• Journal of Life Science
• /
• v.28 no.6
• /
• pp.681-687
• /
• 2018

Recently, several researchers have been developing cosmetics from natural ingredients for skin whitening and anti-aging products. The red sea cucumber (RSC), Apostichopus japonicas, is a species of sea cucumber in the family stichopodiae, which is widely distributed in China, Japan, and Korea. To use Red Sea Cucumber as a cosmetic ingredient, its inhibitory effects on melanogenesis and the anti-aging effects of RSC extracts were investigated. First, a tyrosinase activity assay was performed, which showed that RSC inhibited tyrosinase activity at a concentration of $200{\mu}g/ml$. An MTT assay was carried out to evaluate cell toxicity, and the results showed that RSC extract has no cytotoxicity in HaCaT cells. Furthermore, the mRNA expression levels of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), microphthalmia-associated transcription factor (MITF), and matrix metalloproteinase (MMPs) genes treated with RSC extract in B16F10 and HaCaT cells decreased. Moreover, a wound-healing assay was performed to identify the cell regeneration effect of RSC extracts. Also, a skin turnover effect was confirmed by creating a three-dimensional cell culture with HaCaT and human fibroblasts. Altogether, the results suggested that Red Sea Cucumber may possess a high ability to induce whitening and anti-wrinkle effects as a cosmeceutical ingredient.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.2
• /
• pp.233-238
• /
• 2002

Moxibustion is one of major healing technique in oriental medicine. It has been widely used in many diseases such as rheumatoid arthritis, Hashimoto disease, breech presentation, etc. However, till now, effects of moxibustion on NK cell activity and relations between sympathetic nerve system(SNS) and the immune alteration induced by moxibustion were not well studied. This study was designed to evaluate effects of moxibustion on NK cell activity and the intervention of SNS in the alteration of NK cell activity induced by moxibustion. Splenic NK cytotoxity was measured in a standard 4-h 51Cr release assay. We measured the NK cytotoxity at after moxibustion stimulation for 1,3,5, and 7 days, and also measured the NK cell cytotoxity after 3 and 7 days burn stimulation with similar temperature. IL-2, IL-4, INF-γ in serum were measured by rat IL-2, IL-4, INF-γ ELISA TEST KIT. To evaluate the effects of sympathectomy on alteration of NK cell cytotoxity, 6-hydroxydopamine(6-OHDA : 5Omg/kg) was used. We showed that NK cell activity of moxibustion stimulation group increased at the 3rd day, and declined at 7th day in comparison with that of contol group. In moxibustion stimulation group, NK cell activity of 3 day stimulation group was significantly higher than sham group. On the contrary, in burn stimulation group, NK cell activity was significantly higher than that of sham groups at 3rd, 7th days. Patterns between moxibustion and burns were different. INF- γ level of 3 days moxibustion stimulation group significantly higher than sham group. IL 2 level among groups were not different. IL-4 was not detected in serum with this method. Sympathectomy abolished the NK cell activity alteration induced by moxibustion. The results suggest that moxibustion induces the alteration of NK cell activity, along with INF-γ and SNS is related to these effects.

• Archives of Plastic Surgery
• /
• v.39 no.2
• /
• pp.118-123
• /
• 2012

Background : An area of the skull exposed by burn injury has been covered by various methods including local flap, skin graft, or free flap surgery. Each method has disadvantages, such as postoperative alopecia or donor site morbidities. Due to the risk of osteomyelitis in the injured skull during the expansion period, tissue expansion was excluded from primary reconstruction. However, successful primary reconstruction was possible in burned skull by tissue expansion. Methods : From January 2000 to 2011, tissue expansion surgery was performed on 10 patients who had sustained electrical burn injuries. In the 3 initial cases, removal of the injured part of the skull and a bone graft was performed. In the latter 7 cases, the injured skull tissue was preserved and covered with a scalp flap directly to obtain natural bone healing and bone remodeling. Results : The mean age of patients was $49.9{\pm}12.2$ years, with 8 male and 2 female. The size of the burn wound was an average of $119.6{\pm}36.7cm^2$. The mean expansion duration was $65.5{\pm}5.6$ days, and the inflation volume was an average of $615{\pm}197.6mL$. Mean defect size was $122.2{\pm}34.9cm^2$. The complications including infection, hematoma, and the exposure of the expander were observed in 4 cases. Nonetheless, only 1 case required revision. Conclusions : Successful coverage was performed by tissue expansion surgery in burned skull primarily and no secondary reconstruction was needed. Although the risks of osteomyelitis during the expansion period were present, constant coverage of the injured skull and active wound treatment helped successful primary reconstruction of burned skull by tissue expansion.

