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Identification of Genes Involved in Primordial-primary Follicle Transition by Suppression Subtractive Hybridization

  • Park, Chang-Eun;Yoon, Se-Jin;Jeon, Eun-Hyun;Kim, Young-Hoon;Lee, Sook-Hwan;Lee, Kyung-Ah
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.98-98
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    • 2002
  • Recruitment of primordial follicles(PMF) is crucial for female fertility. however, factors and mechanisms that regulate this process is poorly understood. The present study was conducted to obtain an inclusive view of the gene expression and to identify novel factors and their pathways of regulating PMF arrest and/or growth initiation. Ovaries from one-day neonatal(consists of oocyte and PMF) and five-day old(consists of PMF and primary follicles, PRIF) mice were collected, either total RNA or mRNA was isolated, and suppression subtractive hybridization(SSH) was used to isolate and clone genes that differentially expressed in day 1 and day 5 ovaries. Confirmation that some of these genes are differentially expressed in PMF and/or in PRIF was accomplished by using laser captured microdissection(LCM), RT-PCR. in situ hybridization(ISH) and/or immunohistochemistry(IHC). In toto, 357 clones were sequenced and analyzed by BLAST and RIKEN program. Sequences of 330 clones significantly matched database entries while 27 clones were novel. Forty-two and 47 different genes were identified as differentially expressed in day 1 and day 5 ovaries, respectively, while 7 genes were expressed in both stages of ovaries. Day 5-subtracted library included several genes known as markers far growing follicles, such as ZP2, MATER, and fetuin. Among the genes with assigned functions, 23.8% was associated with cell cycle/apoptosis regulation, 7.1% with cellular structure, 11.9% with metabolism, 26.2% with signal transduction, and 31.0% with gene/protein expression in day 1; while 10.6%, 17.0%, 23.5%, 25.5%, and 23.4% in day 5, respectively. Genes such as GDF-8, Lats2, Septin2, and Weel were the highly expressed genes in PMF, while HSP84, Laminin2, MATER, MTi7, PTP, and Wrn were highly expressed genes in PRIF. We have successfully discovered list of genes expressed in day 1 and day 5 ovaries and confirmed that some of them are differentially expressed in PMF and/or PRIF. Gene expression profile from the present study would provide insight for the future study on the mechanism(s) involved in primordial-primary follicular transition. This work was Supported by Korean Health 21 RND Project, Ministry of Health and Welfare, Korea (01-PJ10-PG6-01GN13-0002).

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Investigating Science-Talented Students' Understandings and Meaning Generation about the Earth Systems Based on Their Geological Field Trip Reports (야외지질답사 보고서에 나타난 과학영재학생들의 지구계 이해와 지구계 의미 생성 탐색)

  • Yu, Eun-Jeong;Lee, Sun-Kyung;Kim, Chan-Jong
    • Journal of the Korean earth science society
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    • v.28 no.6
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    • pp.673-685
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    • 2007
  • The purpose of this study was to investigate Earth Systems Understandings (Mayer, 1991) and Earth Systems meaning generation reported by science-talented students who participated in a geological field trip. The eight (4 female and 4 male students) field trip reports were randomly selected among all the reports written by twenty eighth-grade students who joined Shiwha-Lake field trip in Korea. The three-step program, including preparation, field trip, and summary, was provided to the students in order to facilitate meaningful learning through outdoor teaming activities. Seven Earth Systems Understandings and thematic types (Keys, 1999) were used to analyze the reports. The results of this study indicated thai aesthetic views and stewardship toward the Earth, which were the most distinguishing characteristics in Earth Systems Education, were reflected on most of the reports. The results also showed that the students tried to represent their understandings in such a type as meaning extension, meaning enhancement, or meaning elaboration. Overall, many students used 'knowledge-telling' process with a long list of observations and facts, whereas a few students used higher-order 'knowledge-transforming' process by coordinating their findings with interpretations and reasoning in their writings.

