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General Oceanographic Factors In Yeongil Bay Of Korea, Late October 1973 (가을철 영일만수괴의 일반해양학적 특성)

  • Kwak, Hi-Sang
    • 한국해양학회지
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    • v.11 no.2
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    • pp.89-95
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    • 1976
  • Some factors of seawater such as water temperature, salinity, pH, dissolved oxygen and asturation of dissolved oxygen were determined in 5 meters intervals from surface to bottom at 11 stations in Yeongil Bay of Korea during late October 1973. Distribution pattern of water masses in Yeongil Bay during the period seemed to be heterogeneous as dividing into two parts of surface and bottom. Water temperature, pH, dissolved oxygen and saturation of dissolved oxygen of surface water mass showed higher values and salinity of the mass showed lower values than those factors of bottom water mass. Surface water mass might be originated from Tsushima current during summer season and bottom water mass from cold body of East Sea of Korea which seemed to extend to coastal zone during winter season. Land water discharge from Hyeongsan River into the Bay is considered as a minor factor playing slight role in the water mass composition of the area.

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A Study on the Decomposition of Litter and the Leaching of Mineral Nutrients in the Stands of Pinus rigida on Mt. Gwan-ak and Pseudosasa japonica on Odong-do (관악산의 리기다소나무림과 오동도의 이대림에서 낙엽의 분해와 무기양분의 세탈에 관한 연구)

  • Chang, Nam-Kee;Heui-Baik Kim;Jun-hi Yoo
    • The Korean Journal of Ecology
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    • v.9 no.1
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    • pp.51-58
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    • 1986
  • The decay rates of organic matter were investigated and the leaching rates were determined from the concentration distribution of N,P,K, Ca and Na in the soil profile at Pinus rigida stand on Mt. Gwan-ak and at Pseudosasa japonica stand on Odong-do. In order to determine the leaching rates θo/W wazs substituted with KL in Towner's equation. Decay rates were 0.191 at Pinus stand on Mt. Gwan-ak and 0.234 at Pseudosasa stan don Odong-do. Concentration distributions of N,P, K and Na in soil profiles were corresponed with Towner's model curve. Leaching rates determined from concentration distributions were 0.086, 0.079, 0.041, 0.029, 0.096 on Mt. Gwan-ak and 0.080, 0.056, 0.051, 0.008, 0.028 on Odong-do. The soil of Pseudosasa stand showed on the whole lower leaching rates than that of Pinus stand. The soil of Pseudosasa stand showing higher decay rate and lower leaching rates contained more concentration of each nutrient than that of Pinus stand.

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Cloning and Expression of the Structural Gene for Alcohol Dehydrogenase of Zymomonas mobilis in Escherichia coli (Zymomonas mobilis 알코올 탈수소 효소 유전자의 Cloning과 Escherichia coli 에서의 발현)

  • Yoon, Ki-Hong;Shin, Byung-Sik;M.Y Pack
    • Microbiology and Biotechnology Letters
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    • v.17 no.4
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    • pp.301-306
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    • 1989
  • A genomic library of Zymomonas mobilis DNA was constructed in Escherichia coli using plasmid pUC9 Allyl alcohol was used to screen a genomic clone expressing alcohol dehydrogenase. The plasmids isolated from two clones, which were sensitive to allyl alcohol, were found to be related and to share a common 2.6 kb fragment encoding alcohol dehydrogenase II identified as one of two isozymes in Z. mobilis by staining for alcohol dehydrogenase activity on polyacrylamide gel and spectrophotometric analysis of several substrate oxidations.