• Journal of Korean society of Dental Hygiene
• /
• v.10 no.2
• /
• pp.283-295
• /
• 2010

Objectives : It surveyed the recognition level of oral health in class teachers who have influence of students' oral health knowledges and behaviors in our country, developed a program available for supplementing reinforcement in school oral health education, and planned the knowledge contents necessary for school oral health education hereafter. Methods : To arrange the basic data necessary for school oral health program, it carried out questionnaire survey by personal self-administration targeting 460 teachers for 19 elementary schools where are located in Jinhae city from May 7, 2007 to June 13. The following conclusions were obtained. Results : 1. The path of acquiring oral health knowledge was shown the high frequency with 23.9% and 23.3%, respectively, through publicity brochure and dental clinic. As for the opinion about the aim of maintaining dental hygiene, the prevention of dental caries was the highest with 53.7%. 2. The opinion about which the regular visit to the dentist is very effective for preventing dental caries accounted for 56.7%. The opinions about which the limit to sugar intake and the use of dental floss are effective for preventing dental caries accounted for 52.2% and 46.3%, respectively. 3. The opinion about which the fluoridation beverage intake and the sealant are effective for preventing dental caries accounted for 33.9% and 33.0%, respectively. 4. The opinion about which the regular toothbrushing without toothpaste has a little effect and has no effect on preventing dental caries accounted for 42.6% and 38.5%, respectively. Conclusions : The elementary-school age period, which is the most core field out of the whole oral health, is the important period that is formed the basis of lifelong oral health care. It surveyed the recognition level of oral health in class teachers who are in charge of direct health care while making continuous life with students, and developed program available for supplementing reinforcement in school oral health education. Through this, there will be necessity for cultivating ability available for properly managing oral health for the whole life by allowing elementary-school students, who will be led to future adults' oral health, to be formed the attitudes toward the rationally oral health care.

• Journal of Families and Better Life
• /
• v.28 no.5
• /
• pp.25-38
• /
• 2010

The purpose of this study was to investigate the trend changes of the health characteristics and the elements of Han-Ok appeared in the articles of the daily newspaper, and to provide basic information for applying to the healthy housing for the public living in modern society and furthermore the various facilities for healing. For the study, the relevant articles of 4 daily newspapers published from 1st of January in 1960 to 28th of February in 2010 were collected and analyzed. The results of the study were as follows: First, the articles about the health characteristics and the elements of Han-Ok have rapidly grown since the year of 2000. Especially, the residents' interviews, case reports and actual living condition of Han-Ok recently tend to increase. Second, the health characteristics of Han-Ok in the recent articles were focused more on the mental and social health than on the physical health. The physical health had the characteristics of the block of hazard environments, the offer of good quality environments, and the enhancements of residents' health. The mental health consisted of the psychological comfort, the sensory satisfaction and the emotional growth. There have been improvements of family relationships and the interactions with neighbors in the characteristics of social health since 2000. Third, the health elements of Han-Ok in terms of architecture and interior design were the structure, space, material, doors & windows, furniture & props, nature, and villages. The material elements were more stressed before 2000, but the spaces and the nature elements were highly focused after 2000 and the structure elements were steadily high regardless of years. Fourth, of the health elements, the structure and the material had the high effects on the physical health characteristics, on the other hand the spaces and the nature influenced more on the mental health. On-dol, Ma-dang, Dae-cheong, Ma-ru, soil, wood, Changhoji, natural environments, and doors & windows were the detail health elements which had high effects on the health characteristics of Han-Ok. For the future study, the experience based in-depth studies of the residents living in Han-Ok are suggested to grasp the actual effects of Han-Ok on the health. The professional consultations are also needed for the extracts of the health characteristics of Han-Ok and the functional elements which can be applied to the modern buildings.

