• 제목/요약/키워드: potato breeding

검색결과 96건 처리시간 0.022초

Identification of the Capsid Protein-binding Region of the SL1(+) RNA Located at the 5' Region of the Potato virus X Genome

  • Cho, Sang-Yun;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • 제28권1호
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    • pp.75-80
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    • 2012
  • $Potato$ $virus$ $X$ (PVX) contains $cis$-acting elements including stem-loop 1 (SL1) RNA at the 5' region; SL1 is conserved among all potexviruses. The SL1 at the positive-sense RNA, SL1(+), is required for PVX RNA replication, cell-to-cell movement, and translation. Previous research demonstrated that SL1(+) RNA also serves as the origin of assembly for encapsidation of PVX RNA. To identify the essential sequences and/or regions for capsid protein (CP) subunit recognition within SL1(+) RNA, we used electrophoretic mobility shift assays (EMSA), UV cross-linking, and yeast three-hybrid analyses. The EMSA and UV cross-linking analyses with PVX CP subunits and RNA transcripts corresponding to the SL1(+) RNA showed that the SL1(+) RNA formed complexes with CP subunits. We also conducted EMSA and yeast three-hybrid analyses with RNAs containing various mutations of SL1(+) RNA elements. These analyses indicated that SL1(+) RNA is required for the interaction with PVX CP and that the RNA sequences located at the loop C and tetra loop of the SL1(+) are crucial for CP binding. These results indicate that, in addition to being important for RNA accumulation, the SL1(+) RNA from the 5' region of the PVX genome is also required for specific binding of PVX CP.

국내 감자바이러스 Y (PVY) 저항성 육성 계통에서 분리한 PVY Mutant의 특성 (Characteristics of Potato Virus Y (PVY) Mutant Isolated from PVY Resistance Breeding Line in Korea)

  • 김재현;금완수;이신호;김정수;전용호;정석훈;정열영;박용학
    • 한국연초학회지
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    • 제28권2호
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    • pp.100-110
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    • 2006
  • A mutant of Potato vims Y (PVY) was occurred in PVY resistance flue-cured tobacco breeding line KF0402 $(TC1146{\times}KF117)$ showing vein necrosis at Suwon in Korea. This isolate, PVY-SWM, was differentiated from other PVY based on biological properties and nucleotide sequence analyses of coat protein gene. PVY-SWM caused typical symptoms on 21 indicator plants as compared to the PVY-TOJC37. Remarkably, the PVY-SWM induced distinctly different symptom of systemic vein necrosis on tobacco cultivars V.SCR, PBD6, TN86, TN90, Virgin A Mutant (VAM), Wislica, NC744, KB108 and KB111, which were reported to have the recessive potyvirus resistance gene va. In RT-PCR assays with specific primers for detection of PVY, a single band of about 800bp in length was produced. The amplified DNA was cloned and the nucleotide sequence was determined. The coat protein gene of PVY-SWM showed 88.4%-99.0% and 92.5%-98.5% identities to the 12 different PVY isolates of Genbank Database at the nucleotide and amino acidi respectively. Multiple alignments as well as cluster dendrograms of PVY-SWM isolate revealed close phylogenetic relationship to the $PVY^{NTN}$ subgroup.

Virus-induced Silencing of the WRKY1 Transcription Factor that Interacts with the SL1 Structure of Potato virus X Leads to Higher Viral RNA Accumulation and Severe Necrotic Symptoms

