Applied Biological Chemistry
- Volume 40 Issue 4
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- Pages.352-356
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- 1997
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Induction of Phytoalexins by Uptake of Naphthoquinones in Cell Cultures of Petunia
Naphthoquinone류 화합물 흡수에 의한 페튜니아 배양세포내의 Phytoalexin 유도
- Kim, Myong-Jo (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Kwak, Sang-Soo (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology)
- Published : 1997.08.30
Abstract
To induce the phytoalexins in plant cell culture systems, we surveyed the antimicrobial activity following the feeding of five naphthoquinones in cell cultures of petunia. Among naphthoquinones treated, 2,5,7-trihydroxy-3-(5'-hydroxyhexyl)-1,4-naphthoquinone (3-OH NQ ) was most efficiently absorbed into the cells within 48 hr. The crude extracts of cells treated with 3-OH NQ showed a strong inhibition activity on spore germination of Aspergillus candidus
식물배양세포계에서 유용 phytoalexin의 생산여부를 검토하기 위하여 페튜니아 배양세포주에 5종의 naphthoquinone류 화합물을 첨가하였을 때 유도되는 항미생물활성을 조사하였다. 처리한 naphthoquinone류 화합물 중 토양 방선균으로부터 페튜니아 세포생장억제물질로 분리한 2,5,7-trihydroxy-3-(5'-hydroxyhexyl)-1,4-naphthoquinone (3-OH NQ)이 48시간 이내에 90% 이상 효율적으로 배양세포내로 흡수되었다. 3-OH NQ를 처리한 페튜니아 캘러스 추출물은 Aspergillus candidus의 포자발아를 강하게 억제하였다