Comparison Between South and North Korean Terminologies in Child and Family Domains of Family Life Education (남북한 가정생활교육 관련용어 비교분석 - 아동·가족분야를 중심으로 -)

  • Lim, JungHa;Chung, SoonHwa;Song, Jieun
    • Journal of Korean Home Economics Education Association
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    • v.28 no.2
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    • pp.61-78
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    • 2016
  • The purpose of this study was to examine the differences in terminologies used in child and family domains of family life education in South and North Korea. The terminologies relevant to child and family domains in North Korea were selected from various sources including dictionaries that are developed to compare South-North Korean languages, reports and websites by ministry of unification in South Korea, magazines and news articles about North Korean daily life. The collected terminologies were analyzed using the content criteria on core concepts, 'development' and 'relations' from the 2015 proclamation of the ministry of education on home economics curriculum. The major differences between the two Koreas were as follows: first, the terms categorized under human development were more divergent compared to the terms categorized under family relations. Specifically, there were big differences in terminologies in the love and marriage section, the life and labor in pregnancy section in human development and the child caring section in family relations. Second, dissimilar terms were more frequently appeared in content area of kinship, marriage, and child-rearing. Third, the discrepancies of terms between the two Koreas were brought about primarily by differences in political and social system, language refinement, and transcription techniques. These findings including the terminology list would be a practical resources providing for students to familiarize with the differences in the usage of terms and for teachers to develop a home economics educational program in provision of reunification of the Koreas.

Analysis of Research Trends in Home Economics Education by Language Network Analysis: Focused on the KCI Journals (2000-2019) (언어 네트워크 분석에 기반 한 가정과교육 연구 동향 분석: 2000-2019년 KCI 등재지를 중심으로)

  • Gham, Kyoung Won;Park, Mi Jeong
    • Journal of Korean Home Economics Education Association
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    • v.32 no.3
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    • pp.179-197
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    • 2020
  • This study analyzed the trends in home economics education research using the language network analysis method, focusing on papers published in the KCI list for 20 years from 2000 to 2019. A total of 501 home economics education papers analyzed through word cloud, centrality analysis, and topic modeling using NetMiner 4.4, and the results are as follows. First, the number of papers in home economics education published in the KCI listing increased gradually to 186 in the 2000s and 315 in the 2010s. The academic journals in which home economics education papers were published have been diversified to 16 in the 2000s and 22 in the 2010s. 60% of all papers were published in the 'Journal of Korean Home Economics Education Association', and since 2018, the number of papers published in the 'Journal of Learner-Centered Curriculum and Instruction' has increased dramatically. Second, in the 2000s and 2010s, home economics education studies published in KCI were categorized into home economics education content analysis, home economics educational program development & application, curriculum analysis, perception survey & direction exploration. In the 2000s, 'Home Economics Teacher' appeared as the main keyword, and a lot of perception survey & direction exploration were conducted. Relatively, the influence of 'development' increased in the 2010s, and many studies were conducted to analyze home economics education contents and develop and apply home economics programs. This study has significance in that it analyzed the research trend of HEE by expanding the analysis target and analysis period of the existing studies.

Effectiveness of Cognitive Behavioral Therapy Techniques for Control of Pain in Lung Cancer Patients: An Integrated Review

  • Phianmongkhol, Yupin;Thongubon, Kannika;Woottiluk, Pakapan
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.14
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    • pp.6033-6038
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    • 2015
  • Background: Experience of lung cancer includes negative impacts on both physical and psychological health. Pain is one of the negative experiences of lung cancer. Cognitive behavioral therapy techniques are often recommended as treatments for lung cancer pain. The objective of this review was to synthesize the evidence on the effectiveness of cognitive behavioral therapy techniques in treating lung cancer pain. This review considered studies that included lung cancer patients who were required to 1) be at least 18 years old; 2) speak and read English or Thai; 3) have a life expectancy of at least two months; 4) experience daily cancer pain requiring an opioid medication; 5) have a positive response to opioid medication; 6) have "average or usual" pain between 4 and 7 on a scale of 0-10 for the day before the clinic visit or for a typical day; and 7) able to participate in a pain evaluation and treatment program. This review considered studies to examine interventions for use in treatment of pain in lung cancer patients, including: biofeedback, cognitive/attentional distraction, imagery, hypnosis, and meditation. Any randomized controlled trials (RCTs) that examined cognitive behavioral therapy techniques for pain specifically in lung cancer patients were included. In the absence of RCTs, quasi-experimental designs were reviewed for possible conclusion in a narrative summary. Outcome measures were pain intensity before and after cognitive behavioural therapy techniques. The search strategy aimed to find both published and unpublished literature. A three-step search was utilised by using identified keywords and text term. An initial limited search of MEDLINE and CINAHL was undertaken followed by analysis of the text words contained in the title and abstract, and of the index terms used to describe the article. A second search using all the identified keywords and index terms was then undertaken across all included databases. Thirdly, the reference list of all identified reports and articles were searched for additional studies. Searches were conducted during January 1991- March 2014 limited to English and Thai languages with no date restriction. Materials and Methods: All studies that met the inclusion criteria were assessed for methodological quality by three reviewers using a standardized critical appraisal tool from the Joanna Briggs Institute (JBI). Three reviewers extracted data independently, using a standardized data extraction tool from the Joanna Briggs Institute (JBI). Ideally for quantitative data meta-analysis was to be conducted where all results were subject to double data entry. Odds ratios (for categorical data) and weighted mean differences (for continuous data) and their 95% confidence intervals were to be calculated for analysis and heterogeneity was to be assessed using the standard Chi-square. Where statistical pooling was not possible the finding were be presented in narrative form. Results: There were no studies located that met the inclusion requirements of this review. There were also no text and opinion pieces that were specific to cognitive behavioral therapy techniques pain and lung cancer patients.Conclusions: There is currently no evidence available to determine the effectiveness of cognitive behavioural therapy techniques for pain in lung cancer patients.

