• 한국측량학회지
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• 제36권2호
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• pp.51-58
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• 2018

최근, 다양한 위성센서가 개발되면서 다시기 위성영상의 취득이 용이해지고 있다. 이에 따라, 재난/재해, 국토모니터링 등과 같은 활용분야에 다시기 위성영상을 적용하기 위한 변화탐지 기법에 대한 연구들이 수행되고 있다. 특히, 빠른 시간 내에 변화지역의 추출이 가능한 무감독 변화탐지 기법의 개발과 관련된 연구들이 수행되고 있지만, 계절적 변화 등과 같은 방사적 차이로 인해 오탐지가 발생하는 단점이 있다. 따라서, 본 연구에서는 무감독 변화탐지 기법 중의 하나인 $S^2CVA$ 기법을 적용하여 생성한 변화방향 벡터를 이용하여 계절적 영향으로 인한 오탐지를 감소시키고자 하였다. 이를 위하여, 동일한 계절을 가지는 RapidEye 위성영상과 다른 계절에 촬영된 RapidEye 위성 영상에 $S^2CVA$ 기법을 적용하였으며, $S^2CVA$의 변화방향벡터가 계절적 영향에 따른 오탐지를 제거할 수 있는지를 분석하였다. 정량적 평가를 위해 변화탐지 결과의 ROC 곡선과 AUC 분석을 통해 기존의 방법에 비해 변화탐지 성능이 향상된 것을 확인하였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.698-701
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• 2006

Remotely sensed data have been utilized efficiently for damage detection immediately after the natural disaster since they provide valuable information on land cover change due to spatial synchronization and multitemporal observation over large areas. Damage information obtained at an early stage is important for rapid emergency response and recovery works. Many useful techniques to analyze the characteristics of the pre- and post-event satellite images in large-scale damage detection have been successfully investigated for emergency management. Since high-resolution satellite images provide a wealth of information on damage occurred in urban areas, they are successfully utilized for damage detection in urban areas. In this research, a method to perform automated damage detection is proposed based on the differences of the textural characteristics in pre- and post- high resolution satellite images.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1629-1632
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• 2008

Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time detection of microorganisms is introduced. We fabricated a new impedimetric biosensor by integrating solid media and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid-medium biosensor could monitor bacterial growth in situ with a detection time of ${\sim}4$ hrs. Our data indicate that the solid-medium biosensor is useful for detecting airborne microorganisms, thereby providing a new analytical tool for impedance microbiology.

• 한국축산식품학회지
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• 제32권6호
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• pp.706-712
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• 2012

The present study was aimed to produce a chicken polyclonal antibody against Cronobacter muytjensii and to develop an immunoassay for its detection. Purification of anti-C. muytjensii IgY from egg yolk was accomplished using various methods such as water dilution and salt precipitation. As a result, sodium dodecyl sulfate-polyacrylamide gel electrophoresis produced two bands around 30 and 66 kDa, corresponding to a light and a heavy chain, respectively. Indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was performed to determine the effectiveness of the chicken IgY against C. muytjensii. The optimum conditions for detecting C. muytjensii by indirect ELISA and checkerboard titration of the antigen revealed an optimum average absorbance at the concentration of 18 ${\mu}g/mL$, having ca. $10^8$ coated cells per well. The anti-C. muytjensii IgY antibody had high specificity for C. muytjensii and low cross-reactivity with other tested pathogens. In this assay, no cross-reactivity was observed with the other genera of pathogenic bacteria including Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Bacillus cereus, Enterobacter aerogenes, Salmonella Enteritidis and Listeria monocytogenes. In addition, detection of C. muytjensii in infant formula powder showed a low matrix effect on the detection curve of IC-ELISA for C. muytjensii, with similar detection limit of $10^5$ CFU/mL as shown in standard curve. These findings demonstrate that the developed method is able to detect C. muytjensii in infant formula powder. Due to the stable antibody supply without sacrificing animals, this IgY can have wide applications for the rapid and accurate detection of C. muytjensii in dairy foods samples.

