Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Whitening Effect and Skin Regeneration Effect of Red Sea Cucumber Extract

홍해삼 추출물의 멜라닌 형성 억제를 통한 미백효과 및 피부 재생효과에 관한 연구

  • Jeon, Mi Ji (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Eun Ji (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Geun Tae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Ga Yeon (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Lee, Seung Jae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Jung, In Cheol (Shinhan ECO Co., Ltd.) ;
  • Kim, Sang-Yong (Department of Food Science & Bio Technology, Shinansan University) ;
  • Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
  • 전미지 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김은지 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • ;
  • 김가연 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 이승제 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 정인철 ((주)신한에코) ;
  • 김상용 (신안산대학교 식품생명학과) ;
  • 김영민 (한남대학교 생명나노과학대학 생명시스템과학과)
  • Received : 2018.02.19
  • Accepted : 2018.06.01
  • Published : 2018.06.30
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Abstract

Recently, several researchers have been developing cosmetics from natural ingredients for skin whitening and anti-aging products. The red sea cucumber (RSC), Apostichopus japonicas, is a species of sea cucumber in the family stichopodiae, which is widely distributed in China, Japan, and Korea. To use Red Sea Cucumber as a cosmetic ingredient, its inhibitory effects on melanogenesis and the anti-aging effects of RSC extracts were investigated. First, a tyrosinase activity assay was performed, which showed that RSC inhibited tyrosinase activity at a concentration of $200{\mu}g/ml$. An MTT assay was carried out to evaluate cell toxicity, and the results showed that RSC extract has no cytotoxicity in HaCaT cells. Furthermore, the mRNA expression levels of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), microphthalmia-associated transcription factor (MITF), and matrix metalloproteinase (MMPs) genes treated with RSC extract in B16F10 and HaCaT cells decreased. Moreover, a wound-healing assay was performed to identify the cell regeneration effect of RSC extracts. Also, a skin turnover effect was confirmed by creating a three-dimensional cell culture with HaCaT and human fibroblasts. Altogether, the results suggested that Red Sea Cucumber may possess a high ability to induce whitening and anti-wrinkle effects as a cosmeceutical ingredient.

홍해삼 또는 Apostichopus japonicas는 동남아시아에서 발견되는 stichopodiae의 한 종이다. 본 연구에서는 홍해삼의 화장품 소재로서 사용가능한지 알아보기 위해 홍해삼 추출물의 미백, 항주름에 관한 실험을 진행하였다. tyrosinase 활성 분석 결과, 홍해삼 추출물 $200{\mu}g/ml$에서 tyrosinase 활성을 억제하였다. 또한, 홍해삼은 tyrosinase, TRP-1, TRP-2, MITF 및 matrix metalloproteinase (MMPs)의 mRNA 발현을 억제하였다. 이어서 HaCaT과 human Fibroblast를 이용한 3차원 세포배양을 통해 홍해삼의 피부 턴오버 주기 개선효과를 검증하였다. 이러한 결과를 바탕으로 홍해삼이 높은 미백효과 및 주름개선효과를 가지는 화장품 소재로서 충분한 가치를 지닐 것으로 판단된다.

Keywords

References

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