Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Isolation and Characterization of a Basic Leucine Zipper Gene in Poplar (Populus alba × P. glandulosa)

현사시나무에서 Basic Leucine Zipper 유전자의 분리와 특성 구명

  • Yoon, Seo-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Lee, Hyoshin (Division of Forest Tree Improvement, Korea Forest Research Institute) ;
  • Bae, Eun-Kyung (Division of Forest Tree Improvement, Korea Forest Research Institute) ;
  • Choi, Young-Im (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Kim, Joon-Hyeok (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Noh, Seol Ah (Division of Forest Biotechnology, Korea Forest Research Institute)
  • 윤서경 (국립산림과학원 산림생명공학과) ;
  • 이효신 (국립산림과학원 임목육종과) ;
  • 배은경 (국립산림과학원 임목육종과) ;
  • 최영임 (국립산림과학원 산림생명공학과) ;
  • 김준혁 (국립산림과학원 산림생명공학과) ;
  • 노설아 (국립산림과학원 산림생명공학과)
  • Received : 2014.01.09
  • Accepted : 2014.05.07
  • Published : 2014.06.30
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Abstract

Basic leucine zipper (bZIP) protein is a regulatory transcription factor that plays crucial roles in growth, development and stress response of plant. In this study, we isolated a PagbZIP1 gene that belonged to Group SE3 of bZIP from Populus alba ${\times}$ P. glandulosa, and investigated its expressional characteristics. The PagbZIP1 is 844 base pairs long and encodes a putative 144-amino-acid protein with an expected molecular mass of 16.6 kDa. The PagbZIP1 has two conserved domains including the basic and leucine zipper portions. Southern blot analysis revealed that two copies of the gene are presented in the poplar genome. PagbZIP1 was specifically expressed in the root and suspension cells. Moreover, the expression of PagbZIP1 was induced by drought, salt, cold and ABA. Therefore, our results indicated that PagbZIP1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in poplar.

Basic leucine zipper(bZIP) 단백질은 식물의 성장과 발달 그리고 스트레스 반응을 조절하는 전사인자이다. 본 연구에서는 현사시나무(Populus alba ${\times}$ P. glandulosa)에서 SE3 그룹에 속하는 bZIP인 PagbZIP1 유전자를 분리하여 구조와 발현 특성 등을 조사하였다. 현사시나무의 PagbZIP1 유전자는 844개의 염기쌍으로 이루어져 있으며, 144개의 아미노산으로 구성되는 예상 분자량 16.6 kDa의 단백질을 암호화한다. PagbZIP1은 보존영역인 basic domain과 leucine zipper domain을 가지고 있으며, 현사시나무의 염색체에 2 copy가 존재하는 것으로 나타났다. PagbZIP1은 현사시나무의 뿌리와 배양세포에서 특이적으로 발현하며, 배양세포의 생장주기에서는 정지기에 높게 발현하였다. 또한 건조와 염 그리고 저온 스트레스 뿐 아니라 식물호르몬인 ABA 처리에 의해서도 발현이 유도되어, ABA를 경유한 신호전달경로를 따라 유전자 발현을 조절하는 것으로 판단되었다. 본 연구결과는 PagbZIP1 유전자의 도입과 발현조절을 통한 바이오매스 증진 및 스트레스 내성 나무의 개발에 도움을 줄 것으로 생각된다.

Keywords

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