• Journal of Life Science
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• v.29 no.8
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• pp.895-903
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• 2019

The aim of the present study was to improve the cell viability of human dental papilla derived single-induced pluripotent stem cells (iPSCs) using a Rho-associated protein kinase (ROCK) inhibitor, Y-27632. The iPSCs were produced using an episomal plasmid-based reprogramming method. After cell separation using trypsin, the iPSCs were treated with 0, 0.5, 1, 2.5, 5, 7.5, or $10{\mu}M$ Y-27632 for 5 d. Cell viability increased significantly following the $5{\mu}M$ Y-27632 treatment (p<0.05). When the iPSCs were exposed to medium containing $10{\mu}M$ Y-27632 for 0, 1, 2, 3, 4, and 5 d, the cell viability rate increased significantly in accordance with the cell viability rate (p<0.05). To evaluate the effect of the Y-27632 treatment on stemness characteristics, the expression of stem cell-specific transcripts and telomerase activity were investigated in the iPSCs treated with $10{\mu}M$ Y-27632 for 5 d. The expression levels of stem cell-specific transcripts, such as OCT-4, NONOG, and SOX-2, and telomerase activity were not significantly different in the iPSCs treated with $10{\mu}M$ Y-27632 as compared with those of untreated control iPSCs (p>0.05). Taken together, the results demonstrated that cell viability can be improved by treatment with the ROCK inhibitor Y-27632, without losing iPSC stemness characteristics.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.256-266
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• 2022

Empirical models including the Angstrom-Prescott (AP) model have been used to estimate solar radiation at sites, which would support a wide use of crop models. The objective of this study was to estimate two sets of solar radiation estimates using the AP coefficients derived for climate zone (AP_Frere) and specific site (AP_Choi), respectively. The daily solar radiation was estimated at 18 sites in Korea where long-term measurements of solar radiation were available. In the present study, daily solar radiation and sunshine duration were collected for the period from 2012 to 2021. Daily weather data including maximum and minimum temperatures and rainfall were also obtained to prepare input data to a process-based crop model, CERES-Rice model included in Decision Support System for Agrotechnology Transfer (DSSAT). It was found that the daily estimates of solar radiation using the climate zone specific coefficient, SFrere, had significantly less error than those using site-specific coefficients S_Choi (p<0.05). The cumulative values of S_Frere for the period from march to September also had less error at 55% of study sites than those of S_Choi. Still, the use of S_Frere and S_Choi as inputs to the CERES-Rice model resulted in slight differences between the outcomes of crop growth simulations, which had no significant difference between these outputs. These results suggested that the AP coefficients for the temperate climate zone would be preferable for the estimation of solar radiation. This merits further evaluation studies to compare the AP model with other sophisticated approaches such as models based on satellite data.

• Journal of Korea Water Resources Association
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• v.55 no.11
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• pp.931-939
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• 2022

In Korea, about two-thirds of the precipitation is concentrated in the summer season, so the problem of turbidity in the summer flood season varies from year to year. Concentrated rainfall due to abnormal rainfall and extreme weather is on the rise. The inflow of turbidity caused a sudden increase in turbidity in the water, causing a problem of turbidity in the dam reservoir. In particular, in Korea, where rivers and dam reservoirs are used for most of the annual average water consumption, if turbidity problems are prolonged, social and environmental problems such as agriculture, industry, and aquatic ecosystems in downstream areas will occur. In order to cope with such turbidity prediction, research on turbidity modeling is being actively conducted. Flow rate, water temperature, and SS data are required to model turbid water. To this end, the national measurement network measures turbidity by measuring SS in rivers and dam reservoirs, but there is a limitation in that the data resolution is low due to insufficient facilities. However, there is an unmeasured period depending on each dam and weather conditions. As a sensor for measuring turbidity, there are Optical Backscatter Sensor (OBS) and YSI, and a sensor for measuring SS uses equipment such as Laser In-Situ Scattering and Transmissometry (LISST). However, in the case of such a high-tech sensor, there is a limit due to the stability of the equipment. Therefore, there is an unmeasured period through analysis based on the acquired flow rate, water temperature, SS, and turbidity data, so it is necessary to develop a relational expression to calculate the SS used for the input data. In this study, the AEM3D model used in the Water Resources Corporation SURIAN system was used to improve the accuracy of prediction of turbidity through the turbidity-SS relationship developed based on the measurement data near the dam outlet.