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The Effects of a Dementia Education Program for the Aged (일반 재가노인을 위한 치매교육 프로그램의 효과)

  • Kong, Eun-Hi;Jeong, Young-Soon
    • Research in Community and Public Health Nursing
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    • v.22 no.3
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    • pp.252-261
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    • 2011
  • Purpose: This study was conducted to evaluate the effects of a dementia education program for Korean older adults who resided in community. Methods: The research adopted the non-equivalent control group pretestposttest design. A total of 66 older adults completed the study (31 older adults in the experimental group and 35 in the control group). The Experimental group participated in a six-session dementia education program for three weeks. The effects of the dementia education program were evaluated using the measures of knowledge of dementia and attitude toward dementia. Data were analyzed using independent sample t-test with SPSS/WIN 18.0. Results: There was a significant change in the knowledge of dementia in the experimental group (t=2.75, p=.008). Although there was an increase in attitude toward dementia in the experimental group, the change was not statistically significant (t=1.26, p=.212). Conclusion: The results indicated that the dementia education program is effective in improving the knowledge of dementia among community-dwelling older adults. There is a need to develop more effective dementia education programs designed to improve older adults' attitude toward dementia. Further research is needed using a highly rigorous research methodology.

Chemically Enhanced Primary Treatment at D Wastewater Treatment Plant (화학적 처리에 의한 1차 하수처리장의 처리효과 개선 -현장시험을 중심으로-)

  • Kwak, Jong W.;Kim, Seung H.;Lee, Chan W.
    • Journal of Korean Society of Water and Wastewater
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    • v.11 no.1
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    • pp.99-108
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    • 1997
  • This study examined the feasbility of upgrading D waste water treatment plant which treats incoming wastewater by primary sedimentation only. By adding 20-40 ppm of Hi-PAX into the outlet of the aerated grit chamber, BOD and SS removal efficiences were improved from 29% and 36% to 53 % and 73%,respectively. However, chemically enhanced primary treatment failed to meet the upcoming wastewater quality standard(BOD and SS <20 mg/l) consistently. This was suspected to result from the deteriorated plant return stream. The wastewater treatment by chemical treatment should have increased the amount of the sludge to be removed as the sludge production was increased. Chemically enhanced primary treatment is anticipated to consistently produce effluent of the 1996 standards quality by adjusting the amount of the sludge to be removed. Besides BOD and SS removal, chemically enhanced primary treatment resulted in the improved T-P removal from 30% to 64-74%. However, such benefit was not observed in T-N removal. Improved T-P removal will be expected to help control water pollution in Masan bay.

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Piezoelectric and Dielectric Properties of PMN-PNN-PZT Ceramics for Ultrasonic Generator with Calcination Temperature (하소온도변화에 따른 초음파절삭기용 PMN-PNN-PZT 세라믹스의 압전 및 유전 특성)

  • Yoo, Ju-Hyun;Kim, Seung-Won;Seo, Dong-Hi;Lee, Eun-Sup;Choi, Nak-Gu;Jeong, Hoy-Seung
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.30 no.3
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    • pp.152-156
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    • 2017
  • In this paper, $Pb(Mn_{1/3}Nb_{2/3})_{0.07}(Ni_{1/3}Nb_{2/3})_{0.10}(Zr_{0.5}Ti_{0.5})_{0.83}O_3$ ceramics were fabricated by the conventional solid state method to obtain excellent dielectric properties for ultrasonic generators. The effects of 2nd calcination temperature on their microstructure and piezoelectric properties were systematically investigated. The tetragonality increased in the ceramics when 2nd calcination temperature increased to the optimized temperature at $750^{\circ}C$. At that temperature, excellent physical properties ($d_{33}=352\;pC/N$, ${\varepsilon}_r=1,687$, $k_p=0.570$, $Q_m=1,640$) were obtained for ultrasonic generator application.

Characterization of a Bacteriocin Produced by Enterococcus sp. T7 Isolated from Humans

