Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of a SCAR Marker Linked to Ph-3 in Solanum ssp.

  • Park, Pue Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Chae, Young (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Hyun-Ran (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Chung, Kyeong-Ho (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Oh, Dae-Geun (Department of Food Crops, Korea National College of Agriculture and Fisheries) ;
  • Kim, Ki-Taek (The Foundation of Agri. Tech. Commercialization & Transfer)
  • Received : 2010.06.10
  • Published : 2010.06.30
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Abstract

Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.

Keywords

References

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