Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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The impact of chlorothalonil on female gametophyte survival rate and relative growth rate of Undaria pinnatifida

Chlorothalonil이 미역(Undaria pinnatifida) 배우체의 생존 및 상대성장률에 미치는 영향

  • Yun-Ho Park (Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research) ;
  • Bo-Ram Sim (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Un-Ki Hwang (Tidal Flat Research Center, National Institute of Fisheries Science) ;
  • Ju-Wook Lee (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 박윤호 (국립환경과학원 환경건강연구부 위해성평가연구과) ;
  • 심보람 (국립수산과학원 서해수산연구소 기후환경자원과) ;
  • 황운기 (국립수산과학원 기후환경연구부 갯벌연구센터) ;
  • 이주욱 (국립수산과학원 서해수산연구소 기후환경자원과)
  • Received : 2023.08.03
  • Accepted : 2023.08.29
  • Published : 2023.09.30
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Abstract

Chlorothalonil is continuously introduced into the marine environment and has significant toxic effects on various marine organisms, however, research on its effect on seaweed is limited. Therefore, we analyzed the impact of chlorothalonil on the early life stages of major aquaculture species in Korea, Undaria pinnatifida. U. pinnatifida female gametophytes were exposed to different concentrations of chlorothalonil (0, 0.03, 0.05, 0.10, 0.20, and 0.40 mg L-1), and their survival rate and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 0.05, 0.10, and 0.141(0.121-0.166)mg L-1. NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 0.10, 0.20, and 0.124 (0.119-0.131) mg L-1. Therefore, female gametophytes of U. pinnatifida are expected to experience toxic effects at concentrations above 0.05-0.10 mg L-1 of chlorothalonil. These research findings are anticipated to serve as crucial reference data for evaluating the effects of chlorothalonil on the health of U. pinnatifida in the early life stages.

TBT 사용이 금지된 이후, 방오도료의 방오능력을 보완하기 위하여 booster biocides를 추가하여 사용하고 있다. Booster biocides 중에서 chlorothalonil은 해양환경 내 지속적으로 유입되며 다양한 해양생물에게 심각한 독성영향을 미치고 있지만 해조류에 대한 연구는 제한적이 다. 따라서 우리나라의 주요 양식생물인 미역(Undaria pinnatifida)의 초기생활사에 chlorothalonil이 미치는 영향을 분석하였다. U. pinnatifida의 암배우체를 chlorothalonil(0, 0.03, 0.05, 0.10, 0.20, 0.40 mg L-1)에 노출하여 생존율과 상대성장률을 분석하였다. 암배우체 생존율의 무영향 농도(NOEC), 최소영향농도(LOEC), 반수치사농도(LC50)는 0.05, 0.10, 0.141 (0.121~0.166) mg L-1이었고 상대성장률의 NOEC, LOEC, 반수영향농도(EC50)은 0.10, 0.20, 0.124 (0.119~0.131) mg L-1로 분석되었다. 따라서, 미역의 암배우체는 0.05~0.10 mg L-1 이상의 농도에서 독성영향을 받기 시작할 것으로 판단된다. 본 연구의 결과는 chlorothalonil에 대한 U. pinnatifida 초기생활사의 건강도를 평가하기 위한 기준자료로 활용될 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 2023년 국립수산과학원 경상과제(R2023009)의 연구비 지원으로 국립수산과학원 서해수산연구소 기후환경자원과에서 수행하였다

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