet's kinetic energy are evaluated in detail to examine the possibility to obtain the configuration design data of the fuel cell.

• Polymer(Korea)
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• v.36 no.4
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• pp.486-493
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• 2012

Photocurable sulfonated polyimide (SPI) polyelectrolyte containing chalcone group was prepared and fabricated on an alumina electrode pretreated with chalcone-containing silane-coupling agent. SPI films with bis(tetramethyl)ammonium 2,2'-benzidinedisulfonate ($Me_4N$-BDS)/4,4'-diaminochalcone (DAC)/pyromellitic dianhydride (PA)= 90/10/100 possessed very linear response(Y = -0.04528X+7.69446, $R^2=0.99675$) and showed resistance changing from 4.48 to $2.1k{\Omega}$ between 20 and 95 %RH. The response time for absorption and desorption measurements between 33 and 94 %RH% was about 79 s, which affirmed the high efficiency of crosslinked SPI film for rapid detection of humidity. A negative temperature coefficient showing $-0.49%RH/^{\circ}C$ was found and proper temperature compensation should be considered in future applications. Moreover, pretreatment of the substrates with chalcone-containing silane-coupling agent was performed to improve the water durability and the stability of the humidity sensors at a high humidity and a high temperature and long-term stability for 480 h. The crosslinked SPI films anchored to electrode substrate could be a promising material for the fabrication of efficient humidity sensors with superior characteristics compared to the commercially available sensors.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.291-299
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• 2009

To elucidate HtrA3's functional roles in the HtrA3 mediated cellular processes, it is necessary to investigate its biochemical characteristics. In the present study, we constructed the plasmids encoding putative mature HtrA3 proteins (M1-HtrA3 and M2-HtrA3) based on the putative maturation sites of highly homologous HtrA1 and mouse HtrA3. We used the pGEX bacterial expression system to develop a simple and rapid purification for the recombinant HtrA3 protein. Although yields of the mature HtrA3 proteins were slightly low as 10~50 ${\mu}g$/L, the amounts and purity of M1- and M2-HtrA3 were enough to investigate their proteolytic activities. The putative mature HtrA3 proteins have proteolytic activity which could cleave $\beta$-casein as an exogenous substrate. We compared the proteolytic activity between the HtrA family, HtrA1, HtrA2, and HtrA3. The cleavage activity of HtrA3 and HtrA2 were 2 folds higher than that of HtrA1, respectively. Our study provides a method for generating useful reagents to identify natural substrates of HtrA3 in the further studies.

• Archives of design research
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• v.12 no.2
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• pp.31-40
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• 1999

Because of the oocIear family due to the rapid industrialization and the growth of husband and wife bah working, the base of the tradition that the parents, all of the famly or the hamet oonmunity bring up the infants together in the important time to the infarts has been collapsed in Korea. Because working mothers camot bring up their children by themselves they got stressed and housewives do not have the oonfidence in rearing their infants and the fathers are isolated from home and the chikten instead of being exempted from the responsibility of rearing children. Therefore the common and comprehensive rearing system should be made for solving the child-rearing problem. For solving these problems and for the desirable infant-rearing and education, the joint irlart rearing, whidl compensates for the life, education and furthermore the sdlool edJcation through the volur1ary participation, has been prepared altematively. Compared to the traditional rearing system, joint infant-rearing is not only operated by the residents with the dues paid by the union members but also makes children experience the life force of natu"e in themselves. So the preparation of the standard of the suitable environment design for the joint infant-rearing is required which is diffrent from the traditional standard of the environmertal facilities for the traditional rearing system. The concept of joint infant-rearing was defind in this study through the reference and the ooncept of infant edJcation which is in operation suocessfully in Italy and Sweden in order to understand how the ooncept of infant education has changed as the ages have dlanged. And the basic principals for the formation of physical envirorvnert and the spare design and the envirorYnertal oondition for the practical environmental design was also studied through the reference. And after study about the concept. figure and physical environmental suggestion, the concept, standard and element of joint infant-rearing environment design was suggested. And as the final conclusion, the design requirement of the envirorvnertal design for the joint infant-rearing was suggested.

• Korean Journal of Microbiology
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• v.41 no.4
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• pp.275-280
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• 2005

Anthrax is an infectious disease caused by the gram-positive bacterium, Bacillus anthracis. Anthrax toxin is a tripartite toxin comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA is the receptor-binding component, which facilitates the entry of LF or EF onto the cytosol. LF is a zinc-dependent metalloprotease, which is a critical virulence factor in cytotoxicity of infected animals. Therefore, it is of interest to develop its potent inhibitors for the neutralization of anthrax toxin. The first step to identify the inhibitors is the development of a rapid, sensitive, and simple assay method with a high-throughput ability. Much efforts have been concentrated on the preparation of powerful assays and on the screening of inhibitors using these system. In the present study, we have tried to construct anthrax lethal factor in yeast expression system to prepare cell-based high-throughput assay system. Here, we have shown the results covering the construction of a new vector system, subcloning of LF gene, and the expression of target gene. Our results are first trial to express LF gene in eukaryote and provide the basic steps in design of cell-based assay system.

