• Journal of Embryo Transfer
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• v.28 no.1
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• pp.7-12
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• 2013

Body condition score (BCS) is a useful management tool for distinguishing differences in nutritional needs of cows in the herd. Although it is not always possible to quantify the nutrient content of the feed supplied to the donor cow, the nutritional status can be determined by the BCS. The objective of this study was to evaluate in vivo embryo production, return to estrous of donor and pregnancy rate of recipients following BCS in Hanwoo superovulation. Sixty nine Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with Embryo Collection Medium. The results obtained were as follows: No differences were observed in the efficiency of superovulation rates regardless of BCS ($${\leq_-}2.0$$, 2.5 to 3.0, and $${\geq_-}3.5$$). The mean number of total embryos was each $5.20{\pm}0.86$, $11.56{\pm}1.04$, and $6.23{\pm}1.07$. The mean number of transferable embryo from $${\leq_-}2.0$$, 2.5 to 3.0, and $${\geq_-}3.5$$ of BCS was $2.60{\pm}0.87$, $7.94{\pm}0.89$, and $4.75{\pm}1.32$, respectively (p<0.05). Return to estrous regardless of donor BCS was no difference. The pregnancy rates of recipient were BCS $${\leq_-}2.0$$ 11.76%, 2.5 to 3.0 40.79%, and $${\geq_-}3.5$$ 11.11%, following transfer of fresh embryos produced in vivo, respectively. These results indicate that if the Hanwoo with BCS 2.5 to 3.3 are used for donor and recipient, the embryo production and the conception rate will be greater.

• Korean journal of food and cookery science
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• v.7 no.4
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• pp.35-40
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• 1991

The sensory evaluation by Preference test revealed that the best Kongjook was obtained when the soymilk was prepared with eight-times of water to the volume of soybean. Based on the results, the ratios of soybean: rice: water (v/v/v) having 2:1:16($G_1$), 3:2:24($G_2$), 3:2:30($G_3$),and 4:3:32($G_4$), were chosen. The soaked rice was cooked with soymilk, Kongjook prepared from $G_2$ and $G_4$, showed the highes preference in eating quality and flavor, but the former had better quality in consistency and color, indicating that Kongjook from $G_2$ was the most preferable. The cooking time of soybean prior to the preparation of soymilk also showed that Kongjook prepared from $G_2$ had better preference than other samples regardless of the cooking times of soybean. The spreadability of Kongjook from $G_2$ determined with a line-chart method was 5.85.

• Korean journal of food and cookery science
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• v.28 no.6
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• pp.711-719
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• 2012

The collagens from squid (Todarodes pacificus) skin and Alaska pollack (Theragra chalcogramma) skin were extracted and their physicochemical properties were investigated. The yield was improved with the increase of NaOH concentration and was increased Neutrase than Alcalase in enzymatic hydrolysates. Protein and collagen contents from Alaska pollack skin were 38.3~62.7% and 13.1~28.9%, respectively. All enzymatic hydrolysates also showed high antioxidant activities as NaOH concentration decrease. Composition of their amino acids was mainly glycine and proline. The spectrum of FT-IR of the collagen showed wavenumber at $1,631cm^{-1}$, $1,549cm^{-1}$, $1,234cm^{-1}$ and $3,322cm^{-1}$ representing the regions of amide I, amide II, amide III and amide A, respectively. The decomposition temperature for the collagen was in the range of $300^{\circ}C$ and showed relatively good enough for their thermal stabilities.

• Korean journal of food and cookery science
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• v.28 no.5
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• pp.559-567
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• 2012