  • Park, Sang-Ho;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • 제28권1호
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    • pp.40-48
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    • 2012
  • $Potato$ $virus$ $X$ (PVX) replication is precisely regulated by regulatory viral sequences and by viral and/or host proteins. In a previous study, we identified a 54-kDa cellular tobacco protein that bound to a region within the first 46 nucleotides (nt) of the 5' non-translated region (NTR) of the viral genome. Optimal binding was dependent upon the presence of an ACCA sequence at nt 10-13. To identify host factors that bind to 5' NTR elements including AC-rich sequences as well as stemloop 1 (SL1), we used northwestern blotting and matrixassisted laser desorption/ionization time-of-flight mass spectrometry for peptide mass fingerprinting. We screened several host factors that might affect PVX replication and selected a candidate protein, $Nicotiana$ $tabacum$ WRKY transcription factor 1 (NtWRKY1). We used a $Tobacco$ $rattle$ $virus$ (TRV)-based virus-induced gene silencing (VIGS) system to investigate the role of NtWRKY1 in PVX replication. Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation. This result could reflect the balancing of PVX accumulation in a systemic $N.$ $benthamiana$ host to maintain PVX survival and still produce a suitable appearance of mosaic and mottle symptoms. Our results suggest that PVX may recruit the WRKY transcription factor, which binds to the 5' NTR of viral genomic RNA and acts as a key regulator of viral infection.

단일적응 감자 유전자원들의 역병저항성 및 주요 농업형질 평가 (Evaluation of Late Blight Resistance and Agronomic Characteristics of Short-day Adapted Potato Germplasm)

  • 박영은;조현묵;조지홍;조광수;김현준;후안 란데오
    • 원예과학기술지
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    • 제29권5호
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    • pp.474-481
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    • 2011
  • Phytophthora infestans에 의해 유발되는 감자역병은 전 세계의 모든 감자 재배지대에서 발생하는 가장 치명적인 병중의 하나로서 감자역병에 대한 저항성 품종 육성은 매우 중요한 육종 목표로 인식되고 있다. B3C1집단은 국제감자 연구소로부터 도입되었으며 감자역병에 대하여 수평저항성을 가지고 있다. 그러나 이 집단은 페루의 저온단일 환경에서 선발된 계통들로서 고온장일의 국내 환경에 대한 적응성 검정은 이루어진 바 없었다. 따라서 본 연구는 국내의 고온장일 환경 조건에서 B3C1 계통의 역병저항성과 주요 농업 관련 형질들을 평가하여 국내 재배 가능성을 검토해 보고, 앞으로 역병저항성 품종 육성을 위한 육종 소재로써의 가치를 평가하고자 수행되었다. 무방제 포장에서 역병저항성을 평가한 결과 모든 B3C1 계통들은 매우 높은 역병저항성을 나타내어 역병저항성 자원으로 가치가 높은 것으로 확인되었다. 그러나 모든 B3C1 계통에서 공통적으로 나타나는 극도의 만숙성, 괴경 탈립성 불량, 괴경 비대 불량, 그리고 지상부 및 땅속 줄기의 과번무 현상 등은 반드시 개량되어야 할 것으로 판단되었다. B3C1 계통들은 강릉 지역 보다는 대관령 지역에서 더 높은 적응성을 나타내었으며, 최종적으로 20계통 중 LB-8(CIP393077.159)과 LB-11(CIP393371.159) 2계통을 선발하였다. 역병에 강하고 국내의 고온장일 환경에 적응성이 높은 계통을 육성하기 위하여 B3C1 계통들은 국내 육성 계통들과 교배를 실시하였으며, 지속적인 계통 선발이 진행되고 있다.

Establishment of Genetic Transformation System and Introduction of MADS Box Gene in Hot Pepper (Capsicum annuum L.)

  • Lim, Hak-Tae;Zhao, Mei-Ai;Lian, Yu-Ji;Lee, Ji-Young;Eung-Jun park;Chun, Ik-Jo;Yu, Jae-Woong;Kim, Byung-Dong
    • Journal of Plant Biotechnology
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    • 제3권2호
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    • pp.89-94
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    • 2001
  • In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

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식물육종에 있어서 비환원 ( 2n ) 배우자의 중요성 (Significance of Unreduced ( 2n ) Gametes in Plant Breeding)

