• Research in Plant Disease
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• v.15 no.2
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• pp.120-126
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• 2009

In the course of study on the roles of phytotoxins in the pathogenicity of Botrytis cinerea, we isolated two novel phytotoxins. They were identified as 3-O-acetyl botcinol and 3-O-acetyl botcinolide. In this study, we investigated correlation between the two phytotoxins and the pathogenicity of B. cinerea. In liquid cultures, the two phytotoxins were not produced by three low pathogenic isolates out of 25 B. cinerea isolates. Among strong or moderate pathogenic isolates, some produced the two phytotoxins, but the others did not. On the other hand, the ethyl acetate extracts of fermentation broths of 10 out of 25 isolates showed phytotoxic activity against various plants tested in a whole plant assay. The phytotoxins were detected in all of the 10 phytotoxic ethyl acetate extracts. In planta, the two phytotoxins were detected in all of the plant tissues infected with strong pathogenic isolates. However, there was no correlation between the ability of B. cinerea isolates to produce the two phytotoxins and their pathogenicities. The two phytotoxins began to detect in tomato plant tissues infected with B. cinerea 2-16 at 3 days after inoculation, increased gradually till 4 days after inoculation, and then decreased. The above results suggest that 3-O-acetyl botcinol and 3-O-acetyl botcinolide are one of pathogenicity factors for B. cinerea, but not a primary determinant of its pathogenicity.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.619-627
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• 2001

n order to increase the virus safety of a human intravenous immunoglobulin (IVIg) that was manufactured by a successive process of cold ethanol fractionation, polyethylene glycol precipitation, and pasteurization ($60^{\circ}C$ heat treatment for 10h), a low pH incubation process (pH 3.9 at $25{\circ}C$ for 14 days) was employed as the final step. The efficacy and mechanism of the fraction III cold ethanol fractionation, pasteurization, and low pH treatment steps in the removal and/or inactivation of blood-borne viruses were closely examined. A variety of experimental model viruses for human pathogenic viruses, including the Bovine herpes virus (BHV), Bovine viral diarrhoea virus (BVDV), Murine encephalomyocarditis virus (EMCV), and Porcine parvovirus (PPV), were selected for this study. The mechanism of reduction for the enveloped viruses (BHV and BVDV) during fraction III fractionation was both inactivation and partitioning, however, it was partitioning in the case of the nonenveloped viruses (EMCV and PPV). The log reduction factors achieved during fraction III fractionation were ${\geqq}$6.7 for BHV, ${\geqq}4.7$ for BVDV, 4.5 for EMCV, and 4.4 for PPV. Pasteurization was found to be a robust and effective step in inactivating all the viruses tested. The log reduction factors achieved during the pasteurization process were ${\geqq}7.5$ for BHV, ${\geqq}4.8$ for BVDV, 3.0 for EMCV, and 3.3 for PPV. A low pH incubation was very effective in inactivating the enveloped viruses as well as EMCV. The log reduction factors achieved during low pH incubation were ${\geqq}7.4$ for BHV, ${\geqq}3.9$ for BVDV, 5.2 for EMCV, and 2.0 for PPV. These results indicate that the low pH treatment successfully improved the viral safety of the final products.

• Journal of Korean Medical classics
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• v.24 no.4
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• pp.117-142
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• 2011

This paper is about the emotional factors in Chiljeongsang(七情傷). To specify the collective term into specific categories, an examination of the meaning of emotion of the East and West was primarily undertaken. The importance of emotion lies in the fact that it provides a link between the individual and the outside world. Emotion was perceived as reflections of the human nature and mental activity by philosophers and psychiatrists throughout history. In the views of psychologists, the focus was on not emotion itself, but rather the emotional experience in human, and its psychological and physiological mechanism. In Traditional Medicine, problematic emotion is called Chiljeong(七情). The term Chiljeongsang(七情傷), which is a compound word of 'Chiljeong(七情)' and 'Sang(傷)' meaning 'damage', is used in referring to illness related to unhealthy emotions. It is used to describe illness caused by Chiljeong(七情) and mental disorders that result in emotional symptoms. It also refers to illness where Chiljeong(七情) plays a role in creating a more direct cause of illness in the larger pathogenic process. In the contents of Chiljeongsang(七情傷) in Traditional Medicine, emotion as causation of Gi(氣) disorder could be found the most, while explanation on the psychological process related to the creation of Chiljeong(七情) was insufficient. This tendency is related to the philosophical basis of Traditional Medicine. Out of the two patterns suggested, the mind-based Chiljeongsang(七情傷) pattern includes the psychological aspects of damage, in which key focus is on the cause that arouses problematic emotion, its process and the meaning this emotion entails. To explain this, concepts from psychiatry, especially Jung's theory on neurosis was adopted. In treating a Chiljeongsang(七情傷) patient, mere knowledge of the physiological changes of the body in terms of Gi(氣) is insufficient. This is because mental factors play a large role in Chiljeongsang(七情傷), and the more a condition is 'mental', the more complex it becomes and the more it influences life as whole. Therefore, the doctor must approach the patient with not only medical knowledge, but also overall knowledge on all aspects of human life.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.189-199
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• 2019

