• Animal Bioscience
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• v.36 no.4
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• pp.654-670
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• 2023

Objective: This study aimed to develop loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) and compare it with LAMP-AGE, polymerase chain reaction (PCR), and standard Salmonella culture as reference methods for detecting Salmonella contamination in animal products and animal production environmental samples. Methods: The SalInvA01 primer, derived from the InvA gene and designed as a new probe for LFD detection, was used in developing this study. Adjusting for optimal conditions by temperature, time, and reagent concentration includes evaluating the specificity and limit of detection. The sampling of 120 animal product samples and 350 animal production environmental samples was determined by LAMP-LFD, comparing LAMP-AGE, PCR, and the culture method. Results: Salmonella was amplified using optimal conditions for the LAMP reaction and a DNA probe for LFD at 63℃ for 60 minutes. The specificity test revealed no cross-reactivity with other microorganisms. The limit of detection of LAMP-LFD in pure culture was 3×10² CFU/mL (6 CFU/reaction) and 9.01 pg/μL in genomic DNA. The limit of detection of the LAMP-LFD using artificially inoculated in minced chicken samples with 5 hours of pre-enrichment was 3.4×10⁴ CFU/mL (680 CFU/reaction). For 120 animal product samples, Salmonella was detected by the culture method, LAMP-LFD, LAMP-AGE, and PCR in 10/120 (8.3%). In three hundred fifty animal production environmental samples, Salmonella was detected in 91/350 (26%) by the culture method, equivalent to the detection rates of LAMP-LFD and LAMP-AGE, while PCR achieved 86/350 (24.6%). When comparing sensitivity, specificity, positive predictive value, and accuracy, LAMP-LFD showed the best results at 100%, 95.7%, 86.3%, and 96.6%, respectively. For Kappa index of LAMP-LFD, indicated nearly perfect agreement with culture method. Conclusion: The LAMP-LFD Salmonella detection, which used InvA gene, was highly specific, sensitive, and convenient for identifying Salmonella. Furthermore, this method could be used for Salmonella monitoring and primary screening in animal products and animal production environmental samples.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.40 no.1
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• pp.1-14
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• 2022

In order to conduct rapid, accurate and safe surveying at the excavation site, In this study, the possibility of underground facility survey using drones and the expected effect of 3D visualization were obtained as follows. Phantom4Pro 20MP drones have a 30m flight altitude and a redundant 85% flight plan, securing a GSD (Ground Sampling Distance) value of 0.85mm and 4points of GCP (Groud Control Point)and 2points of check point were calculated, and 7.3mm of ground control point and 11mm of check point were obtained. The importance of GCP was confirmed when measured with low-cost drones. If there is no ground reference point, the error range of X value is derived from -81.2 cm to +90.0 cm, and the error range of Y value is +6.8 cm to 155.9 cm. This study classifies point cloud data using the Pix4D program. I'm sorting underground facility data and road pavement data, and visualized 3D data of road and underground facilities of actual model through overlapping process. Overlaid point cloud data can be used to check the location and depth of the place you want through the Open Source program CloudCompare. This study will become a new paradigm of underground facility surveying.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.8 no.3
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• pp.467-475
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• 2018

Recently earthquakes have been increasing worldwide, and the largest earthquake of 5.8 on the Richter scale occurred on September 12, 2016 in the Gyeongju area. After the earthquake, more than 200 aftershocks have occurred from January 2017 to December 2017. The largest earthquake in 2017 was a 4.3-magnitude earthquake near Pohang on November 15. In this study, we tried to analyze the coordinate change due to the earthquake using the data of the CORS(Continuously Operating Reference Station) in Korea. In order to analyze the change of coordinates due to the earthquake in Pohang area on November 15, 2017, data processing was performed by kinematic method. And from January 2017 to December 2017, observation data of 9 stations in Korea were analyzed by relative positioning method and the change of coordinates due to earthquake was analyzed. As a result of the study, it was possible to estimate the instantaneous coordinate change due to the earthquake through the kinematic positioning, and it was suggested that there is no change in the coordinates of the domestic CORS by the relative positioning results. After the 2017 Gyeongju earthquake, aftershocks continue to occur, and it is necessary to monitor the area continuously.

• Journal of Korea Trade
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• v.27 no.2
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• pp.61-76
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• 2023

