• BMB Reports
• /
• v.28 no.2
• /
• pp.170-176
• /
• 1995

Rhodanese from mouse liver was purified to near homogeneity by ammonium sulfate precipitation, CM-Sephadex ion exchange, hydroxyapatite and Sephacryl S-200-HR gel filtration chromatographies with a purification of 776 folds. The molecular weight was determined by Sephadex G-150 gel filtration and found to be 34.8 KDa. SOS-PAGE showed molecular weight 34 KDa and two identical subunits splitting by aging for 3 weeks at $-70^{\circ}C$ the molecular weight of which was 17 KDa. The optimal pH of enzyme activity was 9.4 and the pI value of the enzyme was 6.6. Rhodanese showed the optimal reaction temperature of $25^{\circ}C$ and near linear increasing pattern until 10 min. incubation. $K_m$ values of rhodanese for KCN and $Na_{2}S_{2}O_{3}$ as substrates were 12.5 mM and 8.3 mM, respectively. Rhodanese activity was inhibited by more than 70% at a concentration of 100 ${\mu}M$ of $Ni^{2+}$, $Zn^{2+}$, $Cd^{2+}$, $Hg^{2+}$ and $Cu^{2+}$. Other metal ions, such as $Mn^{2+}$, $Mg^{2-}$, $Ca^{2+}$, and $Fe^{2+}$ showed no effect on rhodanese activity.

• IE interfaces
• /
• v.10 no.2
• /
• pp.1-13
• /
• 1997

The problem of the formation of machine-part cells in FMS is a very important issue at the planning and operating stages of FMS. This problem is inherently a combinatorial optimization problem, proven to be NP-complete(or, NP-hard). Among the several kinds of approaches which have been applied to solve the combinatorial optimization problems, the Simulated Annealing(SA) algorithm, a technique of random search type with a flexibility in generating alternatives, is a powerful problem solving tool. In this paper, the SA algorithm is used to solve machine cell-part family formation problems. The primary purpose of the study is to find the near-optimal solution of machine cell-part family formation problem, whare the product volume and number of operations are prespecified, that can minimize the total material handling cost caused by exceptional elements and intercell moves as much as possible. The results show that the SA algorithm is able to find a near-optimal solution for practical problems of the machine cell-part family formation.

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.811-814
• /
• 2002

Using a mathematical theory, we show that the optimality condition of a turbulent diffuser with maximum pressure recovery at the exit is zero shear stress along the wall. The optimal diffuser shape is designed through iterative procedures by using the $k-{\varepsilon}-{\nu}^{2}-f$ turbulence model for flow simulation. The Reynolds number based on the bulk mean velocity and the channel height at the diffuser entrance is 18,000. We also perform large eddy simulation to validate the shape design results and investigate the flow characteristics near the zero-skin friction wall. Results from large eddy simulation show that the skin friction is slightly higher than zero but is still very small as compared to that of the flat plate boundary layer flow Although the time-averaged wall shear stress is slightly above zero along the diffuser wall, instantaneous flow reversals occur intermittently. The streamwise mein velocity shows an asymptotic behavior of the half-power-law near the wall where the skin friction is close to zero.

• Journal of Biosystems Engineering
• /
• v.38 no.3
• /
• pp.199-207
• /
• 2013

Purpose: Tomatoes, an important agricultural product in fresh-cut markets, are sometimes a source of foodborne illness, mainly Salmonella spp. Growth cracks on tomatoes can be a pathway for bacteria, so its detection prior to consumption is important for public health. In this study, multispectral Visible/Near-Infrared (NIR) reflectance imaging techniques were used to determine optimal wavebands for the classification of defect tomatoes. Methods: Hyperspectral reflectance images were collected from samples of naturally cracked tomatoes. To classify the resulting images, the selected wavelength bands were subjected to two-band permutations, and a supervised classification method was used. Results: The results showed that two optimal wavelengths, 713.8 nm and 718.6 nm, could be used to identify cracked spots on tomato surfaces with a correct classification rate of 91.1%. The result indicates that multispectral reflectance imaging with optimized wavebands from hyperspectral images is an effective technique for the classification of defective tomatoes. Conclusions: Although it can be susceptible to specular interference, the multispectral reflectance imaging is an appropriate method for commercial applications because it is faster and much less expensive than Near-Infrared or fluorescence imaging techniques.

