• Korean Journal of Environmental Agriculture
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• v.27 no.2
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• pp.185-190
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• 2008

In order to obtain the basic information for agricultural utilization of Germanium(Ge), the growth characteristics and the germanium uptake by water celery were investigated at different concentration of germanium in soil. This experiment was carried out in the Wagner pot(1 $5,000^{-1}a$). Germanium concentrations in soil for water celery cultivation were maintained at 0.26, 25.0, 62.5, and 125.0 mg $kg^{-1}$, respectively. The treatment of over Ge 25.0 mg $kg^{-1}$ in the soil led to germanium phytotoxicity such as reduction of plant height and fresh weight. The contents of germanium in water celery were increased with the increase of germanium concentration in the soil. When water celery was cultivated from soil maintained with Ge 25.0 and 62.5 mg $kg^{-1}$, its germanium contents in plant were 89.9 and 371.6 mg $kg^{-1}$, respectively. Then, the efficiency of germanium uptake of water celery in Ge 25.0 and 62.5 mg $kg^{-1}$ maintained plots was 1.7 and 2.4%, respectively. When water celery was cultivated from soil maintained with Ge 25.0, 62.5 and 125.0 mg $kg^{-1}$, its content of amino acid was found to be 89.8, 198.4, and 318.2 mg $g^{-1}$, respectively. To investigate the effect of N fertilizer application in uptake of germanium by water celery, these were treated with nontreatment, 1.0, 1.5 and 2.0 times of N application based on soil testing for cultivation of water celery. However, the amount of the N fertilizer application did not affect the contents of germanium in the water celery. When water celery was cultivated from soil maintained with two kinds of inorganic and organic germanium 50 mg $kg^{-1}$, respectively, the content of germanium were 24.2 mg $kg^{-1}$ in the Ge-132 treatment and 11.8 mg $kg^{-1}$ in the $GeO_2$ treatment.

• The Journal of the Korea Contents Association
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• v.8 no.8
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• pp.285-292
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• 2008

This study was initiated to evaluate the Phosphorus(P) leaching potential in the putting green soils and P uptake by the turfgrass in the golf course using the P fertilizers. The turfgrass, Floradwarf bermudagrass(Cynodon dactylon L. PERS,) was planted and grown in the mixture of sand and peat moss in this lysimeter study. Five representative P fertilizers, such as, ammonium polyphosphate (APP), monopotassium phosphate (MKP), MAP(monoammonium phosphate), 0-20-20(liquid), and concentrated superphosphate(CSP, solid) were used in this study. Based on the total P quantity of leachate collected during the whole 12 weeks, MKP and APP are the first group of P fertilizers contributing to the leaching of P, then MAP and 0-20-20 are the second group of P fertilizers causing the P leaching. Finally, CSP is the third group of P fertilizer resulting in the P leaching. However, most of P applied and collected in the lysimeter were leached during the first period of two and four weeks, compared to that of P leached during the second period of six, eight, ten, and twelve weeks. Applications of MAP, APP and CSP, MKP and 0-20-20 in order produced the largest amount of total dry matter. However, APP, MKP and MAP, CSP and 0-20-20 in order showed the largest amount of P uptake. Therefore, based on the data of P leaching, dry matter production, and plant P uptake, it appears that CSP, 0-20-20, and MAP are the environmentally sound fertilizers recommended in the turfgrass putting green soil of golf course.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.293-303
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• 2017

To elucidate the physiological responses of rice plants to the essential mineral silicon (Si), we assessed the effects of treatments with Si, nitrogen ($NH_4NO_3$; ammonium nitrate), and calcium ($CaCl_2$; calcium chloride), independently or in combination on mineral uptake rates and levels of the hormones abscisic acid (ABA), gibberellin ($GA_1$) and jasmonic acid (JA). We found that nitrogen and calcium uptake was inhibited by Si application. However, solo application of nitrogen or calcium did not affect Si uptake. Compared to the untreated plants, the application of Si, $NH_4NO_3$ or $CaCl_2$ increased the endogenous hormone levels in treated plants. In particular, the concentrations of $GA_1$ and JA increased significantly after the application of Si or $NH_4NO_3$. The level of $GA_1$ observed after a treatment (solo or combine) with Si, and $NH_4NO_3$ was higher than that of the control. By contrast, independent application of $CaCl_2$ or a combined treatment with Si and $CaCl_2$ did not alter $GA_1$ levels. The highest level of $GA_1$ was present in plants given a combination treatment of Si and $NH_4NO_3$. This effect was observed at all time points (6 h, 12 h and 24 h). Endogenous JA contents were higher in all treatments than the control. In particular, a combination treatment with Si and $NH_4NO_3$ significantly increased the JA levels in plants compared to other treatments at all time points. A small increase in JA levels was observed after 6 h in plants given the $CaCl_2$ treatment. However, JA levels did not differ between plants given a $CaCl_2$ treatment and controls after 12 h or 24 h of exposure. We conclude that treatment with $CaCl_2$ alone does not affect endogenous JA levels in the short term. Endogenous ABA contents did not show any differences among the various treatments.

