Genomics & Informatics

Korea Genome Organization (한국유전체학회)
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De novo assembly, annotation and gene expression profiles of gonads of Cytorace-3, a hybrid lineage of Drosophila nasuta nasuta and D. n. albomicans

  • Ponnanna, Koushik (Department of Studies in Genetics and Genomics, University of Mysore) ;
  • DSouza, Stafny M. (Department of Studies in Genetics and Genomics, University of Mysore) ;
  • Ramachandra, Nallur B. (Department of Studies in Genetics and Genomics, University of Mysore)
  • Received : 2020.09.07
  • Accepted : 2020.12.19
  • Published : 2021.03.31
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Abstract

Cytorace-3 is a laboratory evolved hybrid lineage of Drosophila nasuta nasuta males and Drosophila nasuta albomicans females currently passing ~850 generations. To assess interracial hybridization effects on gene expression in Cytorace-3 we profiled the transcriptomes of mature ovaries and testes by employing Illumina sequencing technology and de novo transcriptome assembling strategies. We found 26% of the ovarian, and 14% of testis genes to be differentially expressed in Cytorace-3 relative to the expressed genes in the parental gonadal transcriptomes. About 5% of genes exhibited additive gene expression pattern in the ovary and 3% in the testis, while the remaining genes were misexpressed in Cytorace-3. Nearly 772 of these misexpressed genes in the ovary and 413 in the testis were either over-or under-dominant. Genes following D. n. nasuta dominance was twice (270 genes) than D. n. albomicans dominance (133 genes) in the ovary. In contrast, only 105 genes showed D. n. nasuta dominance and 207 showed D. n. albomicans dominance in testis transcriptome. Of the six expression inheritance patterns, conserved inheritance pattern was predominant for both ovary (73%) and testis (85%) in Cytorace-3. This study is the first to provide an overview of the expression divergence and inheritance patterns of the transcriptomes in an independently evolving distinct hybrid lineage of Drosophila. This recorded expression divergence in Cytorace-3 surpasses that between parental lineages illustrating the strong impact of hybridization driving rapid gene expression changes.

Keywords

Acknowledgement

We thank the Department of Studies in Genetics and Genomics, the University of Mysore for providing the infrastructure. We thank Dr. Amruthavalli C. and Dr. M.S. Ranjini (DBT project co-investigators) for the support. We thank Rajanikanth C. for generous help with the illustrations. This work was supported by Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (grant number BT/PR9871/BID/7/472/2013) to NBR; DST-KSTePS Fellowship (LIF-02) from Karnataka Science and Technology Promotion Society to K.P and DST-INSPIRE Fellowship (IF150898) from Department of Science and Technology, Government of India to S.M.D.

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