The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Induced Resistance in Tomato Plants Against Fusarium Wilt Invoked by Nonpathogenic Fusarium, Chitosan and Bion

  • Amini, J. (Department of Plant Protection, University of Kurdistan)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The potential of. nonpathogenic Fusarium oxysporum strain Avr5, either alone or in combination with chitosan and Bion, for inducing defense reaction in tomato plants inoculated with F. oxysporum f. sp lycopersici, was studied in vitro and glasshouse conditions. Application Bion at concentration of 5, 50, 100 and $500{\mu}g$/ml, and the highest concentration of chitosan reduced in vitro growth of the pathogen. Nonpathogenic F. oxysporum Avr5 reduced the disease severity of Fusarium wilt of tomato in split plants, significantly. Bion and chitosan applied on tomato seedlings at concentration $100{\mu}g$ a.i./plant; 15, 10 and 5 days before inoculation of pathogen. All treatments significantly reduced disease severity of Fusarium wilt of tomato relative to the infected control. The biggest disease reduction and increasing tomato growth belong to combination of nonpathogenic Fusarium and Bion. Growth rate of shoot and root markedly inhibited in tomato plants in response to tomato Fusarium wilt as compared with healthy control. These results suggest that reduction in disease incidence and promotion in growth parameters in tomato plants inoculated with nonpathogenic Fusarium and sprayed with elicitors could be related to the synergistic and cooperative effect between them, which lead to the induction and regulation of disease resistance. Combination of elicitors and non-pathogenic Fusarium synergistically inhibit the growth of pathogen and provide the first experimental support to the hypothesis that such synergy can contribute to enhanced fungal resistance in tomato. This chemical could provide a new approach for suppression of tomato Fusarium wilt, but its practical use needs further investigation.

