• Korean Journal Plant Pathology
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• v.10 no.3
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• pp.151-156
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• 1994

Pseudomonas fluorescens produces the antibiotic, 2,4-diacetylphloroglucinol (Phl), which promotes plant growth by inhibiting bacteria and fungi. Cosmids (genomic library) were mobilized into Phl-nonproducing mutants through the triparental matings with pRK2013 as the helper plasmid at the frequency of 8.37$\times$10-4. Complemented mutants that showed antibiotic activity were selected among about 2,000 transconjugants. The complemented mutants were confirmed by acquired drug resistances (kanamycin and tetracycline). The antibiotic substances of wild type and complemented mutants showed the most excellent anti-bacterial activity. Inhibitory effects of complemented P. fluorescens against phytopathogenic fungi were equal to the parental strain. Complemented mutant and wild type of P. fluorescens were causal microbes of fungal morphological abnormalities. Complemented mutants in potato dextrose agar supplemented with bromothymol blue also showed restoration of glucose utilization as wild type. Plasmids of complemented mutants were isolated from transconjugant sand transformed into competent cells of E. coli DH5$\alpha$. The plamid DNA was reisolated from transformed E. coli DH5$\alpha$.

• Child Studies in Asia-Pacific Contexts
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• v.4 no.2
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• pp.95-108
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• 2014

The purpose of this study was to examine group differences in paternal behaviors (i.e., paternal connection, paternal punitiveness, and paternal knowledge) within and across three academic achievement levels-low, moderate, and high, and to explore the effects of paternal behaviors on the academic motivation of Chinese adolescents within these three achievement groups. Analyses of variance (ANOVAs) indicated that adolescents with low achievement perceived their fathers as more punitive than teens with moderate and high academic achievement. Regression analyses also revealed that paternal punitiveness (negative) and paternal knowledge (positive) were significant predictors of academic motivation for teens with low levels of academic achievement; while paternal punitiveness was a significant negative predictor of academic motivation among adolescents with moderate achievement. In contrast, for adolescents with high achievement, paternal connection was a positive significant predictor of academic motivation. The present findings provide some evidence that the impact of parental behaviors on teen's motivation varies across adolescent academic achievement levels, which may prove useful for professionals working with fathers to help target the most effective parenting behaviors to foster academic motivation.

• Journal of Preventive Medicine and Public Health
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• v.48 no.6
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• pp.319-329
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• 2015

Objectives: Parental socioeconomic status (SES) exerts a substantial influence on children's health. The purpose of this study was to examine factors determining children's private health insurance (PHI) enrolment and children's healthcare utilization according to PHI coverage. Methods: Korea Health Panel data from 2011 (n=3085) was used to explore the factors determining PHI enrolment in children younger than 15 years of age. A logit model contained health status and SES variables for both children and parents. A fixed effects model identified factors influencing healthcare utilization in children aged 10 years or younger, using 2008 to 2011 panel data (n=9084). Results: The factors determining children's PHI enrolment included children's age and sex and parents' educational status, employment status, and household income quintile. PHI exerted a significant effect on outpatient cost, inpatient cost, and number of admissions. Number of outpatient visits and total length of stay were not affected by PHI status. The interaction between PHI and age group increased outpatient cost significantly. Conclusions: Children's PHI enrolment was influenced by parents' SES, while healthcare utilization was affected by health and disability status. Therefore, the results of this study suggest disparities in healthcare utilization according to PHI enrollment.

• Biomolecules & Therapeutics
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• v.6 no.4
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• pp.345-350
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• 1998

The adverse effects of ethanol on cell proliferation have been described for a variety of tissues and cells. In the present study, we investigated whether chronic ethanol intoxication impairs the cell proliferation and DNA synthesis induced by oncogenic $H-ras^{V12}$ - and $v-K-ras^{V12}$-transformed cells. Ethanol treatment inhibited the cell proliferation and the DNA synthesis of control parental fibroblasts in a time- and dose-dependent manner. In contrast, ethanol did not suppress the proliferation of either oncogenic $H-ras^{V12}$ - or $v-K-ras^{V12}$ -transformed fibroblasts. Microinjection of oncogenic $H-Ras^{V12}$ protein induces DNA synthesis and ethanol treatment did not interfere with the DNA synthesis. The antiproliferative toxicity of ethanol was rescued by antioxidants, such as N-acetylcysteine and 4-methlpyrazole. These results indicate that the antiproliferative action site of ethanol toxicity lies upstream or is independent of Ras and ethanol exerts its toxicity through a free radical formation.

• Korean Journal of Child Studies
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• v.31 no.3
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• pp.255-272
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• 2010

This study explored pathways from mothers' beliefs to children's subjective well-being through children's private after-school activities and stress levels. A sample of 230 6th grade elementary school students (125 boys and 105 girls) in Seoul completed questionnaires on children's stress and subjective well-being. Their mothers responded to questionnaires on mothers' beliefs and children's private after-school activities. Data were analyzed by means of Pearson's correlation coefficients and multiple regression analyses. Our results demonstrated that mothers' beliefs indirectly influenced children's subjective well-being through both children's private after-school activities and stress levels. Neither children's private after-school activities nor children's stress mediated between mothers' beliefs and children's subjective well-being. Mothers' beliefs also had a direct effect on children's subjective well-being. Significantly, both mothers' beliefs and children's stress played crucial roles in improving children's subjective well-being.