  • Rim, Yong-Woo
    • 한국초지조사료학회지
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    • 제17권1호
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    • pp.1-10
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    • 1997
  • Unreduced (2n) gametes are meiotic products (pollen or egg) having a sporophytic (somatic) chromosome number, resulting from abnormalities during either microsporogenesis or megasporogenesis. They occur naturally at a low frequency in many plant species. Unreduced (2n) gametes in plants can be identified for four possible ways as follow i) pollen size and/or shape differences between haploid (n) and diploid (2n) pollen, ii) ploidy analysis (chromosome number) of progeny or meiotic analysis (presence of dyads andlor triads at the microspore stage), iii) progeny performance and fertility and iv) dosage of isozyme and DNA markers. Unreduced (2n) gametes can be an effective breeding tool in synthesizing new cultivars, providing a unique method to maximizing heterozygosity, i.e., transferring a large proportion of the non-additive genetic effects (intra- and inter- locus interactions) h m parent to offspring and can also be used to overcome infertility of interploidy crosses. Sexual polyploidization through 2n gametes has been a major route to the formation of naturally occurring polyploids. The three mechanisms of 2n pollen formation in potato have been discovered as follow: i) parallel spindles (ps) or tripolar spindles (ts), ii) premature cytokinesis (pc-I, pc-2) and iii) synaptic mutants (sy-2, sy-3, sy-4). Genetic analysis indicated that the mechanisms of 2n gamete formation were controlled by single recessive gene in potato, alfalfa, red clover, etc., and by two recessive genes in wheat. The use of 2n gametes which can efficiently transfer germplasm fiom wild relatives to cultivated species, especially fiom diploid to tetraploid could make a contribution to the improvement of germplasm base in breeding programs.

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하전작물 품종 및 재배기술의 1962년 이후 변천 (Changes in Variety and Cultural Practices of Soybean, Sweet Potato and Corn Since 1962 in Korea)

  • 홍은희;박근용
    • 한국작물학회지
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    • 제27권4호
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    • pp.462-469
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    • 1982
  • Since 1962, varietal development and dissemination for summer upland crops have been actively initiated by the Crop Experiment Station, Office of Rural Development, Suweon, Korea. The major breeding objectives of soybeans have been to develop varieties which are early maturing, disease resistant, and adaptable to late planting for after-barley cropping. Development of eleven new soybean varieties including Hwangkeumkong, Jangyeobkong, Danyeobkong, and Kwangkyo has greatly increased the soybean yield throughout country. For com, after development of Hwangok #2, a synthetic, in early 1960's, nine corn hybrids-single crosses, double-crosses, and three-way crosses-such as Jecheon-ok, Hoengseong-ok, Kwangok, Suweon #19, etc., have been disseminated mainly to Kangweon province, a major corn producing area in Korea, and drew up the yield over 4 tons per hectare. The major breeding objectives of sweet potato have been to develop varieties which have high starch content and root yield. Hwangmi, Hongmi, and Shinmi are three sweet potato varieties developed and disseminated by the Crop Experiment Station, Office of Rural Development and are grown most widely in Korea. Most of researches on cultural practices of upland crops have begun on a full scale from early 1960's. In soybeans, for example, no fertilizer but for barley was applied although the effects of phosphate and potassium fertilizers were great on soybeans in after-barley soybean croppings. The effects of heavy application of phosphate and calcium fertilizers on soybeans in newly reclaimed soils were recognized. Recently a mixed fertilizer for soybean (N; 40, P:70, K:60 kg/㏊) was developed and sold for soybean growers. The optimum planting densities of 220, 000 plants/ha in full-season cropping and 330, 000 pts/ha in after-barley cropping of soybeans were known from repeated experiments. For higher yield, a means of cultural practices such as transplanting-pinching, direct planting-pinching, and hilling-up, etc., were developed along with barley-stubble planting with no tillage and integrated herbicide application for labour savings. For sweet potato, cultural practices for planting date, harvesting date, fertilizer, and planting density were fully established. For early marketing, a technique of vinyl-mulching on sweet potato has also fully developed. For com, planting density of 37, 000 pts/ha in early 1960's has been changed to 55, 000 pts/ha for grain production and 67, 000 pts/ha for silage. The amounts of fertilizers have also been changed from 120-120-120kg/ha (N-P-K) in early 1960s to 180-150-150 kg/ha. These increases in number of plants per unit area and fertilizer levels have resulted in greater production for both grain and silage. At the same time, the production techniques of F1 seeds have also improved.

