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Plant Regeneration of B.juncea Through Plant Tissue and Protoplast Culture

  • Lian, Yu-Ji;Lim, Hak-Tae
    • Journal of Plant Biotechnology
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    • v.3 no.1
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    • pp.27-31
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    • 2001
  • New types of cytoplasmic male sterility in Brassica species would be very useful for the production of F$_1$, hybrid seeds. Leaves and stems of rapid cycling stock of B.juncea (CrGC4-3) containing Anand CMS were used as experimental materials for plant regeneration from protoplast culture. Very high plant regeneration rate (85%) was found in the Kao & Michayluk medium supplemented with 2 mg/L zeatin, 0.5 mg/L BAP, and 1 mg/L NAA when only leaf, not stem, segments were cultured. Protoplasts were isolated from leaves using mixtures of enzymes (1% Cellulycin, 0.5% Macerozyme) in 0.4 M mannitol and 50 mM $CaCl_2$.$2H_2$O. Mcrocalli induced from protoplasts were transferred to the shoot regeneration medium containing 2 mg/L BAP, 2 mg/L zeatin, and 0.5 mg/L NAA. After 60 days of initial protoplast culture, regenerated plantlets were obtained, acclimatized, transplanted into the pots, and grown up to the flowering stage.

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New Non-Cascade Position Controller without Over-shoot Phenomena at Step Operal Condition of The Maximum Speed And The Maximum Current Restriction (최대 속도 및 최대 전류 제한 조건하에서 스텝 동작시의 오버-슛 현상을 억제시키는 새로운 비직렬형 위치제어기)

  • Lee, K.Y.;Kim, J.S.;Kang, S.J.;Kim, I.J.;Ahn, J.S.;Bae, S.H.
    • Proceedings of the KIEE Conference
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    • 2002.07b
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    • pp.1055-1057
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    • 2002
  • 산업이 발전할수록 정밀 위치제어 알고리즘에 대한 요구가 증가하고 있으며, 선형 전동기의 보급에 따라 1um 단위의 고급 위치 제어 알고리즘에 대한 중요성이 커지고 있다. 위치제어에 있어서 작업의 신속성 및 정밀도를 높이기 위해서는 제어의 오버슛 현상을 억제할 필요가 있으며, 현장에서 직접 활용이 가능하도록 하려면 보다 간단한 구조의 제어 알고리즘이 요구된다. 위치제어에서 가장 큰 문제는 전동기의 최대 속도 및 최대 전류 제한에 의한 비선형성이 위치의 과도한 오버슛으로 나타난다는 것이며, 기존의 고급 알고리즘에서는 미리 제공되는 S-커브 형태의 위치 명령 패턴을 사용하여 속도 및 전류 제한을 회피하고 있다. 본 연구에서는 고급 제어 알고리즘과 동일한 성능을 내면서 복잡한 패턴 생성기를 사용하지 않고도 실제 위치가 오버슛없이 부드러운 S-커브 형태로 목표값에 수렴하는 새로운 형태의 위치제어기를 제안한다. 제안된 제어기는 비교적 간단한 구조로 인하여 헌장 적용력이 매우 뛰어나며, 이득 설정이 간편하다는 장점을 지니고 있다.

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High-Efficiency Dual-Buck Inverter Using Coupled Inductor (결합 인덕터를 이용한 효율적인 단상 듀얼-벅 인버터)

  • Yang, Min-Kwon;Kim, Yu-Jin;Cho, Woo-Young
    • The Transactions of the Korean Institute of Power Electronics
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    • v.24 no.6
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    • pp.396-405
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    • 2019
  • Single-phase full-bridge inverters have shoot-through problems. Dead time is an essential way of solving these issues, but it distorts the output voltage and current. Dual-buck inverters are designed to eliminate the abovementioned problems. However, these inverters result in switching power loss and electromagnetic interference due to the diode reverse-recovery problem. Previous studies have suggested reducing the switching power loss from diode reverse-recovery, but their proposed methods have complex circuit configurations and high system costs. To alleviate the switching power loss from diode reverse-recovery, the current work proposes a dual-buck inverter with a coupled inductor. In the structure of the proposed inverter, the current flowing into the original diode is divided into a new diode. Therefore, the switching power loss is reduced, and the efficiency of the proposed inverter is improved. Simulation waveforms and experimental results for a 1.0 kW prototype inverter are discussed to verify the performance of the proposed inverter.

