• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.47 no.5
• /
• pp.85-90
• /
• 2015

To use the African tulip tree (Spathodea campanulata) as raw material for chemical pulping, soda and soda-AQ pulping was investigated. In chemical compositional analysis, lignin contents of African tulip (33.1%) was higher than other hardwood such as Yellow poplar (17.5%), Acacia (27.1%), or Eucalyptus (24.2%). Soda or soda-AQ pulping with African tulip tree resulted in yield at 43.4-44.8% with 29.5-34.5 Kappa number, pulping condition with 20-22% active alkali. Kappa number of African tulip tree pulp was quite higher than other tropical hardwood (Eucalyptus or Accacia) with less yield due to higher lignin content in wood. Fiber length and width of pulp from African tulip tree was similar to Accacia pulp and shorter than eucalyptus pulp.

• Journal of Korean Society of Forest Science
• /
• v.102 no.3
• /
• pp.420-427
• /
• 2013

This study was conducted to analyze growth characteristics and adaptability of poplar clones under the treatment of liquid pig manure. The average of survival rate was 33% better at control than the treatment under age of 5 years. But, tree height and DBH growth were higher at the treatment than control. Populus euramericana 'Eco28' showed the highest survival rate (97.9%) under the treatment. P. euramericana 'Eco28' and P. deltoides hybrid 'Dorskamp' could be selected as superior clones for height and DBH growth under the liquid pig manure treatment. The above-ground biomass production was also investigated when the poplar clones were 5 years old. The aboveground biomass under the liquid pig manure treatment was, on average. 52.6 ton/ha, which was 80% higher than control (29.1 ton/ha). P. euramericana 'Eco28' (73.6 ton/ha) and P. deltoides hybrid 'Dorskamp' (71.1 ton/ha) showed superior biomass production than other clones at the treatment of liquid pig manure. Based on survival, growth and demage traits, the adaptability of poplar clones to liquid pig manure treatment was estimated. P. deltoides hybrid 'Dorskamp' and P. euramericana 'Eco28' showed better adaptability to the treatment. P. nigra ${\times}$ P. maximowiczii '62-10' and P. koreana ${\times}$ P. nigra var. italic 'Suwon' were identified as poor adaptability clones.

• Journal of Korean Society of Forest Science
• /
• v.87 no.1
• /
• pp.50-56
• /
• 1998

Thirty-six $F_3$ hybrid poplar (Populus trichocarpa${\times}$P. deltoides) clones were fumigated with ozone to select for ozone sensitive and resistant clones. Fumigation was applied for 6 to 8 hours each day for approximately 3 months at ozone concentrations of 90 to 115 ppb using by open-top chambers. Height, diameter, number of leaves, total biomass, biomass components, root/shoot ratios, leaf drop and stomatal response were investigated. In summary, ozone generally reduced height, diameter, number of leaves, total biomass, and root/shoot ratios. Ozone stress induced leaf drop and foliar senescence in trees. This study showed very low relationship between total biomass and stomatal conductance. Increased plant resistant to ozone is not always correlated with stomatal behaviour. Probably, characterization of biochemical and other physiological responses to ozone exposure can provide a better understanding of tree response to ozone environment.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.57 no.1
• /
• pp.25-35
• /
• 2015

As livestock manure treatment is becoming a problem, manure application in forest plantation is recommended as an alternative. In this study, to investigate the impact due to liquid manure application in forest plantation, soil, soil water and shallow groundwater quality had been monitored in poplar experimental site where the liquid manure (LM) was applied. Water samples were collected weekly during growing season (April to October) from 2008 to 2011. From the monitoring results, phosphorus concentration in the soil and soil water had no significant difference between LM and control plots. $NO_3$-N concentration of soil water in LM, however, showed higher concentration (13.6 mg/l at 40 cm, 35.1 mg/l at 80 cm) than control plot (1.5 mg/l at 40 cm, 0.5 mg/l at 80 cm). In case of shallow groundwater quality, pH, heavy metal, etc. were satisfied to the national agricultural water quality standard of groundwater and there were no significant difference between upstream and downstream. The $NO_3$-N concentration of shallow groundwater was also not exceeded the national drinking water standard. However, $NO_3$-N concentration in soil water and downstream of shallow groundwater had increased in 2011 when non-composted LM was applied mostly in non-growing season of tree (September). From the results, it is important to control nitrogen source, application time and decomposed or not when LM is applied. In addition, to investigate nitrate source, further long-term monitoring and modelling could be necessary.

• Journal of Korean Society of Forest Science
• /
• v.103 no.2
• /
• pp.189-195
• /
• 2014

Basic leucine zipper (bZIP) protein is a regulatory transcription factor that plays crucial roles in growth, development and stress response of plant. In this study, we isolated a PagbZIP1 gene that belonged to Group SE3 of bZIP from Populus alba ${\times}$ P. glandulosa, and investigated its expressional characteristics. The PagbZIP1 is 844 base pairs long and encodes a putative 144-amino-acid protein with an expected molecular mass of 16.6 kDa. The PagbZIP1 has two conserved domains including the basic and leucine zipper portions. Southern blot analysis revealed that two copies of the gene are presented in the poplar genome. PagbZIP1 was specifically expressed in the root and suspension cells. Moreover, the expression of PagbZIP1 was induced by drought, salt, cold and ABA. Therefore, our results indicated that PagbZIP1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in poplar.