The purpose of this study was to investigate the perceptions of the foodservice industry workers in regard to the use of seasonings and sweeteners in the restaurants. Questionnaires were distributed to 902 foodservice workers of various restaurants. Participants were questioned on their perceptions of both natural and artificial seasonings & sweeteners. Furthermore, they were asked to respond to questions regarding the need of public education and information about the use of seasonings and sweeteners, along with the demographic information. The results showed that 65.7% of respondents used both the artificial seasoning and artificial sweeteners, while 9.9% of respondents used only natural seasonings and sweeteners. The restaurant employees answered that they have tried to reduce the use of artificial seasonings and sweeteners(3.69/5 point). Some of the respondents reported that they have tried to increase the used of natural seasonings and sweeteners(3.54/5 point). There were significant differences in the respondents' perceptions and attitudes on the seasonings and sweeteners by the restaurant type(p<0.05). The respondents perceived the need for education on the safety of artificial seasonings and sweeteners(3.71/5point) and the production process of natural seasonings(3.75/5 point). There was no significant difference in the respondents' perceptions on the need for education of the seasonings and sweeteners by the restaurant type. The findings suggest that education regarding the use of artificial seasoning and artificial sweeteners, as well as cooking methods that use natural seasonings, is needed to reduce the use of artificial seasonings and sweeteners in restaurants.

• Korean journal of food and cookery science
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• v.28 no.5
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• pp.605-612
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• 2012

In comparison of the antioxidant activities by the different extraction methods of the domestic mung bean varieties, 'Geumsung', 'Dahyeon', 'Sohyeon', and 'Eohul', were soaked and dehulled, with the extracts obtained by using 80% ethanol at room temperature or heated. The mung bean hull was freeze-dried, ground, and passed through 100 mesh sieve. The moisture content of the hull powder ranged from 1.70 to 3.15%. The total dietary fiber content ranged from 84.42 to 88.47%, with the Sohyeon hull showing the highest value. The L value of Geumsung hull (62.93) was the highest, and the a value of Sohyeon hull (-1.69) was the lowest (p<0.05). The color difference was the highest in Dahyeon hull (42.52) and lowest in Geumsung hull (38.52). The Eohul hull's chlorophyll a and b contents were the highest with the 80% ethanol extract by heating (10.55 and 6.12 ${\mu}g/mL$, respectively), but the Sohyeon hull showed the highest in 80% ethanol extract at room temperature (7.63 and 3.93 ${\mu}g/mL$, respectively). The total phenolic and total flavonoid contents of the ethanol extracts were significantly different between the varieties regardless of the extraction temperature. The hull extract from the heating extraction was higher in Eohul than in other varieties, and was the lowest in the Dahyeon variety. The extract from Sohyeon hull at room temperature showed the highest contents of total phenolic and flavonoid contents. In the mung bean hull extract by heating, DPPH and ABTs radical scavenging activity ranged from 13.49-16.75% and 22.93-39.42% respectively and those at room temperature ranged from 39.86-41.88% and 49.71-70.92%. The reducing powers of extracts by heating ranged from 7.29 to 9.36 mg BHT/g hull, and at room temperature, they ranged from 19.90 to 20.97 mg BHT/g hull. Our results indicate that different extraction methods influenced antioxidant activities in the domestic mung bean varieties.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.262-268
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• 2001

Two commercial wood vinegar liquors prepared from Cryptomeria japonica and Quercus sp., which are used as a mineral fertilizer in Korea, were extracted using dichloromethane as a solvent, respectively. The extracts were separated into acidic, phenolic, neutral and basic fraction by acid or alkali treatment, and the compositions of each fraction were analyzed by means of GC and GC-MS. A total of 103 compounds including 26 acids, 32 phenols and 45 neutral compounds were identified. The major components were acetic, propionic and n-butyric acid, representing of $41{\sim}58%$ of the acidic fraction, guaiacol, 4-methylguaiacol and phenol, repersenting of $53.2{\sim}63.9%$ of the phenolic fraction, and furfural, 3-methyl-2-cyclopenten-2-one, 2,3-dimethyl-2-cyclopenten-1-one and 5-methyl-2-furfural in the neutral fraction. In addition to these compounds, phenolic fraction in dichloromathane extract from wood vinegar liquor of C. japonica included large amounts of vanillin, acetovanillone and tentatively identified ethylvanillyl ether while that of Quercus sp. included some amounts of syringol and 4-methylsyringol.