Keywords

References

  1. Aducci, P., Ballio, A. and Marra, M. 1997. Phytotoxins as molecular signals. In: Signal transduction in plants. ed. by P. Aducci, pp. 83-105. Birkhauser Verlag, Basel, Switzerland
  2. Anith, K. N., Momol, M. T., Kloepper, J. W., Marios, J. J. and Olson, S. M. 2004. Efficacy of plant growth-promoting rhizobacteria, acibenzolar-S-methyl, and soil amendment for integrated management of bacterial wilt on tomato. Plant Dis. 88:669-673 https://doi.org/10.1094/PDIS.2004.88.6.669
  3. Bautista, B. S., Hernandez, L. A. N., Velazquez, D. V. and Hernandez, L. M. 2006. Chitosan as a potential natural compound to control pre and post harvest diseases of horticultural commodities. Crop Prot. 25:108-118 https://doi.org/10.1016/j.cropro.2005.03.010
  4. Benhamou, N., Lafontaine, P. J. and Nicole, M. 1994. Induction of systemic resistance to Fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology 84:1432-1444 https://doi.org/10.1094/Phyto-84-1432
  5. Benhamou, N., Kloepper, J. W. and Tuzun, S. 1998. Induction of resistance against Fusarium wilt of tomato by combination of chitosan with an endophytic bacterial strain: ultrastructure and cytochemistry of the host response. Planta. 204:153-168 https://doi.org/10.1007/s004250050242
  6. Bora, T., Ozaktan, H., Gore, E. and AsIan, E. 2004. Biological control of Fusarium oxysporum f. sp. Melonis by wettable powder formulation of the two strain of Pseudomonas putida. J. Phytopathol. 152:471-475 https://doi.org/10.1111/j.1439-0434.2004.00877.x
  7. Bubici, G., Amenduni, M., Colella, C. and Cirulli, M. 2006. Efficacy of acibenzolar-S-methyl and two strobilurins, azoxystrobin and trifloxystrobin, for the control of corky root of tomato and Verticillium wilt of eggplant. Crop Prot. 25:814-820 https://doi.org/10.1016/j.cropro.2005.06.008
  8. Dandurand, L. M. and Knudsen, G. R. 1993. Influence of Pseudomonas fluorescens on hyphal growyh and biocontrol activity of Trichoderma harzianum in the spermosphere and rhizosphere of pea. Phytopathology 82:265-270
  9. Davis, D., Merida, J., Legendre, L., Low, P. S. and Heinstein, P. 1993. Independent elicitation of the oxidative brust and phytoalexin formation in cultured plant cells. Phytochemistry 32:607-611 https://doi.org/10.1016/S0031-9422(00)95144-6
  10. Duffy, B. K. and Weller, D. M. 1995. Use of Gaeumannomyces graminis var. graminis alone and in combination with fluorescent Pseudomonads to suppress take-all of wheat. Plant Dis. 79:907-911 https://doi.org/10.1094/PD-79-0907
  11. Duijff Ben, J., Pouhair, D., Plivain, C., Alabouvette, C. and Lemanceau, P. 1998. Implication of systemic induced resistance in the suppression of Fusarium wilt of tomato by Pseudomonas jluorescens WCS417r and nonpathogenic Fusarium oxysporum Fo47. Eur. J. Plant Pathol. 104:903-910 https://doi.org/10.1023/A:1008626212305
  12. Eikemo, H., Stensvand, A. and Tronsmo, A. M. 2003. Induced resistance as a possible means to control diseases of strawberry caused by Phytophthora spp. Plant Dis. 87:345-350 https://doi.org/10.1094/PDIS.2003.87.4.345
  13. Fuchs, J. G., Moenne, L. and Defago, Y. 1997. Nonpathogenic Fusarium oxysporum strain Fo47 induces resistance to Fusarium wilt in tomato. Plant Dis. 81:492-496 https://doi.org/10.1094/PDIS.1997.81.5.492
  14. Gorlach, J., Volrath, S., Kanauf-beiter, G. and Hengy, G. 1996. Benzothiadiazole, a nevel class of inducers of systemic acquired resistance activates gene expression and disease reduction in wheat. Plant Cell 8:629-643 https://doi.org/10.1105/tpc.8.4.629
  15. Hoffland, E., Hakulinen, J. and Van Pelt, J. A. 1996. Comparison of systemic resistance induced by avirulent and non-pathogenic Pseudomonas species. Phytopathology 86:757-762 https://doi.org/10.1094/Phyto-86-757
  16. Hofgaard, S., Ergon, A., Wanner, L. A. and Tronsmo, A. M. 2005. The Effect of chitosan and Bion on resistance to pink snow mould in perennial ryegrass and winter wheat. J. Phytopathol. 153:108-119 https://doi.org/10.1111/j.1439-0434.2005.00937.x
  17. Inbar, M., Doostdar, H., Sonoda, R. M., Leibee, G. L. and Mayer, R. T. 1998. Elicitors of plant defensive systems reduce insect densities and disease incidence. J. Chem. Ecol. 24:135-149 https://doi.org/10.1023/A:1022397130895
