Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Residue Dissipation Patterns of Indoxacarb and Pymetrozine in Broccoli under Greenhouse Conditions

시설재배 브로콜리 중 Indoxacarb 및 Pymetrozine의 잔류 소실특성

  • Yang, Seung-Hyun (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University) ;
  • Lee, Jae-In (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University) ;
  • Choi, Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University)
  • 양승현 (원광대학교 농식품융합대학 생물환경화학과) ;
  • 이재인 (원광대학교 농식품융합대학 생물환경화학과) ;
  • 최훈 (원광대학교 농식품융합대학 생물환경화학과)
  • Received : 2020.02.21
  • Accepted : 2020.03.26
  • Published : 2020.03.31
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Abstract

BACKGROUND: This study was carried out to establish pre-harvest residue limits (PHRLs) of indoxacarb and pymetrozine in broccoli under greenhouse conditions, based on dissipation patterns and biological half-lives of pesticides during 10 days after application. METHODS AND RESULTS: The field studies were conducted in two different greenhouse, located in Chungju-si (Field 1) and Gunsan-si (Field 2). Samples were collected at 0, 1, 2, 3, 5, 7 and 10 days after spraying pesticide suspension. The analytical methods for indoxacarb and pymetrozine using HPLC-DAD were validated by recoveries ranging of 94.3-105.4% and 81.8-96.0%, respectively, and MLOQ (Method Limit of Quantification) of 0.05 mg/kg. Biological half-lives of indoxacarb and pymetrozine were 2.9 and 3.2-3.8 days in broccoli, respectively. The lower 95% confidence intervals of dissipation rate constant of indoxacarb were determined as 0.1508 (Field 1) and 0.2017 (Field 2), whereas those of pymetrozine were calculated as 0.1489 (Field 1) and 0.1577 (Field 2). CONCLUSION: The significant differences were not observed between the dissipation rates of indoxacarb and pymetrozine in broccoli. The major factor affecting residue dissipation was the dilution effect by fast growth. The PHRLs for 10 days prior to harvest were recommended as 30.06 (Field 1) and 18.07 (Field 2) mg/kg for indoxacarb, and 4.84 (Field 1) and 4.43 (Field 2) mg/kg for pymetrozine, respectively.

본 연구에서는 시설재배 하는 브로콜리 중 indoxacarb 및 pymetrozine의 경시적 잔류변화를 조사하여 생물학적 반감기와 감소상수를 산출하여 생산단계 잔류허용기준(PHRL)을 설정하고자 하였다. 포장시험은 충주시(포장 1) 및 군산시(포장 2)에 소재한 시설재배지에서 수행되었으며 약제살포 후 0, 1, 2, 3, 5, 7, 10일차에 시료를 채취하여 분석하였다. 브로콜리 중 잔류량은 HPLC-DAD로 분석하였으며, indoxacarb 및 pymetrozine의 회수율은 각각 94.3~105.4% 및 81.8~96.0%이었으며, MLOQ (Method Limit of quantitation)는 모두 0.05 mg/kg이었다. 브로콜리 중 indoxacarb 및 pymetrozine의 생물학적 반감기는 각각 2.9일, 3.2~3.8일이었으며, 감소상수의 95% 신뢰수준 하한치는 indoxacarb 0.1508 및 0.2017, pymetrozine 0.1489 및 0.1577로써 포장별, 약제별 유의적 차이(p<0.05)가 없었다. 브로콜리 중 농약 잔류량 소실특성의 주요 요인은 증체로 인한 희석효과이었으며, 브로콜리의 수확 10일전 PHRL은 indoxacarb의 경우 30.06(포장 1) 및 18.07(포장 2) mg/kg이었고, pymetrozine은 4.84(포장 1) 및 4.43(포장 2) mg/kg이었다.

Keywords

References

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