Purpose - The digital service trade has become an important driver of the global service trade. The main purpose of this study is to explore the influencing factors of digital service exports from China and Korea to RCEP sample countries respectively, and to comprehensively study the export potential of China and Korea to RCEP countries, so as to provide theoretical guidance and a decision-making reference to promote digital service trade exports and digital economy development in China and Korea. Design/methodology - First, the stochastic frontier gravity model was improved by introducing nonefficiency factors affecting digital services trade, extending the gravity model of traditional services trade exports to digital services trade exports. Secondly, the panel data of China and Korea for the eight sample countries of RCEP from 2011 to 2021 were adopted for the empirical analysis of digital service export potential by a stochastic frontier model. Findings - China's economic growth plays a role in increasing China's digital service trade exports, while Korea's economic growth does not play a significant role in increasing Korea's digital service trade exports. However, the economic growth of trading partner countries can play a significant role in boosting the digital service trade in both China and Korea, and comparison shows that Korea has higher resilience in the digital services trade than China. In addition, the market size of target countries plays a positive role in promoting the digital service trade exports of both China and Korea, and the increase in the value-added share of services in target countries will lead to a decrease in the digital service trade exports of both China and Korea. Originality/value - This study is innovative in terms of research perspective and method. Academic research on the export potential of international trade has been extensive, but most studies are based on the perspective of the goods trade, fewer studies are based on the perspective of the service trade, and there are almost no studies based on the perspective of digital service trade. There is a gap based on the comparative analysis of the export potential of the digital service trade between China and Korea. This study extends the gravitational model of traditional service trade exports to digital service trade exports to comparatively analyze the export potential of China and Korea to RCEP countries. This study addresses this limitation by analyzing a comparative analysis of the digital service trade export potential of China and Korea.

• Analytical Science and Technology
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• v.20 no.1
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• pp.33-40
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• 2007

This study centered on the quantitative analysis of about 27 trace elements including toxic ones using instrumental neutron activation analysis of fine ambient particulate matter in the third and fourth industrial complex area of Daejeon city, Korea. For analytical quality control, the certified reference material (NIST, the National Institute of Standards and Technology, U.S.A., SRM 2783, air particulate on filter media) was used. The errors relative to SRM values of Sb, Mn, V, Mg, Na, K, Ti, Co, Zn, and Sm fell below 5%, while those of Cr, Fe, Ba, Th, Ce, Al, and Cu were less than 10%. From the results of the quantitative analysis, the concentration of toxic metals such as As, Mn, Se, V, and Zn were $3.26{\pm}2.72$, $9.86{\pm}4.71$, $2.18{\pm}1.25$, $4.91{\pm}2.41$, $158{\pm}78ng/m^3$, respectively. And the results of factor analysis indicated that there are no more than six factors of sources of fine ambient particulate with statistical significance in the study area.

• Analytical Science and Technology
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• v.21 no.2
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• pp.93-101
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• 2008

The application of solid phase microextraction (SPME) is generally conducted by directly immersing the fiber into the liquid sample or by exposing the fiber in the head space (HS). The extraction temperature, the time of incubation, and application of stirring are often designated to be the most important parameters for achieving the best extraction efficiencies of HS-SPME analysis. In this study, relative importance of these three analytical parameters involved in the HS-SPME method is evaluated using a polydimethylsiloxane/carboxen (PDMS/CAR) fiber. To optimize its operation conditions the competing relationships between different parameters were investigated by comparing the extraction efficiency based on the combination of three parameters and two contracting conditions: (1) heating the sample at 30 vs. 50 C, (2) exposing samples at two durations of 10 vs. 30 min, and (3) application of stirring vs. no stirring. According to our analysis among 8 combination types of HS-SPME method, an extraction condition termed as S50-30 condition ((1) 1200 rpm stirring, (2) $50^{\circ}C$ exposure temp, and (3) 30 min exposure duration) showed maximum recovery rate of 45.5~68.5% relative to an arbitrary reference of direct GC injection. According to this study, the employment of stirring is the most crucial factor to improve extraction efficiency in the application of HS-SPME.

• Journal of the Korean Society of Food Culture
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• v.22 no.6
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• pp.765-774
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• 2007

Aging rate of rural area in our country is relatively high compared to that of the urban area. Thus, the introduction of meal service for the elderly residing in the rural area is necessary for their better living quality. Food habit, health and the nutritive intake conditions during the busy farming season were surveyed and comparative analysis of dietary intake for the introduction of meal service in pavilion of the elderly living in Chungnam, Kangwon, Jeonnam and Kyungbuk was performed for basic reference data of meal service introduction to the pavilion of the elderly in rural area. In general subject, the male elderly had a significant difference in marital state and showed that 79.4% was married and 20% was separated by death(P<0.05). In allowance, there were no significant difference but most of them lived with less then three hundred thousand won and especially, female lived with less then one hundred thousand won. In health state, the female elderly showed significant difference on difficulty with every day activity but with small trouble although they had to prepare their own meal(P<0.05). The condition dental health conditions of the female elderly had a significant difference showing bad conditions in following order; Kangwon(48%)>Chungnam(38.1%)>Kyungbuk(22.9%)>Jeonnam(22.5%)(p<0.05). The female elderly showed a significant difference in usage of denture and number of the female elderly without using the denture were very high(p<0.05). In nutrition intake condition, amount of sodium was very high but intakes of fiber and calcium were relatively 1ow(P<0.05). Meal service introduction in the pavilion of the elderly is suggested for the improvement of the life quality of the elderly in rural area. When developing the menu for them, conditions such as shortage of the fiber and calcium in diets, the dental conditions should be considered.