• Journal of Periodontal and Implant Science
• /
• v.24 no.3
• /
• pp.633-648
• /
• 1994

Synthetic bone graft materials have been used for the regeneration of periodontal tissue lost due to periodontal disease, but the limitations of these materials had prompted the use of composite grafts. Among those, a composite graft of calcium carbonate(CC) and calcium sulfate(CS) is one of those materials that has not been studied extensively. CC, which is extracted from a natural coral, is known to possess osteoconductive property. SC can play an adjunctive role in the regeneration of bone tissue, and has shown good resorbability and biocompatibility. This study was conducted in order to investigate the effects of CC and CS composite graft to the regeneration of bone in the intrabony defects of dogs. 3-wall intrabony defects ub size of $4mm{\times}4mm{\times}4mm$ were created in the alveolar bone in the premolar areas. Then those defects that were treated with root planning only were designated as control, while the experimental group 1 and 2 each received the CC and CS composite grafts in the ratio of 8 : 2 and 5 : 5 the animals were sacrificed after 8weeks and the specimens were histologically analyzed. The results were as follows ; 1. No inflammation or foreign body reaction were observed in all subjects. CS has not been seen due to complete resorption, and resorption pattern of CC was observed. 2. Significant differences(p<0.05) in new cementum formation were observed between control($1.42{\pm}0.64mm$) and experimental groups(group 1 ; $2.53{\pm}0.94mm$, group 2 ; $2.23{\pm}0.96mm$) but the difference between the two experimental groups was not significant. 3. Significant differences(p<0.01) in new bone formation were observed between control($0.59{\pm}0.55mm$) and experimental groups(group 1 ; $2.27{\pm}0.61mm$, group 2 ; $2.05{\pm}0.56mm$) but the difference between the two experimental groups was not significant. 4. The extent of apical epithelial migration has shown no significant difference between control($1.18{\pm}1.24mm$) and experimental groups(group 1 ; $0.51{\pm}0.54mm$, group 2 ; $0.73{\pm}0.70mm$). 5. The extent of bone formation was generally limited to the extent of cementum formation for all groups, and significant correlation was found in the amount of bone formation and cementum formation in experimental group 1.(Co.=0.86, p<0.01) These results suggest that the composite graft of CC and CS is biocomplatible and effective in the new bone and new cementum formations. In the case of 3-wall intrabony defects of dogs, the composite ratio of 8 : 2 and 5 : 5 had shown no significant differences in the healing.

• Journal of muscle and joint health
• /
• v.17 no.1
• /
• pp.14-24
• /
• 2010

Purpose: The purpose of this study was to investigate the effect of ear reflexotherapy on back pain of working women in middle age. Methods: The quasi-experimental, nonequivalent control group pretest-posttest design was used for the study. Total of 56 participants composed of experimental group (n=26) and control group (n=30), who were working in four hospitals in urban area. The degree of back pain after ear reflexotherapy was measured by VAS. The data were analyzed by frequency, percent, t-test and $X^2$-test. Results: The degree of back pain after ear reflexotherapy in the experimental group was significantly reduced, while there was no difference in control group. There was a significant difference between the experimental group and control group in the degree of back pain after ear reflexotherapy (t=3.702, p=.001). Conclusion: Application of ear reflexotherapy was effective for employed women in middle age and it needs to be further evaluated among the other population.

• The Journal of Internal Korean Medicine
• /
• v.19 no.1
• /
• pp.524-552
• /
• 1998