The Study of Expression Types and Aesthetic Meanings of Domestic Fashion Photographs Adopting Parody Storytelling (패러디 스토리텔링을 활용한 국내 패션화보의 유형 및 의미 연구)

  • Kwon, Gi Young
    • Human Ecology Research
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    • v.52 no.4
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    • pp.383-394
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    • 2014
  • The purpose of this study is to analyze the expression types and aesthetic meanings of fashion photographs adopting parody storytelling. The expressions of parody storytelling in advertising into the following five 5 types: parody of literature, movies, TV programs, art, and social issues. Therefore, in this study, we analyzed fashion photographs adopting parody storytelling from literature, movies, TV programs, art, and social issues, in fashion magazines. The meanings of fashion photographs adopting parody storytelling are as follows: First, the photographs adopting parody storytelling create new meanings with respect to a changeable narrative by transforming the original. Second, the humor of deviation comes from fun and interest generated the play of intent transformation and reconstruction of the original. Third, the photographs adopting parody storytelling of social issues suggest current messages. There is a growing tendency toward the value and the meanings of storytelling, and the various contents that have become the motive of the parody have important meanings. In particular, the analysis of expression methods and meanings of storytelling will contribute to raising the brand image through an effective fashion photographs project strategy. A story can be used as a source of inspiration and can contribute various ideas for a fashion photo-shoot and for fashion marketing.

Induced Resistance in Tomato Plants Against Fusarium Wilt Invoked by Nonpathogenic Fusarium, Chitosan and Bion

  • Amini, J.
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.256-262
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    • 2009
  • The potential of. nonpathogenic Fusarium oxysporum strain Avr5, either alone or in combination with chitosan and Bion, for inducing defense reaction in tomato plants inoculated with F. oxysporum f. sp lycopersici, was studied in vitro and glasshouse conditions. Application Bion at concentration of 5, 50, 100 and $500{\mu}g$/ml, and the highest concentration of chitosan reduced in vitro growth of the pathogen. Nonpathogenic F. oxysporum Avr5 reduced the disease severity of Fusarium wilt of tomato in split plants, significantly. Bion and chitosan applied on tomato seedlings at concentration $100{\mu}g$ a.i./plant; 15, 10 and 5 days before inoculation of pathogen. All treatments significantly reduced disease severity of Fusarium wilt of tomato relative to the infected control. The biggest disease reduction and increasing tomato growth belong to combination of nonpathogenic Fusarium and Bion. Growth rate of shoot and root markedly inhibited in tomato plants in response to tomato Fusarium wilt as compared with healthy control. These results suggest that reduction in disease incidence and promotion in growth parameters in tomato plants inoculated with nonpathogenic Fusarium and sprayed with elicitors could be related to the synergistic and cooperative effect between them, which lead to the induction and regulation of disease resistance. Combination of elicitors and non-pathogenic Fusarium synergistically inhibit the growth of pathogen and provide the first experimental support to the hypothesis that such synergy can contribute to enhanced fungal resistance in tomato. This chemical could provide a new approach for suppression of tomato Fusarium wilt, but its practical use needs further investigation.

Complex ESP Systems Proposal based on Pump Syringe and Electronically injector Modules for Medical Application

  • HAMROUNI, Chafaa
    • Journal of Multimedia Information System
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    • v.7 no.2
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    • pp.175-188
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    • 2020
  • The paper focuses on conception and development of complex systems composed mainly by a pump syringe subsystem and an electronically injector that facilitates patients saving data operation for medical staff use. We successfully developed conventional approaches for medical system staff requirements, such as system boundary conditions. Decisions at a given level are studied. We propose a complex system architecture, based mainly on patients collected data and ordered stepper injection parameters. System is successfully simulated and prototyped. Design and implement tests are accomplished, the proposed system ensures both the electric syringe pump and the electric injector operation. In addition, this new system introduces several additional options as patient database development and automation injection operation. Development and software operating tests to create a visualization control interface are validated. The solution performs syringe function and electronic injector. User can manage a syringe in two C modes of technology. We propose a program composed of two linked parts. If an error such radiologist bad target selection is made, an image with lower intrinsic quality emerges. Developed Shoot syringe different electronic cards are simulated and prototyped, in addition, maps are driven, prototype. All tests results are accomplished.