• Korean Journal of Environmental Agriculture
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• v.39 no.1
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• pp.75-82
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• 2020

BACKGROUND: This study was carried out to establish pre-harvest residue limits (PHRLs) of indoxacarb and pymetrozine in broccoli under greenhouse conditions, based on dissipation patterns and biological half-lives of pesticides during 10 days after application. METHODS AND RESULTS: The field studies were conducted in two different greenhouse, located in Chungju-si (Field 1) and Gunsan-si (Field 2). Samples were collected at 0, 1, 2, 3, 5, 7 and 10 days after spraying pesticide suspension. The analytical methods for indoxacarb and pymetrozine using HPLC-DAD were validated by recoveries ranging of 94.3-105.4% and 81.8-96.0%, respectively, and MLOQ (Method Limit of Quantification) of 0.05 mg/kg. Biological half-lives of indoxacarb and pymetrozine were 2.9 and 3.2-3.8 days in broccoli, respectively. The lower 95% confidence intervals of dissipation rate constant of indoxacarb were determined as 0.1508 (Field 1) and 0.2017 (Field 2), whereas those of pymetrozine were calculated as 0.1489 (Field 1) and 0.1577 (Field 2). CONCLUSION: The significant differences were not observed between the dissipation rates of indoxacarb and pymetrozine in broccoli. The major factor affecting residue dissipation was the dilution effect by fast growth. The PHRLs for 10 days prior to harvest were recommended as 30.06 (Field 1) and 18.07 (Field 2) mg/kg for indoxacarb, and 4.84 (Field 1) and 4.43 (Field 2) mg/kg for pymetrozine, respectively.

• Journal of Preventive Medicine and Public Health
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• v.37 no.4
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• pp.381-389
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• 2004

Objective : To compare the efficacy between SKI306X and Diclofenac by using generalized estimating equations (GEE) methodology in the analysis of correlated bivariate binary outcome data in Osteoarthritis (OA) diseases. Methods : A randomized, double-blind, active comparator-controlled, non-inferiority clinical trial was conducted at 5 institutions in Korea with the random assignment of 248 patients aged 35 to 75 years old with OA of the knee and clinical evidence of OA. Patients were enrolled in this study if they had at least moderate pain in the affected knee joint and a score larger than 35mm as assessed by VAS (Visual Analog Scale). The main exposure variable was treatment (SKI 306X vs. Diclofenac) and other covariates were age, sex, BMI, baseline VAS, center, operation history (Yes/No), NSAIDS (Y/N), acupuncture (Y/N), herbal medicine (Y/N), past history of musculoskeletal disease (Y/N), and previous therapy related with OA (Y/N). The main study outcome was the change of VAS pain scores from baseline to the 2nd and 4th weeks after treatment. Pain scores were obtained as baseline, 2nd and 4th weeks after treatment. We applied GEE approach with empirical covariance matrix and independent(or exchangeable) working correlation matrix to evaluate the relation of several risk factors to the change of VAS pain scores with correlated binary bivariate outcomes. Results : While baseline VAS, age, and acupuncture variables had protective effects for reducing the OA pain, its treatment (Joins/Diclofenac) was not statistically significant through GEE methodology (ITT:aOR=1.37, 95% CI=(0.8200, 2.26), PP:aOR=1.47, 95% CI=(0.73, 2.95)). The goodness-of-fit statistic for GEE (6.55, p=0.68) was computed to assess the adequacy of the fitted final model. Conclusions : Both ANCOVA and GEE methods yielded non statistical significance in the evaluation of non-inferiority of the efficacy between SKI306X and Diclofenac. While VAS outcome for each visit was applied in GEE, only VAS outcome for the fourth visit was applied in ANCOVA. So the GEE methodology is more accurate for the analysis of correlated outcomes.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.441-445
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• 2005