  • Moon, Hi-Seong;Jeong, Jong-Jin;Ji, Geun-Eog;Kim, Jong-Sang;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.507-513
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    • 2000
  • A bacteriocin-producing organism, Enterococcus sp. T7, was isolated from human fecal samples. Bacteriocin T7, named tentatively as the bacteriocin, was produced by Enterococcus sp. T7 and it inhibited some strains of Lactobacillus. Staphylococcus, Enterococcus, and Streptococcus, but not all the lactococci and gram-negative bacteria tested. Bacteriocin T7 inhibited the growth of Listeria monocytogenes Scott A, but the degree of inhibition was less than those for other sensitive gram-positive vacteria. Bacteriocin T7 in MRS broth started to produce at the middle of the exponential growth phase and the inhibitory activity reached its maximum level during the stationary growth phase. Bacteriocin T7 was stable against heat treatments, pH variations (pH 2-10), and exposure to organic solvents. The molecular weight of bacteriocin T7 was estimated to be 6.500 Da by SDS-PAGe. All these facts, including physico-chemical stabilities, small molecular size, and inhibition of Kisteria monocytogenes, indicate that bacteriocin T7 is likely to be a member of the class IIa bacteriocins.

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Purification and Characterization of a Thermostable ${\beta}-1$,3-1,4-Glucanase from Laetiporus sulphureus var. miniatus

  • Hong, Mi-Ri;Kim, Yeong-Su;Joo, Ah-Reum;Lee, Jung-Kul;Kim, Yeong-Suk;Oh, Deok-Kun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.8
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    • pp.818-822
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    • 2009
  • A ${\beta}-1$,3-1,4-glucanase from the fungus Laetiporus sulphureus var. miniatus was purified as a single 26 kDa band by ammonium sulfate precipitation, HiTrap Q HP, and UNO Q ion-exchange chromatography, with a specific activity of 29 U/mg. The molecular mass of the native enzyme was 52 kDa as a dimer by gel filtration. ${\beta}-1$,3-1,4-Glucanase showed optimum activity at pH 4.0 and $75^{\circ}C$. The half-lives of the enzyme at $70^{\circ}C$ and $75^{\circ}C$ were 152 h and 22 h, respectively. The enzyme showed the highest activity for barley ${\beta}$-glucan as ${\beta}-1$,3-1,4-glucan among the tested polysaccharides and p-nitrophenyl-${\beta}$-D-glycosides with a $K_m$, of 0.67 mg/ml, a $k_{cat}$ of 13.5 $S^{-1}$ and a $k_{cat}/K_m$ of 20 mg/ml/s.

Cloning, Analysis, and Expression of the Gene for Thermostable Polyphosphate Kinase of Thermus caldophilus GK24 and Properties of the Recombinant Enzyme

  • Hoe, Hyang-Sook;Lee, Sung-Kyoung;Lee, Dae-Sil;Kwon, Suk-Tae
    • Journal of Microbiology and Biotechnology
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    • v.13 no.1
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    • pp.139-145
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    • 2003
  • The gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and $39\%$ to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with $36\%$ yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and $70^{\circ}C$, respectively. A divalent cation was required for the enzyme activity, with $Mg^2+$ being the most effective.

The Effect of Advanced Clinical Practicum on Clinical Competence for Nursing Student (종합선택실습이 간호대학생의 임상실무수행능력에 미치는 영향)

  • Kim, Jisoo;Kim, Young-Sook;Kim, Hye Soon;Jo, Hyun Sook;Lee, Eun-Ja;Lee, Yeo Jin;Kim, Yunmi;Park, Kwang-Hi
    • Proceedings of the Korea Contents Association Conference
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    • 2011.05a
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    • pp.381-382
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    • 2011
  • 본 연구는 4주간의 종합선택실습이 간호대학생의 임상수행 능력 변화에 미치는 영향을 파악하기 위한 것으로 4주간의 종합선택실습을 이수한 4학년 간호 대학생 128을 대상으로 하였고 임상실무수행능력 평가도구를 이용하여 실무능력변화를 측정하였다. 연구 결과 종합선택실습 전의 임상실무능력이 70.4점에서 실습 후에는 124.8점으로 증가(p<.001)되었고 직접간호활동과 간접간호활동의 모든 하위 영역에서 실습 후의 임상실무수행능력이 유의하게 증가되었다. 또한 상위 성적 30% 학생의 종합선택실습 후의 임상실무수행능력이 중간 40%의 학생에 비해 더 유의하게 증가한 것으로 나타났다(p=.047).

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