• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.33-33
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• 2015

Clubroot disease is the major threat to the production of Chinese cabbage (Brassica rapa L.) in Japan. Although the breeding of the clubtoot resistant (CR) cultivars is one of the most efficient ways to control this disease, the CR cultivars do not always have effects due to the breakdown of resistance. Therefore, it is necessary to develop the breeding strategy to accumulate multiple CR genes in a single cultivar effectively. We have identified two incomplete dominant CR loci, Crr1 and Crr2, which are originated from the European CR turnip Siloga. To investigate the effectiveness of marker-assisted selection (MAS) for CR breeding, the inbred line with Crr1 and Crr2 was crossed with parental lines of the existing CR $F_1$ cultivar of Chinese cabbage, followed by 5 times of MAS and backcrossing. The $F_1$ derived from a cross between the resulting parental lines improved the clubroot resistance as expected and had the same morphological characters as the original $F_1$ cultivar. We have shown that the Crr1 locus comprised two loci: Crr1a, which by itself conferred resistance to the mild isolate; and Crr1b, which had a minor effect, but was not required for Crr1a-mediated resistance. Further genetic analysis suggested that Crr1b was necessary to acquire resistance to the more virulent isolate in combination with Crr2. Molecular characterization of Crr1a encoding TIR-NB-LRR class of R protein revealed that there were at least 4 alleles in Japanese CR cultivars of Chinese cabbage. PCR analysis with Crr1a-specific markers demonstrated that the functional alleles were predicted to be present in European CR turnips, Debra and 77b besides Siloga, whereas rarely in Japanese CR cultivars, indicating that Crr1a is an useful source to improve the resistance of Chinese cabbage cultivars.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.7
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• pp.3211-3217
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• 2013

Amid ever-increasing job-seeking burden and anxiety of college student, this study examined how their dysfunctional attitude affected job-seeking anxiety. The subjects of this study were 600 college students in Metropolitan area. The survey period was conducted from May 21, 2012 to June 1. The study had the findings as below. First, the impact of dysfunctional attitude on job-seeking anxiety was found in the form of 27% physical condition, 18% aroused situation and 10% parental expectations, and especially perfectionism had substantial impact on job-seeking anxiety. Second, correlation analysis between dysfunctional attitude and job-seeking anxiety showed that dysfunctional attitude had direct relationship with all of physical condition, aroused situation and parental expectations. Under the circumstances, more in-depth analysis of dysfunctional attitude and proper interventions are required so that students are able to lower their job-seeking anxiety, prepare healthy work life in society. So is required research and development of programs that can work as a driving force for students to minimize their dysfunctional aspects particularly pursuit of perfectionism and seek jobs as wanted.

• Journal of the Korea Society of Computer and Information
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• v.25 no.9
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• pp.159-166
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• 2020

The purpose of this research is to suggest some practical and policy implications for improving adolescent's self-esteem through analyzing longitudinally the effects of parental attitude, peer relationship, student-teacher relationship, school learning activity, life-goal on adolescent's self-esteem by using latent growth curve model. Analytical data was obtained from the KYCPS. The analytical results showed that the change model of adolescent's self_esteem turned out to be a leaner model in which the change rate and intercept were significant. Second, time dependent variables influence on adolescent's self_ esteem meaningfully. Third, time dependent variables influence on adolescent's self_ esteem changes significantly. This study proposed some policy implications basing on these findings.

• Journal of the Korean Home Economics Association
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• v.43 no.9 s.211
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• pp.71-84
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• 2005

The purpose of this study was firstly to identify how the characteristics of children(grades, birth order, self-consciousness), parents(age, educational level, employment status of mother, mother's parenting behavior), and context (private tutoring status, tying area) relate to children's stress and secondly to examine the relative effects of these variables on children's stress. The subjects of this study were 940(453 boys, 440 girls) children from grades 4 to 6 who were selected from three elementary schools in Seoul and Buchun. Data were analyzed with t-test, correlation, and multiple regression analysis. The results were as follows: (1) Girls get stressed easier than boys in terms of being treated distantly by their friends. (2) The study showed a negative relationship between children's stress and their self-consciousness such as general self-consciousness, teaming self-consciousness and total self-consciousness. (3) While the results showed a negative relationship between children's stress and mothers' acceptable behavior, the study revealed a positive relationship between children's stress and the mother's over protective behavior which in turn raised the children's stress level. (4) In 'total stress', it was recognized that boys were affected mostly tv grades in the children's variables category, while girls were affected by their general self-consciouness. In addition, both girls and boys were affected mostly by acceptable-refusal parental behavior in the parental variables category, and further affected by the living area in the category of conditional variables.

