• 한국콘텐츠학회논문지
• /
• 제12권3호
• /
• pp.161-170
• /
• 2012

본 연구는 선행연구에서 도출된 무용영재성 구성요인을 바탕으로 무용영재를 판별할 수 있는 구조화된 평가도구를 개발하는 것이 목적이다. 무용영재의 잠재성을 대변할 수 있는 적합한 문항을 개발하기 위하여 무용영재성 구성요인인 신체적 조건, 동작수행능력, 표현 및 창의성, 무용태도 요인을 바탕으로 초기문항을 개발하였다. 1차 기초조사는 무용을 전공자와 직업무용단 단원 등을 조사 대상자로 선정하여 총 206명에게 실시하였고, 설문조사 결과자료를 바탕으로 기술통계 분석과 신뢰도 분석, 그리고 탐색적 요인분석을 실시하였다. 그 결과, 4요인(신체적요인, 표현 및 창의성, 무용태도, 동작수행능력)과 20개의 문항이 선정되었다. 선정된 모형(4개의 구인과 20개의 문항)의 적합도를 알아보기 위하여 1차 설문조사 대상과는 다른 무용전공생과 직업무용단 단원 등을 선정하여 총 292명에게 2차 기초조사를 실시하였다. 설문조사 결과자료를 바탕으로 확인적 요인분석을 실시한 결과, 모든 적합도 지수가 기준치에 만족하는 것으로 나타났다. 본 결과를 통해 4요인의 20개인 문항에 대한 모형은 적합한 것으로 판명되어 최종으로 채택하였다.

• Journal of Microbiology and Biotechnology
• /
• 제15권5호
• /
• pp.1047-1053
• /
• 2005

Cyclosporin A binding protein type-19 kDa peptidyl-prolyl cis/trans isomerase (PPIases, EC 5.2.1.8) of Euglena gracilis was purified and some of its biochemical characters were elucidated. Purification of the PPIase was achieved by employing a series of steps involving ammonium sulfate precipitation, Superdex G-75 gel filtration chromatography, Mono­Q anion and Mono-S cation exchange chromatographies, and Superdex S-200 gel filtration chromatography on FPLC. Purified PPIase had a specific activity of 8,250 units/mg, showing a 27-fold increase compared with that of cell-free extract of Euglena gracilis. The enzyme consisted of a single polypeptide chain with a molecular mass of 19 kDa. It showed high substrate specificity to succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, and $k_{car}/K_{m}$, for this substrate was found to be $61.19{\times}10^5/sec$. The isomer distributions were investigated at an equilibrium of seven different peptide substrates, varying Xaa in Suc-Ala-Xaa-Pro-Phe-p-nitroanilide in dimethylsulfoxide. The cis/trans equilibrium constants were estimated to be from 0.14 (Ile) to 0.63 (Gly), which correspond to $12.00\%\;to\;38.52\%$ of the cis population, respectively, under experimental condition. The enzyme was highly sensitive to the immunosuppressive ligand cyclosporin A, but not to other immunosuppressants such as FK506 and rapamycin. Thus, it appears to belong to the class of cyclophilin.

• 통합자연과학논문집
• /
• 제8권4호
• /
• pp.285-292
• /
• 2015

LIM kinases belong to the serine/Threonine kinase family. The members of the LIM kinase (LIMK) family include LIMK 1 and 2 which are involved in the regulation of actin polymerisation and microtubule disassembly. LIMK1 was shown to be involved in cancer metastasis, while LIMK2 activation promotes cells cycle progression. Since LIMK2 plays a vital role in many disease conditions such as pulmonary hypertension, cancer and viral diseases, and till date there are not much selective inhibitors been reported, LIMK2 becomes an interesting therapeutic target among the kinases. 3D QSAR study was carried out on a series of pyrrolopyrimidines based derivatives as LIMK2 inhibitors. A reasonable CoMFA ($q^2$=0.888; ONC=3; $r^2$=0.974) with good statistical values was developed. The developed model was validated using 1000 runs of boostrapping and was found to be predictable. The results of CoMFA contour map analysis suggested that the bulky substitution at $R_4$ and $R_5$ position are highly desirable to increase the activity. Similarly, positive substitution at $R_3$ position is also required to increase the activity. It is also noted that bulky substitution at $R_1$ position must be avoided. Our results could provide valuable information to enhance the activity of the LIMK2 inhibitors and to design potent pyrrolopyrimidines derivatives.