• Journal of Korean Society of Forest Science
• /
• v.98 no.6
• /
• pp.696-702
• /
• 2009

Yellow poplar (Liriodendron tulipifera L.) is an insect-pollinated tree species with large, perfect flower, and its seed sets average only about 10 percent naturally. In its controlled pollination, pollination bags are usually taken to prevent unwanted pollination, but bagging is an expensive and time-consuming process. Therefore, this study was conducted to determine the need of pollination bag by estimating how much unintended pollination would occur when different cross methods were applied. Five different pollination methods were applied as follows: 1) natural open pollination (i.e. insect pollination) as a reference, 2) self-pollination; no removing reproductive organs with bagging, 3) open pollination; emasculated(removing sepal, petal and stamen) without bagging, 4) controlled pollination; emasculated with bagging and 5) controlled pollination; emasculated without bagging. Very low value of full seed rate (0.2%) was observed in method 3, it was suggested that removing stamen and petal restrict the activity of pollen vectors like bee. Difference in the full seed rate between method 4 and method 5 was not significant (27.9% versus 24.0%, respectively). Consequently, controlled pollination without bagging might be an alternative method for extensive breeding and mass production of seeds in yellow poplar.

• Journal of Korean Society of Forest Science
• /
• v.86 no.1
• /
• pp.80-86
• /
• 1997

The objective of this study was to investigate how resistant poplar hybrid makes compensation to ozone stress. Growth, net assimilation rate and initial Rubisco activity were investigated. This study elucidates the physiological mechanisms associated with ozone sensitivity and resistance in 3 selected $F_2$ hybrids, a family originating from a cross between Populus trichocarpa${\times}$P. deltoides. Open-top chambers were used. Ozone concentrations varied from 90 to 115 ppb for 126 days, 6 to 9 hours in a day. This study tested the hypothesis that resistant poplar hybrid maintains the biomass production to ozone exposure via increased net assimilation rate and Rubisco activity. Growth, biomass, net assimilation rate and initial Rubisco activity were generally reduced by ozone treatment. In the tree parts, root under ozone stress was the most sensitive part. Reduced allocation of photosynthates to root growth might be due to increased respiratory demands for maintenance and repair of aboveground tissue damaged by ozone stress. Maintenance or increases remaining leaves in photosynthetic rates and Rubisco activity in resistant clone in response to ozone treatment were the results of biological compensation to ozone stress.

• Korean Journal of Soil Science and Fertilizer
• /
• v.41 no.5
• /
• pp.318-323
• /
• 2008

We studied on the effects of slurry composting and biofiltration liquid fertilizer (SCBLF) on growth of two-year-old poplar clones planted in a reclaimed land mounding soil. The soil on the experimental site had lower concentrations of both exchangeable cations and salinity than before reclamation. However, the content of organic matter was low compared with the most soils. We applied SCBLF to the poplars six times and 5 L in each time for 80 days. Ten clones of six poplar species or hybrids were tested in this study: Populus alba ${\times}$ P. glandulosa(Clivus, 72-30, 72-31, Bongwha1), P. deltoides ${\times}$ P. nigra(Dorskamp), P. deltoides(Lux) ${\times}$ P. deltoides(Harvard)(97-19), P. euramericana(Eco28, I-476), P. nigra ${\times}$ P. maximowiczii(62-2) and P. Koreana ${\times}$ P. nigra var. italica(Suwon). Growth performance varied more among clones than among species. Average height growth of treated plots was 18% greater than control, and clones Clivus, 97-19, Eco28 and Dorskamp were more vigorous than other clones. Diameter at breast height in treated plots was 41% greater than control, and 97-19, Dorskamp, Eco28 and Clivus were the four best clones in this respect. Mean leaf area of treated trees was 26% greater for control trees. Chlorophyll content was similar between treated and controlled trees. Total nitrogen values in leaves were much higher in treated trees 18%. SCBLF treatment on poplars planted in reclaimed land helped tree growth.

• Journal of Plant Biotechnology
• /
• v.48 no.1
• /
• pp.34-43
• /
• 2021

Targeted genome editing using the CRISPR/Cas9 system is a ground-breaking technology that is being widely used to produce plants with useful traits. However, for woody plants, only a few successful attempts have been reported. These successes have used Agrobacterium-mediated transformation, which has been reported to be very efficient at producing genetically modified trees. Nonetheless, there are unresolved problems with plasmid sequences that remain in the plant genome. In this study, we demonstrated a DNA-free genome editing technique in which purified CRISPR/Cas9 ribonucleoproteins (RNPs) are delivered directly to the protoplasts of a hybrid poplar (Populus alba × Populus glandulosa). We designed three single-guide RNAs (sgRNAs) to target the stress-associated protein 1 gene (PagSAP1) in the hybrid poplar. Deep sequencing results showed that pre-assembled RNPs had a more efficient target mutagenesis insertion and deletion (indel) frequency than did non-assembled RNPs. Moreover, the RNP of sgRNA3 had a significantly higher editing efficacy than those of sgRNA1 and sgRNA2. Our results suggest that the CRISPR/Cas9 ribonucleoprotein-mediated transfection approach is useful for the production of transgene-free genome-edited tree plants.

• Journal of Mushroom
• /
• v.14 no.3
• /
• pp.105-110
• /
• 2016

This study was carried out to develop techniques for the log cultivation of Pleurotus pulmonarius. Soil landfill cultivation of the in plastic container boxes containing yield per log than but there was no difference in the yield from both spawn Hyangsan" variety. In the case of soil landfill cultivation in a shaded vinyl house, an Ailanthus tree gave a higher yield than that using poplar tree, and the yield of the Hosan" variety was higher than that of the Hyangsan" variety. With regard to proper tree species selection, willow and cherry trees were good for both the Hosan" and "Hyangsan" variet.