  18. Jones, J. B., Jones, J. P., Stall, R. E. and Zitter, T. A. 1991. Compendium of tomato diseases. The American Phytopathological Society St. Paul. MN
  19. Kamal, A. M., Abo-Elyousr, H. H. and Hendawy, E. 2008. Integration of Pseudomonas fluorescens and acibenzolar-S-methyl to control bacterial spot disease of tomato. Crop Prot. 27:1118-1124 https://doi.org/10.1016/j.cropro.2008.01.011
  20. Lemanceau, P. and Alabouvette, C. 1991. Biological control of Fusarium diseases by fluorescent Pseudomonads and nonpathogenic Fusarium. Crop Prot. 10:279-286 https://doi.org/10.1016/0261-2194(91)90006-D
  21. Louws, F. J., Wilson, M., Campbell, H. L., Cuppels, D. A., Jones, J. B. and Shoemaker, P. B. 2001. Field control of bacterial spot and bacterial speck of tomato using a plant activator. Plant Dis. 85:481-488 https://doi.org/10.1094/PDIS.2001.85.5.481
  22. Mes, J. J., Weststeiji, E. A., Herlar, F. and Lambalk, J. J. 1999. Biological and molecular characterization of Fusarium oxysporum f. sp. lycopersici divides race 1 isolates into separate virulence groups. Phytopathology 89:156-160 https://doi.org/10.1094/PHYTO.1999.89.2.156
  23. Oostenorp, M., Kunz, W., Dietrich, B. and Staub, T. 2001. Induced disease resistance in plant by chemicals. Eur. J. Plant. Pathol. 107:19-28 https://doi.org/10.1023/A:1008760518772
  24. Reddy, M. B., Belkacemi, K., Corcuff, R., Castaigne, F. and AruI, J. 2000. Effect of preharvest chitosan sprays on post-harvest infection by Botrytis cinerea and quality of strawberry fruit. Postharvest Biol. Biotechnol. 20:39-51 https://doi.org/10.1016/S0925-5214(00)00108-3
  25. Samia, M. and Khallal, E. L. 2007. Induction and Modulation of resistance in tomato plants against Fusarium wilt disease by Bioagent fungi (Arbuscular Mycorrhiza) and/or hormonal elicitors (Jasmonic acid & Salicylic acid). Aust. J. Basic Appl. Sci. 1:691-705
  26. Sticher, L., Mauchmani, B. and Metraux, J. P. 1997. Systemic acquired resistance. Annu. Rev. Phytopathol. 35:235-270 https://doi.org/10.1146/annurev.phyto.35.1.235
  27. Tiuterev, S., Yakubchik, M., Tarlakovsky, S., Popova, E., Vytsky, V. and Dorofeyeva, T. 1996. Chitosan: mechanism of action and ways of using as ecologically safe means in enhancement of plant disease resistance. Arch. Phytopathol. Plant Prot. 30:323-332 https://doi.org/10.1080/03235409609383183
  28. Uknes, S. J., Mauch-Mani, B., Moyer, M., Potter, S., Williams, S., Dincher, S. S. and Chandler, D. 1992. Acquired resistance in Arabidopsis. Plant Cell 4:645-656 https://doi.org/10.1105/tpc.4.6.645
  29. Wade, H. and Elmer, W. H. 2006. Effects of acibenzolar- S. methyl on the suppression of Fusarium wilt of cyclamen. Crop Prot. 25:671-676 https://doi.org/10.1016/j.cropro.2005.09.010
  30. Yoo, S. Y. and Lee, S. K. 1999. Effect of various films on shelflife of sliced strawberry fruit. Acta Hortic. 483:283-289

Cited by

  1. Activation of tomato plant defence responses against bacterial wilt caused by Ralstonia solanacearum using DL-3-aminobutyric acid (BABA) vol.136, pp.1, 2013, https://doi.org/10.1007/s10658-012-0149-4
  2. Induction of lignin and pathogenesis related proteins in dragon fruit plants in response to submicron chitosan dispersions vol.63, 2014, https://doi.org/10.1016/j.cropro.2014.05.009
  3. Regulation of inducible enzymes and suppression of anthracnose using submicron chitosan dispersions vol.193, 2015, https://doi.org/10.1016/j.scienta.2015.07.014
  4. The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro vol.57, pp.1, 2013, https://doi.org/10.1007/s10535-012-0254-4
  5. Ultrastructural Study on Induced Resistance of Cucumber Plants against Sphaerotheca fuliginea by Oligochitosan vol.27, pp.1, 2011, https://doi.org/10.5423/PPJ.2011.27.1.008
  6. Biochemical basis of improvement of defense in tomato plant against Fusarium wilt by CaCl2 vol.23, pp.3, 2017, https://doi.org/10.1007/s12298-017-0450-y
  7. The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage vol.50, pp.4, 2012, https://doi.org/10.1007/s12275-012-2105-6