• 한국전자파학회논문지
• /
• 제20권9호
• /
• pp.961-966
• /
• 2009

본 논문에서는 메타 구조 Broadside Coupled 나선형 공진기(BC-DSRs)를 이용하여 전압 제어 발진기의 위상 잡음 특성을 줄이기 위한 새로운 구조를 제안하였다. 이러한 특성 실현을 위하여 연속된 나선형 구조를 신호면과 그라운드 면에 각각 적용하였다. 일반적인 전압 제어 발진기와 비교하였을 때, 본 논문에서 제안한 VCO는 더 큰 결합 계수를 가지며, 이로 인하여 얻을 수 있는 더 높은 Q값을 통하여 전압 제어 발진기의 위상 잡음을 줄일 수 있다. 1.8 V의 공급 전력을 갖는 전압 제어 발진기는 주파수 조절 범위, $5.749{\sim}5.853\;GHz$에서 $-121{\sim}-117.16\;dBc$/Hz @ 100 kHz의 위상 잡음 특성을 갖는다. 또한 전압 제어 발진기의 Figure Of Merit(FOM)은 동일한 주파수 조절 범위에서 $-198.45{\sim}-194.77\;dBc$/Hz @ 100 kHz의 특성을 보였다.

• 자원환경지질
• /
• 제11권2호
• /
• pp.47-57
• /
• 1978

The study focused on the petrology and petrochemistry of the so called "Ganghwa syenitic rocks" which intruded into metasediment of basement in southeastern part of Ganghwa Island. The geologic sequence of the mapped area was shown in table 1, 10 model analyses and 7 chemical analyses on the rock samples taken from the Ganghwa syenitic rocks and Manisan granite have been used to discuss the nomenclature of the rocks and petrological relationship between rock types. The petrograpical and petrochemical features based on, the analyses are as follows: 1) Ganghwa syenitic rocks consist of Ganghwa alkali syenite and Ganghwa diorite porphyry which based on the classification of the subcommision on systematics of igneous of IGUS. Ganghwa diorite porphyry which occured as dike forms are intruded into Ganghwa alkali syenite. The rock forming minerals of Ganghwa alkali syenite are composed of perthite, plagioclase, quartz, hornblend and chlorite in major, and zircon, apatite, sericite and magnetite in minor. Ganghwa diorite porphyries consist of plagioclase, biotite, hornblend, orthoclase and chlorite, with, porphyritic texture. 2) In silica-oxides variation (Fig. 2) and AMF diagram (Fig_ 3), the Ganghwa alkali syenite is similar to the trend of Daly's average basalt-andesite-dacite-rhyolite than Skaergaard which shows the trend of the fractional crystallization of magma, and equivalent to the alkali rock series by Peacock. 3) The general trend of data points shift to plagioclase, and are superimposed on the alkali rich terminal part of the granodiorite province of SW Finland in normative Q-Kf-Pl(Fig. 4) and Or-Ab-An diagram respectively. The above-mentioned evidences suggested that the Ganghwa syenitic rocks are the differential products resulted by assimilation of intermediated magma and metasedment rock under relatively rapid cooling condition.

• ALGAE
• /
• 제31권3호
• /
• pp.205-217
• /
• 2016

Freshwater red tides due to dinoflagellates have caused spectacular and regular “summer reddening” in recent years in Baishihai Lake, a temperate, meromictic, meso- or oligotrophic, high-altitude, landslide-dammed, deep lake located at the eastern edge of Tibetan Plateau in China. Based on morphological and molecular analyses, the causative organism has been identified as a new woloszynskioid dinoflagellate, Tovellia diexiensis Q. Zhang et G. X. Liu sp. nov. The vegetative cells are 20-32 μm long and 16-24 μm wide. They have a hemispherical episome and a broadly rounded hyposome with a short characteristic antapical spine. Usually cells are bright red due to the presence of numerous red-pigmented bodies, which often masked the yellowish green discoid chloroplasts. The amphiesma of motile cells comprise mainly quadrilateral, pentagonal or hexagonal thin plates, arranged in 4-5 latitudinal series on the episome, 1 in the cingulum and 4 on the hyposome. Molecular phylogenies based on small subunit ribosomal DNA and large subunit ribosomal DNA (LSU) indicate T. diexiensis from Baishihai Lake to belong to the family Tovelliaceae, which was monophyletic in our LSU phylogenies. During the bloom-forming period in 2005, cell density of T. diexiensis reached 9.15 × 10⁵ cells L⁻¹. Astaxanthin and its diester were found to be the major pigments in T. diexiensis, resulting in a characteristic blood-red color of the water in Baishihai Lake.

• Environmental Engineering Research
• /
• 제15권4호
• /
• pp.207-213
• /
• 2010

This study investigated the potential of using bottom ash to be used as an adsorbent for the removal of lead (Pb) from aqueous solutions. The physical and chemical characteristics of bottom ash were determined, with a series of leaching and adsorption experiments performed to evaluate the suitability of bottom ash as an adsorbent material. Trace elements were present, such as silicon and aluminum, indicating that the material had a good adsorption capacity. All heavy metals leached during the Korea standard leaching test (KSLT) passed the regulatory limits for safe disposal, while batch adsorption experiments showed that bottom ash was capable of adsorbing Pb (experimental $q_e$ = 0.05 mg/g), wherein the adsorption rate increased with decreasing particle size. The adsorption data were then fitted to kinetic models, including Lagergren first-order and Pseudo-second order, as well as the Elovich equation, and isotherm models, including the Langmuir, Freundlich and Dubinin-Radushkevich isotherms. The results showed that pseudo-second order kinetics was the most suitable model for describing the kinetic adsorption, while the Freundlich isotherm best represented the equilibrium sorption onto bottom ash. The maximum sorption capacity and energy of adsorption of bottom ash were 0.315 mg/g and 7.01 KJ/mol, respectively.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 1998년도 Proceedings of International Symposium on Remote Sensing
• /
• pp.236-241
• /
• 1998