• Journal of Animal Environmental Science
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• v.15 no.2
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• pp.107-114
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• 2009

Civil appeals on odor from swine barn have been increasing. This study was conducted to compile basic data of odor from swine barns as civil appeals on odor have been increasing. Odor was monitored from two gestating sow barns and the boundary of those barns in Suwon and Hwaseong city from June to November, 2008. Mean ammonia concentration measured at the boundary of A piggery in Suwon city was 0.9 ppm with occasional breaks of permissible limit (1.0 ppm). Mean concentrations of hydrogen sulfide and methyl mercaptan were 0.37 ppb and 0.01 ppb, which were lower than permissible limits of 20 ppb and 2 ppb, respectively. Mean ammonia concentration was 3.22 ppm and was maintained under 5.0 ppb. Ammonia concentration had increased since November when the barns were closed. Hydrogen sulfide and methyl mercaptan were lower than the permissible limits. Mean ammonia concentration measured at the boundary of B piggery in Hwaseong city was 16.3 ppm and steep increase of concentration was monitored after October when the barn was sealed up. Hydrogen sulfide and methyl mercaptan were 26.66 ppb and 7.45 ppb, respectively. In appropriate ventilation and raising environment would cause high ammonia concentration in swine barns. Emitted ammonia from barns and composting facilities were mixed, which would cause higher ammonia concentration on the boundary of barns. Hence improvement of raising environment, rapid manure treatment, and correct use of microbial preparation would be needed.

• Korean journal of food and cookery science
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• v.19 no.3
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• pp.339-353
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• 2003

The rapid increase in food borne illness outbreaks in Korea has been one of the major threats to the Nation's Health. Foodservice establishments have been identified as the major place for these outbreaks, mainly due to the lack of sanitary management and sanitary facility management practices. The purposes of the study were to develop hospital foodservice facility evaluation tools, based on the general HACCP-based standards and guidelines, for hospital food service establishments, to ensure the safety of these foodservices and to reduce the risk of food home illness. The scope of this study included: 1) an assessment of the current foodservice sanitation practices and managements for 6 general hospitals, with more than 400 beds, and 3 general hospitals, with less than 400 beds; 2) the development of foodservice establishments sanitation evaluation tools and sanitation standards, based on the HACCP system. The survey data showed varied results between the hospitals surveyed. Most of the hospital foodservice operations had many problems with ventilation and the plumbing. The total dimensional mean scores for the hospitals with more than 400 beds and less than 400 beds were 31.5 and 27.0, respectively. The highest dimension scores were for the water supply facility and lighting, with the lowest for insect and rodent control and toxic materials management. The levels of the mean scores were very low, especially for the general hospitals with less than 400 beds. These low mean scores may have arisen from critical problems within the hospital foodservice operations. The most needed facility management items for improvement were: storage shelf should be spaced 6 inches from the floor and walls, the use of three compartment sinks, utility sinks and cleaning facilities, with a floor drain for cleaning mops or liquid wastes, a ventilation hood designed to prevent dripping onto food, cooking facilities should be disassembled for washing and sanitizing, a separated hand washing sink and a sanitized food board for each area should be provided, all toxic material must have warning labels attached, and be stored in an area away from food preparation under padlock. The evaluation tool consisted of 14 dimensions, with 65 check-off items. The results of this study will provide basic facilities' guidelines to regulators, or foodservice industry personnel, wishing to build, or expend, and establish an efficient flow of food. As a result, food borne illnesses will be effectively prevented, and the Nation's health will be promoted for the development of their own sanitation standards, with a checklist for the safe production of foods.

• Korean journal of food and cookery science
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• v.24 no.5
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• pp.573-579
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• 2008

In this study, kimchi containing Styela clava (Korean name: miduduk) was prepared, and the quality of the prepared kimchi was evaluated during 4 weeks of fermentation at $4^{\circ}C$. S. clava was added to salted cabbage at concentrations of 0, 1, 2, and 3%(w/w). The quality characteristics of the kimchi were determined by measuring pH, titratable acidity, salinity, color, and microbial amounts. All kimchi evidenced a rapid decrease in pH until 2 weeks, and then a gradual decline thereafter. Titratable acidity increased gradually until 2 weeks, then sharply increased thereafter. Salinity increased until 3 weeks. We noted only slight overall color differences between the kimchi samples. Total microbial and lactic acid bacterial counts achieved maximum levels at 3 weeks, and the kimchi to which 1 and 2% S. clava was added evidenced values higher than that of the controls. In our sensory evaluations, the kimchi to which 2% S. clava was added was scored highest in terms of color, flavor, and overall acceptance.