Purgation therapy has played an important role as a influential remedy from the begining of the Chinese medicine. Especially purgation therapy is raised as the effective remedy on the acute infectious disease in the book of 'Treatise on Febrile Disease'. But It was inclined to cold-nature and available only in the excess syndrome. Nevertheless it is evident that the book has showed an example of this therapy. During the middle age, purgation therapy is classified into several subtype; hydrogogue therapy, laxation with lubricants, purgation with cold-natured drugs and purgation with warm-natured drugs. Comparing with the ancient times, it must be a progression. It was investigated earnestly by a school leaded by Zhang Congzheng. They were not restricted to several diseases, but applied it to the wide range of diseases. They thought as following. 'One is ill from pathogenic factor so that you should eliminate it from the human body'. Hence, they frequently used three major remedies such as diaphoresis, emesis and purgation. In this process, purgation therapy had showed eye-opening progress. But opposition to it was not little. Li Gao was a representative man on the opposite side. He expressed a critical opinion and placed great importance on the genuine energy, the natural healing force. Under his influence, a large number of doctors evaded purgation and put it under taboo. On account of these trend, purgation therapy had took a backward step and retrograded. Therefore cathartics such as Rhei Radix et Rhizoma, Rharbitidis Semen, cold drugs such as Gypsum Fibrosum, etc. had been excluded for preservation of the genuine energy, and came about an obnoxious custom to value only 'tonity deficiency', or 'warm and tonify'. As it had came into fashion to approach most disease from the point of view, purgation therapy was merely fall into a remedy of constipation. After the eighteenth century purgation therapy encountered the new period of rivival. It was introduced by them who strived for the study of Epidemics to the new current of thought, so called '增水行舟'. It was because 온병 was apt to dissipate one's Yin fluid. Therefore purgation therapy of this period was characterized by establishing nourishment Yin and body fluid with or without use of timely purgation of accumulation of heat. From the time of Zhang Congzheng, it was accomplished by Lee Je-ma to the most epoch-making change. He caused an improvement in the use of purgation therapy by regarding innate constitutional contradiction as importance than representing clinical symptoms. He warned that existing remedies that depend only upon symptoms and signs, not upon individual characteristics including constitutional features didn't bring round to but kill them. And he understood all the pathologic processes in his constitutional theory, investigated specific drugs on four constitution, made indications of each prescriptions clear. For giving to differentiation of constition before differentiation of syndrom, his new slant on the pathologic phenomena overcome the limitations of 변증시치, and revaluate purgation therapy from remedy impaire the genuine energy to that restore it by recover the balance between the internal organ. It is the product of him to fundamentally upset the cause to be in disregard of purgation therapy.

• Journal of the Korean Society of Radiology
• /
• v.5 no.6
• /
• pp.391-400
• /
• 2011

Pneumonia is an infection of the lungs and respiratory system and can be classified by a variety of factors such as infectious agents, etiology, infection area, and other criteria. From a 46-year-old male, who was suspected of being infected with atypical pathogen pneumonia and underwent such tests as serological testing, examination of sputum, urine examination, parasite examination, bronchoscopy, needle biopsy and so on, no significant abnormality was found. This patient also showed no specific symptoms like auscultatory abnormalities, high fever, nonproductive cough, muscle stiffness, sputum production, dyspnea. Prescription of broad-spectrum oral antibiotics and ant-parasitic didn't seem to be effective against bacterial and atypical pathogen. The patient's condition alternately repeated between natural cure and recurrence. The average healing process during which scarring, nodule recurrence and disappearance on the lungs happened was about 20 days. Chest radiography and chest high resolution computerized tomographic scans(HRCT scan) was performed to depict parenchymal aberrations and demarcate the extent and distribution of atypical pathogen pneumonia. As a result, chest radiography did not show the specific symptoms, whereas areas of opacity (seen as white) which represent consolidation were revealed in chest HRCT scan. This indicates that only chest radiography is not that useful for early diagnosis of atypical pathogen pneumonia in patients, since it can't show exactly what the symptom is because of the barriers such as diaphragm, liver, and spine. Therefore, it is desirable that chest HRCT should be used in the diagnosis to compare with the results of chest radiography. Here, report with literature investigations the case of recurrent atypical pathogen pneumonia.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.36 no.4
• /
• pp.591-596
• /
• 2009

In the case of the impacted teeth, the clinician has to consider development of tooth, site of impaction, eruption path, and cooperation of patient. Treatment options for the management of impacted teeth are separated into four categories: observation, intervention, orthodontic or surgical relocation and extraction. Autotransplantation may be defined as the transplantation of embedded, impacted or erupted teeth, from one site to another in the same individual into extraction site or surgically prepared sockets. Autotransplantation ensures preservation of natural tooth, induction of alveolar bone growth and root development, offers one of the fastest and most economically feasible means in the replacement of young patients' missing teeth. This case presents a malpositioned impacted mandibular premolar of an 11-year-old girl. It was thought that orthodontic traction was difficult because of its unfavorable impacted position. Therefore the tooth was treated by autotransplantation, we can observe good healing pattern during 12 months.