• 한국임상수의학회지
• /
• 제17권1호
• /
• pp.13-20
• /
• 2000

Recently cases of canine distemper have occurred in Korea despite vaccination was carried out nationwidely. This study was performed to establish rapid diagnosis of canine distemper by RT-PCR, nested PCR, and serological test. A total of 30 dogs, which were suspected canine distemper clinically, was examined. RT-PCR and nested PCR were specific for the amplification of CDV H gene and sensitive to detect 7 TCID50 of Onderstepoort strain. By RT-PCR, H gene was detected in 6(20%) of 30 peripheral bloods from dogs. And H gene was detected in 10(33.3%) of 30 samples by nested PCR. H gene was detected from 1 brain of 6 years-old Beagle dog and 1 lung of 2 months-old Shihtzu dog, in which peripheral blood H gene was not detected. Serum neutralizing antibody titer against Onderstepoort strain ranged from 4 to 1,024 in 30 patients. No correlation was observed between the results of nested PCR and titiers of neutralizing antibody.

• 대한임베디드공학회논문지
• /
• 제12권3호
• /
• pp.159-167
• /
• 2017

Software safety is a key issue in embedded system of automotive and aviation industries. Various software testing approaches have been proposed to achieve software safety like ISO26262 Part 6 in automotive environment. In spite of one of the classic and basic approaches, stack memory is hard to estimating exactly because of uncertainty of target code generated by compiler and complex nested interrupt. In this paper, we propose an approach of analyzing the maximum stack usage statically from target binary code rather than the source code that also allows nested interrupts for determining the exact stack memory size. In our approach, determining maximum stack usage is divided into three steps: data extraction from ELF file, construction of call graph, and consideration of nested interrupt configurations for determining required stack size from the ISR (Interrupt Service Routine). Experimental results of the estimation of the maximum stack usage shows proposed approach is helpful for optimizing stack memory size and checking the stability of the program in the embedded system that especially supports nested interrupts.

• Journal of the Korean Data and Information Science Society
• /
• 제24권6호
• /
• pp.1439-1448
• /
• 2013

확률화 블록계획법이나 교차된 이원분류표에 대한 분산분석과 오차제곱합의 성질은 널리 알려져있다. 본 논문에서는 인자에 따른 수준에 또 다른 인자들이 내포되어 있는 지분계획 특히 이단계 지분계획에 대하여 구조적 특징을 설명하고 오차제곱합의 성질에 대하여 살펴보았다. 또한 이단계 지분계획의 활용으로 크로스오버 계획을 소개하고 생물학이나 약학 등의 분야에서 많이 사용되는 동등성 검정의 신뢰구간 구축에 대하여 설명하였고 실제자료와 SPSS 통계 페키지를 이용하여 분석함으로서 응용성을 부각시켰다.

• 대한수의학회지
• /
• 제60권3호
• /
• pp.109-116
• /
• 2020

This study aimed to develop a semi-nested polymerase chain reaction assay for the direct detection of Mycoplasma synoviae (M. synoviae) from clinical samples using three newly designed oligonucleotide primers specific to the variable lipoprotein haemagglutinin (vlhA) gene and differentiate M. synoviae field strains based on a nucleotide deletion or the insertion of the proline-rich repeat (PRR) region of the vlhA gene. The developed semi-nested polymerase chain reaction (PCR) assay revealed positive results in 12 out of 100 clinical samples collected from chickens showing lameness and joint swelling. Six positive samples were selected randomly for sequencing, and sequence analysis revealed 96.3-100% nucleotide identities compared to the reference sequences. Phylogenetic analysis showed that sequences of the strains in this study were closely related to WVU1853 (Spain), CK.MS.UDL.PK.2014.2 (Pakistan), and F10-2AS (USA) strains, but they were distinct from the M. synoviae-H vaccine strain sequence. M. synoviae obtained from these samples were identified as types A and C with a length of 38 and 32 amino acids, respectively. These results indicated that the specific and sensitive semi-nested PCR could be a useful diagnostic tool for the direct identification of clinical samples, and the sequence analysis of the partial vlhA gene can be useful for typing M. Synoviae.