• Journal of the Korean Chemical Society
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• v.34 no.5
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• pp.418-423
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• 1990

The protonation constants for the macrocyclic ligands 1,15,18-triaza-3,4;12,13-dibenzo-5,8,11-trioxa cycloeicosane (NdienOdienH$_4$), 1,12,15-triaza-3,4;9,10-dibenzo-5,8-dioxa cycloheptadecane (NdienOenH$_4$), and 1,15-diaza-3,4;12,13-dibenzo-5,8,11-trioxa cycloheptadecane (NenOdienH4) have been determined by the potentiometry in aqueous solutions (25$^{\circ}C$, I = 0.1, KNO$_3$). The stability constants for complexes formed in the aqueous solution (25$^{\circ}C$, I = 0.1, KNO$_3$) between the above ligands and the metal ions (Co(Ⅱ), Ni(Ⅱ), and Cu(Ⅱ)) have been measured by potentiometry. The rate of the ligand substitution reaction was measured spectrophotometrically by the addition of aqueous solutions of ethylenediamine to the solution of the complex. From the study of the temperature effect on the rate constant (k$_{obs}$), activation parameters (E$_a$,${\{Delta}H^{\neq}$, and ${\{Delta}S^{\neq}$) have been determined. The possible mechanism for the substitution reaction is proposed.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.5
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• pp.386-390
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• 2013

Recently, special attention has been given to acid rain and its problem to environment such as acid precipitation and air pollution in East Asia. In the present study, rainwater samples were collected from Apr to Nov in 2012. The samples were chemically characterized for the assessment of emission sources. Suwon and Yeoju regions, typical agricultural areas in South Korea, were chosen for study sites. Ion composition and cation-affected neutralization were determined to evaluate the contribution of cations to the acidity of rainwater. Ion and electrical conductivity between the measured and the estimated showed high correlation. The cations observed in Suwon and Yeoju were $Na^+$ > $NH_4{^+}$ > $K^+$ > $Ca^{2+}$ > $Mg^{2+}$ > $H^+$ and $Na^+$ > $K^+$ > $NH_4{^+}$ > $Ca^{2+}$ > $Mg^{2+}=H^+$, respectively. The anions of all sites were $SO{_4}^{2-}$ > $NO_3{^-}$ > $Cl^-$. While the amounts of sulfate, one of the major dissolved components of rainwater, were 77.6 and 75.6 ueq $L^{-1}$ in Suwon and Yeoju, the ones of NSS-$SO{_4}^{2-}$ (Non-Sea Salt sulfate) were 83 and 82% in Suwon and Yeoju, respectively. The comparison of observed pH values ($pH_{obs}$) with the theoretical pH values ($pH_{the}$) showed that the neutralization of rain water considerably went along during the study periods. The highest amounts of rainfall throughout the year in Suwon and Yeoju were 572.3 and 484.6 mm in July, and its corresponding nitrogen loadings in Suwon and Yeoju were 5.28 and 3.50 kg $ha^{-1}$, respectively. The major ion contents for crop growth with $SO{_4}^{2-}$, $Ca^{2+}$, $K^+$ and $Mg^{2+}$ were 51.7, 5.2, 11.8 and 1.8 kg $ha^{-1}$ in Suwon and 34.2, 4.0, 4.2 and 1.1 kg $ha^{-1}$ in Yeoju.

• Applied Biological Chemistry
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• v.40 no.1
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• pp.80-84
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• 1997

Series of new chiral N-substituted benzotriazol-1-yl derivatives were synthesized and their fungicidal activities in vitro against gray mold(Botrytis cinerea), black spot(Alternaria kikuchiana) and phytophthora blight(Phytophthora capsici) were measured by the agar medium dilution method. The substituents effects between the fungicidal activities (obs. $pI_{50}$) and a various physicochemical parameters of phenoxy or thiophenoxy group(X) & alkyl or phenyl group(Y) were analyzed by the multiple regression technique. From the analyzed substituent effects, the structure-activity relationship(SAR) equations shows that the antifungal activities depend on the parameters for the optimal molecular hydrophobicity($({\Sigma}logP)_{opt}$), Van der Waals (${\Sigma}Vw$>0) volume(${\AA}^3$) and inductive constant with electron withdrawing group(${\sigma}_I$,Y>0). The activity in affected by the inductive effect (${\sigma}_I$,Y>${\sigma}_g$X) of Y-group rather than the X-group. The phenoxy substituents, 1, showed higher antifungal activity tn the thiophenoxy substituents, 2. For 1, polar substituent constant(${\sigma}^*$) was an important factor in determining the activity. And the tribromomethyl substituent, 1g showed the highest activity against the tee fungi.

