DOI QR코드

DOI QR Code

The Antioxidant Activities of the Korean Variety Mung Bean Hull Extracts as Dependent on the Different Extraction Methods

국내산 품종의 녹두껍질 추출물의 산화방지활성에 미치는 추출방법의 영향

  • No, Jun Hee (Department of Food and Nutrition, Chonnam National University) ;
  • Kim, Hyang Sook (Department of Food and Nutrition, Chungbuk National University) ;
  • Lee, Kyong Ae (Department of Food Science and Nutrition, Soonchunhyang University) ;
  • Shin, Malshick (Department of Food and Nutrition, Chonnam National University)
  • Published : 2012.10.31

Abstract

In comparison of the antioxidant activities by the different extraction methods of the domestic mung bean varieties, 'Geumsung', 'Dahyeon', 'Sohyeon', and 'Eohul', were soaked and dehulled, with the extracts obtained by using 80% ethanol at room temperature or heated. The mung bean hull was freeze-dried, ground, and passed through 100 mesh sieve. The moisture content of the hull powder ranged from 1.70 to 3.15%. The total dietary fiber content ranged from 84.42 to 88.47%, with the Sohyeon hull showing the highest value. The L value of Geumsung hull (62.93) was the highest, and the a value of Sohyeon hull (-1.69) was the lowest (p<0.05). The color difference was the highest in Dahyeon hull (42.52) and lowest in Geumsung hull (38.52). The Eohul hull's chlorophyll a and b contents were the highest with the 80% ethanol extract by heating (10.55 and 6.12 ${\mu}g/mL$, respectively), but the Sohyeon hull showed the highest in 80% ethanol extract at room temperature (7.63 and 3.93 ${\mu}g/mL$, respectively). The total phenolic and total flavonoid contents of the ethanol extracts were significantly different between the varieties regardless of the extraction temperature. The hull extract from the heating extraction was higher in Eohul than in other varieties, and was the lowest in the Dahyeon variety. The extract from Sohyeon hull at room temperature showed the highest contents of total phenolic and flavonoid contents. In the mung bean hull extract by heating, DPPH and ABTs radical scavenging activity ranged from 13.49-16.75% and 22.93-39.42% respectively and those at room temperature ranged from 39.86-41.88% and 49.71-70.92%. The reducing powers of extracts by heating ranged from 7.29 to 9.36 mg BHT/g hull, and at room temperature, they ranged from 19.90 to 20.97 mg BHT/g hull. Our results indicate that different extraction methods influenced antioxidant activities in the domestic mung bean varieties.