• Archives of Pharmacal Research
• /
• 제19권6호
• /
• pp.469-474
• /
• 1996

A rapid and sensitive method has been developed to detect hepatitis B virus DNA (HBV) by nested polymerase chain reaction (PCR) technique with primers specific for the surface and core regions in capillary thermal cycler within 80 min. The lower limit for detection by present PCR method is $10^{-5}$ pg of recombinant HBV DNA which is equivalent to that determined by one round of PCR amplification and Southern blot hybridization analysis. When boiled HBV positive serum was serially diluted 10-fold, HBV DNA was successfully determined in $1{\mu}l-10^{-3}$ of serum. HBV DNA was detected by present method in 69 clinical samples including HBsAg positives and negatives by enzyme-linked immunosorbent assay (ELISA). When serum samples were amplified by nested PCR using surface and core region primers, HBV DNAs were detected in 37 of 69 samples (53.6%) and 18 of 69 samples (26.1%), respectively. These results can inform the infectious state of HBsAg positive pateints. A simple and rapid nested PCR protocol by using boiled serum as DNA template has been described for the clinical utility to determine HBV DNA in human serum.

• Journal of Microbiology
• /
• 제38권4호
• /
• pp.260-264
• /
• 2000

Reverse transcription (RT)-PCR and nested PCR methods (2-step PCR) were tested for their ability to detect marine birnavirus (MBV) in cultured rockfish, Sebastes schlegeli. One set of primers for RT-PCR was designed, based on a gene of infectious pancreatic necrosis virus (IPNV), and another set of primers for nested PCR was designed based on the VP2/NS junction region of MBV. This 2-step PCR method was specific for MBV and sensitivity was heightened when nested PCR was combined to RT-PCR. This 2-step PCR method was useful for detecting MBV not only in diseased fish, but also in asymptomatic fish. These results indicate that this 2-step PCR method is useful for detecting MBV in rockfish.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제13권5호
• /
• pp.2454-2468
• /
• 2019

The existing direct position determinations(DPD) for coherently distributed(CD) sources are mostly applicable for uniform linear array(ULA), which result in a low degree of freedom(DOF), and it is difficult for them to realize the effective positioning in underdetermined condition. In this paper, a novel DPD algorithm for coherently distributed sources based on compressed sensing with a moving nested array is present. In this algorithm, the nested array is introduced to DPD firstly, and a positioning model of signal moving station based on nested array is constructed. Owing to the features of coherently distributed sources, the cost function of compressed sensing is established based on vectorization. For the sake of convenience, unconstrained transformation and convex transformation of cost functions are carried out. Finally, the position coordinates of the distribution source signals are obtained according to the theory of optimization. At the same time, the complexity is analyzed, and the simulation results show that, in comparison with two-step positioning algorithms and subspace-based algorithms, the proposed algorithm effectively solves the positioning problem in underdetermined condition with the same physical element number.

• 대한의생명과학회지
• /
• 제24권4호
• /
• pp.390-397
• /
• 2018

For the specific detection of human Parvovirus B19 (HuPaV-B19), we designed ten specific PCR primers from 3,800~4,500 nucleotides of HuPaV-B19 complete genome (NC_000883.2). Seventeen candidate PCR primer sets for specific detecting HuPaV-B19 were constructed. In specific reaction of HuPaV-B19, seventeen PCR primer sets showed specific band, however five PCR primer sets were selected basis of band intensity, amplicon size and location. In non-specific reaction with seven reference viruses, four PCR primer sets showed non-specific band, however one PCR primer set is not. Detection sensitivity of final selective PCR primer set was $100fg/{\mu}L$ for 112 minute, and PCR amplicon is 539 base pairs (bp). In addition, nested PCR primer set was developed, for detection HuPaV-B19 from a low concentration of contaminated samples. Selection of nested PCR primer set was basis of sensitivity and groundwater sample tests. Detection sensitivity of final selective PCR and nested PCR primer sets for the detection of HuPaV-B19 were $100fg/{\mu}L$ and $100ag/{\mu}L$ basis of HuPaV-B19 plasmid, it was able to rapid and highly sensitive detection of HuPaV-B19 than previous reports. We expect developed PCR primer set in this study will used for detection of HuPaV-B19 in various samples.

• 응용통계연구
• /
• 제14권1호
• /
• pp.103-110
• /
• 2001

본 논문은 다단계로 구성되는 실험 또는 조사를 통한 개체의 반응척도가 계층적 구조를 갖는 복합척도의 범주형 자료를 나타낼 때, 이들 자료를 분석하기 위한 모형으로 조건부 지분변수를 이용한 연속모형을 제시하고 있다.

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2009년도 춘계학술대회
• /
• pp.177-183
• /
• 2009

The research develops random ordering methodology of experimental design by the use of nested factor, block factor and repetition types. The spreadsheets developed are useful for quality practioner to acquire the experimental characteristics according to the random ordering.