Development of an Editor and Howling Engine for Realtime Software Programmable Logic Controller based on Intelligent Agents (지능적 에이전트에 의한 실시간 소프트웨어 PLC 편집기 및 실행엔진 개발)

  • Cho, Young-In
    • Journal of KIISE:Software and Applications
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    • v.32 no.12
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    • pp.1271-1282
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    • 2005
  • Recently, PC-based control is incredibly developed in the industrial control field, but it is difficult for PLC programming in PC. Therefore, I need to develop the softeware PLC, which support the international PLC programming standard(IECl131-3) and can be applied to diverse control system by using C language. In this paper, I have developed the ISPLC(Intelligent Agent System based Software Programmable Logic Controller). In ISPLC system, LD programmed by a user which is used over $90\%$ among the 5 PLC languages, is converted to IL, which is one of intermediate codes, and IL is converted to the standard C rode which can be used in a commercial editor such as Visual C++. In ISPLC, the detection of logical error in high level programming(C) is more eaier than PLC programming itself The study of code conversion of LD->IL->C is firstly tried in the world as well as KOREA. I developed an execution engine with a good practical application. To show the effectiveness of the developed system, 1 applied it to a practical case, a real time traffic control(RT-TC) system. ISPLC is minimized the error debugging and programming time owing to be supported by windows application program.

Pre-service Teachers' Opinions and Needs on the Physics Education Major Curriculum in College (사범대학 물리교육과의 전공 교육과정에 관한 예비 교사의 의견과 요구)

  • Jo, Kwang-hee
    • Journal of Science Education
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    • v.37 no.2
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    • pp.374-388
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    • 2013
  • The purpose of this study was to investigate pre-service physics teachers' perceptions on the physics education major curriculum. We surveyed 15 junior, and 13 senior college students of physics education major in an university in southern part of Korea. Among them, 24 participants(86 %) took the physics 1 course in high school and 22 participants(79 %) chose the physics 1 in their Korea Scholastic Aptitude Test. The responses showed that the most necessary part in pre-service students' learning was the understanding of high school level physics(36 %), and the understanding of introductory level physics(29 %). In the wish list of courses to be open, high school level physics course was ranked first among seven options by 61 % of respondents. Also, there was some concurrence among respondents in opinion of the necessity for understanding introductory physics. Students felt difficulties in understanding it especially owing to the lack of problem solving skill and comprehension. They added that the sufficient explanation of core concepts should be the first action in the innovative plan. Most participants of pre-service physics teachers hoped to have the revised major curriculum which could help their understanding of high school level or introductory level of physics. However, there was a gap of opinions between the group of students with completion of the high school physics 1 & 2 course and those with non-completion of them. The approach of changing major curriculum with consideration of learners' needs was recommended because the number of students with completion of the high school physics course would probably be decreasing rapidly under these circumstances such as the application of new national curriculum, the reduction of the number of the elective courses in Korea Scholastic Aptitude Test and so on.