• Korean Journal of Environmental Agriculture
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• v.20 no.4
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• pp.284-288
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• 2001

This study was carried out to investigate the chemical properties of rainwater in Taean area. Rainwater was collected during seven months from April to October in 2000, and analyzed for its chemical composition. The pH of rainwater at April and May were higher than those from June to September. Occurrence rate of rain above pH 5.6 was 42.1%, which showed the highest ratio from rainwater samples during investigation periods. Those of pH $5.0{\sim}5.6$ and $4.5{\sim}5.0$ range were 21.1 and 31.6%, respectively. The major cation in rainwater were $Ca^{2+}$ and $NH_4\;^+$, and $SO_4\;^{2-}$ was more than 50% of total anion composition. Monthly variation of acidity neutralization capacity by $Ca^{2+}$ and $NH_4\;^+$ was decreased during rainy season. The $nss-SO_4\;^{2-}/NO_3\;^-$, ratio was 2.0 which means $SO_4\;^{2-}$ contributed to acidity of rainwater two times more than $NO_3\;^-$.

• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.733-740
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• 2008

The purpose of this study was to assess the genetic variation and establish the relationship amongst Hanwoo, Angus and Holstein breed. The genetic characteristics and variability within Hanwoo(300), Angus(80) and Holstein(50) were estimated on the basis of relationships determined using the 10 kinds of microsatellite, which is located on different chromosomes. Frequencies of microsatellites markers were used to estimate heterozygosities, polymorphic information content(PIC) and genetic distances. The PICs ranged from 0.604 to 0.872(Hanwoo), from 0.562 to 0.812(Angus) and from 0.471 to 0.828(Holstein). Observed heterozygosity and PIC of Hanwoo are the highest among the analyzed breeds. Additionally, Estimates of genetic distance can be utilized to identify genetic relationships between Hanwoo and the other breed. Genetic distances(0.233) between Hanwoo and Angus was lower than distances between Hanwoo and Holstein(2.283). Also, Genetic distances between Angus and Holstein was shown for(2,400). The other side, each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all the populations.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.1
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• pp.1-5
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• 2003

This study was carried out to investigate the chemical properties of rainwater in the Suwon area. Rainwater was collected from April to October in 1999 and 2000, and its chemical composition was analyzed. The pH of rainwater in April was higher than that of the months after June. Occurrence frequency of rain above pH 5.6 was 45.1%, which showed the highest ratio from rainwater samples during the investigation periods. Those of pH 5.0∼5.6 and 4.5∼4.9 range were 31.4 and 19.6%, respectively. The major cations in rainwater were $Ca^{2+}$ and N $H_{4}$$^{+}$, and S $O_{4}$$^{2-}$ made up more than 50% of total anion composition. Monthly variation of neutralization capacity of rainwater acidity by $Ca^{2+}$ and N $H_{4}$$^{+}$ decreased during the rainy season. The ratio of non-sea salt sulfate to nitrate (nss-S $O_{4}$$^{2-}$/N $O_{3}$$^{[-10]}$ ) was 2.1, which means anthropogenic S $O_{4}$$^{2-}$ contributed to acidity of rainwater two times more than N $O_{3}$$^{[-10]}$ . .

• Journal of Animal Science and Technology
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• v.58 no.11
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• pp.40.1-40.5
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• 2016

Background: Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people's weighted interest toward Jeju horses. Method: Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion: In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of $H_{obs}$ was 0.749 with a range from 0.612(HMS1) to 0. 857(ASB2). Also, it was found that $H_{\exp}$ and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of $H_{\exp}$ was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was $5.90{\times}10^{-20}$ when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were $4.08{\times}10^{-15}$ and $3.56{\times}10^{-8}$, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.