태화강 주변지역 지하수의 계절적 수질 변화를 보고자 현장측정 항목(수온, pH, 알칼리도, 전기전도도), 일반 항목(증발잔류물, 총경도, 과망간산칼륨소비량), 음이온물질$(F^-,\;Cl^-,\;{NO_3}^-,\;{SO_4}^{2-})$, 양이온물질$(Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+)$등으로 구분하여 분석하였으며, 총 70개 지점을 대상으로 2004년 5월, 10월 2회에 걸쳐 실시하였다. 지역별 수질특성을 살펴보면 남구지역은 $Ca-Mg-HCO_3,\;Ca-Mg-HCO_3-Cl,\;Ca-Mg-Na-Cl-HCO_3,\;Na-Cl-HCO_3$의 4가지 유형이 전체 시료의 74%를 차지하였으며, $Ca-Mg-HCO_3$형이 가장 우세하게 나타났고, 중구지역 지하수에서는 $Ca-Mg-HCO_3-Cl,\;Ca-Mg-Na-HCO_3-Cl,\;Ca-Mg-HCO_3$의 3가지 유형이 전체 시료의 60%를 차지하는 것으로 나타났으며, 이중에서 $Ca-Mg-HCO_3-Cl$형이 가장 우세하게 나타났다. 두 지역의 수질 변화를 살펴보면. 전기전도도는 남구는 $731.5{\mu}s/m^3$에서 $529.8{\mu}s/m^3$, 중구는 $752.6{\mu}s/m^3$에서 $621.6{\mu}s/m^3$로 201.7, $131.0{\mu}s/m^3$만큼 작아져 두 지역 모두 같은 양상을 보였으나, Hardness 및 TDS의 경우 남구지역은 5월보다 10월에 평균, 최대값이 모두 낮게 나타났다. 또한 $Cl^-$의 경우 지역적, 계절적으로 큰 차이를 보이고 있으며 남구는 5월 68.2mg/l, 10월에는 61.7mg/l로 다소 감소하였으나 중구의 경우 5월 75.5mg/l, 10월 122.1mg/l로 다소 증가한 것으로 나타났다. 양이온 분포 비율 및 농도는 비슷하게 나타났으며, 계절적으로 5월보다 10월에 모두 높게 나타났다. 두 지역 모두 양이온물질 중 나트륨의 분포비율 및 농도가 5월보다 10월에 다소 높게 나타났다. 연구 지역 지하수의 계절적 수질변화를 살펴보면 두 지역간의 지하수질 및 분포특성에 있어 다소 차이를 보이고 있으며, 특히 중구 지역에서 5월보다 10월에 나트륨과 염소이온의 증가가 다소 나타나는 것으로 관찰되었다. 또한 연구지역 중 특이지점($Cl^-$:1,000mg/l이상)은 남구는 5월에 2개에서 10월에는 3개 지점으로 증가하였으며, 중구는 5월, 10월 모두 4개 지점으로 나타났다.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.2
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• pp.228-235
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• 2010

This study was performed to investigate the memory enhancing effect of Poria cocos Wolf (Hoelen cum radix) against scopolamine-induced amnesia in Sprague-Dawley (SD) rats. To induce amnesia, scopolamine (0.75 mg/kg) was intraperitonically injected into SD rats 30 min before starting behavior tests. We have conducted Morris water-maze and Y-maze tests to monitor learning and memory functions. Poria cocos effectively reversed scopolamine-induced memory impairment in SD rats which was represented by an improvement of mean escape latency in water-maze test and spontaneous alterations in Y-maze test. To elucidate possible molecule mechanism, we have measured mRNA as well as protein expression of acetylcholine esterase (AchE), choline acetyltransferase (ChAT), muscarinic acetylcholine receptor (mAchR), and brain-derived neurotrophic factor (BDNF) using RT-PCR and Western blot analysis, respectively. Poria cocos increased mRNA levels of ChAT and mAchR in rat hippocampus compared with those in the scopolamine-injected amnesic group. In addition, protein expression of ChAT and BDNF was also elevated by Poria cocos intake. Furthermore, as an upstrem regulator, the activation of cAMP response element-binding protein (CREB) was assessed by immunohistochemistry. In this immunohistochemical analysis, the phosphorylation of CREB (p-CREB) was reduced by scopolamine injection, which was restored back to control levels by administration of Poria cocos. These results suggest that Poria cocos may improve memory and cognitive deficit in amnesia and have therapeutic potentials through up-regulation of ChAT, mAchR, and BDNF, which seemed to be mediated by activation of CREB.