• Korean Journal of Medicinal Crop Science
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• v.17 no.5
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• pp.363-368
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• 2009

To fulfill the increasing demand for a high quality of flower, we investigated the effects of nitrogen application on plant growth, yield and bioactive compounds of Chrysanthemum indicum L.. C. indicum L. was cultivated in a pot scale, and nitrogen applied with the level of 0 (N0), 50 (N50), 100 (N100), 150 (N150), 200 (N200) and $300\;(N300)\;kg\;ha^{-1}$ to suggest optimum rate of nitrogen fertilization. Phosphate and potassium applied the same amount of $80-80\;kg\;ha^{-1}$ ($P_2O_5-K_2O$) in all treatments. Growth characteristics and yields of C. indicum L. were significantly affected by nitrogen application. Maximum yield achieved in 265 and $295\;kg\;ha^{-1}$ N treatment on the whole plant and the flower parts, respectively. The nitrogen content and uptake of whole plant significantly increased by the increase of nitrogen application. Five major components of essential oil, $\alpha$-pinene, 1,8-cineol, chrysanthenone, germacrene-D, and $\alpha$-curcumene in flowerheads of C. indicum L. occupied approximately 40% of peak area, germacrene-D decreased by the increase of nitrogen application among them. However, cumambrin A contents in the flower parts of C. indicum L. were affected negatively by the increase of nitrogen application, but total yields of cumambrin A in flower part significantly increased. Conclusively, nitrogen fertilization could increase the yield of flowerheads. The optimum application level of nitrogen fertilizer might be on the range of $265-295\;kg\;ha^{-1}$ in a mountainous soil.

• Journal of Plant Biology
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• v.34 no.2
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• pp.129-136
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• 1991

Two cell types, tip cells and hyphal cells, were found at the front of Cuscuta australis endophyte growing into the stem parenchyma of the host plant, Trifolium repens. Each tip cell developed into an elongate, filamentous hypha. The cells of both types possessed a dense cytoplasm including abundant organelles and enlarged nuclei with the deeply lobed envelope. The unevenly thick walls were observed in certain tip cells. The wall penetrated through the middle lamellae of the host cells and engulfed the debris of broken host cells. Some front cells had the plasmalemma-wall invaginations, which increased the surface area and would facilitate material uptake from the host No plasmodesmata between the host and parasite cells were found; instead, an apoplastic continuity was established by fused cell walls at the interface of the two partners. The apoplast was thought to be the main route for water and nutrients transport.nsport.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.171-178
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• 2013

BACKGROUND: Consensus of heavy metal phytoavailability in soils needs to be introduced for soil management protocols in relation to safer food production in the contaminated agricultural soils. For this, setting up the method for evaluation of metal phytoavailability in soil is an essential prerequisite. METHODS AND RESULTS: The current study was carried to select a proper single extraction method for determination of phytoavailable metal concentration in soil. Two extraction methods were examined including 1 M $NH_4NO_3$ extraction and 0.01 M $Ca(NO_3)_2$ extraction methods using 142 soil samples collected from the agricultural soils nearby abandoned mining area in Korea. Corelation analysis was conducted between phytoavailable metal concentrations and soil properties potentially influencing on the metal phytoavailability. Both methods showed similar significance (p<0.001) in correlation with soil properties such as soil pH. However, higher correlation coefficients between phytoavailable metal concentrations and soil properties were observed when used $Ca(NO_3)_2$ extraction rather than using $NH_4NO_3$ extraction. CONCLUSION(S): It appeared that 0.01 M $Ca(NO_3)_2$ extraction was better option for determination of phytoavailable metals in soils and further study to test the efficiency of this method is required in combination with plant uptake.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.409-413
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• 2015

To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.1
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• pp.7-10
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• 1987

This study was conducted to evaluate the effect of a newly developed paste fertilizer on rice growth. It was applied to two vars. of Taeback and Cheonma byeos as a basal treatment. It needs a unique applicator attached to transplant machine for rice plant and was injected about 3cm depth of paddy soils. The polished rice yields were obtained competitively with a 20 percent reduction of N applied with top dressing. Plant development treated paste fertilizer was good compared to control at the early growing stage, however, not different at the late growing stage. Nitrogen uptake in plant was high at the early growing stage in paste fertilizer treatment but there were no significant difference at the late growing stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.248-251
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• 2003

To find ideal leaf types for soybean breeding program, we examined the relationships among leaf anatomical and morphological characteristics and $\textrm{CO}_2$ assimilation on the different leaflet shape of soybean (Glycine max). In anatomical characters of leaf, palisade and spongy cells were thicker in both small seed cultivars with narrow leaflet and large seed cultivars with wide leaflet than others. $\textrm{CO}_2$ uptake per plant and leaf thickness were significantly associated with seed yield per plant, showing difference among the soybean cultivars. Although the leaf area was lower for narrow leaflet cultivars, which had a significantly higher photosynthetic rate per plant comparable to the wide leaflet cultivars.

• Applied Biological Chemistry
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• v.40 no.4
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• pp.352-356
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• 1997

To induce the phytoalexins in plant cell culture systems, we surveyed the antimicrobial activity following the feeding of five naphthoquinones in cell cultures of petunia. Among naphthoquinones treated, 2,5,7-trihydroxy-3-(5'-hydroxyhexyl)-1,4-naphthoquinone (3-OH NQ ) was most efficiently absorbed into the cells within 48 hr. The crude extracts of cells treated with 3-OH NQ showed a strong inhibition activity on spore germination of Aspergillus candidus $(MIC:\;32\;{\mu}g/ml)$, whereas the untreated cells showed no activity. The two active compounds, 4,2',4',${\beta}$-tetrahydroxychalcone and 4',7-dihydroxyflavone, were isolated from petunia cells treated with 3-OH NQ. The major phytoalexin, 4,2',4',${\beta}$-tetrahydroxychalcone, inhibited strongly the spore germination of A. candidus $(MIC:\;16\;{\mu}g/ml)$.