Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Development of Rapid Detection Technique for Canine Distemper Virus

개 디스템퍼 바이러스의 신속검출법 개발

  • Kim, Doo (Department of Veterinary Medicine, Kangwon National University) ;
  • Ahn, So-Jeo (Department of Veterinary Medicine, Kangwon National University) ;
  • Kwon, Hyuk-Moo (Department of Veterinary Medicine, Kangwon National University)
  • 김두 (강원대학교 수의학과) ;
  • 안소저 (강원대학교 수의학과) ;
  • 권혁무 (강원대학교 수의학과)
  • Published : 2000.06.01
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Abstract

Recently cases of canine distemper have occurred in Korea despite vaccination was carried out nationwidely. This study was performed to establish rapid diagnosis of canine distemper by RT-PCR, nested PCR, and serological test. A total of 30 dogs, which were suspected canine distemper clinically, was examined. RT-PCR and nested PCR were specific for the amplification of CDV H gene and sensitive to detect 7 TCID50 of Onderstepoort strain. By RT-PCR, H gene was detected in 6(20%) of 30 peripheral bloods from dogs. And H gene was detected in 10(33.3%) of 30 samples by nested PCR. H gene was detected from 1 brain of 6 years-old Beagle dog and 1 lung of 2 months-old Shihtzu dog, in which peripheral blood H gene was not detected. Serum neutralizing antibody titer against Onderstepoort strain ranged from 4 to 1,024 in 30 patients. No correlation was observed between the results of nested PCR and titiers of neutralizing antibody.

Keywords

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