The purpose of this study is to derive spatial area(preventive zone) where the movement of livestock vehicles occurs frequently. For this purpose, this study used 6 periods facility entrance data provided by KAHIS. This data was converted into vehicle movement data between livestock facilities and aggregated into administrative district units. The R-mode factor analysis was performed on the constructed OD data, and the region extracted by the same factor was judged as one region. The results of the analysis are summarized as follows. First, the factor analysis of 6 periods data showed 16 ~ 18 factors, and the derived factors explained 63 ~ 68% of the total variance. Second, based on the factors that were derived, Jeonam coastal area, Jeonnam area, Jeonbuk area, Chungnam coastal area, Gyeongnam area, northern Gyeongbuk area, Yeongnam costal area were found to be stable, with little change over time. On the other hand, Chungbuk area, Gangwon area, Seoul metropolitan area are relatively volatile areas. Third, 13 areas were derived by combining data from six periods.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.3
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• pp.286-293
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• 2019

Hypertension (HTN) is one of the major chronic diseases, and HTN is defined as being in a state of continuous high blood pressure. Left ventricular hypertrophy (LVH) is a condition in which the mass of the left ventricle has increased, and HTN is a leading cause of LVH. HTN and LVH are known to be caused by the interaction of environmental factors and genetic factors. It has been reported that the polymorphisms of SLC8A1, among the genetic factors that affect high blood pressure, are related to salt sensitivity hypertension. In this study, the genetic polymorphisms of SLC8A1 were chosen based on the Korean Genome and Epidemiology data. Logistic regression analysis was then performed for HTN and LVH. Linear regression analysis was also performed for systolic blood pressure (SBP) and diastolic blood pressure (DBP). As a result, 5 SNPs showed statistically significant associations (P<0.05) with HTN, and 10 SNPs showed statistically significant associations with LVH. rs1002671 and rs9789739 showed significant correlation at the same time with HTN and LVH. These results suggest that the polymorphisms of the SLC8A1 gene are linked to the development of HTN and LVH in Koreans. We expect these results to help us understand the pathogenic mechanisms for HTN and LVH.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.2
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• pp.127-132
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• 2015

In order to grasp the clear concepts and variables to treat Shanghan diseases which has complex meaning by each medical literature, several concepts and analytic method of structuralism from early to late stage encompassing Saussure and Derrida were used. Main concepts are langue and parole, signifiant and signifie, syntagme and paradigme, denotation and connotation, synchronie and diachronie, identity of structure and differance etc. and methods are substituting these concepts to historical Shanghan theories from Zhongjing to Ming-Qing dynasty and comparisons of synchronie about their era. Essential qualities of Shanghan diseases are pathologic phenomena under the order of unification of nature and man formed through concrescence between individual human body and geo-climatico-socio-cultural environmental conditions, neither injuries by cold pathogen nor five types of exopathogenic febrile diseases. The former environmental elements can be inferred from general traits of desires and public pathological aspects of social members, and the latter personal factors from corresponding features to those pathogenic variables. In addition, the concepts of Shanghan disease are added successively up to now via Jin-Yuan's four great masters and Wenbing masters, and thus the new concepts of denotation became another connotation obtaining new signifiant. In this way, for the treatment of Shanghan diseases, new theories should be made for ranging prescription over the wenbing field; reflecting not only climatical variables but also each patient's physio-pathological features and sociocultural variables. Thereby an appropriate and reasonable therapeutical systems can be designed and can guide research direction hereafter.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.11-22
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• 2018

Type VI secretion system (T6SS) has been discovered in a variety of gram-negative bacteria as a versatile weapon to stimulate the killing of eukaryotic cells or prokaryotic competitors. Type VI secretion effectors (T6SEs) are well known as key virulence factors for important pathogenic bacteria. In many Burkholderia species, T6SS has evolved as the most complicated secretion pathway with distinguished types to translocate diverse T6SEs, suggesting their essential roles in this genus. Here we attempted to detect and characterize T6SSs and potential T6SEs in target genomes of plant-associated and environmental Burkholderia species based on computational analyses. In total, 66 potential functional T6SS clusters were found in 30 target Burkholderia bacterial genomes, of which 33% possess three or four clusters. The core proteins in each cluster were specified and phylogenetic trees of three components (i.e., TssC, TssD, TssL) were constructed to elucidate the relationship among the identified T6SS clusters. Next, we identified 322 potential T6SEs in the target genomes based on homology searches and explored the important domains conserved in effector candidates. In addition, using the screening approach based on the profile hidden Markov model (pHMM) of T6SEs that possess markers for type VI effectors (MIX motif) (MIX T6SEs), 57 revealed proteins that were not included in training datasets were recognized as novel MIX T6SE candidates from the Burkholderia species. This approach could be useful to identify potential T6SEs from other bacterial genomes.