• Progress in Superconductivity
• /
• v.14 no.1
• /
• pp.17-23
• /
• 2012

The heavy-fermion compound $CeRhIn_5$ is a prototypical antiferromagnet where Ce 4f moments align antiferromagnetically below 3.8 K. When doped with Hg, the antiferromagnetic transition $T_N$ initially decreases, becomes flat, and increases again with further increasing Hg concentration. Here we report pressure effects on the electrical resistivity of a 0.45 % Hg-doped $CeRhIn_5$, where $T_N$ is 3.4 K and the magnetic structure is same as that of the undoped compound with Q=(1/2, 1/2, 0.298). With increasing pressure, $T_N$ is suppressed and a superconducting state emerges. The temperature dependence of the electrical resistivity near an optimal pressure shows a power-law behavior that deviates from a $T^2$ dependence, indicating presence of abundant quantum fluctuations near the optimal pressure.

• Transactions on Control, Automation and Systems Engineering
• /
• v.4 no.1
• /
• pp.84-91
• /
• 2002

A new composite control method for a carriage balancing single inverted pendulum system is proposed and applied to swing up the pendulum and to stabilize it under the state constraint. The target inverted pendulum system has an extremely limited length of the cart(below 16cm). The proposed swing-up controller comprises a sliding mode control algorithm and an optimal control algorithm based on two regions: the region near the inverted unstable equilibrium position and the rest of the state space including the downward stable equilibrium position. The sliding mode controller uses a switching control action to converge along the specified path(hyperplane) derived from energy equation from a state around the path to desired state(standing position). An optimal control method is also used to guarantee the stability at unstable equilibrium position. Compared with the reported controllers, it is simpler and easier to implement. Experimental results are given to show the effectiveness of this controller.

• Journal of the Korean Operations Research and Management Science Society
• /
• v.29 no.3
• /
• pp.145-155
• /
• 2004

The design of a communication network has long been a challenging optimization problem. Since the optimal design of a network topology is a well known as a NP-complete problem, many researches have been conducted to obtain near optimal solutions in polynomial time instead of exact optimal solutions. All of these researches suggested diverse heuristic algorithms that can be applied to network design problems. Among these algorithms, a simulated annealing algorithm has been proved to guarantee a good solution for many NP-complete problems. in applying the simulated annealing algorithms to network design problems, generating mechanisms for initial solutions and candidate solutions play an important role in terms of goodness of a solution and efficiency. This study aims at analyzing these mechanisms through experiments, and then suggesting reliable mechanisms.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2002.11b
• /
• pp.771-776
• /
• 2002

Optimal shape of the NFR suspension is studied and developed to improve the dynamic performance and reduce the vibration of the suspension system including a optical head slider. Since accurate position control and stability of the slider motion are highly required in NFR due to the narrower track width and the heavier slider than HDD slider with the low flying height, the dynamic characteristics of the suspension are very important to the mechanical performance of the system. The first natural frequencies in flexural and lateral motion of the suspension are critical factors affecting the dynamics and stability of the flying head, so that the dynamic parameters should be designed properly to avoid an excessive vibration or a crash of the slider on the disk. This paper optimizes the shape of the suspension based on homogenization method in NASTRAN and develops a new suspension shape for NFR system. The suspension is tested on experiment to verify the improvement of the dynamic characteristics.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 1996.04a
• /
• pp.310-313
• /
• 1996

Mathematical programming method for finding optimal solution of job shop scheduling is inadequate to real situation because fo too much computation time. In contrast, dispatching rule is helpful for reducing compuation time but is not guaranted to find optimal solution. The purpose of this paper is to develop a new dispatching rule and procedure to minimize mean flow time whose result is near the optimal solution for job shop scheduling. First step is to select machine which have shortest finishing operation time among the schedulable operations. Second step is to select operation with regard to estimated remaining operation time. The suggested rule is compared with nondelay and MWKR rule for three examples, and is confirmed to be most effective to minimize mean flow time.

• Journal of Electrical Engineering and Technology
• /
• v.12 no.4
• /
• pp.1386-1396
• /
• 2017

In this paper, a new approach is proposed to select the optimal sitting and sizing of distributed solar photovoltaic generation (SPVG) in a radial electrical distribution systems (EDS) considering load/generation uncertainties. Here, distributed generations (DGs) allocation problem is modeled as optimization problem with network loss based objective function under various equality and inequality constrains in an uncertain environment. A boundary power flow is utilized to address the uncertainties in load/generation forecasts. This approach facilitates the consideration of random uncertainties in forecast having no statistical history. Uncertain solar irradiance is modeled by beta distribution function (BDF). The resulted optimization problem is solved by a new Dynamic Harmony Search Algorithm (DHSA). Dynamic band width (DBW) based DHSA is proposed to enhance the search space and dynamically adjust the exploitation near the optimal solution. Proposed approach is demonstrated for two standard IEEE radial distribution systems under different scenarios.