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RAPD에 의한 감자 품종의 구분 (Discrimination of Potato Varieties by Random Amplified Polymorphic DNA Analysis)

  • 서효원;이정윤;조현묵;박영은;오승은
    • 원예과학기술지
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    • 제19권1호
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    • pp.29-33
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    • 2001
  • 감자 DNA의 다형성을 통한 품종 및 육종 계통의 구분이 가능한 특이적 분자 마커를 찾기 위해 URP primer를 이용한 RAPD 분석을 실시하였다. 8 가지의 감자 품종과 5가지의 감자 육종계통을 분석 재료로 이용하였으며, 이들 중 '조풍', '대관70호', '가원'과 '대관 72호'는 '수미', '남작', '남서'와 '대서'를 각각 모본으로 이용되어 육성되었으며, 다양한 표현형질에서 유사성을 가지고 있다. 따라서 생육단계별 표현형질들 만으로는 각각을 구분하기 곤란한 경우가 있다. 벼(Oriza sativa)의 repetitive sequence로부터 고안된 URP primer는 비교적 높은 annealing 온도를 가지며, 20 mer의 비교적 길이가 긴 RAPD용 primer로써 증폭산물의 다형성과 재현성이 높아 다양한 생물종의 구분이 가능한 것으로 보고되고 있다. 국내에서 상품화되어 시판되는 URP primer 들 중에 URP2, URP4, URP8 등이 감자에 적용될 수 있을 것으로 확인하였다. 전기영동된 DNA 밴드들의 유사성을 분석해본 결과 유전적 유사도와는 차이를 나타내고 있었다. 그러나 높은 재현성을 갖는 URP primer들에 의해 증폭된 DNA 밴드의 다형성은 감자의 종내변이를 구분할 수 있는 분자 마커로서 이용할 수 있을 것으로 확인되었다.

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Development of a SCAR Marker Linked to Ph-3 in Solanum ssp.

  • Park, Pue Hee;Chae, Young;Kim, Hyun-Ran;Chung, Kyeong-Ho;Oh, Dae-Geun;Kim, Ki-Taek
    • 한국육종학회지
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    • 제42권2호
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    • pp.139-143
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    • 2010
  • Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.

국내에서 육성한 감자품종의 역병 저항성평가 (Evaluation of Phytophthora Late Blight Resistance of Potato Variety in Korea)

  • 류경열;지형진;심창기;이병모;박종호;최두회;류갑희;박영은
    • 식물병연구
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    • 제12권3호
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    • pp.235-239
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    • 2006
  • Since the cultivation of resistance cultivar is essential for organic agriculture, Phytophthora late blight resistance of 16 advanced potato clones obtained from the potato breeding laboratory at the National Institute of Highland Agriculture was evaluated. Resistance of the clone was examined through artificial inoculation in the laboratory and in the field. Dominant isolates of the pathogen in Gangwon province produced abundant sporangia on leaves of most clones showing susceptibility. The number of sporangia ranged from $10^{7{\sim}8}sporangia/ml$ in the susceptible clones at 7 days after inoculation. However, one clone is resistant in organic farming fields. Disease incidence was 2.3% using the resistant clone. The area under disease progress curve(AUDPC) was 75.5. Contrarily, disease incidence of the susceptible variety was 100% and the AUDPC was 1773.5 during the same cultivation period. The resistance clone named as 'Haryeong' is considered suitable for organic potato cultivation in Gangwon province in Korea.