• 한국동물위생학회지
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• 제45권1호
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• pp.19-30
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• 2022

In this study, a simple loop-mediated isothermal amplification (LAMP) combined with visual detection method (vLAMP) assay was developed for the rapid and specific detection of African swine fever virus (ASFV), overcoming the shortcomings of previously described LAMP assays that require additional detection steps or pose a cross-contamination risk. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubation for 40 min at 62℃. The assay specifically amplified ASFV DNA and no other viral nucleic acids. The limit of detection of the assay was <50 DNA copies/reaction, which was ten times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). For clinical evaluation, the ASFV detection rate of vLAMP was higher than cPCR and comparable to OIE-recommended qPCR, showing 100% concordance, with a κ value (95% confidence interval) of 1 (1.00~1.00). Considering the advantages of high sensitivity and specificity, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vLAMP assay will be a valuable tool for detecting ASFV from clinical samples, even in resource-limited laboratories.

• 한국양봉학회지
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• 제32권3호
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• pp.171-180
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• 2017

Slow Bee Paralysis Virus (SBPV)는 꿀벌 그리고 뒤영벌의 병원성 바이러스로써, 벌의 앞발을 마비시킴으로 폐사를 야기한다. 본 연구는, 뒤영벌에서 빠르게 SBPV 검출하고자 SBPV 특이 초고속 PCR법을 개발한 것을 보고하는 것이다. SBPV 특이 초고속 PCR은 제반 조건을 최적화한 후, $1.0{\times}10^8$ 분자의 SBPV 특이 유전자의 존재를 3분 35초 만에 인식할 수 있었으며, 최소 $1.0{\times}10^1$ 분자의 DNA까지 정량적으로 측정할 수 있었다. 한편, 국내외에서 생산된 뒤영벌을 시료로 SBPV 특이 초고속 역전사 PCR을 시행하였으며, 수입된 뒤영벌과 국내생산 뒤영벌 1종에서 SBPV가 존재함을 보일 수 있었다. 국내 처음으로 뒤영벌에서의 SBPV의 존재를 확인한 것이다. SBPV 특이 유전자 초고속 역전사 PCR법은 실험실 내에서 뿐만 아니라 현장에서 정량적 검출을 하기에 유용한 방법이 될 것이며 더 나아가 뒤영벌 수출입을 위한 검역 기준을 이용하여 집단적 발병 방지에 기여하기를 기대한다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.143.2-143
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• 2003

A rapid and sensitive assay for the specific detection of plant viroids using reverse transcription-polymerase chain reaction(RT-PCR) has been developed already. The nested RT-PCR assay cloud be applied for the detection of apple scar skin viroid(ASSVd) from young leaves and other tissues. ASSVd has central conserved region(CCR), terminal left(T$\sub$L/) and terminal right(T$\sub$R/) domain. Primers were designed from these regions. Primer sets were successfully applicable for the amplification of full length or partial region of ASSVd by nested RT-PCR. Nested RT-PCR assay was more sensitive and accurate method to detect ASSVd from young trees during the early time of apple cultivation.

• Journal of electromagnetic engineering and science
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• 제5권1호
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• pp.26-30
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• 2005

It is important to know phase offsets of PN(Pseudo Noise) sequences in spread spectrum communications since the acquisition is equivalent to making a phase offset between a receiving PN sequence and a PN sequence of local PN generator be identical. In this paper, a phase offset enumeration method for PN sequences with error detection, and its application to the synchronization are proposed. The phase offset enumeration for an n-tuple PN sequence and its error detection are performed when one period of the sequence is received. Once the phase offset of the receiving sequence is calculated, we can easily accomplish the synchronization by initializing shift registers of a local PN generator according to the phase offset value. The mean acquisition time performance of the proposed scheme was derived analytically. Since this synchronization scheme can be realized by using simple circuit and acquires very rapid acquisition in high SNR but shows performance degradation in low SNR, it can be especially useful in indoor and office environments.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1782-1786
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• 2015

A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.

• Nuclear Engineering and Technology
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• 제53권10호
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• pp.3256-3263
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• 2021

The main steam line break accident is an essential initiating event of the pressurized water reactor. In present work, the fuzzy set theory and the signal-based fault detection method has been used to detect the occurrence and diagnosis of the location and break area for the small scale MSLB. The models are validated by the AP1000 accident simulator based on MAAP5. From the test results it can be seen that the proposed approach has a rapid and proper response on accident detection and location diagnosis. The method proposed to evaluate the break area shows good performances for small scale MSLB with the relative deviation within ±3%.