• Journal of Environmental Health Sciences
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• v.42 no.4
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• pp.255-265
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• 2016

Objectives: Mobile phones have become one of the most essential accessories in daily life. However, they may act as reservoir of infectious pathogens if they are used without hygienic practices in their handling. Therefore, this study aimed to isolate food-borne pathogens from mobile phones and investigate the characteristics of toxin genes and antibiotic susceptibility patterns. Methods: A total of 146 mobile phones were collected from 83 middle- and 63 high-school students in Busan. The surfaces of the mobile phones were aseptically swabbed. Results: Among the food-borne pathogens, Staphylococcus aureus, Bacillus cereus and Escherichia coli were detected in 26 (17.8%), 20 (13.7%) and four (2.7%) samples, respectively. There were no statistically significant differences according to school level, gender or phone type. None of four E. coli strains had pathogenic toxic genes. All of the B. cereus strains carried at least three different toxin genes among the nine enterotoxin and emetic toxin genes. Three out of 20 B. cereus strains (15%) possessed emetic toxin genes, which are rarely detected in food-poisoning cases in Korea. Among the 26 strains of S. aureus, the detection rate of staphylococcal enterotoxin genes, toxic shock syndrome toxin (tsst) and factors essential for methicillin resistance (femA) were 84.6%, 7.7% and 100%, respectively. In the antibiotic susceptibility test, there was no methicillin-resistant S. aureus (MRSA) or vancomycin-resistant S. aureus (VRSA). Conclusion: The results show that students' mobile phones in Busan were contaminated by food-borne pathogens which carried various toxic genes. Therefore, regular phone disinfection and hand hygiene is important in order to reduce cross-contamination.

• Journal of Sasang Constitutional Medicine
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• v.9 no.2
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• pp.95-145
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• 1997

This study was performed to reveal some contrasting aspects between Sasang Constitutional Medicine(四象醫學) and Traditional Oriental Medicine(傳統 韓醫學). This aims ultimately for the unified standardization of Korea-Oriental Medicine. Suggesting the clear contrasting aspects of thse two medical system, this study might give several basic informations for ultimate achievement. As a result, some contrasting aspects were revealed as follows. 1. The interpretation of the man's status is contrasted. In Sasang Constitutional Medicine, man is interpreted as the member of the Society(天下) while in Traditional Oriental Medicine as the part of the Nature(天地). 2. The man's modeling is contrasted. In Sasang Constitutional Medicine, man is in the process of becoming as differentiating itself by the inner emotional producing. While in Traditional Oriental Medicine, man is generalized as one model according to the Nature, one great universe(天地). 3. The pathogenesis is contrasted. In Sasang Constitutional Medicine, emotional inclination is the main factor provoking the primary pathogenesis. While in Traditional Oriental Medicine the external isolated pathogenic factors such as Cold, Heat, Warm etc. are significant for the pathogenesis. 4. The evaluation of the disease is contrasted. In Sasang Constitutional Medicine all the diseases are evaluated according to the inherent condition of patient himself. But in Traditional Oriental Medicine the evaluation of disease isolated from the inheret condition of patient himself is generalized in its own system. 5. The prime method of healing, controlling, and maintaining health of the patient is contrasted. For these, the patient's spontaneous effort for adjusting his emotional inclination is important in Sasang Constitutional Medicine. While in Traditional Oriental Medicine, the external adjustment dependent on the herbal medication is the main method for healing, controlling, and maintaining health of the patient.

• Journal of Korean Medical classics
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• v.30 no.1
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• pp.111-133
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• 2017

Objectives : Dryness pathogen, which is one of six pathogenic factors, causes dryness diseases. Currently, the theory on dryness disease is composed of external dryness and internal dryness. External dryness, in turn, is composed of cool dryness and warm dryness. However, these categorizations and their symptoms bear ambiguity for many reasons. Therefore, this paper aims to review various texts in order to study the special features of dryness pathogen and dryness disease. Methods : Texts that deal with dryness pathogen and dryness disease were studied. Most texts are comprised of dissertations and historical medical texts, therefore, CNKI and The Sikuquanshu's databases, and Traditional Chinese Medical(TCM) book webdatabases were utilized. Materials are listed in chronological order, and their main points regarding dryness pathogen and dryness disease are compared. Results & Conclusions : It is difficult to accept the assertion that dryness pathogen does not lead to external dryness. Dryness does not have the elements of chill and fever in itself. Dryness's elements of chill and fever are determined in the ways they combine with each individual element. Moreover, the symptoms of chill and fever on dryness disease are subject to the host's body type. External dryness and internal dryness cannot be discussed within an identical premise. Whereas the dryness in external dryness signifies the cause of a disease, the dryness in internal dryness is the consequence of a disease. In other words, internal dryness revolves around cause of disease and external dryness revolves around the mechanic of disease. It's difficult to determine whether these diseases are caused by dryness or wetness in Autumn. There is an understanding which integrates these together through the Yunqi theory, but it is imperfect.