• Journal of Digestive Cancer Research
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• v.6 no.1
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• pp.25-31
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• 2018

Background: In Korea, stomach cancer is the second most common malignancy and the third leading cause of cancer-related deaths. the time of diagnosis is very important for treatment so early detection and surgery are currently considered the mainstay of treatment, when diagnosed advanced with tumor extension through the gastric wall and direct extension into other organs, with metastatic involvement. Recently, new drugs, drug combinations, and multimodal approaches have been used to treat this disease and In cancers over expressing or amplifying HER2, the combination of cisplatin-fluoropyrimidine-trastuzumab is considered to be the treatment of reference. but At present, the choice of treatment schedule for HER2-negative tumors is based on the medical institution's preferences and adverse effects profile. The aim of this study was to evaluate the effectiveness and safety of using FOLFOX regimen as a first-line therapy or a salvage therapy in the patients with HER2-negative advanced or metastatic gastric cancer. Methods: We retrospective reviewed the patient medical record from March 2012 to July 2017. This study evaluated 113 patients. Sixty-eight patients were treated with the FOLFOX regimen for the first time (first-line group) and 45 patients were treated with the FOLFOX regimen as a second (35 patients) or third (10 patients) chemotherapy (salvage group). Results: In the first-line group, the response rate was 54.9%. In the salvage therapy group, the response rate was 24.4% and The difference was statistically significant (p=0.205). The median TTP of the first-line group was 10.7 months (95% confidence interval [95% CI], 7.8-13.7 months) and that of salvage line group was 6.1 months (95% CI, 3.8-8.4 months). The median OS of the first-line group was 15.8 months (95% CI, 12.7-18.9 months) and that of the salvage therapy group was 10.2 months (95% CI, 8.2-11.9 months). drug toxicity was similar andtolerable between two groups. Conclusion: In patients with unresctable metastatic gastric cancer, after failing to respond to first-line therapy, most patients have no alternative other than second-line therapy because the disease is highly progressive. if the performance status of the patient is good enough to be eligible to treatments beyond best supportive care. FOLFOX regimen can be a considerable therapeutic option for salvage treatment.

• The Journal of Advanced Prosthodontics
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• v.15 no.5
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• pp.271-280
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• 2023

PURPOSE. This in vitro study aimed to compare the accuracy of the conventional facebow system and the newly developed POP (PNUD (Pusan National University Dental School) Occlusal Plane) bow system for occlusal plane transfer in asymmetric ear position. MATERIALS AND METHODS. Two dentists participated in this study, one was categorized as Experimenter 1 and the other as Experimenter 2 based on their clinical experience with the facebow (1F, 2F) and POP bow (1P, 2P) systems. The vertical height difference between the two ears of the phantom model was set to 3 mm. Experimenter 1 and Experimenter 2 performed the facebow and POP bow systems on the phantom model 10 times each, and the transfer accuracy was analyzed. The accuracy was evaluated by measuring the angle between the reference virtual plane (RVP) of the phantom model and the experimental virtual plane (EVP) of the upper mounting plate through digital superimposition. All data were statistically analyzed using a paired t-test (P < .05). RESULTS. Regardless of clinical experience, the POP bow system (0.53° ± 0.30 (1P) and 0.19° ± 0.18 (2P) for Experimenter 1 and 2, respectively) was significantly more accurate than the facebow system (1.88° ± 0.50 (1F) and 1.34° ± 0.25 (2F), respectively) in the frontal view (P < .05). In the sagittal view, no significant differences were found between the POP bow system (0.92° ± 0.50 (1P) and 0.73° ± 0.42 (2P) for Experimenter 1 and 2, respectively) and the facebow system (0.82° ± 0.49 (1F) and 0.60° ± 0.39 (2F), respectively), regardless of clinical experience (P > .05). CONCLUSION. In cases of asymmetric ear position, the POP bow system may transfer occlusal plane information more accurately than the facebow system in the frontal view, regardless of clinical experience.

• Korean Journal of Environmental Biology
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• v.41 no.3
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• pp.256-265
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• 2023

Chlorothalonil is continuously introduced into the marine environment and has significant toxic effects on various marine organisms, however, research on its effect on seaweed is limited. Therefore, we analyzed the impact of chlorothalonil on the early life stages of major aquaculture species in Korea, Undaria pinnatifida. U. pinnatifida female gametophytes were exposed to different concentrations of chlorothalonil (0, 0.03, 0.05, 0.10, 0.20, and 0.40 mg L^-1), and their survival rate and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 0.05, 0.10, and 0.141(0.121-0.166)mg L^-1. NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 0.10, 0.20, and 0.124 (0.119-0.131) mg L^-1. Therefore, female gametophytes of U. pinnatifida are expected to experience toxic effects at concentrations above 0.05-0.10 mg L^-1 of chlorothalonil. These research findings are anticipated to serve as crucial reference data for evaluating the effects of chlorothalonil on the health of U. pinnatifida in the early life stages.