Keywords

References

  1. 신동화. 1995. 천연 항산화제의 연구와 전망. 식품기술. 8(2):28-36
  2. Amarowicz R, Estrella I, Hernandez T, Robredo S, Troszynska A, Kosinska A, Pegg RB. 2010. Free radical-scavenging capacity, antioxidant activity, and phenolic composition of green lentil (Lens culinaris). Food Chem 121:705-711 https://doi.org/10.1016/j.foodchem.2010.01.009
  3. Duh PD, Yen WJ, Du PC, Yen GC. 1997. Antioxidant activity of mung bean hulls. J Am Oil Chem Soc 74(9):1059-1063 https://doi.org/10.1007/s11746-997-0025-0
  4. Jeong SJ, Kang TH, Ko EB, Kim YC. 1998. Flavonoids from the seeds of Phasseolus radiatus. Korean J Pharmacogn 29(4):357-359
  5. Jin YI, Hong SU, Kim SJ, OK HC, Lee YJ, Nam JH, Yoon YH, Jeong JC, Lee SA. 2010. Comparison of antioxidant activity and amino acid components of mungbean cultivars grown in highland area in Korea. Korean J Environ Agric 29(4):381-387 https://doi.org/10.5338/KJEA.2010.29.4.381
  6. Kanatt SR, Arjun K, Sharma A. 2011. Antioxidant and antimicrobial activity of legume hulls. Food Res Int 44:3182-3187 https://doi.org/10.1016/j.foodres.2011.08.022
  7. Kim DK, Kim JB, Chon SU, Lee YS. 2005. Antioxidant potentials and quantification of flavonoids in mung bean (Virgna radiata L.) seeds. Korean J Plant Res 8(2):122-129
  8. Kim DK, Chon SU, Lee KD, Kim KH, Rim YS. 2008. Effect of seeding times on yield and flavonoid contents of mungbean. Korean J Crop Sci 53(3):273-278
  9. Kim DK, Son DM, Chon SU, Lee KD, Rim YS. 2009. Variation of vitexin and isovitexin contents in mungbean (Vigna radiata (L.) wilczek) germplasms. Korean J Plant Res 22(2):128-135
  10. Kim DK, Son DM, Choi JK, Chon SU. 2010. Growth property and seed quality of mungbean cultivars appropriate for labor saving cultivation. Korean J Crop Sci 55(3):239-244
  11. Koh KJ, Shin DB, Lee YC. 1997. Physicochemical properties of aqueous extracts in small red bean, mung bean and black soybean. Korean J Food Sci Technol 29(5):854-859
  12. Lai F, Wen Q, Li L, Wu H, Li X. 2010. Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment. Carbohy Polym 81:323-329 https://doi.org/10.1016/j.carbpol.2010.02.011
  13. Lee KJ, Yun IJ, Kim HY, Kim KH, Kim YJ, Kim DW, Lim SH. 2010. Antioxidative activity of solvent extracts from Synurus excelsus and Synurus palmatopinnatifidus. J Korean Soc Food Sci Nutr 39(12):1893-1897 https://doi.org/10.3746/jkfn.2010.39.12.1893
  14. Lee SC, Jeong SM, Kim SY. 2006. Effect of far-infrared radiation and heat treatment on the antioxidant activity of water extracts from peanut hulls. Food Chem 94:489-493 https://doi.org/10.1016/j.foodchem.2004.12.001
  15. Lee SH, Choi EO, Lee HG, Park KH. 2001. Factors affecting the components of chlorophyll pigment in spinach during storage. J Korean Soc Agric Biotechnol 44(2):73-80
  16. Li H, Cao D, Yi J, Cao J, Jiang W. 2012. Identification of the flavonoids in mungbean (Phaseolus radiatus L.) soup and their antioxidant activities. Food Chem 135:2942-2946 https://doi.org/10.1016/j.foodchem.2012.07.048
  17. Marathe SA, Rajalakshmi V, Sahayog N, Sharma JA. 2011. Comparative study on antioxidant activity of different varieties of commonly consumed legumes in India. Food Chem Toxicol 49:2005-2012 https://doi.org/10.1016/j.fct.2011.04.039
  18. Nam SH, Kang MY 2003. Screening of antioxidative activity of legume species. J Korean Soc Agric Chem Biotechnol 46(1):32-38
  19. Nithiyanantham S. Selvakumar S. Siddhuraju P. 2012. Total phenolic content and antioxidant activity of two different solvent extracts from raw and processed legumes, Cicer arietinum L. and Pisum sativum L.. J Food Composit Analy 27:52-60 https://doi.org/10.1016/j.jfca.2012.04.003
  20. Oh HS, Kim JH, Lee MH. 2003. Isoflavone contents, antioxidative and fibrinolytic activities of red bean and mung bean. Korean J Soc Food Cookery Sci 19(3):263-270
  21. Oh JH, Kim EH, Kim JL, Moon YI, Kang YH, Kang JS. 2004. Study on antioxidant potency of green tea by DPPH method. J Korean Soc Food Sci Nutr 33(7):1079-1084 https://doi.org/10.3746/jkfn.2004.33.7.1079
  22. Park SJ, Kim JM, Kim JE, Jeong SH, Park KH, Shin MS. 2011. Characteristics of sweet potato powders from eight Korean varieties. Korean J Food Cookery Sci 27(2):19-29 https://doi.org/10.9724/kfcs.2011.27.2.019
  23. Peng X, Zheng Z, Cheng KW, Shan F, Ren GX, Chen F, Wang M. 2008. Inhibitory effect of mung bean extract and its constituents vitexin and iosovitexin on the formation of advanced glycation endproducts. Food Chem 106:475-481 https://doi.org/10.1016/j.foodchem.2007.06.016
  24. Rajaei A, Barzegar M, Mobarez AM. 2010. Antioxidant, anti-microbial and antimutagenicity activities of pistachio (Pistachia vera) green hull extract. Food Chem Toxicol 48:107-112 https://doi.org/10.1016/j.fct.2009.09.023
  25. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying and improved ABTs radical cation decolorization assay. Free Radical Biol Med 26(9/10):1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  26. Siddhuraju P, 2006. The antioxidant activity and free radical-scavenging capacity of phenolics of raw and dry heated moth bean (Virgna aconitifolia) (jacq.) marechal seed extracts. Food Chem 99:149-157 https://doi.org/10.1016/j.foodchem.2005.07.029
  27. Wang SY, Wu JH, Ng TB, Ye XY, Rao PF. 2004. A non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. Peptides 25:1235-1242 https://doi.org/10.1016/j.peptides.2004.06.004
  28. Wellburn AR. 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307-313 https://doi.org/10.1016/S0176-1617(11)81192-2
  29. Zarco-Tejada PJ, Miller JR, Harron J, Hu B, Noland TL, Goel N, Mohammed GH, Sampson P. 2004. Needle chlorophyll content estimation through model inversion using hyperspectral data from boreal conifer forest canopies. Remote Sensing Environ 89:189-199 https://doi.org/10.1016/j.rse.2002.06.002

Cited by

  1. Anti-oxidant activities of mung bean starch and starch gels prepared from whole and hulled seeds vol.25, pp.2, 2016, https://doi.org/10.1007/s10068-016-0062-2
  2. Color Stability of Chlorophyll in Young Barley Leaf vol.26, pp.4, 2016, https://doi.org/10.17495/easdl.2016.8.26.4.314
  3. Color Stability of Traditional Fermented Rice Cake, Gijeongtteok Added with Chlorophyll-Stabilized Young Barley Leaf Powder vol.33, pp.5, 2012, https://doi.org/10.9724/kfcs.2017.33.5.504