The NOAA AVHRR remote sense SST data, collected by the National Fisheries Research and Development Institute (NFRDI), are analyzed in order to understand the spatial and temporal distributions of SST in the seas adjacent to Korea. Our study is based on 10-day SST images during last 7 years (1991-1997). For a time series analysis of multiple 557 images, all of images must be aligned exactly at the same position by adjusting the scales and positions of each SST image. We devised an algorithm which yields automatic detections of cloud pixels from multiple SST images. The cloud detection algorithm is based on a physical constraint that SST anomalies in the ocean do not exceed certain limits (we used $\pm$ 3$^{\circ}C$ as a criterion of SST anomalies). The remote sense SST data are tuned by comparing remote sense data with observed SST at coastal stations. Seasonal variations of SST are studied by harmonic fit of SST normals at each pixel. The SST anomalies are studied by statistical method. We found that the SST anomalies are rather persistent with time scales between 1 and 2 months. Utilizing the persistency of SST anomalies, we devised an algorithm for a prediction of future SST Model fit of SST anomalies to the Markov process model yields that autoregression coefficients of SST anomalies during a time elapse of 10 days are between 0.5 and 0.7. We plan to improve our algorithms of automatic cloud pixel detection and prediction of future SST. Our algorithm is expected to be incorporated to the operational real time service of SST around Korea.

• Journal of Microbiology and Biotechnology
• /
• 제15권2호
• /
• pp.395-402
• /
• 2005

Biologically active recombinant human follicle stimulating hormone (rhFSH) was produced in Chinese hamster ovary cells and purified by a series of chromatographic steps. The chromatographic steps included anion-exchange chromatography (DEAE Sepharose F/F, Q Sepharose F/F), hydrophobic interaction chromatography (Source 15 PHE), and hydroxyapatite chromatography (Macro-Prep ceramic hydroxyapatite type I). A distinctive step of the purification process developed was the use of ZnCl$_2$ for the removal of non-glycosylated or lowly-glycosylated FSH and impurities through co-precipitation with Zn$^{2+}$. Purified rhFSH was identified and characterized by several physicochemical and biological methods such as gel electrophoresis, high-performance liquid chromatography, amino acid analysis, carbohydrate analysis, and biological activity. The overall yield of the purification was ~$30\%$. The rhFSH preparation obtained showed high purity (>$99\%$) and high in vivo potency (>16,000 IU/mg). Carbohydrate analysis suggested that the purified rhFSH contained approximately $40\%$ (w/w) carbohydrate with di­or tri-antennary structure on average, which is somewhat more heavily sialylated than commercially available rhFSH. In conclusion, the results of these analyses established an identity of the purified rhFSH with natural FSH from human pituitary glands, and furthermore, the purified rhFSH preparation showed higher in vivo potency and was slightly more heavily sialylated than commercially available rhFSH.

• 설비공학논문집
• /
• 제8권1호
• /
• pp.1-12
• /
• 1996

In lots of application to heat exchanger systems, closed two-phase thermosyphons are tilted from a horizontal. If the tilt angle, especially, is less than 30$^{\circ}$, the operational performances of thermosyphon are highly dependent on tilt angle. The present study was conducted to better understand such operational behaviors as mech-anni는 of phase change, and flow patterns inside a tilted thermosyphon. For experiment, an ethanol thermosyphon with a 35% of fill charge rate was designed and manufactured, using a copper tube with a diameter 19mm and a length 1500mm. Through a series of test, the tilt angle was kept constant at each of 4 different values in the range 10~25deg. and the heat supply to the evaporator was stepwisely increased up to 30㎾/$m^2$. When a steady state was established to the thermosyphon for each step of thermal loads, the wall temperature distribution and vapor temperature at the condenser were measured. The wall temperature distributions demonstrated a formation of dry patch in the top end zone of the evaporator, with a values of temperature 20~4$0^{\circ}C$ higher than the wetted surface for a moderate heat flux q≒20㎾/$m^2$. Inspite of the presence of hot dry patch, however, the mean values of boiling heat transfer coefficient at the evaporator wall were still in a good agreement with those predicted by Rohsenow's formula, which was based on nucleate boiling. For the condenser, the wall temperatures were practically uniform, and the measured values of condensation heat transfer coefficient were 1.7 times higher than the predicted values obtained from Nusselt's film condensation theory on tilted plate. Using those two expressions, a correlation was formulated as a function of heat flux and tilt angle, to determine the total thermal resistance of a tilted thermosyphon. The correlation formula showed a good agreement with the experimental data within 20%.