• The Journal of the Korea Contents Association
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• v.17 no.2
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• pp.1-12
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• 2017

This study investigated the effect of parental attachment on interpersonal competence and psychological well-being of college students. 625 college students completed self-administered questionnaires. Reliability test, ANOVA, Duncan ex-post analysis, and hierarchical regression analysis on data were used. Study results showed that parental attachement and interpersonal competence had significant effects on college students' psychological well-being. Results also depicted gender differences in the relationship of study variables; students' interpersonal competence demonstrated partial mediation effect in the relation of attachment with the same-sex parent and psychological well-being, while complete mediation effect in that of attachment with the opposite-sex parent and psychological well-being, These findings suggest that gender-conscious university policies to promote interpersonal competence among students are greatly needed to ensure psychological well-being of the student population.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4271-4274
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• 2014

The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.

• Journal of Digital Convergence
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• v.15 no.12
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• pp.59-70
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• 2017

The purpose of this study was to investigate whether there is a moderating effect of protective factors on risk factors in affecting school adjustment of immigrant youths. For this purpose, this study analyzed 69,720 youths who weighed 6,079 children of youths ages 9-24 among the 2015 National Survey of Multicultural Families. From the results of the analysis, first, the discrimination experience, school violence and depression, which are risk factors of immigrant youths, all had negative effects on school adjustment. Parental relations, self-esteem, Korean language ability, and nationality acquisition, which are protective factors, all had a positive effect on school adjustment. Second, among the three risk factors in the school adjustment of immigrant youths, self-esteem and nationality acquisition variables were analyzed as the protective factors moderating depression, and the protective factors controlling school violence were analyzed as parental relationship, self-esteem, Korean ability and nationality acquisition variables. Based on the results of the analysis, decrease in immigrant youths' depression, coping with academic violence, and development and support of various competency development programs were suggested as major social welfare practices.

• Korean Journal of Community Nutrition
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• v.3 no.4
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• pp.542-555
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• 1998

This study was conducted to assess the nutrient intake patterns among urban and rural adolescents and to investigate the effects due to parent's socioeconomic status and other factors, such as mother's job, family type and regular exercise on that pattern. 2,455 middle and high school students living in Seoul and Yangpong, Kyounffi-Do participated in a self-administered questionnaire that was used to collect data. The one-day dietary intake was surveyed through a 24-hour recall method. The factors significantly different between urban and rural adolescents according to monthly income, parent's education level, mother's job, family type and exercise. Income, the parents' education level and regular exercise were associated with the patterns of nutrient intakes as a percent of the RDA. So, when adjusted for parental income, the father's and mother's education level and regular exercise, there were no signifcant differences within the patterns of nutritional intake between urban and rural adolescents. The results provided the information regarding the determinants of nutrient status among adolescents and were expected to be helpful for planning school health promotion programs.

• Nutrition Research and Practice
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• v.7 no.4
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• pp.281-286
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• 2013

We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FR${\alpha}$), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented, PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: $PS{\times}MS$, $PS{\times}MD$, $PD{\times}MS$, and $PD{\times}MD$. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the $PD{\times}MD$ and $PS{\times}MD$ and $PD{\times}MS$ as compared with the $PS{\times}MS$ group. The hepatic global DNA methylation was decreased in the $PD{\times}MS$ and $PS{\times}MD$ groups as much as in the $PD{\times}MD$ group, and all the three groups were significantly lower as compared to the $PS{\times}MS$ group. There were no significant differences in the hepatic FR${\alpha}$, IGF-2 and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FR${\alpha}$, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.

• Genomics & Informatics
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• v.19 no.1
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• pp.8.1-8.12
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• 2021

Cytorace-3 is a laboratory evolved hybrid lineage of Drosophila nasuta nasuta males and Drosophila nasuta albomicans females currently passing ~850 generations. To assess interracial hybridization effects on gene expression in Cytorace-3 we profiled the transcriptomes of mature ovaries and testes by employing Illumina sequencing technology and de novo transcriptome assembling strategies. We found 26% of the ovarian, and 14% of testis genes to be differentially expressed in Cytorace-3 relative to the expressed genes in the parental gonadal transcriptomes. About 5% of genes exhibited additive gene expression pattern in the ovary and 3% in the testis, while the remaining genes were misexpressed in Cytorace-3. Nearly 772 of these misexpressed genes in the ovary and 413 in the testis were either over-or under-dominant. Genes following D. n. nasuta dominance was twice (270 genes) than D. n. albomicans dominance (133 genes) in the ovary. In contrast, only 105 genes showed D. n. nasuta dominance and 207 showed D. n. albomicans dominance in testis transcriptome. Of the six expression inheritance patterns, conserved inheritance pattern was predominant for both ovary (73%) and testis (85%) in Cytorace-3. This study is the first to provide an overview of the expression divergence and inheritance patterns of the transcriptomes in an independently evolving distinct hybrid lineage of Drosophila. This recorded expression divergence in Cytorace-3 surpasses that between parental lineages illustrating the strong impact of hybridization driving rapid gene expression changes.