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A Study on the Differences in the Dietary-, Health- and Smoking Habits of Young Adult Groups in the Seoul Area (서울지역의 흡연자와 비흡연자의 식이 패턴 및 흡연 행동 특성에 관한 연구)

  • Shin Kyung-Ok;An Eun-Jin;Choi Kyung-Soon;Chung Keun-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.1
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    • pp.54-64
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    • 2006
  • The traditional Korean diet, which is high in rice and vegetables, is fast being replaced by a Western style diet as the standard of living goes up. In addition to this, cigarette smoking has taken on serious proportions, especially amongst those of the younger generation. Because Seoul (and it's surrounding areas) is the hub of Education and Business, it is densely populated and heavily polluted Consequently health-related issues and diseases are starting to resemble those prevalent in Western communities. Historically, stomach cancer was the #1 killer-disease in Korea, but nowadays lung cancer ranks either 1st or 2nd on the list of killer-disease in Korea. This is presumably due to the lethal combination of heavy smoking and pervasive air pollution. Despite these disquieting facts, almost no study had been made of the nutritional patterns and dietary habits of smoking and non-smoking young adults in the Seoul area. Therefore the purpose of this study is to compare the dietary and nutritional patterns of young smokers with those of young non-smokers in and around Seoul. This is done with regards to their eating habits and lifestyles, as well as the dietary components that contribute towards maintaining good health. The results showed that compared to non-smokers, smokers manifested irregular eating patterns and also tended to overindulge on fast- or junk foods. In contrast to this, non-smokers enjoyed a healthier diet, which was high in brown rice, grains, legumes, fresh fruit and vegetables, and 'kimchi'. Amongst the male subjects of this study, it was found that smokers consumed significantly less vitamin $B_2$, calcium, and phosphorous than non-smokers. It was also established that in general, stress-generated smoking habits were formed during the high school years of the younger generation. In conclusion: Because smokers maintain a less healthy diet and life-style, it is to recommended that a 'Health Education Program' be developed which could guide young smokers into adopting better dietary habits and life styles.

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Effects of Variables on Mothers' Choices of Types of Infant Care (영아를 둔 어머니의 어린이집 선택에 영향을 미치는 변인에 관한 연구)

  • Seo, So Jung;Ha, Ji Young
    • Korean Journal of Childcare and Education
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    • v.10 no.2
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    • pp.319-335
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    • 2014
  • The purpose of this study was to examine the effects of variables of interest on mothers' choices of infant care. The variables of interest in this study were categorized into the following three areas: 1) demographic characteristics of mothers and infants, 2) structural and procedual dimensions of infant care facilities, and 3) maternal beliefs on child-rearing. The subjects of this study were mothers whose infants were currently cared by at infant care facilities(n=185) and those who were on the waiting list to use non-maternal infant care(n=53). The main results of this study were as follows. First, mothers of this study preferred to select a type of center-based subsidized infant care over other types of care(family day care) due to quality of the program. Second, the mothers who were not using infant care and cared for their infants at homes reported that there were limitations in their choices of types of infant care due to accessibility and availability of public subsidized infant care facilities, and their child rearing goals were more likely to emphasize learning achievement and maturity than character education or personality. The mothers were more likely to choose a family home care over center-based public subsidized care when they were employed, had younger infants, had longer period of time on waiting lists, and emphasized structural dimensions of infant care than center-based care users. Implications for research and practices were discussed along with the main results of this study.

A Survey on the Housewives' Purchasing Behavior and Needs for Food Safety Information (주부들의 식품안전 관련 구매행동 및 정보요구도 조사)

  • Kim, Kyu-Dong;Lee, Jeung-Yun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.392-398
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    • 2010
  • This study was conducted to investigate housewives' purchase behavior and needs for information on food safety. The data were collected from 275 housewives over the age of 20 living in Seoul and metropolitan areas from March 9 to April 3 in 2009. The result of this study showed that 'checking expiration date' topped with 4.59 in the purchase behavior for food safety followed by 'buying domestics' (4.08) and 'considering food safety' (3.66). The study also showed that only 18.5% of the respondents said that they have an experience of education for food safety, and 'environmental hormones' was the highest with 89.1% in the percentage of correct answer but 'mad cow disease' was the lowest with 25.8%. Also total degree of knowledge for food hazards was an average of 8.56 out of 13. The respondents' degree of information search for food safety was generally low at 2.61 and tv/radio topped the list of information sources with 4.04, followed by newspaper/magazine (3.80), internet (3.44), and family/relatives/friends (3.39). Finally, degree of information needs for food safety was generally high with 3.82. Therefore, government, producers, distributers and academic researchers must provide consumers collective and sufficient information about the food safety and hazards through the diverse information sources. Also they have to play their respective roles in developing and executing feasible education program for consumers.