Tissue culture of the plant Pluchea indica (L.) Less. and evaluation of diuretic potential of its leaves

  • Pramanik, Kartick Chandra;Biswas, Ria;Mitra, Anupama;Bandyopadhyay, Durba;Mishra, Moumita;Chatterjee, Tapan Kumar
    • Advances in Traditional Medicine
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    • v.7 no.2
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    • pp.197-204
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    • 2007
  • The present study focused on the establishment of micropropagation protocol for the high value Pluchea (P.) indica (L.) Less., genotype, an important medicinal plant and evaluation of the diuretic activity of the leaf extract of the tissue cultured plant. Leaf explants, nodal segments and shoot tips were cultured in MS medium supplemented with auxin and cytokinin and their combinations. With the objective of inducing callus giving rise to new adult plants, naphthalene acetic acid was found to be most effective for (80%) for callus induction. The methanolic extract of leaves of the micropropagated P. indica was investigated for its diuretic activity in Wistar albino rats. Urinary excretion parameters were studied for evaluation of diuretic activity using Frusemide (20 mg/kg, p.o.) as standard. The extract showed significant diuretic activity at the doses of 100, 200 and 300 mg/kg. p.o. An oral acute toxicity study for the extract was carried out and the $LD_{50}$ value was found to be 2,825 mg/kg body weight.

Identification of Initiation Period and Subsequent Development of Floral Primordia in Black Locust (Robinia pseudoacacia L.)

  • Lee, Kyung Joon;Hong, Bongghi
    • Journal of Korean Society of Forest Science
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    • v.94 no.2 s.159
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    • pp.67-72
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    • 2005
  • The objectives of this study were to identify the period of initiation of floral primordia in black locust (Robinia pseudoacacia L.) and subsequent development of floral buds until following spring. Four mature trees of black locust located in Suwon, Korea were selected. Bud samples were collected from the current-year shoots, starting from mid June to July every week, from August to October and from February to April every month. The buds were fixed in FAA solution, dehydrated, and imbedded in paraffin for microscopic observation. Buds collected on June 16, and 23, 1997, contained primitive primordia that might be interpreted as early floral primordia. By June 30, a bud showed a positive indication of inflorescence primordium with a well-formed shoot apex. All the inflorescence primordia observed throughout the collection periods were always associated with unique hairy appendages around the primordium and enclosed within a sclerenchymatous chamber. By July 7 and 15, a floral apex had early bud scales. By July 22, primitive inflorescence developed into visible arrangement of individual floral primordial By July 29, the inflorescence developed into whirl arrangement of individual floral primordia in a transverse section, but showed little further development until October 15. The inflorescence primordia seemed to over-winter at this stage. Buds collected from February 15 and March 24 the following year also showed no further development of inflorescence primordia. By April 7 the inflorescence started to show further development with elongated axis. At this time individual flowers were easily recognized.

Improved Performance of SVPWM Inverter Based on Novel Dead Time and Voltage Drop Compensation (새로운 데드타임 및 전압강하의 보상을 이용한 SVPWM 인버터의 성능개선)

  • Lee, Dong-Hui;Gwon, Yeong-An
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.49 no.9
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    • pp.618-625
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    • 2000
  • Recently PWM inverters are widely utilized for many industrial applications e.g. high performance motor drive and PWM techniques are newly developed for an accurate output voltage. Among them space voltage vector PWM(SVPWM) inverter has high voltage ratio and low harmonics compared to the conventional sinusoidal PWM inverter. However output voltage of PWM inverter is distorted and has error duet o the conducting voltage drop of switching devices and the dead time that is inevitable to prevent the shoot-through phenomenon. This paper investigates 3-phase SVPWM inverter which has a new compensation method against dead time and voltage drop. Proposed algorithm calculates gate pulse periods which directly compensates the dead time and nonlinear voltage drop without modification of reference voltages. Direct compensation of gate pulse periods produces exact output voltage and does not need additional circuits. The propose algorithm is verified through the simulation and experiments.

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Heritabilities of Some Characters of Mulberry Trees (상수각 형질의 유전력)

  • 장권열;한경수;민병렬
    • Journal of Sericultural and Entomological Science
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    • v.10
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    • pp.41-43
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    • 1969
  • The experimental studies were intended to clarify the effects leaf yield calculations, and also aimed at estimating the heritabilities on some characters for the selection of desirable stocks of mulberry trees. The method of estimating heritabilities for the eight characters-branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, and leaf weight, was the variance components procedures in a replicated trial for the varietal lines. The results are summarized as follows: Means, variances and standard errors for the characters are shown in table 1, and the results of variance analyses are also shown in table 2. Heritability values of node number was the highest, and these of branch number per stock was the lowest, and these of other six characters were intermediate values. These all calculated heritability values were higher than anticipated. This was expected since these values, which were the broad sense heritability, contain the variance due to dominance and epistasis in addition to the additive variance.

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