Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Tissue culture of the plant Pluchea indica (L.) Less. and evaluation of diuretic potential of its leaves

  • Published : 2007.06.30
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Abstract

The present study focused on the establishment of micropropagation protocol for the high value Pluchea (P.) indica (L.) Less., genotype, an important medicinal plant and evaluation of the diuretic activity of the leaf extract of the tissue cultured plant. Leaf explants, nodal segments and shoot tips were cultured in MS medium supplemented with auxin and cytokinin and their combinations. With the objective of inducing callus giving rise to new adult plants, naphthalene acetic acid was found to be most effective for (80%) for callus induction. The methanolic extract of leaves of the micropropagated P. indica was investigated for its diuretic activity in Wistar albino rats. Urinary excretion parameters were studied for evaluation of diuretic activity using Frusemide (20 mg/kg, p.o.) as standard. The extract showed significant diuretic activity at the doses of 100, 200 and 300 mg/kg. p.o. An oral acute toxicity study for the extract was carried out and the $LD_{50}$ value was found to be 2,825 mg/kg body weight.

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References

  1. Anonymous. (2000) Gereral guidelines for methodologies on research and evaluation of traditional medicine, World Health Organization, Geneva
  2. Bhattacharya R, Bhattacharya S, Wenzel-Mathers M, Buckwold VE. (2003) Phyllanthus amarus root clone with significant activity against bovine viral diarrhea virus - a surrogate model of hepatitis C virus. Curr. Sci. 84, 529
  3. Biswas R, Dasgupta A, Mitra A, Roy SK, Dutta PK, Achari B, Dastidar SG, Chatterjee TK. (2005) Isolation, purification and characterization of four pure compounds from the root extract of Pluchea indica (L.) Less. and the potentiality of the root extract and the pure compounds for antimicrobial activity. Eur. Bull. Drug Res. 13, 63-70
  4. Chakravarty AK, Mukhopadhyay S. (1994) New thiophene derivatives from Pluchea indica. Indian J. Chem. 33, 978-980
  5. Chatterjee TK. (1996) Herbal Options, M/s Eastern Traders, Calcutta, India, 183, 215
  6. Ghosh MN. (1994) Fundamentals of Experimental Pharmacology. Scientific Book Agency, Kolkata, India, 153-158
  7. Kirtikar KR, Basu BD. (1999) Indian Medicinal Plants. International Book Distributors, Dehradun, India, 1344-1345
  8. Kukreja AK, Mathur AK. (1985) Tissue Culture studies in Duboisia myoparoides I - Plant regeneration and clonal propagation by stem node cultures. Planta Med. 2, 93 https://doi.org/10.1055/s-2007-969415
  9. Kukreja AK, Mathur AK, Abuja PS. (1986) Morphogenetic potential of foliar explants in Duboisia myoporoides R. Br. (Solanaceae). Plant Cell Rep. 5, 27 https://doi.org/10.1007/BF00269711
  10. Lipschitz WL, Hadidian Z, Kerpcar A. (1943) Bioassay of Diuretics. J. Pharmacol. Exp. Ther. 79, 97-110
  11. Luanratana O. (1997) Micrapopagation of Duboisia spp., In: Biotechnology in Agriculture and Forestry, edited by YPS Bajaj, p. 313, Springer - Verlag Berline Heidelberg New York
  12. Mathur J, Ahuja PS, Mathur A, Kukreja AK, Shah NC. (1988) In vitro propagation of Valeriana wallichi. Planta Med. 54, 82 https://doi.org/10.1055/s-2006-962346
  13. Motooka P, Castro L, Nelson D, Nagai G, Ching L. (2002) Weeds of pastures and natural areas of Hawaii and their management
  14. Mukhopadhyay S, Cordell GA, Ruangrungsi N, Rodkird S, Tantivatana P, Hylands PJ. (1983) Traditional medicinal plants of Thailand. IV. 3-(2',3'-Diacetoxy-2'-methyl butyryl)-cuauhtemone from Pluchea indica. J. Nat. Prod. 46, 671-674
  15. Murashige T, Skoog F. (1962) A revised medium for rapid growth and bio-assay with Tobacco tissue culture. Physiol. Plantarum 15, 473 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Na H, Kondo K. (1986) Plant Tissue culture Lett. 12, p. 273, Conservation of genes-resources in epiphytic Vanda pumila (Orchidaceae) by tissue cultured shoot primordial
  17. Peungvicha P, Temsiririrkul R, Prasain JK, Tezuka Y, Kadota S, Thirawarapau SS, Watanabe H. (1999) The hypoglycaemic effect of Pluchea indica Less root in normal and diabetic rats. Thai J. Phytopharm. 6, 18-22
  18. Ramesh R, Anbu M. (1996) Chemical methods for environmental analysis: water and sediment Macmillan India Publishers, Madras, India, p. 161
  19. Sato K, Tanaka R, Taniguchi K, Miagawa H, Okada M. (1987) Clonal mass propagation of orchids by means of tissue culture shoot primordial. In: Proceedings of world orchid Hiroshima symposium. edited by K Kondo and Hasimoto, pp. 129, Hiroshima
  20. Sen T, Dhara AK, Bhattacharjee S, Pal S, Nag Chaudhuri AK. (2002) Antioxidant activity of the methanol fraction of Pluchea indica root extract. Phytother. Res. 16, 331-335 https://doi.org/10.1002/ptr.892
  21. Sen T, Ghosh TK, Chaudhuri AK. (1993) Studies on the mechanism of anti-inflammatory and anti-ulcer activity of Pluchea indica-probable involvement of 5-lipooxygenase pathway. Life Sci. 52, 737-743 https://doi.org/10.1016/0024-3205(93)90236-V
  22. Sen T, Nag Chaudhuri AK. (1991) Antiinflammatory evaluation of a Pluchea indica root extract. J. Ethnopharmacol. 33, 135-141 https://doi.org/10.1016/0378-8741(91)90172-A
  23. Tanaka R, Ikeda H. (1983) Perennial maintenance of annual Haploppapus gracilis (2n=4) by shoot tip cloning. Jpn. J. Genet. 58, 65
  24. Thongpraditchote S, Matsumoto K, Temsiririrkkul R, Tohda M, Murakami Y, Watanabe H. (1996) Neuropharmacological actions of Pluchea indica Less. root extract in socially isolated mice. Biol. Pharm. Bull. 19, 379-383
  25. Tideman J, Hawker JS. (1982) In vitro propagation of latex producing plants. Ann. Bot. 49, 273
  26. Uchiyama T, Miyase T, Uena A, Khan U. (1989) Terpenic glycosides from Pluchea indica. Phytochemistry 28, 3369-3372.
  27. Uchiyama T, Miyase T, Ueno A, Khan U. (1991) Terpene and Lignan glycosides from Pluchea indica. Phytochemistry 30, 655-657 https://doi.org/10.1016/0031-9422(91)83746-8