• Korean Journal of Child Studies
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• v.37 no.3
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• pp.27-38
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• 2016

Objective: This study examined the influence of maternal attachment on adjustment in adolescence and investigated whether paternal attachment had any moderating effects on the relationship between maternal attachment and adjustment in adolescence. Methods: The participants of this study were 300 second graders from two middle schools in Seoul. We measured paternal and maternal attachment as perceived by the participants, adjustment in adolescence through school life adjustment, depression, and relational aggression. The study applied correlation analysis and hierarchical regression analysis suggested by Baron and Kenny (1986). Results: First, participants with high parental attachment showed high levels of school life adjustment, low level of depression, and low levels of relational aggression, demonstrating healthy psycho-social adjustment in adolescence. Second, paternal attachment played a facilitating role in the process of high maternal attachment's positive influence on school life adjustment. Third, paternal attachment mitigated the process of low maternal attachment's negative influence on depression. Conclusion: Our findings highlight the importance of emotional communication, support, and intimacy between parents and children in middle school. Furthermore, it emphasized the importance of the paternal role and involvement in the mother-child relationship.

• Korean Journal of Child Studies
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• v.34 no.2
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• pp.83-99
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• 2013

The premise underpinning this research is that cognitive evaluation is the starting point of children's emotional experience. It then went on to investigate how parents' person-oriented guidance affects children's emotional intelligence. Additionally, children's ego-resilience was analyzed as a variable that affects children's emotional intelligence and as a mediator between parents' person-oriented guidance and children's emotional intelligence. A purposive sampling was conducted in 4 elementary schools in metropolitan areas and made use of 466 students in 4th and 5th grade as research subjects. The results are summarized as follows：firstly, the more frequently parents used person-oriented guidance, the more children exhibited higher levels of ego-resilience and emotional intelligence. Secondly, vitality, peer relations, optimism, curiosity, emotional regulation, maternal person-oriented guidance were found to affect emotional intelligence in the order of relative significance of that influence. Thirdly, children's ego-resilience was found to intermediate parents' person-oriented guidance and children's emotional intelligence. In conclusion, individual children's evaluations of parental verbal stimuli was found to influence children's emotional intelligence, especially more significantly through the mediation of children's ego-resilience.

• Journal of the Korean Home Economics Association
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• v.47 no.1
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• pp.1-12
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• 2009

The purpose of this study was to establish the relative influences of individual variables (sex, age, temperament) and mother's psychosocial variables (maternal attitude, parenting stress, marriage satisfaction) on daily stress levels and maladjustment of young children attending at daycare centers. Subjects were 224 pairs of children and their mothers, and 18 teachers from 5 child-care centers in Gwangju City. Children were tested individually using 22-item daily stress inventory with illustrations which described certain stressful situations. Questionnaires were also administered to mothers and teachers. Collected data were subjected to Cronbach's $\alpha$, correlation and multiple regression analysis using SPSS (WIN 14.0) program. The major findings were as follows; Overall average score of children's daily stress was equivalent with the median. 'Blame and attack situation' and 'anxiety and frustrated situation' were perceived as more stressful for children than 'lose self-respect situation'. Overall average score of children's maladjustment was lower than the median. 'Overactivity' was highest among subscores of maladjustment. Age was the only significant influential factor on children's daily stress level. Sex was the most influential factor on 'overactivity', 'aggression', and 'regression' of young children. Activity and maternal stress were the second most influential factor on 'overactivity' and 'aggression', respectively.

• The Korean Journal of Community Living Science
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• v.21 no.3
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• pp.323-339
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• 2010

This study examines how family and school social capital moderate the relation between family income and academic achievement. I use the data from the Korean Educational and Employment Panel(KEEP) on the third year middle school students in 2004. Results show that higher levels of family and school social capital, as well as financial capital such as family income are more positively associated with academic achievement. In addition, family and school social capital are served as moderators of the influences of family income on academic achievement. For example, higher parental concern about children and teacher-student bonding provides an extra boost to the positive relationship between family income and student achievement. Furthermore, lower educational expectations can make the relation between income and achievement negative. These findings underscore the importance of social capital at home and at school as the alternatives to promote academic achievement. In particular, greater concern and support encouraging social capital at home and school should be directed at low-income students who are struggling with academic achievement.

• Journal of Labour Economics
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• v.31 no.1
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• pp.135-162
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• 2008

This paper analyzes the impact of the mother's employment on her time investment in children's career decisions. Parental conversations with high school children and the parents' effects on children's career decisions are used as the proxies for the quantity and quality of time investment, respectively. For the empirical analysis, IV ordered-probit regressions are fitted to the KLIPS data. Other things being equal, the mother's employment does not cast any statistically significant impact on the quantity and quality of time input for children in high school. The effect of mother's employment on her time investment in children is positive for professional jobs while negative for nonprofessional jobs, but both lack the weight of statistical significance.